LOCUS       pTol2-sncb:kalta4-attB       11794 bp    DNA     circular SYN 26-JAN-2025
DEFINITION  Sncb-kalta4_colony_F8.
ACCESSION   Sncb-kalta4_colony_F8
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="AmpR"
     promoter        complement(1032..1136)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="AmpR promoter"
     rep_origin      complement(1189..1617)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 ori
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 ori
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; f1
                     ori (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; f1
                     ori (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="f1 ori"
     CDS             complement(1622..1777)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacZα
                     (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacZα
                     (fragment) (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="lacZα (fragment)"
     misc_feature    1785..1804
                     /ApEinfo_fwdcolor="#9a9aff"
                     /ApEinfo_revcolor="green"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="T7 promoter"
     CDS             2906..3547
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="51.4"
                     /match_length="39.6"
                     /fragment="True"
                     /other="CDS"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="tyr (fragment)"
     strand          3397..3408
                     /Strand_number_=3
                     /Sheet_number_="C"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on protein similarity"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Beta Strand"
     misc_feature    3610..4240
                     /ApEinfo_label="Tol2 site"
                     /ApEinfo_fwdcolor="#ffbdfe"
                     /ApEinfo_revcolor="#ffbdfe"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Tol2 site"
     promoter        4241..7294
                     /created_by="rreeves"
                     /label="sncb upstream sequence"
     ORF             7295..7882
                     /created_by="rreeves"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; kalta4
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kalta4
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4 (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4 (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="kalta4"
     polyA_signal    7933..8115
                     /ApEinfo_fwdcolor="#ffb78b"
                     /ApEinfo_revcolor="green"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="SV40 poly(A) signal"
     polyA_signal    complement(8176..8308)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.5"
                     /fragment="False"
                     /other="polyA_signal"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="SV40 poly(A) signal"
     ORF             complement(8359..9075)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="EGFP"
     promoter        complement(9076..9974)
                     /ApEinfo_fwdcolor="#ffffbf"
                     /ApEinfo_revcolor="#ffffbf"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="myl7 (cmlc2) promoter"
     misc_feature    9976..10624
                     /ApEinfo_fwdcolor="#f1beff"
                     /ApEinfo_revcolor="#f1beff"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Tol2 site"
     misc_feature    10646..10715
                     /bound_moiety="phage&nbsp;φC31 integrase"
                     /note="attB site for the φC31 integrase (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/10801973""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/10801973"">Grot
                     h et al., 2000</a>)"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="attB"
     rep_origin      complement(11206..11794)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /X-Transferred_From="pTol2-elavl3:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="ori"
ORIGIN      
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct gaaaacttgg
     1861 atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt atttgtctgc
     1921 atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg actgtgttta
     1981 acagacatta ttacaataat atgtaatagt agtacacact attattatgt aatagtactt
     2041 gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca aaatgactta
     2101 cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta tggacatcgc
     2161 cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc gagtctgagt
     2221 caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga agtccacagc
     2281 ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga
     2341 gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa cttgggctac
     2401 agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat aaagatttta
     2461 atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt ttggatcact
     2521 agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt tggttggttg
     2581 gtagattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     2641 attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga ttcattacag
     2701 aaatgattaa atgcatacat aaaaaactgg gggggggggg ggggatacaa caacacactt
     2761 aagtcacatt tgcctacgta aagaaaagta aagaaaatca atagctatat tttacatctc
     2821 ctttttttgc tgtcttttaa ttagccttgt tttgctgtta tcttgattga aactgtaact
     2881 tcttcacctg cttcttttct ttgtaggttt tgccagccca gaaacaggca tggctgtgca
     2941 aggccagagc accatgcaca atgcgctgca tgtcttcatg aacggctcaa tgtcctcagt
     3001 ccagggctca gccaacgacc ccatcttcct ccttcaccat gctttcattg acaggtaaca
     3061 aacacgtcat gacattagac tgcacagttt ttgacaaagt tcatacaatc tgttgtttat
     3121 agctgctaca attagtgaag tttgtgaatg tacttggatg agcagcgaaa gatcaattga
     3181 gatcaattgt tagagtttgg ttgccctgca gagcaaagaa caaaaaataa tctggtggct
     3241 ttactgcgtg aggttattat tggtggaata gaaacacaaa acataattgc atttatttgt
     3301 ttaatttttt atcttatctt aactttcatc ttgcatattt gtttcttaca tcatttctag
     3361 catctttgag cgctggctaa gaactcatca gcctccccgg tccatctacc cacgtaccaa
     3421 tgcaccaatt ggccacaatg acggctacta catggtgcca ttccttcctc tttataggaa
     3481 tggagactac ctcctgtcca acaatgctct tggatacgag tacgcctacc tgttggaccc
     3541 aggtcattgc acaacaccag aaatgccctc tgatctgcaa aagacgtgaa taatctgttc
     3601 agacacccat atgcactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
     3661 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
     3721 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
     3781 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
     3841 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     3901 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     3961 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     4021 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     4081 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     4141 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccat
     4201 tcgtacgctt aatttcgtat agggataaca gggtaatgat ctagggtatt gcttgatcaa
     4261 aacaacgtct tacgcgaaaa cagagacatt aggacttatt gaagccggac actgttaaat
     4321 cagcatctgc tcccgatctt aatgagcctt cagagaaaat cgatcggtca gtcgaggctc
     4381 ttgtattgtg tttgagaggc agatgtcacg attttcagca gatggacaga ggacacacac
     4441 acgcattgat cccacagaga tatgctggag cagagcgcac ctactgttca ctctcccttt
     4501 aaacatcgtg acgagaatct actataccgt ccacgctttt agacttgcag cgtattcgta
     4561 gtagatggcc tgcgatctca aacacatgcg gatagactac agtgaagcca atgaggatag
     4621 tttctcaaca ttatatacaa tactgcctgt ataaatggat atcctcttgg atggatggat
     4681 ggatggatgg atggatggat ggatggatgg atggatagat agatagatag atagatagat
     4741 agatagatag atagatagat agatagatag atagatagat agatagatag atggatggat
     4801 ggatggatgg atggatggat ggatggatgg atggatggat ggatggatgg atggatggat
     4861 agatagatct aacaaaccat ttatctagct atttacttac tagccaactg attaagatgg
     4921 ctgaaaacct gtattccttt gacttttata gtatttgctt ttcctactat gggagtcaat
     4981 ggttacaggt ttcgaacatt tttctaaata acttaatttg tgtttaacag aagaagaaaa
     5041 aaccccataa aggtttgaaa cttaaaacca cttcagcgaa agtaaatagt gagaatattt
     5101 tcatttttgg gtgaactgtc cccatatagc aaggtgttag ttataaaaac actatgggat
     5161 gagataacag acagacagac agacagacag acagacagac agtcagatag atagatagat
     5221 agatagatag atagatagat agatagatag atagatagat agatagatag atagatagat
     5281 agatagatag atagatagat agatagatct aacaaactat ttaactttga agatggctga
     5341 aaagctgtaa ccattgactt ccatggtatt ttttttctaa ctatggaagt caatggttac
     5401 aggttttgaa ttttttttta ataattcatt ttgtgttcaa cagaaaaaaa ataaatccat
     5461 aaaggtttga aagttcttga gggagagtac attttcatct ttgcgtgaac tgtcctttaa
     5521 tatttattct aatagtaagg tgtttgttat aaaatacgtg actacagaca gacagacaga
     5581 cagacagaca gacagataga cagatagata gatagataga tagatagata gatagataga
     5641 tagatagata gatagataga tagatagata gatagataga tagatagata gatagataga
     5701 tagatagata actatttaac gttgaagatg gctgaaaacc tgtaaccaat gacttttata
     5761 gtatttgctt ttccaattcc tactatggaa agtcaacggt tacaggtttc aaacacttct
     5821 aaataactta ttttgtgttc agcagaaaaa aataaacata taaaggtttg aaattgcata
     5881 agcgagagta aataataagt ttccatttaa ggtgaactgt ccctttaata tagcaaggtg
     5941 ttataaaata ctatgtgata ggatatgata cggatagata gatagataga tagatagata
     6001 gatagataga tagatagata gatagataga tagatagata gatagataga tagatagata
     6061 gatagataga tagatagata gatagataga tagatagata gatagataga tagatagata
     6121 gatagataga tctaaacatg ctcaataatt atctatacac taatagtcta tacactaaac
     6181 gcagcggtac atacttcata tgcaatccga ttatagttca gtattattaa acacagcgaa
     6241 tgtaatgaca gtcagtcgca cgtgcacaca gtgagtaaac gcagatgatc aaatcttctg
     6301 tgatgtgtgt gtgtgtctcc tcatccctca gctgatctcc gcggtgtccg tcctctgtaa
     6361 tgtccgctca ggtgcgcatc cgtgtcaggg agctcaggcc attagagccg ctctctgact
     6421 gactgacacc cccgtgatga tcaataccgc atcctcgcct cctgattggc tgagcgcatc
     6481 atccatagga aggcccacct cgagagcgta gtgtaacatt tccttccacg atatttcaat
     6541 ctagcacgca attgaagcac aaggacgaag aaggtgcttt aaaaaggtag gctttcgatt
     6601 gcgttgatca cagaaaagtg cgtgtttgta gggacgcgaa cggagatcgt gtgaaatagc
     6661 tcgtgtgcat gtgtgtgtgt gaggagactc gtagagggag gaaaattcat cgatgcaatc
     6721 gtatttgaat tgaatgcatg ttgaatatcc aatgcaacct gatgattaaa tgaatactcg
     6781 ctgtgtttat tttctgagtc gtgtcgtgtg cgggacgttg gagaggtgac accatgttgt
     6841 aactgatcag tgtgtaagat ccatcatctt caacgtgcat tgtttaacat cacaccaatg
     6901 cattcgagat ttattaaatg cttaaaatcg acattaatgc tcagcattat tatatacaaa
     6961 ccctacagca gtggctaagt gtaaaataag agctttgagt aataattaat gagttggtga
     7021 agatctcatg cacacctcct gctgttaaag ctgaaaaata gcgtcagctg atggtgtgaa
     7081 tcttctcatc tccttgtgtt tgcatgtatg ggcgttttat cttctgggaa attagactcc
     7141 ttattgttcg actgacagcc tgctattaca cacacacaca cacacacaca cacacacaca
     7201 cacacacaca cacacacaca gatgctctca attccaaatt aacccacatt tgtcctatgc
     7261 attgcaactc ctttttttat gcagaggggc caagatgaaa ctgctctcat ccatcgagca
     7321 agcctgcgac atttgtcggc ttaagaagct gaaatgctcc aaggaaaagc cgaaatgtgc
     7381 caaatgcctg aagaacaatt gggaatgtcg ttactctccc aaaaccaagc gaagtccact
     7441 cacaagggct catctgaccg aagtggagag caggctagag agactggaac aactcttttt
     7501 gctcatcttc cctagagagg accttgacat gatcctcaag atggattctc tccaggatat
     7561 taaagccctt ttgactggct tattcgtcca ggacaatgtg aacaaagacg ctgtgacaga
     7621 ccgattggca agtgtcgaga ccgatatgcc tctgacactg agacagcaca gaatcagcgc
     7681 tacttcctca agcgaagagt cttctaacaa gggacagaga cagctgactg tttcgagcag
     7741 gtcgaccccg tccccggccg acgccctgga cgacggcgac ctggacatgc tgcctgctga
     7801 tgctctcgat gatttcgatc tggatatgct cccggccgac gccctggacg actacgacct
     7861 ggacatcctc ccgggtaact aacaaactcg agaaagctag caaaccatgg tacgatgatg
     7921 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     7981 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     8041 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     8101 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     8161 ctctagatca gatctcttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8221 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8281 catcaatgta tcttatcatg tctggatcta cgtaatacga ctcactatag ttctagaggc
     8341 tcgagagggg ccgctttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
     8401 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
     8461 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
     8521 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     8581 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     8641 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     8701 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     8761 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     8821 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
     8881 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     8941 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     9001 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     9061 gcccttgctc accatggtca ctgtctgctt tgctgttggt ctgggctcct gggtcactgg
     9121 cttactaatg gagtctttat gtatgaggac tcttatcaat tgttcttcta taaaggtctg
     9181 cagtgtttct gttcgtcccc tacatggaca cccagagcct cctaaataca ggagccctga
     9241 taactgcaca agtgctcaga ttccagcagg gtggaaaatg agataaagtg tgcagatggg
     9301 gagggggacg tgaatgagag atttgaggga tgaaaaggat ggatgaacgc attgaaaata
     9361 gcccctttca caaaataata ccagtaaatt gtcatataat taatgaccag acctttacct
     9421 gtaaatgatg tgatgttcat atacagaaga atgccaacaa tttacagatg attttacaaa
     9481 ttcttaattt tccttttcta aatggatttg ttcacacatt atctcttcaa agcaattgaa
     9541 ttttctggaa aagattggct gtgtgtgtaa aaggtgctac tgaggaattt aatgtgacat
     9601 ggaatgaagc aaacagcagc ctaagacagg atggcaggaa aaatgtcaca tgtttaaaat
     9661 aagagtgaaa ccaacgacct ggacttatag agactgtgtg tccttaggca aagctcttgt
     9721 acaaacagtg cttgtgactg tgaaattcgg ggtttgcctg gattgtgtta aatgtgtgct
     9781 tggatgtatc ataaaaaaga atcggttctc tgtatttgac attttctgta atttatctaa
     9841 agttacaact ggctatgcct gatttttatc actattgaat caatcatttt aaagaatgtt
     9901 aagtctgctg atttggagag ctcatttaca tttattttga atgtctctta tttaacacaa
     9961 ctgatttaag ctttagatct aatctgatca ttcaggcgcg ccaacacgtg aataccacgc
    10021 gaggccttag ggataacagg gtaatacgcg tgatctgcga agatacggcc acgggtgctc
    10081 ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc
    10141 ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg
    10201 tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga
    10261 ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa
    10321 ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat
    10381 ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt
    10441 ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt
    10501 aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta
    10561 ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct
    10621 agagctcgac gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct
    10681 tgggctcccc gggcgcgtac tccacctcac ccatctggtc catctgatga acgggccacc
    10741 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
    10801 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10861 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10921 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10981 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    11041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    11101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    11161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    11221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    11281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    11341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11401 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11641 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11761 gcagcagatt acgcgcagaa aaaaaggatc tcaa
//
