LOCUS       pHS2002                 3072 bp ds-DNA     circular     09-MAY-2025
DEFINITION  .
COMMENT     ^^
FEATURES             Location/Qualifiers
     misc_feature    751..755
                     /label="RBS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    762..764
                     /label="Start codon"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        780..1028
                     /label="Human U6 Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1029..1051
                     /label="Golden gate site for spacer cloning"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          1031..1036
                     /label="pUC19"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1044..1049
                     /label="BbsI"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1052..1197
                     /label="NovaIscB omegaRNA (-17)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1053..1076
                     /label="guide adaptor hairpin"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1063..1066
                     /label="A12-A57 --> GAAA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1155..1197
                     /label="terminal hairpin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     source          complement(1617..1616)
                     /label="pUC19"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     -35_signal      1747..1752
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     -10_signal      1768..1773
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     sig_peptide     1817..1885
                     /label="bla"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1817..2677
                     /label="bla"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      2848..364
                     /label="pMB1 origin of replication"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     -35_signal      2848..2853
                     /label="RNAII promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     -10_signal      2869..2874
                     /label="RNAII promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_RNA        2884..364
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_RNA        complement(2887..2994)
                     /label="RNAI transcript"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     -10_signal      complement(3003..3008)
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     -35_signal      complement(3025..3030)
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
       61 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      121 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      181 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      241 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      301 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      361 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      421 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      481 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      541 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      601 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      661 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      721 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccag
      781 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      841 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      901 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      961 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1021 gaaacaccAT GTCTTCCTGG GACGAAGACA AGTCAATAAC CCGAAAGGGT TACTGAGCAG
     1081 AGCAGTGACA TGTCATTCGC CGCGGGGTGA TTCCAAGCTC CGCGCTCCGG CTAGACATGC
     1141 CCATGCTATG GAAACTTTAA CGGTATGTGC GGTTTTCCGC TCATACCGGC TTACAACTTT
     1201 TTTggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     1261 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     1321 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     1381 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     1441 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     1501 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1561 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     1621 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1681 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1741 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1801 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1861 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1921 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1981 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2041 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2101 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2161 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2221 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2281 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2341 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2401 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2461 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2521 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2581 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2641 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2701 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2761 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2821 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2881 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2941 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3001 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3061 tctgctaatc ct
//