LOCUS       pTol2-atp6v0cb:kalta4-attB       11797 bp    DNA     circular SYN 26-JAN-2025
DEFINITION  atp6v0cb-kalta4_B1.
ACCESSION   atp6v0cb-kalta4_B1
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="AmpR"
     promoter        complement(1032..1136)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="AmpR promoter"
     rep_origin      complement(1189..1617)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 ori
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 ori
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; f1
                     ori (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="f1 ori"
     CDS             complement(1622..1777)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacZα
                     (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacZα
                     (fragment) (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="lacZα (fragment)"
     misc_feature    1785..1804
                     /ApEinfo_fwdcolor="#9a9aff"
                     /ApEinfo_revcolor="green"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="T7 promoter"
     CDS             2905..3546
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="51.4"
                     /match_length="39.6"
                     /fragment="True"
                     /other="CDS"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="tyr (fragment)"
     strand          3396..3407
                     /Strand_number_=3
                     /Sheet_number_="C"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on protein similarity"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Beta Strand"
     misc_feature    3609..4239
                     /ApEinfo_label="Tol2 site"
                     /ApEinfo_fwdcolor="#ffbdfe"
                     /ApEinfo_revcolor="#ffbdfe"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Tol2 site"
     promoter        4240..7297
                     /created_by="rreeves"
                     /label="atp6v0cb upstream sequence"
     ORF             7298..7885
                     /created_by="rreeves"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; kalta4
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kalta4
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4 (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="kalta4"
     polyA_signal    7936..8118
                     /ApEinfo_fwdcolor="#ffb78b"
                     /ApEinfo_revcolor="green"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="SV40 poly(A) signal"
     polyA_signal    complement(8179..8311)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.5"
                     /fragment="False"
                     /other="polyA_signal"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="SV40 poly(A) signal"
     ORF             complement(8362..9078)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="EGFP"
     promoter        complement(9079..9977)
                     /ApEinfo_fwdcolor="#ffffbf"
                     /ApEinfo_revcolor="#ffffbf"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="myl7 (cmlc2) promoter"
     misc_feature    9979..10627
                     /ApEinfo_fwdcolor="#f1beff"
                     /ApEinfo_revcolor="#f1beff"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Tol2 site"
     misc_feature    10649..10718
                     /bound_moiety="phage&nbsp;φC31 integrase"
                     /note="attB site for the φC31 integrase (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/10801973""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/10801973"">Grot
                     h et al., 2000</a>)"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="attB"
     rep_origin      complement(11209..11797)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB (reversed)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="ori"
ORIGIN      
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct gaaaacttgg
     1861 atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt atttgtctgc
     1921 atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg actgtgttta
     1981 acagacatta ttacaataat atgtaatagt agtacacact attattatgt aatagtactt
     2041 gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca aaatgactta
     2101 cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta tggacatcgc
     2161 cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc gagtctgagt
     2221 caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga agtccacagc
     2281 ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga
     2341 gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa cttgggctac
     2401 agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat aaagatttta
     2461 atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt ttggatcact
     2521 agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt tggttggttg
     2581 gtagattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     2641 attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga ttcattacag
     2701 aaatgattaa atgcatacat aaaaaactgg gggggggggg gggatacaac aacacactta
     2761 agtcacattt gcctacgtaa agaaaagtaa agaaaatcaa tagctatatt ttacatctcc
     2821 tttttttgct gtcttttaat tagccttgtt ttgctgttat cttgattgaa actgtaactt
     2881 cttcacctgc ttcttttctt tgtaggtttt gccagcccag aaacaggcat ggctgtgcaa
     2941 ggccagagca ccatgcacaa tgcgctgcat gtcttcatga acggctcaat gtcctcagtc
     3001 cagggctcag ccaacgaccc catcttcctc cttcaccatg ctttcattga caggtaacaa
     3061 acacgtcatg acattagact gcacagtttt tgacaaagtt catacaatct gttgtttata
     3121 gctgctacaa ttagtgaagt ttgtgaatgt acttggatga gcagcgaaag atcaattgag
     3181 atcaattgtt agagtttggt tgccctgcag agcaaagaac aaaaaataat ctggtggctt
     3241 tactgcgtga ggttattatt ggtggaatag aaacacaaaa cataattgca tttatttgtt
     3301 taatttttta tcttatctta actttcatct tgcatatttg tttcttacat catttctagc
     3361 atctttgagc gctggctaag aactcatcag cctccccggt ccatctaccc acgtaccaat
     3421 gcaccaattg gccacaatga cggctactac atggtgccat tccttcctct ttataggaat
     3481 ggagactacc tcctgtccaa caatgctctt ggatacgagt acgcctacct gttggaccca
     3541 ggtcattgca caacaccaga aatgccctct gatctgcaaa agacgtgaat aatctgttca
     3601 gacacccata tgcactctgt tccacacagg tcagaggttt gtccaggagt tcttgacaga
     3661 ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt
     3721 tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta
     3781 ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga aagaaaacta
     3841 gagattcttg tttaagcttt taatctcaaa aaacattaaa tgaaatgcat acaaggtttt
     3901 atcctgcttt agaactgttt gtatttaatt atcaaactat aagacagaca atctaatgcc
     3961 agtacacgct actcaaagtt gtaaaacctc agatttaact tcagtagaag ctgattctca
     4021 aaattgttag tgtcaagcct agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa
     4081 agcctctcac aggcagccga tgcgggaaga ggtgtattag tcttgataga gaggctgcaa
     4141 atagcaggaa acgtgagcag agactccctg gtgtctgaaa cacaggccag atgggccatt
     4201 cgtacgctta atttcgtata gggataacag ggtaatgatc taggcacatt cagatggtcg
     4261 ggtcagagtt tggtgtaagc aacatgtaag catggatcca tcctgccttg tatcagcggt
     4321 tcaggctgga ggtggtgtaa tggtgtgggg gagattttct tggcacactt tgggtccatt
     4381 agtaccaatt aagcatcgtg tcaacgccac agactaccct aatgttgttg ctgaccatgt
     4441 ccatcccttt atgaccacag tgtacacatc tcctgatggc tgcttccagc aggataacgc
     4501 accatgttat aaagtacaaa tcatctcaga ctggtttctt gaacatgaca acgagttcac
     4561 tgtactcaaa tggcctccac agtcatcaga gctcaatcca atagagcact tttgggatgt
     4621 ggttgaatgg gagattggca tcatgaatgt gcagccgaca aatctgcagc aactgtgtga
     4681 tgctatcatg ttaatatgga gcaaaatctc tgaggaatat ttccagtatc ttgttaaatc
     4741 ttgccacaaa ggattaaagt agttctgaat gcaaaatggg gcccaacccg gtactagtaa
     4801 ggtgtaccta ataaagtggc cagtgagtgt atgcataaat tgattcatgt gccaatatct
     4861 aaacatcatt aaatcgagaa gcgttttcta cacaagcata aaatattggt cccactttat
     4921 attaagtggc cttaactaat atgtacttac ataggaatta atagtttgtt acaatgcact
     4981 tgttgtgtaa atacatgtat ttactgtgta cttatacttg attaaataca tgtatgtaat
     5041 tacatctata attaactttt gtaattacat ttgtaaatac actgttgacc atcccttaca
     5101 ccttaaccta cccttaaacc tacccatgcc aacaaacctg ttcataaccc aacctctatt
     5161 ccaactcaaa agcaccagaa gtgttctcaa atacattata aacacagtaa gtacattgta
     5221 tttatttttt tatgtaagta catagtagtt agggacactt aatataaagt gggaccaaaa
     5281 tattttcttg ttttaaaaaa taatttaaat tatgaatgat ttaataatct gccagtgggg
     5341 taagcaaaat aatcgtatgt caaatggaaa aactcaactt tttttgcatg ctgtaaggaa
     5401 aaaactcact taattttgac gtattatttc ttaaaaccca accttgtctg gaaaatgctg
     5461 ctcgatttag ggatttttag atatttggac cagaaactac actaaatatc taagtaagag
     5521 aagcatttat tgcagtgtac tcaattgaga aaaatccctg ttaatacatc tcttcaatat
     5581 taacgtcata tcaaaactat agttctatta ctgtaattta atataataca ttatttatga
     5641 aacacaaaac aacctatggt ttcccagtac tgggttgcgg ctggtagggc atccactgtg
     5701 taaaacatgc tggataagtt ggcggttcat tccactgtgg cgacccctga tgaataaagg
     5761 gactaagcag aaggaaaatt aatgaagaca ataaaagttg accaagaaca caaagtaaaa
     5821 aggctctcag attaatcagt aaggtcagct ttacaactgt accctgcact aaaatctgag
     5881 aaattctcaa tcacacgcgc gcctgtcatt tgacctttat gatgcagatt ttccctttgg
     5941 ctaaatgaga ccagactaat tgatctctcc tcgtgtgtcc tgaacaacca tgtctaccat
     6001 gctgacataa acaccgcata attactcaca attatttgca ttaaaaatac acttttgttt
     6061 atttgatgca ttattgactg ggaataaaat atgcattaac caaccatcta ataaacaccc
     6121 cacctggctt tatctcatgt ttattcatat ttttttgtga atattcttaa attcttgctg
     6181 acatgagccg gtgggcgtgg cttgcgcttg acgtcactgg tcagaatgac cttcatctcg
     6241 ggaatcctca tccgctgcat cacactttac tgcgtggctg acataatatc tcttatatga
     6301 taaagaagct acattataat gtgatgttga tcatctgagg cctatattag atacgttaga
     6361 taacgtttga aagaccaaat gagttttctt aatgatatat catcgaatgc agtgtgatag
     6421 tataatcatg tacgccctta caaacagtac ttcatgttaa aatgctgaca ctatggaaaa
     6481 atattaaata aatgtattat acatacaatt cggtattaat attatacata aatggcgcat
     6541 gttgatatat tttagtgcac acgttcttaa tatatctagg aaataattta ttataagtta
     6601 cataatgctt aaaaccttct aatctcttta aaatatattt aacacatcga tttctcccat
     6661 tattttacat tatttatgaa ggaaaacagt gttatatgtg tatctggtgt agaatatacg
     6721 tgctgcaatg atttttagag catgattaaa tgatttaaat gaaagtaata cgaaactagt
     6781 atgttcacac ccagattaca aacgcacgat gttttattct aagggtttaa acatcttcag
     6841 tattaaaggg aaagcccgca gcggagcgca ctaaactcgc tacaaatggg ggagccccga
     6901 aatcaaatac cctctgaaat actgttagca ttgcacgtaa atgcttcatt atatttgttt
     6961 tacaatgttt atccatgtca tgttcgttcc atgactcgaa tagaagcagt ataccagcgc
     7021 atccctgttt ttctcgatct cctccccctt tcaggagtcg gatgggattt tcttcacggt
     7081 gtgtcaggtg acgttttcgg ccattttgta ccgcagattc atcctacagg atccgttacg
     7141 agcagtttcc atcatttcac cggggtttat taaaaccatt ggtccatagc gaaagcttta
     7201 ccctaataga caaatacccg ttagtctata cgccttcgct cgtgtccagc agtgtaagtt
     7261 cggtgttact gggttttgag gtgaaattag aggaataatg aaactgctct catccatcga
     7321 gcaagcctgc gacatttgtc ggcttaagaa gctgaaatgc tccaaggaaa agccgaaatg
     7381 tgccaaatgc ctgaagaaca attgggaatg tcgttactct cccaaaacca agcgaagtcc
     7441 actcacaagg gctcatctga ccgaagtgga gagcaggcta gagagactgg aacaactctt
     7501 tttgctcatc ttccctagag aggaccttga catgatcctc aagatggatt ctctccagga
     7561 tattaaagcc cttttgactg gcttattcgt ccaggacaat gtgaacaaag acgctgtgac
     7621 agaccgattg gcaagtgtcg agaccgatat gcctctgaca ctgagacagc acagaatcag
     7681 cgctacttcc tcaagcgaag agtcttctaa caagggacag agacagctga ctgtttcgag
     7741 caggtcgacc ccgtccccgg ccgacgccct ggacgacggc gacctggaca tgctgcctgc
     7801 tgatgctctc gatgatttcg atctggatat gctcccggcc gacgccctgg acgactacga
     7861 cctggacatc ctcccgggta actaacaaac tcgagaaagc tagcaaacca tggtacgatg
     7921 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     7981 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     8041 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     8101 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     8161 atcctctaga tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag
     8221 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8281 actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga
     8341 ggctcgagag gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
     8401 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     8461 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     8521 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     8581 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     8641 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     8701 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     8761 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     8821 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     8881 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     8941 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     9001 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     9061 ctcgcccttg ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac
     9121 tggcttacta atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt
     9181 ctgcagtgtt tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc
     9241 tgataactgc acaagtgctc agattccagc agggtggaaa atgagataaa gtgtgcagat
     9301 ggggaggggg acgtgaatga gagatttgag ggatgaaaag gatggatgaa cgcattgaaa
     9361 atagcccctt tcacaaaata ataccagtaa attgtcatat aattaatgac cagaccttta
     9421 cctgtaaatg atgtgatgtt catatacaga agaatgccaa caatttacag atgattttac
     9481 aaattcttaa ttttcctttt ctaaatggat ttgttcacac attatctctt caaagcaatt
     9541 gaattttctg gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa tttaatgtga
     9601 catggaatga agcaaacagc agcctaagac aggatggcag gaaaaatgtc acatgtttaa
     9661 aataagagtg aaaccaacga cctggactta tagagactgt gtgtccttag gcaaagctct
     9721 tgtacaaaca gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg ttaaatgtgt
     9781 gcttggatgt atcataaaaa agaatcggtt ctctgtattt gacattttct gtaatttatc
     9841 taaagttaca actggctatg cctgattttt atcactattg aatcaatcat tttaaagaat
     9901 gttaagtctg ctgatttgga gagctcattt acatttattt tgaatgtctc ttatttaaca
     9961 caactgattt aagctttaga tctaatctga tcattcaggc gcgccaacac gtgaatacca
    10021 cgcgaggcct tagggataac agggtaatac gcgtgatctg cgaagatacg gccacgggtg
    10081 ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac
    10141 ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg
    10201 ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc
    10261 tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt
    10321 aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt
    10381 gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact
    10441 tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca
    10501 agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa
    10561 gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag atccactagt
    10621 tctagagctc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
    10681 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatctga tgaacgggcc
    10741 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
    10801 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10861 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10921 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    11041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    11101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    11161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    11221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    11281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    11341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    11461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11761 caagcagcag attacgcgca gaaaaaaagg atctcaa
//
