LOCUS       pET-29(+)_C6-d1-1-FKBP  5916 bp ds-DNA     circular     19-MAY-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      26..73
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: transcription terminator for bacteriophage T7 RNA polymerase"
     CDS             complement(140..157)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     misc_feature    158..158
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: multiple cloning site"
     CDS             complement(841..841)
                     /label="ATG"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: start codon transl_table: 1 translation: M"
     RBS             850..855
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: ribosome binding site"
     protein_bind    886..910
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(911..929)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     promoter        1242..1319
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: lacI note: "
     CDS             1320..2402
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). product: lac repressor transl_table: 1 translation: VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             3211..3402
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: rop product: Rop protein transl_table: 1 translation: VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL*"
     rep_origin      complement(3832..4420)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             4542..5357
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-Ia note: confers resistance to kanamycin product: aminoglycoside phosphotransferase transl_table: 1 translation: MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     rep_origin      complement(5450..5905)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTTATTCC AATTTCAGCA
      181 GTTCCACATC AAACACCAGG GTCGCATGTG GTGGAATGAT ACCCGGATGG CCGGTCGCGC
      241 CATACGCATA ATCCGGTGAG ATGGTCAGTT TTGCACGCTG ACCAACGCTC ATCTGCGCCA
      301 CACCTTCTTC CCAGCCACGA ATAACTTCCT GTTTGCCCAG CATGAATTTA AACGGTTTGT
      361 TGCGATCGCG GCTGCTATCA AACTTTTTAC CATCTTCCAG CATACCTGTA TAATGCACAA
      421 CGCAGGTTTG GCCGCGTTTC GGAAAGGTAC GACCATCGCC CGGGCTGATG GTTTCAACCT
      481 GCACACCCGA TCCACCACTT CCTCCGCTTC CCCCAGAACC ACCGCTACCA CCAGAAAAGC
      541 TAAGGCTTAA GCTGACGCCT TCAATATCCG CAATTTCCAG GCACACTTTA AACACTTCCA
      601 GTTTTTCGAC GCTAACAGAC AGGCTAATGC TCTGGCCTTT TTCCGCCAGC TTTTTAACCG
      661 CTTCTTTTAA AATTTCCGCG ACTTCCACTT TCTTCAGGCT GAAGCTCAAG CTCAGTTCGC
      721 TCGCATCCAC GCTAAGAATC GCTTCCATGG CTTCTTTCAT ATCTTCTGCT TTTTCAAAGC
      781 TGCCGCTCAC GCTCAGGCTT TTGCCGCTGC TGCCGTGATG GTGGTGATGA TGACCGCGCA
      841 TATGTATATC TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGGAAT TGTTATCCGC
      901 TCACAATTCC CCTATAGTGA GTCGTATTAA TTTCGCGGGA TCGAGATCGA TCTCGATCCT
      961 CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG CTGGCGCCTA
     1021 TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG
     1081 TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG CCATCTCCTT
     1141 GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC TGGGCTGCTT
     1201 CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAGATCCC GGACACCATC GAATGGCGCA
     1261 AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG
     1321 TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT
     1381 CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG
     1441 CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT
     1501 CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG
     1561 CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC
     1621 GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG
     1681 GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA
     1741 CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT
     1801 TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG GGTCACCAGC
     1861 AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT CTGGCTGGCT
     1921 GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA GGCGACTGGA
     1981 GTGCCATGTC CGGTTTTCAA CAAACCATGC AAATGCTGAA TGAGGGCATC GTTCCCACTG
     2041 CGATGCTGGT TGCCAACGAT CAGATGGCGC TGGGCGCAAT GCGCGCCATT ACCGAGTCCG
     2101 GGCTGCGCGT TGGTGCGGAC ATCTCGGTAG TGGGATACGA CGATACCGAA GACAGCTCAT
     2161 GTTATATCCC GCCGTTAACC ACCATCAAAC AGGATTTTCG CCTGCTGGGG CAAACCAGCG
     2221 TGGACCGCTT GCTGCAACTC TCTCAGGGCC AGGCGGTGAA GGGCAATCAG CTGTTGCCCG
     2281 TCTCACTGGT GAAAAGAAAA ACCACCCTGG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
     2341 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT
     2401 GAGCGCAACG CAATTAATGT AAGTTAGCTC ACTCATTAGG CACCGGGATC TCGACCGATG
     2461 CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC
     2521 GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC
     2581 TGGGTCATTT TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA CGATGATCGG CCTGTCGCTT
     2641 GCGGTATTCG GAATCTTGCA CGCCCTCGCT CAAGCCTTCG TCACTGGTCC CGCCACCAAA
     2701 CGTTTCGGCG AGAAGCAGGC CATTATCGCC GGCATGGCGG CCCCACGGGT GCGCATGATC
     2761 GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG TTAGCAGAAT
     2821 GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT GCAAAACGTC TGCGACCTGA
     2881 GCAACAACAT GAATGGTCTT CGGTTTCCGT GTTTCGTAAA GTCTGGAAAC GCGGAAGTCA
     2941 GCGCCCTGCA CCATTATGTT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA
     3001 ACACCTACAT CTGTATTAAC GAAGCGCTGG CATTGACCCT GAGTGATTTT TCTCTGGTCC
     3061 CGCCGCATCC ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG GGCATGTTCA
     3121 TCATCAGTAA CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT TACCCCCATG
     3181 AACAGAAATC CCCCTTACAC GGAGGCATCA GTGACCAAAC AGGAAAAAAC CGCCCTTAAC
     3241 ATGGCCCGCT TTATCAGAAG CCAGACATTA ACGCTTCTGG AGAAACTCAA CGAGCTGGAC
     3301 GCGGATGAAC AGGCAGACAT CTGTGAATCG CTTCACGACC ACGCTGATGA GCTTTACCGC
     3361 AGCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
     3421 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA
     3481 GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG
     3541 TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATATGCG
     3601 GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC
     3661 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
     3721 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
     3781 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
     3841 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     3901 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     3961 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     4021 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     4081 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     4141 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     4201 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     4261 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     4321 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     4381 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     4441 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAACAA
     4501 TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG
     4561 AAACGTCTTG CTCTAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA
     4621 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGATTG TATGGGAAGC
     4681 CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG
     4741 ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT
     4801 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGG AAAACAGCAT
     4861 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT
     4921 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT
     4981 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG
     5041 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC
     5101 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG
     5161 ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC
     5221 AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC
     5281 TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC
     5341 TCGATGAGTT TTTCTAAGAA TTAATTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     5401 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA AATTGTAAAC
     5461 GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA
     5521 TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT
     5581 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG
     5641 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT
     5701 TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA
     5761 GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG
     5821 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG
     5881 CTTAATGCGC CGCTACAGGG CGCGTCCCAT TCGCCA
//