LOCUS       pKG0743_pPV1-ZF4        1956 bp    DNA     circular SYN 21-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1956
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        41..145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(146..214,215..1006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(364..383)
                     /label=oSEQ066 Amp_rev
     primer_bind     844..882
                     /label=Amp2-ori_fwd
     primer_bind     complement(846..863)
                     /label=AmpR1_rev
     rep_origin      1177..1765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1666..1685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1666..1685
                     /label=oSEQ067 pBR322-ori_fwd
     primer_bind     1760..1807
                     /label=ZF43_6x-C-fwd
     primer_bind     complement(1761..1789)
                     /label=Amp2-ori_rev
     primer_bind     1766..1810
                     /label=oEP054
     primer_bind     complement(1771..1789)
                     /label=pPV1_bb_rev
     misc_feature    1790..1952
                     /label=ZF43_6x_C-promoter
     protein_bind    1808..1816
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1823..1831
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1838..1846
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1853..1861
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1868..1876
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1883..1891
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     primer_bind     complement(1932..1952)
                     /label=ZF43_6x_C-rev
     primer_bind     complement(join(1933..1956,1..20))
                     /label=oEP055
     primer_bind     join(1952..1956,1..27)
                     /label=AmpR1_fwd
     primer_bind     join(1953..1956,1..15)
                     /label=pPV1_bb_fwd
ORIGIN
        1 agagacctag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
       61 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      121 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      181 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      241 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      301 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      361 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
      421 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
      481 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
      541 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
      601 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
      661 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
      721 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
      781 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
      841 atctggagcc ggtgagcgtg ggtatcgcgg tatcattgca gcactggggc cagatggtaa
      901 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
      961 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1021 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1081 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1141 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1201 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1261 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1321 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1381 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1441 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1501 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1561 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1621 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1681 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1741 gtcagggggg cggagcctat ggaaaaacgc cagcaacggg tctcatacta gatctgaatt
     1801 acgatcggag tgaggataat tagagtgagg acgatcggag tgaggaaaat tagagtgagg
     1861 acgatcggag tgaggataat tagagtgagg acgatccctc tagagggtat ataatggggg
     1921 ccactagtct actaccagag ctcatcgcta gcaatg
//
