LOCUS       pHS1505                 7344 bp ds-DNA     circular     09-MAY-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(850..948)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        1217..1804
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1743..1761
                     /label="CMV For"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1754..1774
                     /label="CMV forward primer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        1848..1864
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1848..1867
                     /label="T7 primer"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1867..1896
                     /label="HindIII GH"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1908..3878
                     /label="Translation 1908-3878"
                     /translation="MGSPKKKRKVGSRYVYVLDVDGKPLMPTCRFGKVRRMLKSGQAKAVDTLPFTIQLTYKPRTRILQPVTLGQDPGRTNIGMAAVRFDGKELGRFHCITRNKEIPKLMADRMAARKASRRGERLARKRLARKLHTTAKHLNGRILPGCSEPIAVKDIINTESRFNNRILTKCKVCGKNTPLRRNVRELLLENIVRFLPLESELKETLKRTILEGQQGNINKLFRKLKFNQKDWPGKNLTDIAKNKLPGRLPFCKEHFAENEKFTTIEKSTFRLTPTATQLLRTHINLFRKLSGILPVTDVAVELNKFAFMQLDNPEMKKREIDFCHGPLCGTGGLEAAVKEQQDGKCLLCGKESIGHYHHIVPRSRRGSNTIANIAGLCPKCHELVHKDADTAESLTEMKTGLMKKYGGTSVLNQIIPKLVETLADLFPGHFHVTNGWNTKEFREKHHLEKDHDVDAYCIACSHLKPEETLVETEPFEILQFRKHNRAIIHHQTERTYKLDGVTVAKNRKKRMEQKTDSLEDWYVDMAKEHGKTQADAMRSRLTVIKSTRYYNTPGRMMPGTVFLYEGKRYVMTGQITNGKYYRAYGQEKRNFPAVKVRILTKNTGLVFVAGSKRPAATKKAGQAKKKKGSYPYDVPDYAYPYDVPDYAYPYDVPDYA*"
     misc_feature    1911..1937
                     /label="SV40"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1944..2402
                     /label="OrufIscB"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1944..3734
                     /label="Translation 1944-3734"
                     /translation="RYVYVLDVDGKPLMPTCRFGKVRRMLKSGQAKAVDTLPFTIQLTYKPRTRILQPVTLGQDPGRTNIGMAAVRFDGKELGRFHCITRNKEIPKLMADRMAARKASRRGERLARKRLARKLHTTAKHLNGRILPGCSEPIAVKDIINTESRFNNRILTKCKVCGKNTPLRRNVRELLLENIVRFLPLESELKETLKRTILEGQQGNINKLFRKLKFNQKDWPGKNLTDIAKNKLPGRLPFCKEHFAENEKFTTIEKSTFRLTPTATQLLRTHINLFRKLSGILPVTDVAVELNKFAFMQLDNPEMKKREIDFCHGPLCGTGGLEAAVKEQQDGKCLLCGKESIGHYHHIVPRSRRGSNTIANIAGLCPKCHELVHKDADTAESLTEMKTGLMKKYGGTSVLNQIIPKLVETLADLFPGHFHVTNGWNTKEFREKHHLEKDHDVDAYCIACSHLKPEETLVETEPFEILQFRKHNRAIIHHQTERTYKLDGVTVAKNRKKRMEQKTDSLEDWYVDMAKEHGKTQADAMRSRLTVIKSTRYYNTPGRMMPGTVFLYEGKRYVMTGQITNGKYYRAYGQEKRNFPAVKVRILTKNTGLVFVA"
     misc_feature    2403..2717
                     /label="NbaCas9-1 REC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2643..2717
                     /label="REC 2L"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2718..3734
                     /label="OrufIscB"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3741..3788
                     /label="nucleoplasmin NLS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3795..3875
                     /label="3xHA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_site      3929..4153
                     /label="BGH pA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      4199..4627
                     /label="f1 origin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        4632..5001
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             5037..5831
                     /label="Neo(R)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    6005..6135
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      complement(6518..7188)
                     /label="pUC origin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(7333..849)
                     /label="Amp(R)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
       61 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      301 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      361 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      421 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      481 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      541 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      601 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      661 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      721 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      781 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      841 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      961 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     1021 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     1081 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     1141 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     1201 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     1261 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1321 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1381 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1441 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     1501 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     1561 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1621 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1681 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1741 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     1801 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     1861 tatagggaga cccaagctgg ctagcgttta aacttaagct tgccaccATG ggatcTCCAA
     1921 AAAAGAAACG GAAGGTGGGA TCCAGATACG TGTACGTGCT GGATGTGGAC GGCAAGCCTC
     1981 TGATGCCTAC ATGCAGGTTC GGCAAGGTGC GGAGAATGCT GAAATCTGGC CAAGCTAAGG
     2041 CCGTGGACAC CCTGCCTTTC ACCATCCAGC TGACCTACAA GCCCAGAACC AGAATCCTGC
     2101 AACCTGTGAC CCTGGGCCAG GACCCCGGCA GAACCAATAT CGGAATGGCC GCCGTGCGGT
     2161 TCGACGGCAA GGAACTGGGA AGATTCCACT GCATCACAAG AAACAAGGAA ATCCCTAAGC
     2221 TGATGGCCGA CAGAATGGCA GCCAGAAAGG CCTCCCGGAG AGGCGAGAGA CTGGCCAGAA
     2281 AGCGCCTGGC CCGGAAACTG CACACCACCG CCAAGCACCT GAATGGCAGA ATCCTGCCAG
     2341 GATGTAGCGA GCCCATCGCC GTGAAGGACA TCATCAACAC AGAGAGCAGA TTCAACAACA
     2401 GAATCCTGAC CAAATGCAAA GTGTGCGGCA AGAACACCCC TCTGAGAAGA AACGTGCGGG
     2461 AACTCCTGCT GGAAAACATC GTGCGGTTTC TGCCTCTGGA AAGCGAGCTG AAGGAAACAC
     2521 TCAAGAGAAC CATCCTGGAA GGCCAGCAGG GCAATATCAA CAAGCTGTTC AGGAAACTTA
     2581 AATTCAATCA AAAGGATTGG CCTGGCAAAA ACCTGACCGA TATCGCTAAG AATAAGCTGC
     2641 CAGGCCGGCT GCCCTTCTGC AAGGAGCACT TCGCCGAGAA CGAGAAGTTC ACCACCATCG
     2701 AGAAGAGCAC CTTCCGGCTG ACCCCTACCG CCACCCAGCT GCTGCGGACC CACATCAACC
     2761 TGTTTAGAAA GCTGAGCGGC ATCCTGCCCG TGACCGACGT GGCCGTCGAG CTGAACAAGT
     2821 TCGCCTTTAT GCAGCTGGAC AACCCTGAGA TGAAGAAGCG GGAAATCGAC TTCTGCCACG
     2881 GCCCACTGTG CGGCACCGGC GGCCTGGAAG CCGCTGTTAA GGAGCAGCAG GATGGAAAAT
     2941 GCCTGCTGTG TGGAAAAGAA TCTATCGGCC ACTACCACCA CATCGTGCCT AGGTCCCGTA
     3001 GAGGCAGCAA CACCATCGCT AATATCGCCG GCCTTTGTCC TAAGTGCCAC GAGCTCGTGC
     3061 ACAAGGACGC CGATACAGCC GAGTCTCTGA CAGAGATGAA AACCGGCCTG ATGAAGAAGT
     3121 ATGGAGGCAC CAGCGTGCTG AACCAGATCA TCCCCAAGCT GGTGGAAACC CTGGCCGACC
     3181 TGTTTCCTGG CCACTTCCAC GTGACAAACG GCTGGAACAC CAAGGAGTTC AGAGAGAAAC
     3241 ATCATCTGGA AAAGGATCAC GACGTGGATG CTTACTGCAT CGCCTGCAGC CACCTGAAGC
     3301 CTGAGGAAAC CCTGGTGGAA ACTGAGCCTT TCGAGATCCT GCAGTTCAGA AAGCACAACA
     3361 GAGCCATCAT TCACCACCAG ACCGAGCGGA CGTACAAGCT GGACGGCGTG ACAGTGGCTA
     3421 AAAATAGAAA GAAACGGATG GAACAGAAAA CCGACAGCCT GGAAGATTGG TACGTGGACA
     3481 TGGCCAAGGA ACACGGCAAA ACACAGGCCG ATGCCATGCG GAGCAGACTG ACCGTGATCA
     3541 AGAGCACACG GTATTACAAC ACCCCTGGCA GAATGATGCC AGGCACAGTT TTCCTGTACG
     3601 AGGGCAAGAG ATACGTGATG ACCGGCCAGA TTACAAACGG CAAGTACTAC AGAGCCTACG
     3661 GCCAGGAGAA GCGGAACTTC CCCGCTGTCA AGGTGAGGAT TCTGACAAAG AACACCGGAC
     3721 TAGTCTTTGT GGCCGGATCC aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa
     3781 agaaaaaggg atcTtaccca tacgatgttc cagattacgc ttacccatac gatgttccag
     3841 attacgctta cccatacgat gttccagatt acgctTGAgc ggccgctcga gtctagaggg
     3901 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3961 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4021 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4081 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4141 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4561 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4621 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4921 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4981 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     5041 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5101 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5161 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5221 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5281 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5341 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5401 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5461 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5521 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5581 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5641 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5701 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5761 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5821 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5881 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5941 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6001 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6061 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6121 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6181 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6241 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6301 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6361 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6421 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6481 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6541 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7081 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
     7141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7321 ttggtctgac agttaccaat gctt
//