LOCUS       pKG2039_pPV1-CAG        3503 bp    DNA     circular SYN 05-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3503
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        1..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(229..248)
                     /label=oSEQ065 CMV_rev
     promoter        363..640
                     /label=chicken beta-actin promoter
     intron          641..1657
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1672..1691
                     /label=OSEQ085 CagPromoter_fwd_SEQ
     primer_bind     1686..1725
                     /label=oEP133
     primer_bind     1705..1725
                     /label=oEP136
     primer_bind     1706..1736
                     /label=AmpR1_fwd
     primer_bind     1706..1724
                     /label=pPV1_bb_fwd
     primer_bind     1710..1725
                     /label=oEP028
     primer_bind     1710..1725
                     /label=oEP128
     primer_bind     1710..1725
                     /label=oEP034
     promoter        1750..1854
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1750..1769)
                     /label=oEP062
     primer_bind     complement(1787..1808)
                     /label=oSEQ095 oKL157
     CDS             join(1855..1923,1924..2715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1855..1879
                     /label=oSEQ083 Amp-fwd
     primer_bind     complement(2073..2092)
                     /label=oSEQ066 Amp_rev
     primer_bind     complement(2316..2335)
                     /label=oEP074
     primer_bind     2316..2335
                     /label=oEP075
     primer_bind     2553..2591
                     /label=Amp2-ori_fwd
     primer_bind     complement(2555..2572)
                     /label=AmpR1_rev
     rep_origin      2886..3474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2918..2937
                     /label=oEP033
     primer_bind     complement(2918..2937)
                     /label=oEP035
     primer_bind     3238..3258
                     /label=oEP024
     primer_bind     complement(3238..3258)
                     /label=oEP025
     primer_bind     3375..3394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3375..3394
                     /label=oSEQ067 pBR322-ori_fwd
     primer_bind     complement(3470..3498)
                     /label=Amp2-ori_rev
     primer_bind     complement(join(3480..3503,1..15))
                     /label=oEP134
     primer_bind     complement(3480..3498)
                     /label=pPV1_bb_rev
     primer_bind     3480..3498
                     /label=oEP139
     primer_bind     complement(3480..3498)
                     /label=oEP140
     primer_bind     complement(3480..3494)
                     /label=oEP050
     promoter        3500..3501
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
      421 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
      481 ggggcgcgcg ccrggsgggg sggggsgggg sgrggggsgg ggsggggcga ggcggagagg
      541 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
      601 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
      661 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
      721 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
      781 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
      841 gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
      901 cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
      961 ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     1021 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg
     1081 gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga
     1141 gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc
     1201 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccggggr
     1261 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
     1321 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
     1381 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
     1441 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
     1501 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
     1561 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     1621 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     1681 tgtgctgtct catcattttg gcaaaaatga gagacctaga cgtcaggtgg cacttttcgg
     1741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     2101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtatcgcggt
     2581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3481 agcaacgggt ctcatactac tag
//
