LOCUS       pET-29(+)_T3-ank-FKBP   6342 bp ds-DNA     circular     19-MAY-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      26..73
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: transcription terminator for bacteriophage T7 RNA polymerase"
     CDS             complement(140..157)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     misc_feature    158..158
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: multiple cloning site"
     CDS             complement(1267..1267)
                     /label="ATG"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: start codon transl_table: 1 translation: M"
     RBS             1276..1281
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: ribosome binding site"
     protein_bind    1312..1336
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1337..1355)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     promoter        1668..1745
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: lacI note: "
     CDS             1746..2828
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). product: lac repressor transl_table: 1 translation: VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             3637..3828
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: rop product: Rop protein transl_table: 1 translation: VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL*"
     rep_origin      complement(4258..4846)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             4968..5783
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-Ia note: confers resistance to kanamycin product: aminoglycoside phosphotransferase transl_table: 1 translation: MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     rep_origin      complement(5876..6331)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTTATTCC AGTTTCAACA
      181 GTTCCACATC AAAAACCAGA GTCGCATGTG GCGGAATGAT ACCCGGATGG CCGGTCGCAC
      241 CATACGCATA ATCCGGGCTA ATGGTCAGTT TTGCGCGCTG ACCCACGCTC ATTTGCGCCA
      301 CGCCTTCTTC CCAGCCACGA ATCACTTCCT GTTTGCCCAG CATAAATTTA AACGGTTTGT
      361 TGCGATCGCG GCTGCTATCA AACTTTTTAC CGTCTTCCAA CATACCGGTA TAATGCACAA
      421 CGCAGGTCTG GCCGCGTTTC GGAAACGTAC GACCATCACC CGGGCTGATG GTTTCCACCT
      481 GCACACCGGA ACCACCACTA CCACCCGAAC CACCGCTACC ACCAGAACCG CCTTGTTTTT
      541 CCAGCGCTTT CACCACTTCT TCGTTGCCAT GTTCACGGGC CAGATCCAGC GGGGTTCTGC
      601 CATCGGAATC GCTGGTGTTT ACATCTGCGC CCGCCAGGAT CAGAACCAGT ACCACACGTT
      661 TATGGCCGTT TTCTGCGGCG TGGTGCAGAG GGGTGCGGCC ATCTGAATCT TTGGCGTTGA
      721 CATCCGCGCC TTTCAGCAGC AGAATCAGCA CTACTTCATC ATGTCCGTTT TCCGCGGCAT
      781 GGTGCAACGG GGTACGGCCA TCGCTGTCTT TTTCATTAAC ATCGGCGCCT TTTTCGATCA
      841 GCAGGGTCAC AACTTCTGCA TGACCATTTT CAGCCGCGTG ATGCAGCGGT GTGCGACCGT
      901 CGCTATCCTT TTCGTTCACA TCCGCACCCG CATCGATCGC CACTTTAACC ACAGCTGCAG
      961 CACCAGCTTC CGCCGCATGA TGCAAAGGAG TGCGCCCATC ACTATCTTTC GCGTTCGCAT
     1021 CTGCACCCTG GTTCATGGCG CTAATCGCCT GGTTGGCCAC GCTGGCTTCA ATCGCTTTGA
     1081 CAATAACTTT AAGCACTTTC ACAATGGTAT CTTTATCCGG GTTCTTTTCC AGGCGTTCCA
     1141 GCATTTCTTT CAGTTCCGCC AGAATGCGAA TCAGTTCAAT CAGGCTATCT TCCAGCTGTT
     1201 TCAGCAGTTC CTTTTTCTTT TCTTCATCGC CCGAGCTACC GTGATGGTGA TGATGATGAC
     1261 CGCGCATATG TATATCTCCT TCTTAAAGTT AAACAAAATT ATTTCTAGAG GGGAATTGTT
     1321 ATCCGCTCAC AATTCCCCTA TAGTGAGTCG TATTAATTTC GCGGGATCGA GATCGATCTC
     1381 GATCCTCTAC GCCGGACGCA TCGTGGCCGG CATCACCGGC GCCACAGGTG CGGTTGCTGG
     1441 CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT CGCCACTTCG GGCTCATGAG
     1501 CGCTTGTTTC GGCGTGGGTA TGGTGGCAGG CCCCGTGGCC GGGGGACTGT TGGGCGCCAT
     1561 CTCCTTGCAT GCACCATTCC TTGCGGCGGC GGTGCTCAAC GGCCTCAACC TACTACTGGG
     1621 CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA GATCCCGGAC ACCATCGAAT
     1681 GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG
     1741 TGAATGTGAA ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA
     1801 CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG
     1861 AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA
     1921 AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA
     1981 TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG
     2041 TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG
     2101 TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG
     2161 CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA
     2221 TTATTTTCTC CCATGAAGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC
     2281 ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG
     2341 CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC GATAGCGGAA CGGGAAGGCG
     2401 ACTGGAGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT GCTGAATGAG GGCATCGTTC
     2461 CCACTGCGAT GCTGGTTGCC AACGATCAGA TGGCGCTGGG CGCAATGCGC GCCATTACCG
     2521 AGTCCGGGCT GCGCGTTGGT GCGGACATCT CGGTAGTGGG ATACGACGAT ACCGAAGACA
     2581 GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA TTTTCGCCTG CTGGGGCAAA
     2641 CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC GGTGAAGGGC AATCAGCTGT
     2701 TGCCCGTCTC ACTGGTGAAA AGAAAAACCA CCCTGGCGCC CAATACGCAA ACCGCCTCTC
     2761 CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG
     2821 GGCAGTGAGC GCAACGCAAT TAATGTAAGT TAGCTCACTC ATTAGGCACC GGGATCTCGA
     2881 CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT
     2941 ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA
     3001 GCGCTCTGGG TCATTTTCGG CGAGGACCGC TTTCGCTGGA GCGCGACGAT GATCGGCCTG
     3061 TCGCTTGCGG TATTCGGAAT CTTGCACGCC CTCGCTCAAG CCTTCGTCAC TGGTCCCGCC
     3121 ACCAAACGTT TCGGCGAGAA GCAGGCCATT ATCGCCGGCA TGGCGGCCCC ACGGGTGCGC
     3181 ATGATCGTGC TCCTGTCGTT GAGGACCCGG CTAGGCTGGC GGGGTTGCCT TACTGGTTAG
     3241 CAGAATGAAT CACCGATACG CGAGCGAACG TGAAGCGACT GCTGCTGCAA AACGTCTGCG
     3301 ACCTGAGCAA CAACATGAAT GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT GGAAACGCGG
     3361 AAGTCAGCGC CCTGCACCAT TATGTTCCGG ATCTGCATCG CAGGATGCTG CTGGCTACCC
     3421 TGTGGAACAC CTACATCTGT ATTAACGAAG CGCTGGCATT GACCCTGAGT GATTTTTCTC
     3481 TGGTCCCGCC GCATCCATAC CGCCAGTTGT TTACCCTCAC AACGTTCCAG TAACCGGGCA
     3541 TGTTCATCAT CAGTAACCCG TATCGTGAGC ATCCTCTCTC GTTTCATCGG TATCATTACC
     3601 CCCATGAACA GAAATCCCCC TTACACGGAG GCATCAGTGA CCAAACAGGA AAAAACCGCC
     3661 CTTAACATGG CCCGCTTTAT CAGAAGCCAG ACATTAACGC TTCTGGAGAA ACTCAACGAG
     3721 CTGGACGCGG ATGAACAGGC AGACATCTGT GAATCGCTTC ACGACCACGC TGATGAGCTT
     3781 TACCGCAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
     3841 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
     3901 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC
     3961 GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA
     4021 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC
     4081 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
     4141 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
     4201 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
     4261 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     4321 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     4381 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     4441 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     4501 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     4561 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     4621 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     4681 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     4741 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     4801 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     4861 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     4921 GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC
     4981 AACGGGAAAC GTCTTGCTCT AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG
     5041 GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGATTGTATG
     5101 GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG
     5161 TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA
     5221 AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGGAAAA
     5281 CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG
     5341 CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC
     5401 GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG
     5461 ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC
     5521 TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA
     5581 TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC
     5641 GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA
     5701 AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT
     5761 TGATGCTCGA TGAGTTTTTC TAAGAATTAA TTCATGAGCG GATACATATT TGAATGTATT
     5821 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGAAATT
     5881 GTAAACGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT
     5941 AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG
     6001 TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC
     6061 AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA
     6121 AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA
     6181 TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA
     6241 GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC
     6301 GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CA
//