LOCUS       Exported                8730 bp ds-DNA     circular SYN 08-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 8, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Storage Type: Constructed
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    5..1269
                     /label=hENO2
                     /note="color: #a6acb3"
                     /note="color: #a6acb3"
     misc_feature    1640..5389
                     /label=NF186-HA
                     /label=NF-186, uniprot P97685 (NFASC_RAT)
                     /note="color: #a6acb3"
                     /note="color: #a6acb3"
     misc_feature    3677..3679
                     /label=K680TAG
                     /note="color: #a6acb3"
                     /note="color: #ff0000"
     misc_feature    5360..5386
                     /label=HA tag
                     /note="color: #a6acb3"
                     /note="color: #a6acb3"
     promoter        complement(5639..6094)
                     /label=f1Ori
                     /note="color: #ffffff; direction: LEFT"
                     /note="color: #ffffff; direction: LEFT"
     promoter        6619..7413
                     /label=Kan/NeoR
                     /note="color: #ffffff"
                     /note="color: #ffffff"
ORIGIN
        1 taattgtatg cagctggacc taggagagaa gcaggagagg aagatccagc acaaaaaatc
       61 cgaagctaaa aacaggacac agagatgggg gaagaaaaga gggcagagtg aggcaaaaag
      121 agactgaaga gatgagggtg gccgccaggc actttagata ggggagaggc tttatttacc
      181 tctgtttgtt tttttttttt tttttttttt tttgcgaggt agtcttgctt agtctccagg
      241 ctggagtgca gtggcacaat ctcagctcac tgcaacttcc acctcctggg ttcaagcaat
      301 tctcctgcct cagcctcccg agtagctggg actacaggcg catgcaaccg cgcctggcta
      361 atttttgtat ttttagtaga aacggggttt caccacgtta gccaggatgg tctggatctc
      421 ctgacctcgt gatctgcccg cctccgcctt ccaaagtgct gggattacag gggtgagcca
      481 cagcgcctgg tccctattta cttctgtctt ctacctccag gagatcaaag acgctggcct
      541 tcagacctga tcagactccc aggggcagcc accacatgta tgacagagaa cagaggatgc
      601 ctgtttttgc cccaaagctg gaaattcatc acaacctgag gcccaggatc tgctctgtgc
      661 cggtcctctg ggcagtgtgg ggtgcagaat ggggtgccta ggcctgagcg ttgcctggag
      721 cctaggccgg gggccgccct cgggcaggcg tgggtgagag ccaagaccgc gtgggccgcg
      781 gggtgctggt aggagtggtt ggagagactt gcgaaggcgg ctggggtgtt cggatttcca
      841 ataaagaaac agagtgatgc tcctgtgtct gaccgggttt gtgagacatt gaggctgtct
      901 tgggcttcac tggcagtgtg ggccttcgta cccgggctac aggggtgcgg ctctgcctgt
      961 tactgtcgag tgggtcgggc cgtgggtatg agcgcttgtg tgcgctgggg ccaggtcgtg
     1021 ggtgccccca cccttccccc atcctcctcc cttccccact ccaccctcgt cggtccccca
     1081 cccgcgctcg tacgtgcgcc tccgccggca gctcctgact catcgggggc tccgggtcac
     1141 atgcgcccgc gcggccctat aggcgcctcc tccgcccgcc gcccgggagc cgcagccgcc
     1201 gccgccactg ccactcccgc tctctcagcg ccgccgtcgc caccgccacc gccaccgcca
     1261 ctaccaccgg ctagcgctac cggactcaga tctcgagctc aagcttgata tcgaattccg
     1321 ggcggcggct ggcggcgaga gctgctgcag gggacgcggg cgaagtggcg gcgccgggag
     1381 cagacatctg ggactgcgcc cagggctgga gctggagctc ctggaggtaa ggttgactga
     1441 cttatgtgcg atttgggagc ctggagtttt cttgccctct gccgctgccg tcaaccggag
     1501 cagagcctcc gctggtgttg cgaaaagcag gccgaatgag gcagagaagc tgagcactgt
     1561 ctggaaagcc cacgaggaac ggcgcaaggc atcgagagcc cgtgggctgg gcagtgcgga
     1621 gcagggcctg gcgctgagga tggccaggca gcaggcgcca ccctgggtcc acgtagccct
     1681 catcctcttc ctcctcagcc tcggaggggc cattgagatt ccgatggatc caagcattca
     1741 gaatgagctg acccaacccc caacgatcac caagcagtcg gtgaaggacc acatcgtgga
     1801 cccccgagat aacatcctga ttgaatgtga agctaaaggg aaccccgccc ccagtttcca
     1861 ctggactcgc aacagcaggt tcttcaacat tgccaaggac ccacgggtgt ccatgaggag
     1921 gaggtctggg accttggtga tcgacttccg cagtggtggg cggcctgagg agtacgaagg
     1981 ggagtaccag tgctttgccc ggaacaaatt cggcacagct cttagcaacc gcatccgcct
     2041 gcaggtgtcc aaatctcccc tgtggcccaa ggaaaaccta gaccccgtcg tggttcaaga
     2101 gggtgccccc ttaaccctgc agtgcaaccc cccacctggc ctcccatccc ccgtcatctt
     2161 ctggatgagc agctccatgg agcccatcac ccaggacaag cgtgtctccc agggtcacaa
     2221 cggggacctg tacttctcca acgtcatgct gcaggacatg cagaccgact acagctgcaa
     2281 tgcacgcttc cacttcaccc acaccattca gcagaagaat cccttcaccc tcaaagtcct
     2341 caccacccga ggagttgcgg aaagaacacc tagcttcatg tatccccagg gcacgtcaag
     2401 cagtcagatg gtactgcgcg gcatggacct gctgctggag tgcattgcct ctggggtccc
     2461 aacaccagat attgcatggt acaagaaagg tggggacctc ccatctgaca aggccaagtt
     2521 cgagaatttt aacaaggctc tgcgcatcac caatgtctct gaagaggact ctggggagta
     2581 tttctgcctg gcctccaaca agatgggcag catccggcac acgatctcgg tgagagtaaa
     2641 ggctgcccca tactggctgg atgagcccaa gaacttgatc ctggctcctg gtgaagatgg
     2701 gaggctggtg tgtcgagcca atgggaaccc gaagccgacc gtccagtggc tggtgaatgg
     2761 agaccctttg caatcggcac cacccaaccc caaccgtgag gtggccggag acactatcat
     2821 cttccgggac actcagatca gcagcagggc agtgtaccag tgtaacacat ccaacgaaca
     2881 tggctacctg ctggccaatg ccttcgtcag cgtattagat gtaccccctc ggatgctgtc
     2941 tccccggaac cagctcatca gggtgatcct ttacaaccgg acgcgactgg actgtccgtt
     3001 ctttgggtct cccattccaa cactccgatg gtttaagaat gggcaaggaa gcaacctgga
     3061 tggtggtaac taccacgtct acgaaaatgg cagcctggaa atcaagatga ttcgcaaaga
     3121 ggaccaaggc atctacacct gtgtggccac caacatcctg ggaaaagctg aaaatcaagt
     3181 ccgcctggag gtcaaagacc ccaccaggat ctacaggatg cctgaagacc aggtggccaa
     3241 gaggggcacc acagtgcagc tggagtgccg tgtgaagcat gacccctcct tgaaactcac
     3301 agtctcctgg ctgaaggatg acgagccact ctacattgga aacaggatga aaaaggaaga
     3361 tgactccctg accatcttcg gagtggcaga gcgggaccaa ggcagttaca cgtgcatggc
     3421 cagcaccgag ctggaccagg acctggcaaa ggcctacctc actgttctag ctgatcaggc
     3481 cactccgact aaccgtttgg ctgccctgcc caaagggcgg ccagaccgac ccagggacct
     3541 ggagctcact gacctggcgg aaaggagtgt gaggctgacc tggatcccgg gggatgataa
     3601 caacagtccc atcacagact acgttgtcca gtttgaagag gaccagttcc agccaggagt
     3661 ctggcatgac cactcctagt tcccaggcag tgtcaactca gccgtcctcc atctgtcccc
     3721 gtatgtcaac tatcagttcc gagtcatcgc tgtcaacgag gttgggagca gccaccccag
     3781 ccttccatcc gagcggtacc gaacaagcgg ggcaccccct gaatccaacc ccagtgatgt
     3841 gaagggcgaa gggacacgaa aaaacaatat ggagatcaca tggacgccta tgaatgctac
     3901 ctccgccttt ggccccaacc tgcgctacat tgtcaagtgg cgacggagag aaacccgaga
     3961 gacttggaac aatgtcaccg tgtggggctc tcgctatgtg gtggggcaga cccctgtcta
     4021 cgtaccctat gagatccgag tccaggctga aaatgacttt gggaaaggcc cggagcctga
     4081 aaccgtcatt gggtactcgg gggaagattt acccagtgcc cccaggcgtt tcagagtccg
     4141 acagcccaac ctggagacca tcaacctgga atgggatcac ccagagcacc ccaacgggat
     4201 cctgattgga tacacgctca gatacgtgcc ctttaatgga accaaactgg gaaagcagat
     4261 ggtggaaaac ttctctccca atcagaccaa gttctcggtg cagagagcag accccgtgtc
     4321 ccgttaccgc ttctccctca gtgccaggac gcaggtgggc tctggagaag cagccacgga
     4381 ggagtcccca acacctccaa atgaagctac tccaactgca gctcctccca cattgccccc
     4441 gactaccgtg ggtaccacag gccttgtgag cagtactgat gctactgccc ttgctgccac
     4501 cagtgaagcc acaacagttc ccatcatccc aaccgtcgta cctaccaccg tcgccaccac
     4561 cattgccaca actactacaa ccactgccgc cgccaccacc actaccacca cggagagtcc
     4621 tcccactacc accactggga ctaagattca cgaaaccgcc cccgacgagc agtccatttg
     4681 gaacgtcacg gtgctcccca acagtaaatg ggccaacatc acctggaagc acaatttcag
     4741 gcctggaact gactttgtgg ttgagtacat cgacagcaac catacgaaaa aaactgtccc
     4801 tgttaaggcc caagcccagc ccatacagct gacagacctc tttcccggga tgacgtacac
     4861 gttgcgggtg tattcccggg acaacgaggg catcagcagt accgtcatca cctttatgac
     4921 cagtacagct tacaccaaca accagactga catcgccacc cagggctggt tcatcgggct
     4981 catgtgtgcc attgcccttc tggtgctgat cctgcttatc gtctgcttca tcaagaggag
     5041 tcgtggcggc aagtacccag tgcgagaaaa gaaggatgtc cccttgggcc ctgaagaccc
     5101 caaagaagaa gatggttcat ttgactacag tgacgaggac aacaagcccc tgcagggcag
     5161 ccagacatct ctggacggca ccatcaagca gcaggagagt gacgacagcc tagtggacta
     5221 cggcgagggt ggcgagggcc agttcaacga agatggctcc tttattggcc agtacactgt
     5281 cagaaaggac aaggaggaga ccgagggcaa tgagagctca gaggccacat ctccagtcaa
     5341 tgccatctat tccctggcct acccatacga cgtcccagac tacgcttgag cggccgcgac
     5401 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     5461 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     5521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5581 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa
     5641 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     5701 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     5761 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     5821 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     5881 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     5941 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     6001 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     6061 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     6121 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6181 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     6241 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6301 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6361 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6421 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6481 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6541 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     6601 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     6661 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     6721 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6781 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     6841 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     6901 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     6961 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     7021 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     7081 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     7141 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     7201 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     7261 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     7321 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7381 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     7441 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     7501 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7561 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     7621 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     7681 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     7741 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     7801 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     7861 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7921 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7981 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8041 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8101 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8161 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8221 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8281 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8341 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8401 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8461 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8521 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8581 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8641 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8701 ataaccgtat taccgccatg cattagttat
//
