LOCUS       pXYB112_Ribozyme_split 14646 bp ds-DNA     circular     15-NOV-2023
DEFINITION  .
KEYWORDS    "creator:Xiaohan Yang" "marker:NeoR/KanR, KanR, Modified
            COR47-5'-UTR"
COMMENT     pXYB028 difested by PacI and SbfI+gYL091
FEATURES             Location/Qualifiers
     promoter        complement(16..361)
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     738..754
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     primer          738..754
                     /label="oYL_ORNL048"
                     /note="sequence: gtaaaacgacggccagt"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    745..774
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    745..1756
                     /label="gYL091"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(773..790)
                     /label="oYL_ORNL_047"
                     /note="sequence: ggctaatctggggacctg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1269..1614
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     misc_feature    1599..1614
                     /label="overlap1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1615..1721
                     /label="Modified COR47-5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..1726
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..3459
                     /label="gYL099"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1727..1756
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1730..1754
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1730..1945
                     /label="1st half GFPuv (Y72)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1730..2802
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTTFKIESRIHGNLNGEKFELVGGGVGEEGRLEIEMKTKDKPLAFSPFLLTTCMGYGFYHFASFPKGIKNIYK*QYLPLGQKVIRHAPGS*SLNQ*IASV*KARPSNCGKGVNSRSVPSLRGNFEMALQRVW**ADGHGPNHAAKS*VNRSSVDMDAVHRLNVGRGRCILLIRYSRTSP*WELADEVMQHWSRWELICMRKYID*FWSTRFMLQRTEVTPTPGRRSMKTAASWRSTSVTLTSSTRSSVTSSALDMDSQVRVRSSRTRS*RVVPRWT*CCQCPGTSSPAPTLTPSN*RTALSTRQKSRTT*TSRIQSTSPSRSQGPCSPTDVSRRLSPRRTLP*WSTSRFSTAPQETC"
     misc_feature    1760..1784
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1819..1840
                     /label="oYL_ORNL138"
                     /note="sequence: tcgcctcgagattgagatgaag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1946..1965
                     /label="P1 Loop WT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1966..1971
                     /label="Internal guide sequence"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1972..2358
                     /label="Ribozyme"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1976..1998)
                     /label="oYL_ORNL135"
                     /note="sequence: CTACCAGGTGCATGCCTGATAAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2359..2802
                     /label="2nd half GFPuv"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          2367..2386
                     /label="oYL_ORNL140"
                     /note="sequence: tgcaacgaacggaggttaca"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2703..2727
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2725..2744)
                     /label="oYL_ORNL139"
                     /note="sequence: tccttggtgagagtctcctc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2803..2912
                     /label="triplex"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2895..2906
                     /label="12A polytail"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2913..3790
                     /label="HSPT878"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2938..2962
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3424..3459
                     /label="Gibson overhang"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3791..3811
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3842..3866
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3848..3868)
                     /label="oYL_ORNL029"
                     /note="sequence: caggaaacagctatgaccatg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3852..3868)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    3876..3892
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3900..3906)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3907..3924)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3925..3930)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3945..3966
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        4157..4834
                     /label="CaMV 35S promoter (enhanced)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
     misc_feature    4838..5764
                     /label="gBlocks for change to Kan"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4902..5699
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5739..5913
                     /label="CaMV poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: cauliflower mosaic virus polyadenylation signal"
     misc_feature    5991..6015
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             6440..7234
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aphA-3 note: confers resistance to kanamycin product: aminoglycoside phosphotransferase protein_id: transl_table: 1 translation: MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
     rep_origin      7321..7909
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    8095..8235
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     rep_origin      8579..8773
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(8839..9906)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 note: product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) protein_id: transl_table: 1 translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(10340..10969)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) transl_table: 1 translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     misc_feature    12269..12293
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     terminator      12315..12567
                     /label="NOS terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: nopaline synthase terminator and poly(A) signal"
     CDS             complement(12599..12616)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     CDS             complement(12623..14440)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14441..14630
                     /label="cat1 intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: castor bean catalase intron, modified"
     CDS             complement(14441..14630)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             complement(14631..14645)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacaCAAACA
     1621 TTACTCATTC ACAAAACCAT CTTAAAGCAA CTACACAAGT CTTGAAATTT TCTCATATTT
     1681 TCTATTTACT ATATAAACTT TTAATCAAAT CAAGATTAAA GTTAATTAAa tgacaacctt
     1741 caaaatcgag tcccggatcc acggcaacct caacggggag aagttcgagt tggttggagg
     1801 tggagtaggt gaggagggtc gcctcgagat tgagatgaag actaaagata aaccactggc
     1861 attctctccc ttcctgctga ccacttgcat gggttacggg ttctaccact tcgccagctt
     1921 cccaaagggg attaagaaca tctatAAATA GCAATATTTA CCTTTGGGTC AAAAAGTTAT
     1981 CAGGCATGCA CCTGGTAGCT AGTCTTTAAA CCAATAGATT GCATCGGTTT AAAAGGCAAG
     2041 ACCGTCAAAT TGCGGGAAAG GGGTCAACAG CCGTTCAGTA CCAAGTCTCA GGGGAAACTT
     2101 TGAGATGGCC TTGCAAAGGG TATGGTAATA AGCTGACGGA CATGGTCCTA ACCACGCAGC
     2161 CAAGTCCTAA GTCAACAGAT CTTCTGTTGA TATGGATGCA GTTCACAGAC TAAATGTCGG
     2221 TCGGGGAAGA TGTATTCTTC TCATAAGATA TAGTCGGACC TCTCCTTAAT GGGAGCTAGC
     2281 GGATGAAGTG ATGCAACACT GGAGCCGCTG GGAACTAATT TGTATGCGAA AGTATATTGA
     2341 TTAGTTTTGG AGTACTCGct tcatgctgca acgaacggag gttacaccaa caccaggaag
     2401 gagatctatg aagacggcgg catcttggag gtcaacttcc gttacactta cgagttcaac
     2461 aagatcatcg gtgacgtcga gtgcattgga catggattcc caagtcagag tccgatcttc
     2521 aaggacacga tcgtgaagag ttgtcccacg gtggacctga tgttgccaat gtccgggaac
     2581 atcatcgcca gctcctacgc ttacgccttc caactgaagg acggctcttt ctacacggca
     2641 gaagtcaaga acaacataga cttcaagaat ccaatccacg agtccttctc gaagtcaggg
     2701 cccatgttca cccacagacg tgtcgaggag actctcacca aggagaacct tgccatagtg
     2761 gagtaccagc aggttttcaa cagcgcccca agagacatgt agGATTCGTC AGTAGGGTTG
     2821 TAAAGGTTTT TCTTTTCCTG AGAAAACAAC CTTTTGTTTT CTCAGGTTTT GCTTTTTGGC
     2881 CTTTCCCTAG CTTTAAAAAA AAAAAAGCAA AAATATGAAG ATGAAGATGA AATATTTGGT
     2941 GTGTCAAATA AAAAGCTTGT GTGCTTAAGT TTGTGTTTTT TTCTTGGCTT GTTGTGTTAT
     3001 GAATTTGTGG CTTTTTCTAA TATTAAATGA ATGTAAGATC TCATTATAAT GAATAAACAA
     3061 ATGTTTCTAT AATCCATTGT GAATGTTTTG TTGGATCTCT TCTGCAGCAT ATAACTACTG
     3121 TATGTGCTAT GGTATGGACT ATGGAATATG ATTAAAGATA AGATGGGCTC ATAGAGTAAA
     3181 ACGAGGCGAG GGACCTATAA ACCTCCCTTC ATCATGCTAT TTCATGATCT ATTTTATAAA
     3241 ATAAAGATGT AGAAAAAAGT AAGCGTAATA ACCGCAAAAC AAATGATTTA AAACATGGCA
     3301 CATAATGAGG AGATTAAGTT CGGTTTACGT TTATTTTAGT ACTAATTGTA ACGTGAGACT
     3361 ACGTATCGGG AATCGCCTAA TTAAAGCATT AATGCGAACC TGATTAGATT CACCGACCCT
     3421 CCTATCGTGT CGACCTTTCT GTTTCTTAGA ATTTTTTGGT AGTCTATGTA CTAATAATGT
     3481 CAGCTTCGTA TTTATTTCAT AAGCAATTTG CATTTGCAAT TTGTTTTTTA CTTTTATTTT
     3541 TATTGTATTG TGGAATGTGG ACTCGTACCA ACATGAAGTT ATATACCACC AAAAAAATTA
     3601 CAGTTAGTCA AAAGATTCAC GAGTGAGAGC TACTTATGAT TGTCTTTTAC GTATATGTCT
     3661 AATTGTCTAT TTGCTCAATA ATCTTTGTAC TTTCTTTTGT CGTTGATAAA ATCACAAAGT
     3721 TCCAAAAGTA ATCGAATGAT TTGCTTTTAA GAAAAGAAGA GCTCAATAAT TCAACATATA
     3781 TCTGTACACA attaccgcct ttgagtgagc tctagatgcc acctgacgtc taagaagaat
     3841 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3901 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3961 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4021 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg
     4081 ccaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
     4141 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
     4201 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
     4261 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
     4321 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
     4381 cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacact ctcgtctact
     4441 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
     4501 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
     4561 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
     4621 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     4681 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     4741 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagacctt cctctatata
     4801 aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct ctacaaatct
     4861 atctctctcg agctttcgca gatcccgggg ggcaatgaga tatggggatt gaacaagatg
     4921 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     4981 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5041 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaggac gaggcagcgc
     5101 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5161 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5221 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5281 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     5341 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     5401 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     5461 tgacacatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     5521 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     5581 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     5641 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa
     5701 gtagatgccg accggatctg tcgatcgaca agctcgagtt tctccataat aatgtgtgag
     5761 tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata
     5821 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     5881 ctaaaaccaa aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag
     5941 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     6001 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     6061 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     6121 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     6181 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     6241 ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact
     6301 ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca
     6361 gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa
     6421 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
     6481 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     6541 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     6601 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     6661 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     6721 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     6781 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     6841 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
     6901 ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt
     6961 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
     7021 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
     7081 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga
     7141 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
     7201 aaaatattat attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc
     7261 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7321 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7381 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7441 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     7501 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7561 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7621 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7681 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7741 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7801 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7861 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7921 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7981 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8041 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     8101 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     8161 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     8221 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8281 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8341 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     8401 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     8461 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     8521 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     8581 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     8641 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     8701 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     8761 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     8821 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     8881 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     8941 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     9001 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     9061 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     9121 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     9181 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     9241 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     9301 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     9361 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     9421 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     9481 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     9541 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     9601 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     9661 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     9721 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     9781 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     9841 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     9901 gcgaccgtca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     9961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
    10021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
    10081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
    10141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
    10201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
    10261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
    10321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
    10381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
    10441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
    10501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
    10561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
    10621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
    10681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
    10741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
    10801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
    10861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
    10921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
    10981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
    11041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
    11101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
    11161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
    11221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
    11281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
    11341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
    11401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
    11461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
    11521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
    11581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
    11641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
    11701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
    11761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
    11821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
    11881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
    11941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
    12001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
    12061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
    12121 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
    12181 tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccgt
    12241 tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg
    12301 atagtttaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
    12361 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
    12421 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
    12481 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
    12541 actttattgc caaatgtttg aacgatcggg gaaattcgag ctggtcacca attcacacgt
    12601 gatggtgatg gtgatggcta gcgttcttgt agccgaaatc tggaatgttg gtccagcgct
    12661 cgcgaaagac gtgcgcggcg agcttcggct tgcggtcacg agtgaacacg cccttcttgt
    12721 ttccttggac gcgcatcacg ccctgagagg tcgcgaagtc cgcgaagttc cacgcttgct
    12781 cacccacgaa gttctcaaac tcatcgaaca cgacgtggtt cgcctggtag tactcgactt
    12841 gatattcctc ggtgaacatc actggatcaa tgtcgtgaaa gcccgcaacg gtgtctgcgc
    12901 cgtactcagt gatcatgatc ggctttcctg ggcaacgctt gttccacgcg tgaaattcct
    12961 ggcggagatg gactttggcc gcttcgagat caccgccatc gaagtaccat ccgttatagc
    13021 gattgagcgc gatgacgtca atcagttcgg cgactttgtc cgtctccggg gtagccatca
    13081 caaacagcac gatcgtgacc ggacgcttct gtgggtcgag ttccttggtc agctccacca
    13141 acggcttgaa gtactcgtac gcgccctctt cctcagtcgc cgcctcgttg gcgatgctcc
    13201 acatcacgac gcttggatgg ttcttgtcac gagacaccag ttcacggaga acgtcttgat
    13261 ggtgctcaaa cgtccgaatc ttctcccagg tactgacgcg ctcgctgcct tcgccgagtc
    13321 ccgtggtggc catgaagttg aggtgcacgc caactgccgg agtctcgtcg atcacgacca
    13381 gaccctcgcg atccgcaaga cgcatcaact cttcagagta cggatagtgt gcggtccgga
    13441 agctgttggc gccgatccat ttgaggatat tgaaatccat cacattgctc gcttcgttaa
    13501 agccacggcc gttgatagga gtgtcctcat gtttgccaaa gcccttgaag tagaacggtt
    13561 tgttgttgat gaggaacttg ccgtcgttga cttccacggt ccgcacgccg aacggctctt
    13621 catagacatc gatggtcagt ccgtcgttca ccagttccac tttgatctgg tagagatacg
    13681 tgttcagtgg ttcccagagg atgacattcg gaatctccac gttaccgctc aggccctcgg
    13741 tgcttgcgac cactttgcct tcctcatcca cgaccgacac tttcacggtc tcggctttgc
    13801 cttgaaagtc caccgtatag gtcacagtcc cggttgggcc attgaagtcg gtcacaaccg
    13861 agatgtcctc gacgtacgta aacggggtcg tgtagatttt caccggacgg tgcaggcctg
    13921 catagttgaa gaagtcgaag ttcggcttgt tacgaatgac ttttccgagg ccctcttcgt
    13981 ggcgctcgct gtacagcccc accgggaggg tgctatcgtc gaggatgttg tccacggcga
    14041 cggtgacgcg attcatgcca tcacgcagcg agttgttgat ttccgcttcg aatggcagga
    14101 atccgccctt gtgctccacg accagctcac cattgacata gacaattgct ttgtgagttg
    14161 cagagccgaa gcggagcacg atacgctgat ccttcagata ggccggcacc gtgaactcac
    14221 gttcgtacca gacatatccg atatggttgc ggatttcctt ggtcacgcca atgtcattgt
    14281 aactgcttgg gacggccata ctaatagtgt cggtcagctt gctttcgtac cacttctctt
    14341 ccagtccttt cccgtagtcc agcttgaagt tccagacgcc attgaggtcg aagacgccac
    14401 gggtctcggt gttgatcggg tacagactag ttcgtcggtt ctgtaactat catcatcatc
    14461 atagacacac gaaataaagt aatcagatta tcagttaaag ctatgtaata tttacaccat
    14521 aaccaatcaa ttaaaaaata gatcagttta aagaaagatc aaagctcaaa aaaataaaaa
    14581 gagaaaaggg tcctaaccaa gaaaatgaag gagaaaaact agaaatttac cctcagatct
    14641 accatg
//