LOCUS       CYP168A1_codon_o        6564 bp    DNA        circular     15-MAY-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
FEATURES             Location/Qualifiers
     primer_bind     4983..5002
                     /PCR_conditions="primer
                     sequence:gtgaattg<TAATACGACTCACTATAGGG"
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      1420..2102
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      29..335
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    5002..5024
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(562..1374)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             5070..6428
                     /locus_tag="CYP168A1 His6 codon optimized"
                     /label="CYP168A1 His6 codon optimized"
                     /ApEinfo_label="CYP168A1 His6 codon optimized"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      601 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      661 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      721 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      781 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
      841 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      901 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      961 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     1021 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     1081 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     1141 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     1201 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     1261 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     1321 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     1381 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     1441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2281 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac
     2341 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     2401 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     2461 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     2521 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     2581 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     2641 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     2701 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     2761 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     2821 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
     2881 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
     2941 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
     3001 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
     3061 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
     3121 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc
     3181 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     3241 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     3301 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     3361 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     3421 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     3481 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     3541 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3601 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     3661 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     3721 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     3781 tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     3841 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     3901 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
     3961 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     4021 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     4081 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     4141 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     4201 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     4261 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     4321 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     4381 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     4441 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     4501 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     4561 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
     4621 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
     4681 ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     4741 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     4801 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     4861 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     4921 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa
     4981 attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat
     5041 tttgtttaac tttaagaagg agatataccA TGGACGACGC ATTCAGCGAG GAAGGTAGCG
     5101 CACAGCCTCG TCATGATGCA CAGCGTCCGG CACTGGCACC GCGTAGTGAT GGTTTTGATA
     5161 TTCATACCTA TCATCCGGAT TTTGTGGCAG ATCCGTATCC GCTGCTGCGT CTGATTCGTA
     5221 GCCGTGCACC GGTTTGTCGT GATCAGGCAA GCATTTGGTG GATTAGCCGT TATGCAGATG
     5281 TTAGCGCATG TCTGCGTGAT CGTCGTTTTA GCGCAGATCC GGCACGTCTG GGTGCAGCCG
     5341 GTGTTCGTCA AGGTGGTGCA AGCTGGTTTG GTCATCAGCA GCTGCAGCCT CTGGCACGTT
     5401 TTTATGATAA CTTTATGCTG TTTAACGATG CACCGCGTCA TACCCGTCTG CGTCGTCTGT
     5461 TTGCACCGGC ATTTGGTCCG GATGCAGTTC GTCGTTGGGA AGCACGTATT GAAGTTCTGG
     5521 TTGAAGAACT GCTGGATAGC CTGCTGGAAC GTCGTGAACC TGATCTGCTG CGTGATTTTG
     5581 CAGAACCGCT GACCATTCGT GTTGCAGCAG AACTGTTTGG TTTTCCGCGT GAAGATACCG
     5641 GTCAGCTGCT GCCGTGGGGT CGCGATCTGG CAGCAGGTCT GGACCTGGCA GCAAGCCATG
     5701 GTGATGCAGG TCAGATTAAT CGTAGCGCAG TTGCATTTAG CGATTATCTG CAGCGTCAGG
     5761 CACGCGGTTG GAGTGATGGT AGCAGCCGTC CGCCTAGCGG TGCAGCACCG AGCATTCTGG
     5821 ATGGTGCAGC AATGCTGGAA GCAGGTTTAG GTCTGGAAGA TCTGGTTGCA GCATATGCAA
     5881 TGGTTTTTAT GGCAGCATTT GAAACCACCA TTAGCATGGT TGGTAATGCA ACCCTGGCAC
     5941 TGCTGACCCA TCCGGATCAG CTGGATTTAC TGCGTCGTTG TCCTGAACTG GCAGCCAATG
     6001 CAGTTGAAGA ATTACTGCGT TTTGATGGTG CCGTTCGTGG TGGTGTTCGT TGTACCCTGG
     6061 AAGAAGTTGA AATTGGCGGT CAGCGTATTC CGCCTGGTGA AAAAGTTTGG CTGAGCTTTC
     6121 TGGCAGCGAA TCGTGATCCG GAAATGTTTG CCGCACCGGA TCGTCTGCAA CTGCAGCGTG
     6181 CAAATGCAAA ACAGCATGTT GCCTTTGCAC ATGGTCCGCA TTATTGCCTG GGTGCATATC
     6241 TGGCACGCCT GGAACTGCAG TGTGCCCTGC GTGGTCTGGT TCGTCGTCGC TTTGCACTGG
     6301 CCAGCGAACC GACCGATCTG CGTTGGCGTC GTAGCAGCGT TTTTCGCACC CTGGAACGCC
     6361 TGCCGATTGT TCCGGAAGGC GACGCTCAGA AGACCTGCGA Gctcgagcac caccaccacc
     6421 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     6481 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     6541 tgaaaggagg aactatatcc ggat   
//