LOCUS       pET-29(+)_C3-TJ04-FKBP  6237 bp ds-DNA     circular     19-MAY-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        145..222
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: lacI note: "
     CDS             223..1305
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). product: lac repressor transl_table: 1 translation: VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             2114..2305
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: rop product: Rop protein transl_table: 1 translation: VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL*"
     rep_origin      complement(2735..3323)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             3445..4260
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-Ia note: confers resistance to kanamycin product: aminoglycoside phosphotransferase transl_table: 1 translation: MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     rep_origin      complement(4353..4808)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     terminator      4845..4892
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: transcription terminator for bacteriophage T7 RNA polymerase"
     CDS             complement(4959..4976)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     misc_feature    4977..4977
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: multiple cloning site"
     CDS             complement(4983..5981)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MRGHHHHHHGSSGDECEEKARRVAEKVERLKRSGTSEDEIAEEVAREISEVIRTLKESGSEYKVICRCVARIVAEIVEALKRSGTSEDEIAEIVARVISEVIRTLKESGSDYLIICVCVAIIVAEIVEALKRSGTSEDEIAEIVARVISEVIRTLKESGSSYEVIKECVQIIVLAIILALMKSGTEVEEILLILLRVKTEVRRTLKESGSGGSGGSGGSGGSGGSGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE*"
     CDS             complement(5981..5981)
                     /label="ATG"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: start codon transl_table: 1 translation: M"
     RBS             5990..5995
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: ribosome binding site"
     protein_bind    6026..6050
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6051..6069)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA CCATTCCTTG
       61 CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC
      121 ATAAGGGAGA GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG
      181 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA
      241 TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG
      301 GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT
      361 TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT
      421 GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC
      481 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC
      541 TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT
      601 CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA
      661 TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT
      721 ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG
      781 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT
      841 CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT
      901 CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC
      961 GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG
     1021 GACATCTCGG TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA
     1081 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA
     1141 CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT GGTGAAAAGA
     1201 AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA
     1261 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA
     1321 TGTAAGTTAG CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA
     1381 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT
     1441 CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTGGGTCA TTTTCGGCGA
     1501 GGACCGCTTT CGCTGGAGCG CGACGATGAT CGGCCTGTCG CTTGCGGTAT TCGGAATCTT
     1561 GCACGCCCTC GCTCAAGCCT TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA
     1621 GGCCATTATC GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG
     1681 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC CGATACGCGA
     1741 GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC TGAGCAACAA CATGAATGGT
     1801 CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA AACGCGGAAG TCAGCGCCCT GCACCATTAT
     1861 GTTCCGGATC TGCATCGCAG GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT
     1921 AACGAAGCGC TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC
     1981 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG TAACCCGTAT
     2041 CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC ATGAACAGAA ATCCCCCTTA
     2101 CACGGAGGCA TCAGTGACCA AACAGGAAAA AACCGCCCTT AACATGGCCC GCTTTATCAG
     2161 AAGCCAGACA TTAACGCTTC TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA
     2221 CATCTGTGAA TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT
     2281 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT
     2341 GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG
     2401 TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACTG GCTTAACTAT
     2461 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA
     2521 GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
     2581 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
     2641 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
     2701 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
     2761 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
     2821 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
     2881 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT
     2941 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
     3001 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
     3061 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
     3121 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG
     3181 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
     3241 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
     3301 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
     3361 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC
     3421 ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG
     3481 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT
     3541 GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG
     3601 TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA
     3661 AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT
     3721 GATGCATGGT TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA
     3781 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT
     3841 TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG
     3901 CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC
     3961 TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA
     4021 GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA
     4081 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA
     4141 TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT
     4201 ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA
     4261 GAATTAATTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     4321 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA
     4381 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA
     4441 AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA
     4501 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA
     4561 GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG
     4621 TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC
     4681 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC
     4741 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
     4801 GGGCGCGTCC CATTCGCCAA TCCGGATATA GTTCCTCCTT TCAGCAAAAA ACCCCTCAAG
     4861 ACCCGTTTAG AGGCCCCAAG GGGTTATGCT AGTTATTGCT CAGCGGTGGC AGCAGCCAAC
     4921 TCAGCTTCCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGCTCG
     4981 AGTTATTCCA GTTTCAGCAG TTCCACATCA AACACCAGAG TCGCATGTGG TGGAATGATG
     5041 CCCGGATGGC CGGTCGCGCC ATACGCATAA TCCGGGCTAA TGGTCAGTTT TGCACGTTGG
     5101 CCAACGCTCA TCTGGGCCAC GCCTTCTTCC CAGCCGCGAA TCACTTCCTG TTTGCCCAAC
     5161 ATAAATTTAA ACGGTTTGTT GCGATCGCGG CTGCTATCAA ACTTTTTACC ATCTTCCAGC
     5221 ATACCGGTGT AGTGCACAAC ACAGGTCTGG CCACGTTTCG GAAAGGTACG GCCATCACCC
     5281 GGGCTGATGG TTTCTACCTG CACTCCGCTT CCTCCGGATC CACCACTTCC ACCAGACCCA
     5341 CCGCTACCAC CACTACCCGA TTCTTTCAGT GTGCGGCGCA CTTCGGTTTT AACACGCAAC
     5401 AGAATCAGCA GAATTTCTTC AACCTCGGTG CCGCTTTTCA TCAGCGCCAG AATAATCGCC
     5461 AGCACAATAA TCTGCACGCA TTCTTTGATT ACTTCATAGC TTGAACCGCT TTCTTTTAAG
     5521 GTGCGAATGA CTTCGCTGAT CACACGCGCC ACAATTTCTG CGATTTCGTC TTCAGAGGTC
     5581 CCGCTCCGTT TCAACGCTTC GACAATTTCA GCGACAATGA TCGCAACGCA AACGCAAATA
     5641 ATCAGATAAT CGGAACCGGA TTCTTTAAGC GTGCGAATAA CTTCAGAAAT TACGCGCGCA
     5701 ACAATTTCCG CGATCTCATC TTCGCTCGTG CCAGAACGTT TCAGGGCTTC CACGATTTCG
     5761 GCCACAATAC GTGCCACGCA GCGGCAGATC ACTTTATATT CGCTGCCGCT CTCTTTCAGG
     5821 GTGCGGATCA CCTCGCTAAT TTCGCGCGCC ACTTCTTCGG CAATTTCATC CTCCGAGGTA
     5881 CCGCTGCGTT TCAGACGTTC CACTTTTTCC GCAACACGGC GCGCTTTTTC TTCGCATTCA
     5941 TCGCCTGAGC TGCCATGATG GTGGTGATGA TGACCGCGCA TATGTATATC TCCTTCTTAA
     6001 AGTTAAACAA AATTATTTCT AGAGGGGAAT TGTTATCCGC TCACAATTCC CCTATAGTGA
     6061 GTCGTATTAA TTTCGCGGGA TCGAGATCGA TCTCGATCCT CTACGCCGGA CGCATCGTGG
     6121 CCGGCATCAC CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG
     6181 GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGG
//