LOCUS       pHS1376                 3131 bp ds-DNA     circular     09-MAY-2025
DEFINITION  .
COMMENT     ^^
FEATURES             Location/Qualifiers
     misc_feature    751..755
                     /label="RBS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    762..764
                     /label="Start codon"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        780..1028
                     /label="Human U6 Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1029..1051
                     /label="Golden gate site for spacer cloning"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          1031..1036
                     /label="pUC19"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1044..1049
                     /label="BbsI"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1052..1256
                     /label="OrufIscB omegaRNA scaffold"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1053..1118
                     /label="guide adaptor hairpin"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1197..1249
                     /label="terminal hairpin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     source          complement(1676..1675)
                     /label="pUC19"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     -35_signal      1806..1811
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     -10_signal      1827..1832
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     sig_peptide     1876..1944
                     /label="bla"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1876..2736
                     /label="bla"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      2907..364
                     /label="pMB1 origin of replication"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     -35_signal      2907..2912
                     /label="RNAII promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     -10_signal      2928..2933
                     /label="RNAII promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_RNA        2943..364
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_RNA        complement(2946..3053)
                     /label="RNAI transcript"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     -10_signal      complement(3062..3067)
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     -35_signal      complement(3084..3089)
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
       61 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      121 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      181 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      241 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      301 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      361 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      421 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      481 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      541 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      601 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      661 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      721 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccag
      781 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      841 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      901 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      961 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1021 gaaacaccAT GTCTTCCTGG GACGAAGACA AGTCAATAAC CCATGACTGA AGTCATGGGC
     1081 TTGCAGATGC AGGTCCTGAT GGAAGAAAGG GTTACTGAGC AGAGCAGTGA CATGTCATTC
     1141 GCCGCGGGGT GATTCCAAGC TCCGCGCTCC GGCTAGACAT GCCCATGCTA TGGAAACTTT
     1201 AACGGTATGT GCGGTTTTCC GCTCATACCG GCTTACAACA AATAAGGAGT TATTAGTTTT
     1261 TTggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     1321 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     1381 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     1441 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     1501 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     1561 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     1621 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     1681 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     1741 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1801 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1861 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1921 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1981 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2041 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2101 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     2161 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2221 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2281 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2341 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     2401 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2461 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     2521 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     2581 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2641 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2701 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2761 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2821 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2881 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2941 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3001 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3061 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3121 ctgctaatcc t
//