LOCUS       pTol2-stmn1b:kalta4-attB       13061 bp    DNA     circular SYN 26-JAN-2025
DEFINITION  stmn1b-kalta4_G6.
ACCESSION   stmn1b-kalta4_G6
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="AmpR"
     promoter        complement(1032..1136)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="AmpR promoter"
     rep_origin      complement(1189..1617)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 ori
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; f1
                     ori (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; f1
                     ori (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 ori
                     (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; f1
                     ori (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; f1
                     ori (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; f1
                     ori (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="f1 ori"
     CDS             complement(1622..1777)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacZα
                     (fragment) (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacZα
                     (fragment) (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="lacZα (fragment)"
     misc_feature    1785..1804
                     /ApEinfo_fwdcolor="#9a9aff"
                     /ApEinfo_revcolor="green"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="T7 promoter"
     CDS             2907..3548
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="51.4"
                     /match_length="39.6"
                     /fragment="True"
                     /other="CDS"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tyr (fragment) (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     tyr (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="tyr (fragment)"
     strand          3398..3409
                     /Strand_number_=3
                     /Sheet_number_="C"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on protein similarity"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Beta Strand (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Beta Strand (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Beta Strand"
     misc_feature    3611..4241
                     /ApEinfo_label="Tol2 site"
                     /ApEinfo_fwdcolor="#ffbdfe"
                     /ApEinfo_revcolor="#ffbdfe"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Tol2 site"
     promoter        4242..8561
                     /created_by="rreeves"
                     /label="stmn1b upstream sequence"
     ORF             8562..9149
                     /created_by="rreeves"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; kalta4
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4 (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4 (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kalta4
                     (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4 (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4 (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4 (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="kalta4"
     polyA_signal    9200..9382
                     /ApEinfo_fwdcolor="#ffb78b"
                     /ApEinfo_revcolor="green"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="SV40 poly(A) signal"
     polyA_signal    complement(9443..9575)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.5"
                     /fragment="False"
                     /other="polyA_signal"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="SV40 poly(A) signal"
     ORF             complement(9626..10342)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EGFP (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EGFP (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="EGFP"
     promoter        complement(10343..11241)
                     /ApEinfo_fwdcolor="#ffffbf"
                     /ApEinfo_revcolor="#ffffbf"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     myl7 (cmlc2) promoter (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     myl7 (cmlc2) promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="myl7 (cmlc2) promoter"
     misc_feature    11243..11891
                     /ApEinfo_fwdcolor="#f1beff"
                     /ApEinfo_revcolor="#f1beff"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Tol2 site"
     misc_feature    11913..11982
                     /bound_moiety="phage&nbsp;φC31 integrase"
                     /note="attB site for the φC31 integrase (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/10801973""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/10801973"">Grot
                     h et al., 2000</a>)"
                     /modified_by="rreeves"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="attB"
     rep_origin      complement(12473..13061)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /X-Transferred_From="pTol2-rtn1a:kalta4-attB"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ori (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     ori (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-sncb:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori (<a
                     href=""urn:local:.:18-iqmbz06"">pTol2-sncb:kalta4-attB</a>
                     )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a
                     href=""urn:local:.:1f-iqmc1w6"">pTol2-rtn1a:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a
                     href=""urn:local:.:q-iqmbq7o"">pTol2-atp6v0cb:kalta4-attB<
                     /a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="ori"
ORIGIN      
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct gaaaacttgg
     1861 atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt atttgtctgc
     1921 atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg actgtgttta
     1981 acagacatta ttacaataat atgtaatagt agtacacact attattatgt aatagtactt
     2041 gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca aaatgactta
     2101 cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta tggacatcgc
     2161 cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc gagtctgagt
     2221 caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga agtccacagc
     2281 ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga
     2341 gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa cttgggctac
     2401 agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat aaagatttta
     2461 atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt ttggatcact
     2521 agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt tggttggttg
     2581 gtagattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     2641 attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga ttcattacag
     2701 aaatgattaa atgcatacat aaaaaactgg gggggggggg gggggataca acaacacact
     2761 taagtcacat ttgcctacgt aaagaaaagt aaagaaaatc aatagctata ttttacatct
     2821 cctttttttg ctgtctttta attagccttg ttttgctgtt atcttgattg aaactgtaac
     2881 ttcttcacct gcttcttttc tttgtaggtt ttgccagccc agaaacaggc atggctgtgc
     2941 aaggccagag caccatgcac aatgcgctgc atgtcttcat gaacggctca atgtcctcag
     3001 tccagggctc agccaacgac cccatcttcc tccttcacca tgctttcatt gacaggtaac
     3061 aaacacgtca tgacattaga ctgcacagtt tttgacaaag ttcatacaat ctgttgttta
     3121 tagctgctac aattagtgaa gtttgtgaat gtacttggat gagcagcgaa agatcaattg
     3181 agatcaattg ttagagtttg gttgccctgc agagcaaaga acaaaaaata atctggtggc
     3241 tttactgcgt gaggttatta ttggtggaat agaaacacaa aacataattg catttatttg
     3301 tttaattttt tatcttatct taactttcat cttgcatatt tgtttcttac atcatttcta
     3361 gcatctttga gcgctggcta agaactcatc agcctccccg gtccatctac ccacgtacca
     3421 atgcaccaat tggccacaat gacggctact acatggtgcc attccttcct ctttatagga
     3481 atggagacta cctcctgtcc aacaatgctc ttggatacga gtacgcctac ctgttggacc
     3541 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga ataatctgtt
     3601 cagacaccca tatgcactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
     3661 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
     3721 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
     3781 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
     3841 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
     3901 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
     3961 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
     4021 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
     4081 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
     4141 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggcca
     4201 ttcgtacgct taatttcgta tagggataac agggtaatga tctaggttgc cctcagtgat
     4261 agcagccaaa aaatgtccca acagggggtt tctcagctct ctttgtgtct ttgtgtaaga
     4321 caccagagac tcgagacagg gcgaggcatg gaagcaccat ctgctccccc tttccctggg
     4381 cagcttgctt taaaatgtcc cccctcaaac gggatgaatg ggatccgggg tggggcagga
     4441 ggaggctggc agagcatgat acagataaaa aaaattgtga tctttatgca aaataatcaa
     4501 gcaatacaat caacaccatc aaaaggatag atgatatgat agatgaaatg ataaattaca
     4561 tttactcact atctattgat cctcaagtgg ttaaaccttt ataagtttca tacttcaaaa
     4621 aaataaaaca ctttgaagaa agctgaaaag ctgtaagcat tgacttcctc agtaaacaaa
     4681 tatcatggaa gtcagtggtt acaggtgttc aacattttcc aaaatatctt attttgtgtt
     4741 caacttcaaa agaaacttat aaaggtttga aacaagtaaa atgatgacag aattttcatt
     4801 tttgactaaa ctatcccttt aaataatgta ttttcttcac tttggtgaag aatttggagc
     4861 taaggaatga tcacaccaca atattaatgc atttaaagag tcaaaacaaa cgactccaac
     4921 agcatttagt gacacaggaa taaacagcag ccacaagact gtaaaatcca gtcagcttga
     4981 aatcatcagc tctgccattt agctaaagtt catacaatga tactgaggca cagagaagaa
     5041 cagaagcaaa acactcacta atgccaagtg aactgttaat tgcttcgcac cgaagacaat
     5101 ttgtaaagca ggtcaccggc gtgtcgctgt tttgttctaa gaacttattg actgtatagt
     5161 catctctgac atgtcgcgag atgactaata ccaggggtga ggtcaggact cactttttcc
     5221 taataaagct tcatgtgatt atagtttgta agctggtttt tgagaaaaaa taaatgtgct
     5281 tatgacaagt tgtcatgtag gtaaatcact ttacattata gccttacaaa caaagctcta
     5341 acataatcag aactgaccca actgtgcatt atgtgagcgt caaattcaag ctcaagttga
     5401 tatctttcac tacacagcct actaataatg ttggtgaata aaacaaaaca aaatgaactt
     5461 gaagctactt aagtgtgatg tatgtgacta aacaaaaatg cttattgtgt tgaacaggtc
     5521 tcccaacttg actcagtctc catctgctac tggtcagttt aactgaaagc cccacccaaa
     5581 acccacacca ttggttaagc cagtgtcact gtgctggtca aatcccaatt ctaaaattgt
     5641 atgaaaaatg ttttatagcc aatgtcttat atacaaaagc tatagaggtg agttttcact
     5701 tttaaccaca ataaacacaa tatttgtgca ctttaaagtt aaagtaacat ttaaacattt
     5761 cttgacatga ccttttatca tatcattttt tagttaatct gccctaaaaa catcatgtaa
     5821 acaaaaactg acttaaaact tatgctaata cactttagct ttaaatgagc aatgtctttt
     5881 taaacatagg ctacccaaca gctacccgaa aatattagtt agcataaaca gtacattgtc
     5941 actggataaa acaatgacaa acggcaacgc tattaaacat ttcactacat aaatgatgac
     6001 atattaactg ttaaaacaga gcagtagttt ttacacatat tttcatacaa agggcatttt
     6061 tgtcagcaat cgtataattt gatgcttctc gtcatatata ggttaactgc atttctattt
     6121 agaataatat agtagaagca tacttcctaa gtgtgaagag ggtcatacta atggggtata
     6181 tccacactga cttttaattt cagtcatcca gccacatggc ttccggttaa ttgtcatccc
     6241 atacaaaaaa cgttgatctt ctctgcctgg ctgagatacg atataaagtg agtatcaaag
     6301 aaaaagcact acattaaagt atatttttcc acgtcaagtc tttataatat ggaaattaat
     6361 tttgtgccag tattttcaat ggaatttctg cgctagcctg ttgctaatga cgtcgctagc
     6421 atttacacca ttgttttaca gtctttcgtc tctttttagc tttaacaatt aaatgaatga
     6481 ttaggtctat ttaagtcatt tgaactacat tacaacattg atgctgtaaa aggaattaaa
     6541 ttgaaaatag tcacataaag ctttaatcgg aagttcatca ttggctgaaa aacactagct
     6601 gtgcatttag tacatttaaa agcagtttat gttaggacaa tgaacttatt ttaccgttca
     6661 aacgataccg tactacattt cccagaattc tgctctcgac aatcattggt ctcatccaaa
     6721 agtgacgtaa tacattttgg tcctcttttg cgcgaagcga accgttgatg ctgaggcaga
     6781 gaggaatctg cgcgcgggca actccttccc tgatttccac ttcttctgaa ggtgggtgtt
     6841 tttttttgtt gttgttgtca aatatcacta tttttgcagc gcatatccca tcccttcctt
     6901 acgtgtcaaa cagcgaatgc atttaaagag taaactttta aattcttaca attataatgt
     6961 tctgccacac cgttatttac ctcctttttt ggggagggga tgtacaacgt taggtaacgt
     7021 taatggacaa gggcttcgag aatattcagt tcggttggag agtttcagta actaacgtta
     7081 agtctgtgaa tttaaaaata gcacgcgcag tcgagtaaca gctcgtagcg ggaccattaa
     7141 tgtgtttatc gtcgttatat ctggagggaa ttggtcgaga ttagccagct ggctgtcgtt
     7201 tcatcgagtt ttgcaggttt atttttgact gtttggtgtt gcaaaaacgt gctttattgt
     7261 catccacaga cttatcgtct gtttcactta aatcttaaca agcatacaac acaatgtgac
     7321 aggtgatcta atggctctcg acgtcttttg tcttcgttat ttgtgcattc gccaactatg
     7381 ccgtcatatt atattattta tattcacatg cttcaataaa atcgatcagt aatggcatag
     7441 cacgctgcat ttggcatacc aacactgatt attctgtaaa aaaggcagtg cagttgtgtc
     7501 tggtttgaaa cggtagagat ttgggtgtga tctggtgtgc tggaaacggg tgattcagcc
     7561 atggctttat tagtaaatgt tgcatttcaa atgtgcatat tctaaaacat tattaagctc
     7621 ttggtcatca tgcaataaag tgttgtatgt aatattatgt atttttgcat gctgttataa
     7681 tgggtgtatc tcggccatct attgtctgca gatgagatct ttcatccctc cggataatcg
     7741 ctagtgttgg atgtttattt tcagccatcg agctatgaat tgttcacagg tcaaattttg
     7801 agatgacaag ttcgtctaat aataaatatt tgacgattct ccagggggca ttagctcgag
     7861 gatcaatgct ctgttgtctt tgttcgcttc ttacacaata tctcctttgt tttaatctct
     7921 caatggccaa ttgaatcacg ttacattata aaaaaagagg cagcgttgtt tgctctacat
     7981 ttcaccagag cggtcagcct gtgttgtata ttttaattga actattatcg tttaattaga
     8041 cgatgatgac agcacgtctg aatggaagga ttcggttttt ctgctggcag tgactcgtct
     8101 ttttttcttg gctttcagca aaaggaggat atattggcat gaattaggct gtatttttgt
     8161 tgcattttta acgagtattg cacagtcttg cctatttcct taccgtaaaa caattcactt
     8221 agtaaattat gcatgtctat tgtggcgacg cccctccctt aagcacgtgt acattattac
     8281 attatataaa catcgtttcc tatcttgtaa aaatgttata gtaggcagca gggactgact
     8341 ccagtcatca tttttcataa atgtctacct taaacatttt tcattcattg attgaacact
     8401 gctgtataga tgtctgttta caatacattg tggttaaata tggttgagtg aaaagattgc
     8461 ctagacattg ttgttaacag caaaacgttt ccatttttct aacatcatta ttatttacaa
     8521 tcatttcgca ggtgtctgaa accgtcgaag tagaaagtgc aatgaaactg ctctcatcca
     8581 tcgagcaagc ctgcgacatt tgtcggctta agaagctgaa atgctccaag gaaaagccga
     8641 aatgtgccaa atgcctgaag aacaattggg aatgtcgtta ctctcccaaa accaagcgaa
     8701 gtccactcac aagggctcat ctgaccgaag tggagagcag gctagagaga ctggaacaac
     8761 tctttttgct catcttccct agagaggacc ttgacatgat cctcaagatg gattctctcc
     8821 aggatattaa agcccttttg actggcttat tcgtccagga caatgtgaac aaagacgctg
     8881 tgacagaccg attggcaagt gtcgagaccg atatgcctct gacactgaga cagcacagaa
     8941 tcagcgctac ttcctcaagc gaagagtctt ctaacaaggg acagagacag ctgactgttt
     9001 cgagcaggtc gaccccgtcc ccggccgacg ccctggacga cggcgacctg gacatgctgc
     9061 ctgctgatgc tctcgatgat ttcgatctgg atatgctccc ggccgacgcc ctggacgact
     9121 acgacctgga catcctcccg ggtaactaac aaactcgaga aagctagcaa accatggtac
     9181 gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     9241 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     9301 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     9361 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     9421 tatgatcctc tagatcagat ctcttgttta ttgcagctta taatggttac aaataaagca
     9481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9541 ccaaactcat caatgtatct tatcatgtct ggatctacgt aatacgactc actatagttc
     9601 tagaggctcg agaggggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg
     9661 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     9721 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     9781 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     9841 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     9901 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     9961 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
    10021 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
    10081 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
    10141 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
    10201 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
    10261 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
    10321 gctcctcgcc cttgctcacc atggtcactg tctgctttgc tgttggtctg ggctcctggg
    10381 tcactggctt actaatggag tctttatgta tgaggactct tatcaattgt tcttctataa
    10441 aggtctgcag tgtttctgtt cgtcccctac atggacaccc agagcctcct aaatacagga
    10501 gccctgataa ctgcacaagt gctcagattc cagcagggtg gaaaatgaga taaagtgtgc
    10561 agatggggag ggggacgtga atgagagatt tgagggatga aaaggatgga tgaacgcatt
    10621 gaaaatagcc cctttcacaa aataatacca gtaaattgtc atataattaa tgaccagacc
    10681 tttacctgta aatgatgtga tgttcatata cagaagaatg ccaacaattt acagatgatt
    10741 ttacaaattc ttaattttcc ttttctaaat ggatttgttc acacattatc tcttcaaagc
    10801 aattgaattt tctggaaaag attggctgtg tgtgtaaaag gtgctactga ggaatttaat
    10861 gtgacatgga atgaagcaaa cagcagccta agacaggatg gcaggaaaaa tgtcacatgt
    10921 ttaaaataag agtgaaacca acgacctgga cttatagaga ctgtgtgtcc ttaggcaaag
    10981 ctcttgtaca aacagtgctt gtgactgtga aattcggggt ttgcctggat tgtgttaaat
    11041 gtgtgcttgg atgtatcata aaaaagaatc ggttctctgt atttgacatt ttctgtaatt
    11101 tatctaaagt tacaactggc tatgcctgat ttttatcact attgaatcaa tcattttaaa
    11161 gaatgttaag tctgctgatt tggagagctc atttacattt attttgaatg tctcttattt
    11221 aacacaactg atttaagctt tagatctaat ctgatcattc aggcgcgcca acacgtgaat
    11281 accacgcgag gccttaggga taacagggta atacgcgtga tctgcgaaga tacggccacg
    11341 ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac
    11401 atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa
    11461 ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca
    11521 tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta
    11581 aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg
    11641 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag
    11701 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta
    11761 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac
    11821 tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac
    11881 tagttctaga gctcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc
    11941 gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat ctgatgaacg
    12001 ggccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
    12061 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    12121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    12181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    12241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    12301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    12361 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    12421 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    12481 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    12541 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    12601 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    12661 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    12721 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    12781 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    12841 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    12901 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    12961 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    13021 tttgcaagca gcagattacg cgcagaaaaa aaggatctca a
//
