LOCUS       pKG0618_pPV1-CMV        2351 bp    DNA     circular SYN 21-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2351
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     complement(8..36)
                     /label=Amp2-ori_rev
     primer_bind     13..36
                     /label=oEP054
     primer_bind     13..36
                     /label=oEP056
     primer_bind     13..36
                     /label=oEP091
     primer_bind     13..36
                     /label=oEP092
     primer_bind     17..59
                     /label=pPV1-CMV_fwd
     primer_bind     17..54
                     /label=oEP143
     primer_bind     complement(18..36)
                     /label=oEP140
     primer_bind     complement(18..36)
                     /label=oEP134
     primer_bind     18..36
                     /label=oEP139
     primer_bind     18..32
                     /label=oEP048
     primer_bind     complement(18..32)
                     /label=oEP050
     enhancer        37..340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(208..227)
                     /label=oSEQ065 CMV_rev
     promoter        341..549
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     495..515
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     498..518
                     /label=oSEQ030 CMV_fwd
     primer_bind     complement(513..532)
                     /label=oSEQ042 SFCp_rev
     primer_bind     complement(526..564)
                     /label=pPV1-CMV_rev
     primer_bind     complement(530..614)
                     /label=oEP144
     primer_bind     complement(530..549)
                     /label=oEP138
     primer_bind     545..562
                     /label=pPV1-EF1a-bb_fwd
     primer_bind     complement(550..595)
                     /label=CMV-UTR-short-rev
     primer_bind     550..594
                     /label=CMV-UTR-short-fwd
     primer_bind     complement(576..614)
                     /label=pPV1-EF1a_rev
     primer_bind     594..614
                     /label=oEP137
     primer_bind     595..625
                     /label=AmpR1_fwd
     primer_bind     complement(595..618)
                     /label=oEP055
     primer_bind     595..614
                     /label=oEP128
     primer_bind     595..614
                     /label=oEP133
     primer_bind     595..614
                     /label=oEP136
     primer_bind     595..613
                     /label=pPV1_bb_fwd
     primer_bind     complement(595..611)
                     /label=oEP127
     primer_bind     complement(599..614)
                     /label=oEP049
     primer_bind     complement(599..614)
                     /label=oEP026
     primer_bind     599..614
                     /label=oEP028
     primer_bind     complement(599..614)
                     /label=oEP045
     primer_bind     complement(599..614)
                     /label=oEP031
     primer_bind     599..614
                     /label=oEP034
     primer_bind     complement(599..614)
                     /label=oEP118
     primer_bind     complement(599..613)
                     /label=oEP110
     promoter        639..743
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(639..658)
                     /label=oEP062
     CDS             join(744..812,813..1604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     744..768
                     /label=oSEQ083 Amp-fwd
     primer_bind     complement(962..981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(962..981)
                     /label=oSEQ066 Amp_rev
     primer_bind     complement(1205..1224)
                     /label=oEP074
     primer_bind     1205..1224
                     /label=oEP075
     primer_bind     1442..1480
                     /label=Amp2-ori_fwd
     primer_bind     complement(1444..1461)
                     /label=AmpR1_rev
     rep_origin      join(1775..2351,1..12)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1807..1826
                     /label=oEP033
     primer_bind     complement(1807..1826)
                     /label=oEP035
     primer_bind     2127..2147
                     /label=oEP024
     primer_bind     complement(2127..2147)
                     /label=oEP025
     primer_bind     2264..2283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2264..2283
                     /label=oSEQ067 pBR322-ori_fwd
ORIGIN
        1 agcctatgga aaaacgccag caacgggtct catactcgtt acataactta cggtaaatgg
       61 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      121 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      181 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      241 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      301 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      361 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      421 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      481 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      541 agctgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgtaatgag
      601 agacctagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      661 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      721 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      781 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      841 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      901 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      961 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     1021 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1081 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1141 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1201 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1261 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1321 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1381 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1441 ctggagccgg tgagcgtggg tatcgcggta tcattgcagc actggggcca gatggtaagc
     1501 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1561 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1621 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1681 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1741 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1801 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1861 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1921 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1981 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2041 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2101 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2161 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2221 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2281 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2341 caggggggcg g
//
