LOCUS       pXYB177_dRibozyme_spli 14545 bp ds-DNA     circular     05-JAN-2024
DEFINITION  .
KEYWORDS    "creator:Xiaohan Yang" "marker:NeoR/KanR, KanR, Modified
            COR47-5'-UTR"
FEATURES             Location/Qualifiers
     promoter        complement(16..361)
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     738..754
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     primer          738..754
                     /label="oYL_ORNL048"
                     /note="sequence: gtaaaacgacggccagt"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(773..790)
                     /label="oYL_ORNL_047"
                     /note="sequence: ggctaatctggggacctg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1269..1614
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     misc_feature    1599..1623
                     /label="overlap1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1624..1730
                     /label="Modified COR47-5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1706..1735
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1706..3358
                     /label="gYL112"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1739..1763
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1739..1954
                     /label="1st half GFPuv"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1739..2811
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTTFKIESRIHGNLNGEKFELVGGGVGEEGRLEIEMKTKDKPLAFSPFLLTTCMGYGFYHFASFPKGIKNIYK*QYLPL*IKVIRHAPGS*SLNQ*IASV*KARPSNCGKGVNSRSVPSLRGNFEMALQRVW**ADGHGPNHAAKS*VNRSSVDMDAVHKLNVGRGRCILLIRYSRTSP*WELADEVMQHWSRWELICMRKYID*FWSTRFMLQRTEVTPTPGRRSMKTAASWRSTSVTLTSSTRSSVTSSALDMDSQVRVRSSRTRS*RVVPRWT*CCQCPGTSSPAPTLTPSN*RTALSTRQKSRTT*TSRIQSTSPSRSQGPCSPTDVSRRLSPRRTLP*WSTSRFSTAPQETC"
     misc_feature    1769..1793
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1828..1849
                     /label="oYL_ORNL138"
                     /note="sequence: tcgcctcgagattgagatgaag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1955..1974
                     /label="P1 Loop WT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1975..1980
                     /label="Internal guide sequence"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1981..2367
                     /label="dRIbozyme"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1985..2007)
                     /label="oYL_ORNL135"
                     /note="sequence: CTACCAGGTGCATGCCTGATAAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2368..2811
                     /label="2nd half GFPuv"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2376..2395
                     /label="oYL_ORNL140"
                     /note="sequence: tgcaacgaacggaggttaca"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2712..2736
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2734..2753)
                     /label="oYL_ORNL139"
                     /note="sequence: tccttggtgagagtctcctc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2812..3689
                     /label="HSPT878"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2837..2861
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3323..3358
                     /label="Gibson overhang"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3690..3710
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3741..3765
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3747..3767)
                     /label="oYL_ORNL029"
                     /note="sequence: caggaaacagctatgaccatg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3751..3767)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    3775..3791
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3799..3805)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3806..3823)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3824..3829)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3844..3865
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        4056..4733
                     /label="CaMV 35S promoter (enhanced)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
     misc_feature    4737..5663
                     /label="gBlocks for change to Kan"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4801..5598
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5638..5812
                     /label="CaMV poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: cauliflower mosaic virus polyadenylation signal"
     misc_feature    5890..5914
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             6339..7133
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aphA-3 note: confers resistance to kanamycin product: aminoglycoside phosphotransferase protein_id: transl_table: 1 translation: MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
     rep_origin      7220..7808
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    7994..8134
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     rep_origin      8478..8672
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(8738..9805)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 note: product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) protein_id: transl_table: 1 translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(10239..10868)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) transl_table: 1 translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     misc_feature    12168..12192
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     terminator      12214..12466
                     /label="NOS terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: nopaline synthase terminator and poly(A) signal"
     primer          12437..12463
                     /label="oYL072_oNB395 copy"
                     /note="sequence: GAAACTTTATTGCCAAATGTTTGAACG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(12498..12515)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     CDS             complement(12522..14339)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14340..14529
                     /label="cat1 intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: castor bean catalase intron, modified"
     CDS             complement(14340..14529)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             complement(14530..14544)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actCAAACAT TACTCATTCA CAAAACCATC TTAAAGCAAC TACACAAGTC TTGAAATTTT
     1681 CTCATATTTT CTATTTACTA TATAAACTTT TAATCAAATC AAGATTAAAG TTAATTAAat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatAAATAG CAATATTTAC CTTTGtagat
     1981 AAAAGTTATC AGGCATGCAC CTGGTAGCTA GTCTTTAAAC CAATAGATTG CATCGGTTTA
     2041 AAAGGCAAGA CCGTCAAATT GCGGGAAAGG GGTCAACAGC CGTTCAGTAC CAAGTCTCAG
     2101 GGGAAACTTT GAGATGGCCT TGCAAAGGGT ATGGTAATAA GCTGACGGAC ATGGTCCTAA
     2161 CCACGCAGCC AAGTCCTAAG TCAACAGATC TTCTGTTGAT ATGGATGCAG TTCACAAACT
     2221 AAATGTCGGT CGGGGAAGAT GTATTCTTCT CATAAGATAT AGTCGGACCT CTCCTTAATG
     2281 GGAGCTAGCG GATGAAGTGA TGCAACACTG GAGCCGCTGG GAACTAATTT GTATGCGAAA
     2341 GTATATTGAT TAGTTTTGGA GTACTCGctt catgctgcaa cgaacggagg ttacaccaac
     2401 accaggaagg agatctatga agacggcggc atcttggagg tcaacttccg ttacacttac
     2461 gagttcaaca agatcatcgg tgacgtcgag tgcattggac atggattccc aagtcagagt
     2521 ccgatcttca aggacacgat cgtgaagagt tgtcccacgg tggacctgat gttgccaatg
     2581 tccgggaaca tcatcgccag ctcctacgct tacgccttcc aactgaagga cggctctttc
     2641 tacacggcag aagtcaagaa caacatagac ttcaagaatc caatccacga gtccttctcg
     2701 aagtcagggc ccatgttcac ccacagacgt gtcgaggaga ctctcaccaa ggagaacctt
     2761 gccatagtgg agtaccagca ggttttcaac agcgccccaa gagacatgta gATATGAAGA
     2821 TGAAGATGAA ATATTTGGTG TGTCAAATAA AAAGCTTGTG TGCTTAAGTT TGTGTTTTTT
     2881 TCTTGGCTTG TTGTGTTATG AATTTGTGGC TTTTTCTAAT ATTAAATGAA TGTAAGATCT
     2941 CATTATAATG AATAAACAAA TGTTTCTATA ATCCATTGTG AATGTTTTGT TGGATCTCTT
     3001 CTGCAGCATA TAACTACTGT ATGTGCTATG GTATGGACTA TGGAATATGA TTAAAGATAA
     3061 GATGGGCTCA TAGAGTAAAA CGAGGCGAGG GACCTATAAA CCTCCCTTCA TCATGCTATT
     3121 TCATGATCTA TTTTATAAAA TAAAGATGTA GAAAAAAGTA AGCGTAATAA CCGCAAAACA
     3181 AATGATTTAA AACATGGCAC ATAATGAGGA GATTAAGTTC GGTTTACGTT TATTTTAGTA
     3241 CTAATTGTAA CGTGAGACTA CGTATCGGGA ATCGCCTAAT TAAAGCATTA ATGCGAACCT
     3301 GATTAGATTC ACCGACCCTC CTATCGTGTC GACCTTTCTG TTTCTTAGAA TTTTTTGGTA
     3361 GTCTATGTAC TAATAATGTC AGCTTCGTAT TTATTTCATA AGCAATTTGC ATTTGCAATT
     3421 TGTTTTTTAC TTTTATTTTT ATTGTATTGT GGAATGTGGA CTCGTACCAA CATGAAGTTA
     3481 TATACCACCA AAAAAATTAC AGTTAGTCAA AAGATTCACG AGTGAGAGCT ACTTATGATT
     3541 GTCTTTTACG TATATGTCTA ATTGTCTATT TGCTCAATAA TCTTTGTACT TTCTTTTGTC
     3601 GTTGATAAAA TCACAAAGTT CCAAAAGTAA TCGAATGATT TGCTTTTAAG AAAAGAAGAG
     3661 CTCAATAATT CAACATATAT CTGTACACAa ttaccgcctt tgagtgagct ctagatgcca
     3721 cctgacgtct aagaagaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3781 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3841 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3901 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3961 tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta ctccaagaat
     4021 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
     4081 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
     4141 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     4201 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     4261 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
     4321 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct
     4381 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
     4441 atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat
     4501 tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga
     4561 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     4621 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     4681 caagaccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tgaaatcacc
     4741 agtctctctc tacaaatcta tctctctcga gctttcgcag atcccggggg gcaatgagat
     4801 atggggattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     4861 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     4921 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4981 ctccaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     5041 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     5101 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5161 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5221 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5281 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     5341 gacggcgagg atctcgtcgt gacacatggc gatgcctgct tgccgaatat catggtggaa
     5401 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5461 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5521 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5581 cttgacgagt tcttctgaag tagatgccga ccggatctgt cgatcgacaa gctcgagttt
     5641 ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg
     5701 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
     5761 tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat
     5821 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     5881 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     5941 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     6001 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     6061 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     6121 taacgatgac agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat
     6181 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
     6241 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
     6301 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
     6361 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
     6421 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
     6481 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
     6541 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
     6601 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
     6661 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
     6721 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
     6781 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
     6841 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
     6901 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
     6961 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
     7021 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     7081 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
     7141 aatgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7861 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7921 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7981 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     8041 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     8101 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     8161 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     8221 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc
     8281 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     8341 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     8401 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     8461 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     8521 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     8581 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     8641 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc
     8701 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     8761 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     8821 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     8881 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     8941 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
     9001 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
     9061 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
     9121 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
     9181 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
     9241 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
     9301 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
     9361 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
     9421 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
     9481 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
     9541 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
     9601 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
     9661 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
     9721 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
     9781 gtaccgggcc ggatggtttg cgaccgtcac gccgattcct cgggcttggg ggttccagtg
     9841 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     9901 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     9961 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    10021 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    10081 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    10141 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    10201 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    10261 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    10321 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    10381 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    10441 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    10501 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    10561 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    10621 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    10681 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    10741 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    10801 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    10861 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    10921 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    10981 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    11041 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    11101 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    11161 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    11221 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    11281 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    11341 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    11401 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    11461 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    11521 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    11581 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    11641 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    11701 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    11761 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    11821 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    11881 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    11941 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    12001 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    12061 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    12121 cgccctttta aatatccgtt attctaataa acgctctttt ctcttaggtt tacccgccaa
    12181 tatatcctgt caaacactga tagtttaatt cccgatctag taacatagat gacaccgcgc
    12241 gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata
    12301 attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa
    12361 ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa
    12421 caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg aaattcgagc
    12481 tggtcaccaa ttcacacgtg atggtgatgg tgatggctag cgttcttgta gccgaaatct
    12541 ggaatgttgg tccagcgctc gcgaaagacg tgcgcggcga gcttcggctt gcggtcacga
    12601 gtgaacacgc ccttcttgtt tccttggacg cgcatcacgc cctgagaggt cgcgaagtcc
    12661 gcgaagttcc acgcttgctc acccacgaag ttctcaaact catcgaacac gacgtggttc
    12721 gcctggtagt actcgacttg atattcctcg gtgaacatca ctggatcaat gtcgtgaaag
    12781 cccgcaacgg tgtctgcgcc gtactcagtg atcatgatcg gctttcctgg gcaacgcttg
    12841 ttccacgcgt gaaattcctg gcggagatgg actttggccg cttcgagatc accgccatcg
    12901 aagtaccatc cgttatagcg attgagcgcg atgacgtcaa tcagttcggc gactttgtcc
    12961 gtctccgggg tagccatcac aaacagcacg atcgtgaccg gacgcttctg tgggtcgagt
    13021 tccttggtca gctccaccaa cggcttgaag tactcgtacg cgccctcttc ctcagtcgcc
    13081 gcctcgttgg cgatgctcca catcacgacg cttggatggt tcttgtcacg agacaccagt
    13141 tcacggagaa cgtcttgatg gtgctcaaac gtccgaatct tctcccaggt actgacgcgc
    13201 tcgctgcctt cgccgagtcc cgtggtggcc atgaagttga ggtgcacgcc aactgccgga
    13261 gtctcgtcga tcacgaccag accctcgcga tccgcaagac gcatcaactc ttcagagtac
    13321 ggatagtgtg cggtccggaa gctgttggcg ccgatccatt tgaggatatt gaaatccatc
    13381 acattgctcg cttcgttaaa gccacggccg ttgataggag tgtcctcatg tttgccaaag
    13441 cccttgaagt agaacggttt gttgttgatg aggaacttgc cgtcgttgac ttccacggtc
    13501 cgcacgccga acggctcttc atagacatcg atggtcagtc cgtcgttcac cagttccact
    13561 ttgatctggt agagatacgt gttcagtggt tcccagagga tgacattcgg aatctccacg
    13621 ttaccgctca ggccctcggt gcttgcgacc actttgcctt cctcatccac gaccgacact
    13681 ttcacggtct cggctttgcc ttgaaagtcc accgtatagg tcacagtccc ggttgggcca
    13741 ttgaagtcgg tcacaaccga gatgtcctcg acgtacgtaa acggggtcgt gtagattttc
    13801 accggacggt gcaggcctgc atagttgaag aagtcgaagt tcggcttgtt acgaatgact
    13861 tttccgaggc cctcttcgtg gcgctcgctg tacagcccca ccgggagggt gctatcgtcg
    13921 aggatgttgt ccacggcgac ggtgacgcga ttcatgccat cacgcagcga gttgttgatt
    13981 tccgcttcga atggcaggaa tccgcccttg tgctccacga ccagctcacc attgacatag
    14041 acaattgctt tgtgagttgc agagccgaag cggagcacga tacgctgatc cttcagatag
    14101 gccggcaccg tgaactcacg ttcgtaccag acatatccga tatggttgcg gatttccttg
    14161 gtcacgccaa tgtcattgta actgcttggg acggccatac taatagtgtc ggtcagcttg
    14221 ctttcgtacc acttctcttc cagtcctttc ccgtagtcca gcttgaagtt ccagacgcca
    14281 ttgaggtcga agacgccacg ggtctcggtg ttgatcgggt acagactagt tcgtcggttc
    14341 tgtaactatc atcatcatca tagacacacg aaataaagta atcagattat cagttaaagc
    14401 tatgtaatat ttacaccata accaatcaat taaaaaatag atcagtttaa agaaagatca
    14461 aagctcaaaa aaataaaaag agaaaagggt cctaaccaag aaaatgaagg agaaaaacta
    14521 gaaatttacc ctcagatcta ccatg
//