LOCUS       pHS2096                 7923 bp ds-DNA     circular     09-MAY-2025
DEFINITION  .
KEYWORDS    "marker:AmpR, AmpR, AmpR"
FEATURES             Location/Qualifiers
     primer_bind     310..329
                     /label="T7"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="label: T7"
     misc_binding    329..351
                     /label="LacO"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: LacO"
     RBS             368..390
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: RBS"
     CDS             397..2742
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGSSHHHHSGHHHTGHHHHSGSHHHSSSGLVPRGSHMASWSHPQFEKGGGSGGGSGGSAWSHPQFEKGSAAGGEEDKKPAGGEGGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMTAIAFLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGGSSGSRYVYVLDVDGKPLMPTCRFGKVRRMLKSGQAKAVDTLPFTIQLTYKPRTRILQPVTLGQDPGRTNIGMAAVRFDGKELGRFHCITRNKEIPKLMADRMAARKASRRGERLARKRLARKLHTTAKHLNGRILPGCSEPIAVKDIINTESRFNNRILTKCKVCGKNTPLRRNVRELLLENIVKNIRLHGPGGEIVGLHPDLKETLKRTILEGQQGNINKLFRKLRKVYKITLNQKDWPGKNLTDIAKNKLPGRLPFCKEHFAENEKFTTIEKSTFRLTPTATQLLRTHINLFRKLSGILPVTDVAVELNKFAFMQLDNPEMKKREIDFCHGPLCGTGGLEAAVKEQQDGKCLLCGKESIGHYHHIVPRSRRGSNTIANIAGLCPKCHELVHKDADTAESLTEMKTGLMKKYGGTSVLNQIIPKLVETLADLFPGHFHVTNGWNTKEFREKHHLEKDHDVDAYCIACSHLKPEETLVETEPFEILQFRKHNRAIIHHQTERTYKLDGVTVAKNRKKRMEQKTDSLEDWYVDMAKEHGKTQADAMRSRLTVIKSTRYYNTPGRMMPGTVFLYEGKRYVMTGQITNGKYYRAYGQEKRNFPAVKVRILTKNTGLVFVA*"
     CDS             409..474
                     /label="14xHIS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: 14xHIS"
     CDS             484..501
                     /label="thrombin site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: thrombin site"
     CDS             514..537
                     /label="Twin-Strep-tag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: Twin-Strep-tag"
     CDS             538..573
                     /label="Twin-Strep-tag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: Twin-Strep-tag"
     CDS             574..597
                     /label="Twin-Strep-tag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: Twin-Strep-tag"
     CDS             598..888
                     /label="bdSUMO"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: bdSUMO"
     CDS             889..900
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: Linker"
     misc_feature    901..1359
                     /label="OrufIscB"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="label: Rd8_!17"
     misc_feature    1360..1722
                     /label="NbaCas9-1 REC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1441..1494
                     /label="swap 11"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1567..1608
                     /label="swap 49"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1723..2739
                     /label="OrufIscB"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: Rd8_!17"
     misc_feature    2743..900
                     /label="BB"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="label: BB"
     terminator      2743..2790
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: T7 terminator"
     promoter        complement(2965..2970)
                     /label="tet promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: tet promoter"
     promoter        complement(2971..2987)
                     /label="tet promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: tet promoter"
     promoter        complement(2988..2993)
                     /label="tet promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: tet promoter"
     promoter        3107..3211
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: AmpR promoter"
     CDS             3212..3280
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: AmpR"
     CDS             3281..4072
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: AmpR"
     CDS             3410..4069
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: AmpR"
     rep_origin      4167..4849
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="label: ColE1 origin"
     rep_origin      4243..4831
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: ori"
     CDS             complement(6764..7846)
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="label: lacI"
     promoter        complement(7847..1)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: lacI promoter"
ORIGIN
        1 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
       61 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
      121 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
      181 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
      241 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
      301 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
      361 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcCA TCACCATCAT
      421 TCAGGCCATC ACCATACCGG ACACCACCAT CATTCAGGCA GTCATCACCA TTCCagcagc
      481 ggcctggtgc cgcgcggcag ccatatggct agctggagcc atccgcagtt tgaaaaaggt
      541 ggtggtagcg gtggtggttc aggtggtagt gcatggtcac accctcagtt tgagaaaGGA
      601 TCTGCTGCGG GTGGCGAAGA AGATAAGAAA CCGGCAGGTG GCGAAGGTGG CGGTGCCCAT
      661 ATCAACCTGA AAGTGAAAGG TCAAGACGGC AACGAAGTCT TTTTCCGCAT CAAACGTTCT
      721 ACCCAGCTGA AAAAGCTGAT GAACGCATAC TGTGACCGTC AGTCTGTAGA CATGACCGCA
      781 ATTGCTTTCC TGTTTGATGG TCGTCGCCTG CGTGCGGAAC AGACCCCGGA TGAACTGGAG
      841 ATGGAAGATG GCGACGAAAT CGACGCAATG CTGCACCAGA CTGGTGGCTC AAGTGGATCC
      901 AGATACGTGT ACGTGCTGGA TGTGGACGGC AAGCCTCTGA TGCCTACATG CAGGTTCGGC
      961 AAGGTGCGGA GAATGCTGAA ATCTGGCCAA GCTAAGGCCG TGGACACCCT GCCTTTCACC
     1021 ATCCAGCTGA CCTACAAGCC CAGAACCAGA ATCCTGCAAC CTGTGACCCT GGGCCAGGAC
     1081 CCCGGCAGAA CCAATATCGG AATGGCCGCC GTGCGGTTCG ACGGCAAGGA ACTGGGAAGA
     1141 TTCCACTGCA TCACAAGAAA CAAGGAAATC CCTAAGCTGA TGGCCGACAG AATGGCAGCC
     1201 AGAAAGGCCT CCCGGAGAGG CGAGAGACTG GCCAGAAAGC GCCTGGCCCG GAAACTGCAC
     1261 ACCACCGCCA AGCACCTGAA TGGCAGAATC CTGCCAGGAT GTAGCGAGCC CATCGCCGTG
     1321 AAGGACATCA TCAACACAGA GAGCAGATTC AACAACAGAA TCCTGACCAA ATGCAAAGTG
     1381 TGCGGCAAGA ACACCCCTCT GAGAAGAAAC GTGCGGGAAC TCCTGCTGGA AAACATCGTG
     1441 AAGAACATCA GACTGCACGG CCCTGGCGGA GAGATCGTGG GCCTGCACCC CGACCTGAAG
     1501 GAAACACTCA AGAGAACCAT CCTGGAAGGC CAGCAGGGCA ATATCAACAA GCTGTTCAGG
     1561 AAACTTAGAA AGGTGTACAA GATCACCCTG AACCAGAAAG ACTGGCCTGG CAAAAACCTG
     1621 ACCGATATCG CTAAGAATAA GCTGCCAGGC CGGCTGCCCT TCTGCAAGGA GCACTTCGCC
     1681 GAGAACGAGA AGTTCACCAC CATCGAGAAG AGCACCTTCC GGCTGACCCC TACCGCCACC
     1741 CAGCTGCTGC GGACCCACAT CAACCTGTTT AGAAAGCTGA GCGGCATCCT GCCCGTGACC
     1801 GACGTGGCCG TCGAGCTGAA CAAGTTCGCC TTTATGCAGC TGGACAACCC TGAGATGAAG
     1861 AAGCGGGAAA TCGACTTCTG CCACGGCCCA CTGTGCGGCA CCGGCGGCCT GGAAGCCGCT
     1921 GTTAAGGAGC AGCAGGATGG AAAATGCCTG CTGTGTGGAA AAGAATCTAT CGGCCACTAC
     1981 CACCACATCG TGCCTAGGTC CCGTAGAGGC AGCAACACCA TCGCTAATAT CGCCGGCCTT
     2041 TGTCCTAAGT GCCACGAGCT CGTGCACAAG GACGCCGATA CAGCCGAGTC TCTGACAGAG
     2101 ATGAAAACCG GCCTGATGAA GAAGTATGGA GGCACCAGCG TGCTGAACCA GATCATCCCC
     2161 AAGCTGGTGG AAACCCTGGC CGACCTGTTT CCTGGCCACT TCCACGTGAC AAACGGCTGG
     2221 AACACCAAGG AGTTCAGAGA GAAACATCAT CTGGAAAAGG ATCACGACGT GGATGCTTAC
     2281 TGCATCGCCT GCAGCCACCT GAAGCCTGAG GAAACCCTGG TGGAAACTGA GCCTTTCGAG
     2341 ATCCTGCAGT TCAGAAAGCA CAACAGAGCC ATCATTCACC ACCAGACCGA GCGGACGTAC
     2401 AAGCTGGACG GCGTGACAGT GGCTAAAAAT AGAAAGAAAC GGATGGAACA GAAAACCGAC
     2461 AGCCTGGAAG ATTGGTACGT GGACATGGCC AAGGAACACG GCAAAACACA GGCCGATGCC
     2521 ATGCGGAGCA GACTGACCGT GATCAAGAGC ACACGGTATT ACAACACCCC TGGCAGAATG
     2581 ATGCCAGGCA CAGTTTTCCT GTACGAGGGC AAGAGATACG TGATGACCGG CCAGATTACA
     2641 AACGGCAAGT ACTACAGAGC CTACGGCCAG GAGAAGCGGA ACTTCCCCGC TGTCAAGGTG
     2701 AGGATTCTGA CAAAGAACAC CGGACTAGTC TTTGTGGCCt agctagcata accccttggg
     2761 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatccc
     2821 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     2881 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     2941 aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag
     3001 agattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3061 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3421 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     3481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3721 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     3781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4081 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4141 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4201 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4261 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4321 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4381 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4441 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4501 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4561 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4621 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4681 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4741 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4801 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4861 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4921 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4981 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     5041 gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat
     5101 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     5161 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5221 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5281 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     5341 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     5401 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     5461 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     5521 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     5581 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     5641 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     5701 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     5761 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     5821 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     5881 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg
     5941 agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
     6001 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
     6061 atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc
     6121 gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc
     6181 gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga
     6241 agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac
     6301 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
     6361 cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc
     6421 gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
     6481 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
     6541 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
     6601 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     6661 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
     6721 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
     6781 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6841 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     6901 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     6961 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
     7021 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
     7081 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     7141 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     7201 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     7261 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     7321 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     7381 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     7441 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     7501 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     7561 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     7621 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     7681 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     7741 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     7801 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     7861 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     7921 tgt
//