LOCUS       pXYB113_dRibozyme_spli 14620 bp ds-DNA     circular     25-SEP-2023
DEFINITION  .
KEYWORDS    "creator:Xiaohan Yang" "marker:NeoR/KanR, KanR, Modified
            COR47-5'-UTR"
COMMENT     pXYB031 difested by PacI and SbfI+gYL091
FEATURES             Location/Qualifiers
     promoter        complement(16..361)
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     738..754
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     primer          738..754
                     /label="oYL_ORNL048"
                     /note="sequence: gtaaaacgacggccagt"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    745..774
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    745..1756
                     /label="gYL091"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(773..790)
                     /label="oYL_ORNL_047"
                     /note="sequence: ggctaatctggggacctg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1269..1614
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     misc_feature    1599..1614
                     /label="overlap1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1615..1721
                     /label="Modified COR47-5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..1726
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..3433
                     /label="gYL100"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1727..1756
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1730..1754
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1730..1945
                     /label="1st half GFPuv"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1730..2776
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTTFKIESRIHGNLNGEKFELVGGGVGEEGRLEIEMKTKDKPLAFSPFLLTTCMGYGFYHFASFPKGIKNIYKSYQACTW*LVFKPIDCIGLKGKTVKLRERGQQPFSTKSQGKL*DGLAKGMVIS*RTWS*PRSQVLSQQIFC*YGCSSQTKCRSGKMYSSHKI*SDLSLMGASG*SDATLEPLGTNLYAKVY*LVLEYSLHAATNGGYTNTRKEIYEDGGILEVNFRYTYEFNKIIGDVECIGHGFPSQSPIFKDTIVKSCPTVDLMLPMSGNIIASSYAYAFQLKDGSFYTAEVKNNIDFKNPIHESFSKSGPMFTHRRVEETLTKENLAIVEYQQVFNSAPRDM*"
     misc_feature    1760..1784
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1946..2332
                     /label="dRIbozyme"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2333..2776
                     /label="2nd half GFPuv"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2677..2701
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2777..2886
                     /label="triplex"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2887..3764
                     /label="HSPT878"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2912..2936
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3398..3433
                     /label="Gibson overhang"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3765..3785
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3816..3840
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3822..3842)
                     /label="oYL_ORNL029"
                     /note="sequence: caggaaacagctatgaccatg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3826..3842)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    3850..3866
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3874..3880)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3881..3898)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3899..3904)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3919..3940
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        4131..4808
                     /label="CaMV 35S promoter (enhanced)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
     misc_feature    4812..5738
                     /label="gBlocks for change to Kan"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4876..5673
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5713..5887
                     /label="CaMV poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: cauliflower mosaic virus polyadenylation signal"
     misc_feature    5965..5989
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             6414..7208
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aphA-3 note: confers resistance to kanamycin product: aminoglycoside phosphotransferase protein_id: transl_table: 1 translation: MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
     rep_origin      7295..7883
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    8069..8209
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     rep_origin      8553..8747
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(8813..9880)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 note: product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) protein_id: transl_table: 1 translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(10314..10943)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) transl_table: 1 translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     misc_feature    12243..12267
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     terminator      12289..12541
                     /label="NOS terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: nopaline synthase terminator and poly(A) signal"
     primer          12512..12538
                     /label="oYL072_oNB395 copy"
                     /note="sequence: GAAACTTTATTGCCAAATGTTTGAACG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(12573..12590)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     CDS             complement(12597..14414)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14415..14604
                     /label="cat1 intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: castor bean catalase intron, modified"
     CDS             complement(14415..14604)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             complement(14605..14619)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacaCAAACA
     1621 TTACTCATTC ACAAAACCAT CTTAAAGCAA CTACACAAGT CTTGAAATTT TCTCATATTT
     1681 TCTATTTACT ATATAAACTT TTAATCAAAT CAAGATTAAA GTTAATTAAa tgacaacctt
     1741 caaaatcgag tcccggatcc acggcaacct caacggggag aagttcgagt tggttggagg
     1801 tggagtaggt gaggagggtc gcctcgagat tgagatgaag actaaagata aaccactggc
     1861 attctctccc ttcctgctga ccacttgcat gggttacggg ttctaccact tcgccagctt
     1921 cccaaagggg attaagaaca tctatAAAAG TTATCAGGCA TGCACCTGGT AGCTAGTCTT
     1981 TAAACCAATA GATTGCATCG GTTTAAAAGG CAAGACCGTC AAATTGCGGG AAAGGGGTCA
     2041 ACAGCCGTTC AGTACCAAGT CTCAGGGGAA ACTTTGAGAT GGCCTTGCAA AGGGTATGGT
     2101 AATAAGCTGA CGGACATGGT CCTAACCACG CAGCCAAGTC CTAAGTCAAC AGATCTTCTG
     2161 TTGATATGGA TGCAGTTCAC AAACTAAATG TCGGTCGGGG AAGATGTATT CTTCTCATAA
     2221 GATATAGTCG GACCTCTCCT TAATGGGAGC TAGCGGATGA AGTGATGCAA CACTGGAGCC
     2281 GCTGGGAACT AATTTGTATG CGAAAGTATA TTGATTAGTT TTGGAGTACT CGcttcatgc
     2341 tgcaacgaac ggaggttaca ccaacaccag gaaggagatc tatgaagacg gcggcatctt
     2401 ggaggtcaac ttccgttaca cttacgagtt caacaagatc atcggtgacg tcgagtgcat
     2461 tggacatgga ttcccaagtc agagtccgat cttcaaggac acgatcgtga agagttgtcc
     2521 cacggtggac ctgatgttgc caatgtccgg gaacatcatc gccagctcct acgcttacgc
     2581 cttccaactg aaggacggct ctttctacac ggcagaagtc aagaacaaca tagacttcaa
     2641 gaatccaatc cacgagtcct tctcgaagtc agggcccatg ttcacccaca gacgtgtcga
     2701 ggagactctc accaaggaga accttgccat agtggagtac cagcaggttt tcaacagcgc
     2761 cccaagagac atgtagGATT CGTCAGTAGG GTTGTAAAGG TTTTTCTTTT CCTGAGAAAA
     2821 CAACCTTTTG TTTTCTCAGG TTTTGCTTTT TGGCCTTTCC CTAGCTTTAA AAAAAAAAAA
     2881 GCAAAAATAT GAAGATGAAG ATGAAATATT TGGTGTGTCA AATAAAAAGC TTGTGTGCTT
     2941 AAGTTTGTGT TTTTTTCTTG GCTTGTTGTG TTATGAATTT GTGGCTTTTT CTAATATTAA
     3001 ATGAATGTAA GATCTCATTA TAATGAATAA ACAAATGTTT CTATAATCCA TTGTGAATGT
     3061 TTTGTTGGAT CTCTTCTGCA GCATATAACT ACTGTATGTG CTATGGTATG GACTATGGAA
     3121 TATGATTAAA GATAAGATGG GCTCATAGAG TAAAACGAGG CGAGGGACCT ATAAACCTCC
     3181 CTTCATCATG CTATTTCATG ATCTATTTTA TAAAATAAAG ATGTAGAAAA AAGTAAGCGT
     3241 AATAACCGCA AAACAAATGA TTTAAAACAT GGCACATAAT GAGGAGATTA AGTTCGGTTT
     3301 ACGTTTATTT TAGTACTAAT TGTAACGTGA GACTACGTAT CGGGAATCGC CTAATTAAAG
     3361 CATTAATGCG AACCTGATTA GATTCACCGA CCCTCCTATC GTGTCGACCT TTCTGTTTCT
     3421 TAGAATTTTT TGGTAGTCTA TGTACTAATA ATGTCAGCTT CGTATTTATT TCATAAGCAA
     3481 TTTGCATTTG CAATTTGTTT TTTACTTTTA TTTTTATTGT ATTGTGGAAT GTGGACTCGT
     3541 ACCAACATGA AGTTATATAC CACCAAAAAA ATTACAGTTA GTCAAAAGAT TCACGAGTGA
     3601 GAGCTACTTA TGATTGTCTT TTACGTATAT GTCTAATTGT CTATTTGCTC AATAATCTTT
     3661 GTACTTTCTT TTGTCGTTGA TAAAATCACA AAGTTCCAAA AGTAATCGAA TGATTTGCTT
     3721 TTAAGAAAAG AAGAGCTCAA TAATTCAACA TATATCTGTA CACAattacc gcctttgagt
     3781 gagctctaga tgccacctga cgtctaagaa gaattcgtaa tcatggtcat agctgtttcc
     3841 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3901 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3961 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4021 gagaggcggt ttgcgtattg gctagagcag cttgccaaca tggtggagca cgacactctc
     4081 gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt
     4141 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     4201 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
     4261 aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
     4321 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     4381 gataacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     4441 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     4501 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
     4561 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
     4621 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     4681 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     4741 tcccactatc cttcgcaaga ccttcctcta tataaggaag ttcatttcat ttggagagga
     4801 cacgctgaaa tcaccagtct ctctctacaa atctatctct ctcgagcttt cgcagatccc
     4861 ggggggcaat gagatatggg gattgaacaa gatggattgc acgcaggttc tccggccgct
     4921 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4981 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5041 ggtgccctga atgaactcca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5101 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5161 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5221 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5281 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5341 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5401 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgacac atggcgatgc ctgcttgccg
     5461 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5521 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5581 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5641 gccttctatc gccttcttga cgagttcttc tgaagtagat gccgaccgga tctgtcgatc
     5701 gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt
     5761 tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
     5821 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat
     5881 ccagatcccc cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     5941 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     6001 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     6061 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     6121 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     6181 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaccg cggtttcaaa
     6241 atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt
     6301 ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa
     6361 ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta
     6421 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     6481 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     6541 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
     6601 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     6661 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     6721 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     6781 actccatcga catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat
     6841 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca
     6901 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     6961 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     7021 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     7081 tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     7141 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     7201 tgttttagta cctagaatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7441 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7681 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7981 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8041 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     8101 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8161 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     8221 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     8281 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     8341 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
     8401 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
     8461 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
     8521 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
     8581 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
     8641 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
     8701 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
     8761 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
     8821 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
     8881 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
     8941 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
     9001 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
     9061 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
     9121 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
     9181 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
     9241 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
     9301 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
     9361 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
     9421 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
     9481 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
     9541 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
     9601 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
     9661 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
     9721 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
     9781 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
     9841 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc
     9901 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     9961 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
    10021 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
    10081 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
    10141 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
    10201 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
    10261 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
    10321 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
    10381 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
    10441 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
    10501 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
    10561 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
    10621 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
    10681 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
    10741 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
    10801 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
    10861 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
    10921 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    10981 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    11041 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    11101 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    11161 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    11221 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    11281 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    11341 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    11401 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    11461 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    11521 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    11581 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    11641 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    11701 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    11761 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    11821 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    11881 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    11941 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    12001 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    12061 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    12121 caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
    12181 ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct cttttctctt
    12241 aggtttaccc gccaatatat cctgtcaaac actgatagtt taattcccga tctagtaaca
    12301 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
    12361 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
    12421 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
    12481 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
    12541 cggggaaatt cgagctggtc accaattcac acgtgatggt gatggtgatg gctagcgttc
    12601 ttgtagccga aatctggaat gttggtccag cgctcgcgaa agacgtgcgc ggcgagcttc
    12661 ggcttgcggt cacgagtgaa cacgcccttc ttgtttcctt ggacgcgcat cacgccctga
    12721 gaggtcgcga agtccgcgaa gttccacgct tgctcaccca cgaagttctc aaactcatcg
    12781 aacacgacgt ggttcgcctg gtagtactcg acttgatatt cctcggtgaa catcactgga
    12841 tcaatgtcgt gaaagcccgc aacggtgtct gcgccgtact cagtgatcat gatcggcttt
    12901 cctgggcaac gcttgttcca cgcgtgaaat tcctggcgga gatggacttt ggccgcttcg
    12961 agatcaccgc catcgaagta ccatccgtta tagcgattga gcgcgatgac gtcaatcagt
    13021 tcggcgactt tgtccgtctc cggggtagcc atcacaaaca gcacgatcgt gaccggacgc
    13081 ttctgtgggt cgagttcctt ggtcagctcc accaacggct tgaagtactc gtacgcgccc
    13141 tcttcctcag tcgccgcctc gttggcgatg ctccacatca cgacgcttgg atggttcttg
    13201 tcacgagaca ccagttcacg gagaacgtct tgatggtgct caaacgtccg aatcttctcc
    13261 caggtactga cgcgctcgct gccttcgccg agtcccgtgg tggccatgaa gttgaggtgc
    13321 acgccaactg ccggagtctc gtcgatcacg accagaccct cgcgatccgc aagacgcatc
    13381 aactcttcag agtacggata gtgtgcggtc cggaagctgt tggcgccgat ccatttgagg
    13441 atattgaaat ccatcacatt gctcgcttcg ttaaagccac ggccgttgat aggagtgtcc
    13501 tcatgtttgc caaagccctt gaagtagaac ggtttgttgt tgatgaggaa cttgccgtcg
    13561 ttgacttcca cggtccgcac gccgaacggc tcttcataga catcgatggt cagtccgtcg
    13621 ttcaccagtt ccactttgat ctggtagaga tacgtgttca gtggttccca gaggatgaca
    13681 ttcggaatct ccacgttacc gctcaggccc tcggtgcttg cgaccacttt gccttcctca
    13741 tccacgaccg acactttcac ggtctcggct ttgccttgaa agtccaccgt ataggtcaca
    13801 gtcccggttg ggccattgaa gtcggtcaca accgagatgt cctcgacgta cgtaaacggg
    13861 gtcgtgtaga ttttcaccgg acggtgcagg cctgcatagt tgaagaagtc gaagttcggc
    13921 ttgttacgaa tgacttttcc gaggccctct tcgtggcgct cgctgtacag ccccaccggg
    13981 agggtgctat cgtcgaggat gttgtccacg gcgacggtga cgcgattcat gccatcacgc
    14041 agcgagttgt tgatttccgc ttcgaatggc aggaatccgc ccttgtgctc cacgaccagc
    14101 tcaccattga catagacaat tgctttgtga gttgcagagc cgaagcggag cacgatacgc
    14161 tgatccttca gataggccgg caccgtgaac tcacgttcgt accagacata tccgatatgg
    14221 ttgcggattt ccttggtcac gccaatgtca ttgtaactgc ttgggacggc catactaata
    14281 gtgtcggtca gcttgctttc gtaccacttc tcttccagtc ctttcccgta gtccagcttg
    14341 aagttccaga cgccattgag gtcgaagacg ccacgggtct cggtgttgat cgggtacaga
    14401 ctagttcgtc ggttctgtaa ctatcatcat catcatagac acacgaaata aagtaatcag
    14461 attatcagtt aaagctatgt aatatttaca ccataaccaa tcaattaaaa aatagatcag
    14521 tttaaagaaa gatcaaagct caaaaaaata aaaagagaaa agggtcctaa ccaagaaaat
    14581 gaaggagaaa aactagaaat ttaccctcag atctaccatg
//