LOCUS       Exported                5807 bp ds-DNA     circular SYN 14-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   VB250211-1201dud
VERSION     .
KEYWORDS    VB250211-1201dud.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 14, 2025 from SnapGene 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="synthetic DNA construct"
     source          1376..2179
                     /label=source
     misc_feature    169..1044
                     /label=Full Enh98
     misc_feature    359..858
                     /label=Enh098 500bp Peak
     misc_feature    478..757
                     /label=Core
     promoter        1074..1122
                     /label=pB-Globin
     intron          1207..1339
                     /label=Intron
     misc_feature    1376..1381
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             1430..1450
                     /label=SV40 NLS
     CDS             1460..2179
                     /label=EGFP
     misc_feature    2216..2813
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packaging
                     cells, leading to higher titer of packaged virus; enhances 
                     higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional
                     regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional
                     regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     misc_feature    2844..3051
                     /label=BGH pA
                     /label=nonstandard type:  Regulatory_Elem
                     /note="/application_notes=Allows transcription termination 
                     and
                     polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
     repeat_region   3059..3199
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        4011..4115
                     /label=AmpR promoter
     CDS             4116..4976
                     /label=Ampicillin
                     /label=AmpiciIIin
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
     CDS             4116..4184
                     /label=AmpR
     rep_origin      5147..5735
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
     repeat_region   join(5797..5807,1..130)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgacg cgtttaatac cgtggcctta
      181 gtctgataaa ccaaaacctg ctccattatg aatcagtgct gtggggagtg ggtagagagt
      241 gtgaagttct ggggtggggg agtctggaga gagggtggga gcagccattc tgcagcagtg
      301 ccttcttggg gtcatgggtc tgtaggtgct gctgtggagg gagagatcag cctattctgg
      361 cttcatttct gagctgcaaa ctgcctgggt gtctggagaa gcaggttggc gtggtggtta
      421 gcagtgcgtg ggcggggttg cccgctcttg atttatgatt tctttgtctc tgtggaagca
      481 cttaagtgca ggctttagtt ccaatgacac tcaggagcct ctggattcca gcactgggga
      541 tgggggtggg gtagaacgtt ctcaggcctc accaacccct cccctgtgtg ctgcctttgg
      601 gagagtccca aggcttcagc attacttaat taattaggcc tctactgcta cataggctca
      661 gattcaaaag aacagagtgg cccacgtcag ccattcccgg aaaagtctga tggctggaag
      721 ccagaggact atgtgtctgc cttgctgccc ttggccagcc catcctgaat gcccagactc
      781 ggacaatgga gtaggtacag aagggtaaag acagtgtctt ctgtaccagt aagtgggccc
      841 tgatctgctc tctacagctt ccagagaaag ggcctggcca atgagcggcc ttttgagtag
      901 cagatacctc acatgcattc tgatagaaag cctggcccca gatcactgtg actttagccc
      961 tcaggtttct tttgcacttc aattcaatga cttcttgagg ttcatttccc tctccaagat
     1021 ttgccacaga ccagtggttc tcaagtcgac agatctaatt cctgcagccc gggctgggca
     1081 taaaagtcag ggcagagcca tctattgctt acatttgctt ctagcctgca ggtcgaggag
     1141 cgcagccttc cagaagcaga gcgcggcgcc ttaagctgca gaagttggtc gtgaggcact
     1201 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
     1261 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
     1321 tttgcctttc tctccacagg tgtccggtac ccaagtttgt acaaaaaagc aggctgccac
     1381 catgtctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     1441 gagaaaggta gaagacccca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1501 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1561 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1621 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1681 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1741 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1801 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1861 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1921 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1981 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2041 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     2101 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2161 ggacgagctg tacaagtaaa agcttaccca gctttcttgt acaaagtggg aattccgata
     2221 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2281 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2341 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2401 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2461 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2521 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2581 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     2641 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2701 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     2761 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcc
     2821 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2881 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     2941 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3001 gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg agggccgcag
     3061 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     3121 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     3181 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3241 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     3301 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     3361 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3421 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3481 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3541 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3601 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     3661 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     3721 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3781 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3841 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3901 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3961 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4021 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4081 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4141 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4201 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4261 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4321 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4381 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4441 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4501 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4561 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4621 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4681 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4741 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4801 ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg tatcattgca gcactggggc
     4861 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4921 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4981 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5041 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5101 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5161 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5221 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5281 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5341 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5401 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5461 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5521 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5581 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5641 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5701 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5761 ggttcctggc cttttgctgg ccttttgctc acatgtcctg caggcag
//
