LOCUS       pHS1623                 7578 bp ds-DNA     circular     09-MAY-2025
DEFINITION  .
KEYWORDS    "marker:AmpR, AmpR"
FEATURES             Location/Qualifiers
     primer_bind     310..329
                     /label="T7"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_binding    329..351
                     /label="LacO"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     RBS             368..390
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             397..900
                     /label="Translation 397-900"
                     /translation="MGSSHHHHSGHHHTGHHHHSGSHHHSSSGLVPRGSHMASWSHPQFEKGGGSGGGSGGSAWSHPQFEKGSAAGGEEDKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMTAIAFLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGGSSGS"
     CDS             409..474
                     /label="14xHIS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             484..501
                     /label="thrombin site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             514..537
                     /label="Twin-Strep-tag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             538..573
                     /label="Twin-Strep-tag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             574..597
                     /label="Twin-Strep-tag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             598..888
                     /label="bdSUMO"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             889..900
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    901..2394
                     /label="OrufIscB"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             901..2394
                     /label="Translation 901-2394"
                     /translation="RYVYVLDVDGKPLMPTCRFGKVRRMLKSGQAKAVDTLPFTIQLTYKPRTRILQPVTLGQDPGRTNIGMAAVRFDGKELGRFHCITRNKEIPKLMADRMAARKASRRGERLARKRLARKLHTTAKHLNGRILPGCSEPIAVKDIINTESRFNNRLRPEGWLTPTATQLLRTHINLFRKLSGILPVTDVAVELNKFAFMQLDNPEMKKREIDFCHGPLCGTGGLEAAVKEQQDGKCLLCGKESIGHYHHIVPRSRRGSNTIANIAGLCPKCHELVHKDADTAESLTEMKTGLMKKYGGTSVLNQIIPKLVETLADLFPGHFHVTNGWNTKEFREKHHLEKDHDVDAYCIACSHLKPEETLVETEPFEILQFRKHNRAIIHHQTERTYKLDGVTVAKNRKKRMEQKTDSLEDWYVDMAKEHGKTQADAMRSRLTVIKSTRYYNTPGRMMPGTVFLYEGKRYVMTGQITNGKYYRAYGQEKRNFPAVKVRILTKNTGLVFVA"
     CDS             901..2397
                     /label="Translation 901-2397"
                     /translation="RYVYVLDVDGKPLMPTCRFGKVRRMLKSGQAKAVDTLPFTIQLTYKPRTRILQPVTLGQDPGRTNIGMAAVRFDGKELGRFHCITRNKEIPKLMADRMAARKASRRGERLARKRLARKLHTTAKHLNGRILPGCSEPIAVKDIINTESRFNNRLRPEGWLTPTATQLLRTHINLFRKLSGILPVTDVAVELNKFAFMQLDNPEMKKREIDFCHGPLCGTGGLEAAVKEQQDGKCLLCGKESIGHYHHIVPRSRRGSNTIANIAGLCPKCHELVHKDADTAESLTEMKTGLMKKYGGTSVLNQIIPKLVETLADLFPGHFHVTNGWNTKEFREKHHLEKDHDVDAYCIACSHLKPEETLVETEPFEILQFRKHNRAIIHHQTERTYKLDGVTVAKNRKKRMEQKTDSLEDWYVDMAKEHGKTQADAMRSRLTVIKSTRYYNTPGRMMPGTVFLYEGKRYVMTGQITNGKYYRAYGQEKRNFPAVKVRILTKNTGLVFVA*"
     misc_feature    1078..1080
                     /label="RuvC-I"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1468..1470
                     /label="RuvC-II"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1639..1641
                     /label="HNH"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1927..1929
                     /label="RuvC-III'"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2398..900
                     /label="BB"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     terminator      2398..2445
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(2620..2625)
                     /label="tet promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(2626..2642)
                     /label="tet promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(2643..2648)
                     /label="tet promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        2762..2866
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             2867..2935
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2936..3727
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             3065..3724
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      3822..4504
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      3898..4486
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(6419..7501)
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     promoter        complement(7502..1)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
       61 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
      121 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
      181 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
      241 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
      301 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
      361 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcCA TCACCATCAT
      421 TCAGGCCATC ACCATACCGG ACACCACCAT CATTCAGGCA GTCATCACCA TTCCagcagc
      481 ggcctggtgc cgcgcggcag ccatatggct agctggagcc atccgcagtt tgaaaaaggt
      541 ggtggtagcg gtggtggttc aggtggtagt gcatggtcac accctcagtt tgagaaaGGA
      601 TCTGCTGCGG GTGGCGAAGA AGATAAGAAA CCGGCAGGTG GCGAAGGTGG CGGTGCCCAT
      661 ATCAACCTGA AAGTGAAAGG TCAAGACGGC AACGAAGTCT TTTTCCGCAT CAAACGTTCT
      721 ACCCAGCTGA AAAAGCTGAT GAACGCATAC TGTGACCGTC AGTCTGTAGA CATGACCGCA
      781 ATTGCTTTCC TGTTTGATGG TCGTCGCCTG CGTGCGGAAC AGACCCCGGA TGAACTGGAG
      841 ATGGAAGATG GCGACGAAAT CGACGCAATG CTGCACCAGA CTGGTGGCTC AAGTGGATCC
      901 AGATACGTGT ACGTGCTGGA TGTGGACGGC AAGCCTCTGA TGCCTACATG CAGGTTCGGC
      961 AAGGTGCGGA GAATGCTGAA ATCTGGCCAA GCTAAGGCCG TGGACACCCT GCCTTTCACC
     1021 ATCCAGCTGA CCTACAAGCC CAGAACCAGA ATCCTGCAAC CTGTGACCCT GGGCCAGGAC
     1081 CCCGGCAGAA CCAATATCGG AATGGCCGCC GTGCGGTTCG ACGGCAAGGA ACTGGGAAGA
     1141 TTCCACTGCA TCACAAGAAA CAAGGAAATC CCTAAGCTGA TGGCCGACAG AATGGCAGCC
     1201 AGAAAGGCCT CCCGGAGAGG CGAGAGACTG GCCAGAAAGC GCCTGGCCCG GAAACTGCAC
     1261 ACCACCGCCA AGCACCTGAA TGGCAGAATC CTGCCAGGAT GTAGCGAGCC CATCGCCGTG
     1321 AAGGACATCA TCAACACAGA GAGCAGATTC AACAACAGAC TGCGGCCTGA GGGATGGCTG
     1381 ACCCCTACCG CCACCCAGCT GCTGCGGACC CACATCAACC TGTTTAGAAA GCTGAGCGGC
     1441 ATCCTGCCCG TGACCGACGT GGCCGTCGAG CTGAACAAGT TCGCCTTTAT GCAGCTGGAC
     1501 AACCCTGAGA TGAAGAAGCG GGAAATCGAC TTCTGCCACG GCCCACTGTG CGGCACCGGC
     1561 GGCCTGGAAG CCGCTGTTAA GGAGCAGCAG GATGGAAAAT GCCTGCTGTG TGGAAAAGAA
     1621 TCTATCGGCC ACTACCACCA CATCGTGCCT AGGTCCCGTA GAGGCAGCAA CACCATCGCT
     1681 AATATCGCCG GCCTTTGTCC TAAGTGCCAC GAGCTCGTGC ACAAGGACGC CGATACAGCC
     1741 GAGTCTCTGA CAGAGATGAA AACCGGCCTG ATGAAGAAGT ATGGAGGCAC CAGCGTGCTG
     1801 AACCAGATCA TCCCCAAGCT GGTGGAAACC CTGGCCGACC TGTTTCCTGG CCACTTCCAC
     1861 GTGACAAACG GCTGGAACAC CAAGGAGTTC AGAGAGAAAC ATCATCTGGA AAAGGATCAC
     1921 GACGTGGATG CTTACTGCAT CGCCTGCAGC CACCTGAAGC CTGAGGAAAC CCTGGTGGAA
     1981 ACTGAGCCTT TCGAGATCCT GCAGTTCAGA AAGCACAACA GAGCCATCAT TCACCACCAG
     2041 ACCGAGCGGA CGTACAAGCT GGACGGCGTG ACAGTGGCTA AAAATAGAAA GAAACGGATG
     2101 GAACAGAAAA CCGACAGCCT GGAAGATTGG TACGTGGACA TGGCCAAGGA ACACGGCAAA
     2161 ACACAGGCCG ATGCCATGCG GAGCAGACTG ACCGTGATCA AGAGCACACG GTATTACAAC
     2221 ACCCCTGGCA GAATGATGCC AGGCACAGTT TTCCTGTACG AGGGCAAGAG ATACGTGATG
     2281 ACCGGCCAGA TTACAAACGG CAAGTACTAC AGAGCCTACG GCCAGGAGAA GCGGAACTTC
     2341 CCCGCTGTCA AGGTGAGGAT TCTGACAAAG AACACCGGAC TAGTCTTTGT GGCCtagcta
     2401 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     2461 atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
     2521 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
     2581 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa
     2641 gctgtcaaac atgagagatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     2701 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     2761 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2821 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     3121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     3421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3541 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3781 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3841 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3901 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3961 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4021 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4441 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4501 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4561 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4621 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     4681 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt
     4741 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     4801 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4861 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4921 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     4981 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     5041 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     5101 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     5161 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     5221 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     5281 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     5341 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     5401 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     5461 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     5521 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     5581 acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     5641 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     5701 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     5761 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     5821 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     5881 cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     5941 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     6001 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     6061 gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     6121 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     6181 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     6241 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     6301 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     6361 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     6421 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6481 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     6541 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     6601 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     6661 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     6721 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     6781 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     6841 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     6901 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     6961 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     7021 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     7081 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     7141 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     7201 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     7261 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     7321 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     7381 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     7441 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     7501 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     7561 tgcgccattc gatggtgt
//