LOCUS       pXYB111_35S_UTR_GFPuv_ 14233 bp ds-DNA     circular     25-SEP-2023
DEFINITION  .
KEYWORDS    "creator:Xiaohan Yang" "marker:NeoR/KanR, KanR, Modified
            COR47-5'-UTR"
COMMENT     pGFPuv difested by PacI and SbfI + gYL091
FEATURES             Location/Qualifiers
     promoter        complement(16..361)
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     738..754
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     misc_feature    745..774
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    745..1756
                     /label="gyl091"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        1269..1614
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     misc_feature    1599..1614
                     /label="overlap1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1615..1721
                     /label="Modified COR47-5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..1726
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..3046
                     /label="gYL098"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1727..1756
                     /label="GIBSON OVERHANG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1730..1754
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1730..2389
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTTFKIESRIHGNLNGEKFELVGGGVGEEGRLEIEMKTKDKPLAFSPFLLTTCMGYGFYHFASFPKGIKNIYLHAATNGGYTNTRKEIYEDGGILEVNFRYTYEFNKIIGDVECIGHGFPSQSPIFKDTIVKSCPTVDLMLPMSGNIIASSYAYAFQLKDGSFYTAEVKNNIDFKNPIHESFSKSGPMFTHRRVEETLTKENLAIVEYQQVFNSAPRDM*"
     misc_feature    1730..2389
                     /label="eYGFPuv"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1760..1784
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2290..2314
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2390..2499
                     /label="triplex"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2500..3377
                     /label="HSPT878"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2525..2549
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3011..3046
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3378..3398
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3429..3453
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(3439..3455)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    3463..3479
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3487..3493)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3494..3511)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3512..3517)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3532..3553
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        3744..4421
                     /label="CaMV 35S promoter (enhanced)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
     misc_feature    4425..5351
                     /label="gBlocks for change to Kan"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4489..5286
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5326..5500
                     /label="CaMV poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: cauliflower mosaic virus polyadenylation signal"
     misc_feature    5578..5602
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             6027..6821
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aphA-3 note: confers resistance to kanamycin product: aminoglycoside phosphotransferase protein_id: transl_table: 1 translation: MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
     rep_origin      6908..7496
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    7682..7822
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     rep_origin      8166..8360
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(8426..9493)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 note: product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) protein_id: transl_table: 1 translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(9927..10556)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) transl_table: 1 translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     misc_feature    11856..11880
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     terminator      11902..12154
                     /label="NOS terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: nopaline synthase terminator and poly(A) signal"
     CDS             complement(12186..12203)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     CDS             complement(12210..14027)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14028..14217
                     /label="cat1 intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: castor bean catalase intron, modified"
     CDS             complement(14028..14217)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             complement(14218..14232)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacaCAAACA
     1621 TTACTCATTC ACAAAACCAT CTTAAAGCAA CTACACAAGT CTTGAAATTT TCTCATATTT
     1681 TCTATTTACT ATATAAACTT TTAATCAAAT CAAGATTAAA GTTAATTAAa tgacaacctt
     1741 caaaatcgag tcccggatcc acggcaacct caacggggag aagttcgagt tggttggagg
     1801 tggagtaggt gaggagggtc gcctcgagat tgagatgaag actaaagata aaccactggc
     1861 attctctccc ttcctgctga ccacttgcat gggttacggg ttctaccact tcgccagctt
     1921 cccaaagggg attaagaaca tctatcttca tgctgcaacg aacggaggtt acaccaacac
     1981 caggaaggag atctatgaag acggcggcat cttggaggtc aacttccgtt acacttacga
     2041 gttcaacaag atcatcggtg acgtcgagtg cattggacat ggattcccaa gtcagagtcc
     2101 gatcttcaag gacacgatcg tgaagagttg tcccacggtg gacctgatgt tgccaatgtc
     2161 cgggaacatc atcgccagct cctacgctta cgccttccaa ctgaaggacg gctctttcta
     2221 cacggcagaa gtcaagaaca acatagactt caagaatcca atccacgagt ccttctcgaa
     2281 gtcagggccc atgttcaccc acagacgtgt cgaggagact ctcaccaagg agaaccttgc
     2341 catagtggag taccagcagg ttttcaacag cgccccaaga gacatgtagG ATTCGTCAGT
     2401 AGGGTTGTAA AGGTTTTTCT TTTCCTGAGA AAACAACCTT TTGTTTTCTC AGGTTTTGCT
     2461 TTTTGGCCTT TCCCTAGCTT TAAAAAAAAA AAAGCAAAAA TATGAAGATG AAGATGAAAT
     2521 ATTTGGTGTG TCAAATAAAA AGCTTGTGTG CTTAAGTTTG TGTTTTTTTC TTGGCTTGTT
     2581 GTGTTATGAA TTTGTGGCTT TTTCTAATAT TAAATGAATG TAAGATCTCA TTATAATGAA
     2641 TAAACAAATG TTTCTATAAT CCATTGTGAA TGTTTTGTTG GATCTCTTCT GCAGCATATA
     2701 ACTACTGTAT GTGCTATGGT ATGGACTATG GAATATGATT AAAGATAAGA TGGGCTCATA
     2761 GAGTAAAACG AGGCGAGGGA CCTATAAACC TCCCTTCATC ATGCTATTTC ATGATCTATT
     2821 TTATAAAATA AAGATGTAGA AAAAAGTAAG CGTAATAACC GCAAAACAAA TGATTTAAAA
     2881 CATGGCACAT AATGAGGAGA TTAAGTTCGG TTTACGTTTA TTTTAGTACT AATTGTAACG
     2941 TGAGACTACG TATCGGGAAT CGCCTAATTA AAGCATTAAT GCGAACCTGA TTAGATTCAC
     3001 CGACCCTCCT ATCGTGTCGA CCTTTCTGTT TCTTAGAATT TTTTGGTAGT CTATGTACTA
     3061 ATAATGTCAG CTTCGTATTT ATTTCATAAG CAATTTGCAT TTGCAATTTG TTTTTTACTT
     3121 TTATTTTTAT TGTATTGTGG AATGTGGACT CGTACCAACA TGAAGTTATA TACCACCAAA
     3181 AAAATTACAG TTAGTCAAAA GATTCACGAG TGAGAGCTAC TTATGATTGT CTTTTACGTA
     3241 TATGTCTAAT TGTCTATTTG CTCAATAATC TTTGTACTTT CTTTTGTCGT TGATAAAATC
     3301 ACAAAGTTCC AAAAGTAATC GAATGATTTG CTTTTAAGAA AAGAAGAGCT CAATAATTCA
     3361 ACATATATCT GTACACAatt accgcctttg agtgagctct agatgccacc tgacgtctaa
     3421 gaagaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3481 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3541 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3601 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag
     3661 cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     3721 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     3781 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     3841 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     3901 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     3961 ccaaccacgt cttcaaagca agtggattga tgtgataaca tggtggagca cgacactctc
     4021 gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt
     4081 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     4141 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
     4201 aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
     4261 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     4321 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agaccttcct
     4381 ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta
     4441 caaatctatc tctctcgagc tttcgcagat cccggggggc aatgagatat ggggattgaa
     4501 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4561 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4621 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact ccaggacgag
     4681 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4741 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4801 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4861 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4921 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4981 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5041 ctcgtcgtga cacatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5101 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5161 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5221 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5281 ttctgaagta gatgccgacc ggatctgtcg atcgacaagc tcgagtttct ccataataat
     5341 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
     5401 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     5461 ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta attcggcgtt
     5521 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     5581 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     5641 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     5701 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     5761 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     5821 agcgttgctg cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac
     5881 gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca
     5941 aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac
     6001 tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa
     6061 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
     6121 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
     6181 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
     6241 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
     6301 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
     6361 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
     6421 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
     6481 ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt
     6541 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
     6601 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
     6661 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata
     6721 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     6781 agaaaataaa atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca
     6841 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6901 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6961 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7021 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7081 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7141 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7201 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7261 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7321 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7381 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7441 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7501 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7561 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7621 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7681 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     7741 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     7801 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     7861 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     7921 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
     7981 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta
     8041 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
     8101 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
     8161 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
     8221 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
     8281 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
     8341 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa
     8401 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
     8461 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
     8521 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
     8581 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
     8641 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
     8701 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
     8761 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
     8821 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
     8881 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
     8941 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
     9001 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
     9061 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
     9121 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
     9181 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
     9241 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
     9301 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
     9361 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
     9421 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
     9481 atggtttgcg accgtcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc
     9541 cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat
     9601 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa
     9661 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga
     9721 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc
     9781 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa
     9841 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt
     9901 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc
     9961 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg
    10021 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg
    10081 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac
    10141 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc
    10201 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag
    10261 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact
    10321 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg
    10381 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga
    10441 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt
    10501 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt
    10561 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca
    10621 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg
    10681 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca
    10741 gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg
    10801 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc
    10861 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc
    10921 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag
    10981 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg
    11041 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg
    11101 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat
    11161 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc
    11221 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg
    11281 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt
    11341 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc
    11401 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc
    11461 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc
    11521 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt
    11581 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg
    11641 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa
    11701 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc
    11761 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
    11821 tatccgttat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
    11881 aacactgata gtttaattcc cgatctagta acatagatga caccgcgcgc gataatttat
    11941 cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc
    12001 taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct
    12061 taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc
    12121 ttaagaaact ttattgccaa atgtttgaac gatcggggaa attcgagctg gtcaccaatt
    12181 cacacgtgat ggtgatggtg atggctagcg ttcttgtagc cgaaatctgg aatgttggtc
    12241 cagcgctcgc gaaagacgtg cgcggcgagc ttcggcttgc ggtcacgagt gaacacgccc
    12301 ttcttgtttc cttggacgcg catcacgccc tgagaggtcg cgaagtccgc gaagttccac
    12361 gcttgctcac ccacgaagtt ctcaaactca tcgaacacga cgtggttcgc ctggtagtac
    12421 tcgacttgat attcctcggt gaacatcact ggatcaatgt cgtgaaagcc cgcaacggtg
    12481 tctgcgccgt actcagtgat catgatcggc tttcctgggc aacgcttgtt ccacgcgtga
    12541 aattcctggc ggagatggac tttggccgct tcgagatcac cgccatcgaa gtaccatccg
    12601 ttatagcgat tgagcgcgat gacgtcaatc agttcggcga ctttgtccgt ctccggggta
    12661 gccatcacaa acagcacgat cgtgaccgga cgcttctgtg ggtcgagttc cttggtcagc
    12721 tccaccaacg gcttgaagta ctcgtacgcg ccctcttcct cagtcgccgc ctcgttggcg
    12781 atgctccaca tcacgacgct tggatggttc ttgtcacgag acaccagttc acggagaacg
    12841 tcttgatggt gctcaaacgt ccgaatcttc tcccaggtac tgacgcgctc gctgccttcg
    12901 ccgagtcccg tggtggccat gaagttgagg tgcacgccaa ctgccggagt ctcgtcgatc
    12961 acgaccagac cctcgcgatc cgcaagacgc atcaactctt cagagtacgg atagtgtgcg
    13021 gtccggaagc tgttggcgcc gatccatttg aggatattga aatccatcac attgctcgct
    13081 tcgttaaagc cacggccgtt gataggagtg tcctcatgtt tgccaaagcc cttgaagtag
    13141 aacggtttgt tgttgatgag gaacttgccg tcgttgactt ccacggtccg cacgccgaac
    13201 ggctcttcat agacatcgat ggtcagtccg tcgttcacca gttccacttt gatctggtag
    13261 agatacgtgt tcagtggttc ccagaggatg acattcggaa tctccacgtt accgctcagg
    13321 ccctcggtgc ttgcgaccac tttgccttcc tcatccacga ccgacacttt cacggtctcg
    13381 gctttgcctt gaaagtccac cgtataggtc acagtcccgg ttgggccatt gaagtcggtc
    13441 acaaccgaga tgtcctcgac gtacgtaaac ggggtcgtgt agattttcac cggacggtgc
    13501 aggcctgcat agttgaagaa gtcgaagttc ggcttgttac gaatgacttt tccgaggccc
    13561 tcttcgtggc gctcgctgta cagccccacc gggagggtgc tatcgtcgag gatgttgtcc
    13621 acggcgacgg tgacgcgatt catgccatca cgcagcgagt tgttgatttc cgcttcgaat
    13681 ggcaggaatc cgcccttgtg ctccacgacc agctcaccat tgacatagac aattgctttg
    13741 tgagttgcag agccgaagcg gagcacgata cgctgatcct tcagataggc cggcaccgtg
    13801 aactcacgtt cgtaccagac atatccgata tggttgcgga tttccttggt cacgccaatg
    13861 tcattgtaac tgcttgggac ggccatacta atagtgtcgg tcagcttgct ttcgtaccac
    13921 ttctcttcca gtcctttccc gtagtccagc ttgaagttcc agacgccatt gaggtcgaag
    13981 acgccacggg tctcggtgtt gatcgggtac agactagttc gtcggttctg taactatcat
    14041 catcatcata gacacacgaa ataaagtaat cagattatca gttaaagcta tgtaatattt
    14101 acaccataac caatcaatta aaaaatagat cagtttaaag aaagatcaaa gctcaaaaaa
    14161 ataaaaagag aaaagggtcc taaccaagaa aatgaaggag aaaaactaga aatttaccct
    14221 cagatctacc atg
//