LOCUS       pKG1179_pPV1-EFS        2006 bp    DNA     circular SYN 01-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      49..637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     538..557
                     /label=oSEQ067 pBR322-ori_fwd
     primer_bind     638..678
                     /label=oKL204
     primer_bind     638..655
                     /label=oKL179
     primer_bind     complement(640..657)
                     /label=pPV-bb-rev-v2
     primer_bind     complement(643..661)
                     /label=pPV1_bb_rev
     promoter        662..873
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(704..721)
                     /label=oSEQ045 phage-dest-rev-2
     primer_bind     complement(855..899)
                     /label=oKL205
     primer_bind     875..893
                     /label=pPV1_bb_fwd
     primer_bind     879..899
                     /label=pPV-bb-fwd-v2
     promoter        919..1023
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(956..977)
                     /label=oKL157
     CDS             join(1024..1092,1093..1884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1242..1261)
                     /label=oSEQ066 Amp_rev
     primer_bind     complement(1438..1455)
                     /label=oKL048
     primer_bind     complement(1445..1462)
                     /label=oKL070
ORIGIN
        1 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
       61 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      181 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg ggtctcatac
      661 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      721 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      781 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      841 ctttttcgca acgggtttgc cgccagaaca cagtaatgag agacctagac gtcaggtggc
      901 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      961 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1021 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1081 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1141 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1201 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1261 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1321 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1381 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1441 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1501 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1561 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1621 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1681 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1741 tatcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1801 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1861 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1921 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1981 atgaccaaaa tcccttaacg tgagtt
//
