LOCUS       pKG1313_pPV3-WPR        2388 bp    DNA     circular SYN 15-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..43)
                     /label=Amp2-ori_rev
     primer_bind     24..81
                     /label=geWPRE_fwd
     primer_bind     complement(26..43)
                     /label=pPV1_bb_rev_v2
     primer_bind     complement(35..53)
                     /label=ppv3a-mirTS-Rev
     primer_bind     48..77
                     /label=GG_WPRE_fwd
     misc_feature    54..642
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
     primer_bind     complement(220..239)
                     /label=oSEQ034 WPRE_rev
     primer_bind     477..497
                     /label=oSEQ048 phage-dest-fwd-2
     primer_bind     complement(625..666)
                     /label=geWPRE_rev
     primer_bind     complement(625..666)
                     /label=geWPRE_v2_rev
     primer_bind     complement(633..642)
                     /label=GG_WPRE_rev
     primer_bind     complement(643..666)
                     /label=geSV40_rev
     primer_bind     647..673
                     /label=AmpR1_fwd
     primer_bind     647..667
                     /label=pPV1_bb_fwd_v2
     primer_bind     647..666
                     /label=ppv3a-mirTS-Fwd
     promoter        687..791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(792..860,861..1652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     792..816
                     /label=oSEQ083 Amp-fwd
     primer_bind     complement(1010..1029)
                     /label=oSEQ066 Amp_rev
     primer_bind     1490..1528
                     /label=Amp2-ori_fwd
     primer_bind     complement(1492..1509)
                     /label=AmpR1_rev
     rep_origin      join(1823..2388,1..23)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2312..2331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caggggggcg gagcctatgg aaaaacgcca gcaacgggtc tcacaacgcc gataatcaac
       61 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
      121 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
      181 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
      241 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
      301 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
      361 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
      421 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
      481 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
      541 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
      601 gccctcagac gagtcggatc tccctttggg ccgcctcccc gccgctagag acctagacgt
      661 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      721 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      781 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      841 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      901 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      961 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1021 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1081 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1141 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1201 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1261 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1321 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1381 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1441 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1501 agcgtgggta tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1561 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1621 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1681 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1741 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1801 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1861 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1921 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1981 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2041 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2101 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2161 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2221 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2281 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2341 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgt
//
