LOCUS       pKG3657_pPV1-ZF1        2250 bp    DNA     circular SYN 13-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2250
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      49..637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     538..557
                     /label=oSEQ067 pBR322-ori_fwd
     primer_bind     638..655
                     /label=oKL179
     primer_bind     640..686
                     /label=oKL270
     primer_bind     complement(640..657)
                     /label=pPV-bb-rev-v2
     primer_bind     complement(643..661)
                     /label=pPV1_bb_rev
     misc_feature    663..1047
                     /label=8X ZF10 BD
                     /note="locus_tag: 8X ZF10 BD label: 8X ZF10 BD 
                     ApEinfo_revcolor: #0080c0 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    702..721
                     /label=ZF10 BS
     misc_feature    740..759
                     /label=ZF10 BS
     misc_feature    778..797
                     /label=ZF10 BS
     misc_feature    816..835
                     /label=ZF10 BS
     misc_feature    875..894
                     /label=ZF10 BS
     misc_feature    913..932
                     /label=ZF10 BS
     misc_feature    951..970
                     /label=ZF10 BS
     misc_feature    989..1008
                     /label=ZF10 BS
     misc_feature    1054..1078
                     /label=ybTATA
                     /note="locus_tag: ybTATA label: ybTATA ApEinfo_revcolor: 
                     #800040 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} 
                     {} 0}width 5 offset 0"
     primer_bind     complement(1101..1143)
                     /label=oKL327
     primer_bind     1119..1137
                     /label=pPV1_bb_fwd
     primer_bind     1123..1143
                     /label=pPV-bb-fwd-v2
     primer_bind     complement(1124..1143)
                     /label=oKL247
     promoter        1163..1267
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1200..1221)
                     /label=oKL157
     primer_bind     complement(1200..1221)
                     /label=oSEQ095 oKL157
     CDS             join(1268..1336,1337..2128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1268..1292
                     /label=oSEQ083 Amp-fwd
     primer_bind     complement(1486..1505)
                     /label=oSEQ066 Amp_rev
     primer_bind     complement(1682..1699)
                     /label=oKL048
     primer_bind     1682..1699
                     /label=oKL191
     primer_bind     complement(1689..1706)
                     /label=oKL070
ORIGIN
        1 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
       61 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      181 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg ggtctcatac
      661 tgacgattga gtaacactgt atctagtcta cctacagggc tcggcgtagc cgatgtcgcg
      721 cggatagcgt caacctcttc ggcgtagccg atgtcgcgca atcagttctc ccgaggtcgg
      781 cgtagccgat gtcgcgcaga gccgtgcttc caatacggcg tagccgatgt cgcgccgggt
      841 ttcgtaacaa tcgcatgagg attcgcaacg ccttcggcgt agccgatgtc gcgctcccgt
      901 ctcagtaaag gtcggcgtag ccgatgtcgc gcaatcggac tgccttcgta cggcgtagcc
      961 gatgtcgcgc gtatcagtcg cctcggaacg gcgtagccga tgtcgcgcat tcgtaagagg
     1021 ctcactctcc cttacacgga gtggatagga tcctctagag ggtatataat gggggccact
     1081 agtctactac cagagctcat cgctagcgct accggatcaa tgagagacct agacgtcagg
     1141 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1201 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1261 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1321 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1381 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1441 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1501 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1561 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1621 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1681 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1741 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1801 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1861 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1921 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1981 tgggtatcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2041 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2101 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2161 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2221 tctcatgacc aaaatccctt aacgtgagtt
//
