LOCUS       pTol2-elavl3:kalta4-attB       11613 bp    DNA     circular SYN 26-JAN-2025
DEFINITION  elavl3-kalta4_D4.
ACCESSION   elavl3-kalta4_D4
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
     promoter        complement(1032..1136)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     rep_origin      complement(1189..1617)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="f1 ori"
     CDS             complement(1622..1777)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
                     /label="lacZα (fragment)"
     misc_feature    1785..1804
                     /ApEinfo_fwdcolor="#9a9aff"
                     /ApEinfo_revcolor="green"
                     /X-Transferred_From="64_pTol2_zp0_5_MCS (digested by
                     NotI)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:qe-hk2ofyl"">64_pTol2_zp0_5_MCS
                     (digested by NotI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:pu-hk2o7ks"">64_pTol2_zp0_5_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:2c-ho07be9"">ptol2-zp05-attb-cmlc2
                     (digested by NdeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:r8-hk2ohg3"">ptol2-zp05-attb-cmlc2</a>)
                     "
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="elavl3-kalta4-attb"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /label="T7 promoter"
     CDS             2906..3547
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="51.4"
                     /match_length="39.6"
                     /fragment="True"
                     /other="CDS"
                     /label="tyr (fragment)"
     strand          3397..3408
                     /Strand_number_=3
                     /Sheet_number_="C"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on protein similarity"
                     /X-Transferred_From="64_pTol2_zp0_5_MCS (digested by
                     NotI)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:qe-hk2ofyl"">64_pTol2_zp0_5_MCS
                     (digested by NotI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Beta Strand (<a
                     href=""urn:local:.:pu-hk2o7ks"">64_pTol2_zp0_5_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Beta Strand (<a
                     href=""urn:local:.:2c-ho07be9"">ptol2-zp05-attb-cmlc2
                     (digested by NdeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Beta Strand (<a
                     href=""urn:local:.:r8-hk2ohg3"">ptol2-zp05-attb-cmlc2</a>)
                     "
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="elavl3-kalta4-attb"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /label="Beta Strand"
     misc_feature    3610..4240
                     /ApEinfo_label="Tol2 site"
                     /ApEinfo_fwdcolor="#ffbdfe"
                     /ApEinfo_revcolor="#ffbdfe"
                     /X-Transferred_From="64_pTol2_zp0_5_MCS (digested by
                     NotI)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site(1) (<a
                     href=""urn:local:.:qe-hk2ofyl"">64_pTol2_zp0_5_MCS
                     (digested by NotI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site(1) (<a
                     href=""urn:local:.:pu-hk2o7ks"">64_pTol2_zp0_5_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site(1) (<a
                     href=""urn:local:.:r8-hk2ohg3"">ptol2-zp05-attb-cmlc2</a>)
                     "
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="elavl3-kalta4-attb"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site(1) (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site(1) (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site(1) (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site(1) (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site(1) (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /modified_by="rreeves"
                     /label="Tol2 site"
     promoter        4246..7113
                     /created_by="rreeves"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="new elavl3 promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 2500bp
                     upstream (<a href=""urn:local:.:1c-hdfzpcl"">new elavl3
                     promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2500bp upstream (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2500bp upstream (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2500bp upstream (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     2500bp upstream (<a href=""urn:local:.:g-hdfx27a"">3</a>)"
                     /modified_by="rreeves"
                     /label="upstream sequence elavl3"
     ORF             7114..7701
                     /created_by="rreeves"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCK007 pME KalTa4"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4? (<a href=""urn:local:.:h-h3apd14"">pCK007 pME
                     KalTa4</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4? (<a href=""urn:local:.:22-h3as4bc"">pCK007 pME
                     KalTa4 - kalta4?</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:133/-435; kalta4 forward (<a
                     href=""urn:local:.:ef-g368ar0"">eGFPbait-E2A-KalTA4-pA</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/-3;
                     kalta4? (<a href=""urn:local:.:1e-h3arc3n"">pCK007 pME
                     KalTa4 translation</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:108/-462; forward primer (<a
                     href=""urn:local:.:1h-h3ard24"">GAL4ff translation</a>)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="elavl3-kalta4-attb"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kalta4?
                     (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4? (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4? (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4? (<a href=""urn:local:.:1p-hdg0hm0"">kalta4</a>)"
                     /modified_by="rreeves"
                     /label="kalta4"
     polyA_signal    7752..7934
                     /ApEinfo_fwdcolor="#ffb78b"
                     /ApEinfo_revcolor="green"
                     /X-Transferred_From="64_pTol2_zp0_5_MCS (digested by
                     NotI)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     PolyA (<a
                     href=""urn:local:.:qe-hk2ofyl"">64_pTol2_zp0_5_MCS
                     (digested by NotI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 PolyA (<a
                     href=""urn:local:.:pu-hk2o7ks"">64_pTol2_zp0_5_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 PolyA (<a
                     href=""urn:local:.:2c-ho07be9"">ptol2-zp05-attb-cmlc2
                     (digested by NdeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 PolyA (<a
                     href=""urn:local:.:r8-hk2ohg3"">ptol2-zp05-attb-cmlc2</a>)
                     "
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:qe-hk2ofyl"">64_pTol2_zp0_5_MCS
                     (digested by NotI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:pu-hk2o7ks"">64_pTol2_zp0_5_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:2c-ho07be9"">ptol2-zp05-attb-cmlc2
                     (digested by NdeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:r8-hk2ohg3"">ptol2-zp05-attb-cmlc2</a>)
                     "
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="elavl3-kalta4-attb"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     PolyA (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     PolyA (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     PolyA (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     PolyA (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     PolyA (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /modified_by="rreeves"
                     /label="SV40 poly(A) signal"
     polyA_signal    complement(7995..8127)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.5"
                     /fragment="False"
                     /other="polyA_signal"
                     /modified_by="rreeves"
                     /label="SV40 poly(A) signal"
     ORF             complement(8178..8894)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="64_pTol2_zp0_5_MCS (digested by
                     NotI)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     reporter (<a
                     href=""urn:local:.:qe-hk2ofyl"">64_pTol2_zp0_5_MCS
                     (digested by NotI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EGFP reporter (<a
                     href=""urn:local:.:pu-hk2o7ks"">64_pTol2_zp0_5_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EGFP reporter (<a
                     href=""urn:local:.:2c-ho07be9"">ptol2-zp05-attb-cmlc2
                     (digested by NdeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EGFP reporter (<a
                     href=""urn:local:.:r8-hk2ohg3"">ptol2-zp05-attb-cmlc2</a>)
                     "
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="elavl3-kalta4-attb"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     reporter (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     reporter (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     reporter (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     reporter (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     reporter (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /modified_by="rreeves"
                     /label="EGFP"
     promoter        complement(8895..9793)
                     /ApEinfo_fwdcolor="#ffffbf"
                     /ApEinfo_revcolor="#ffffbf"
                     /X-Transferred_From="64_pTol2_zp0_5_MCS (digested by
                     NotI)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; cmlc2
                     promoter (<a
                     href=""urn:local:.:qe-hk2ofyl"">64_pTol2_zp0_5_MCS
                     (digested by NotI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cmlc2 promoter (<a
                     href=""urn:local:.:pu-hk2o7ks"">64_pTol2_zp0_5_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cmlc2 promoter (<a
                     href=""urn:local:.:2c-ho07be9"">ptol2-zp05-attb-cmlc2
                     (digested by NdeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cmlc2 promoter (<a
                     href=""urn:local:.:r8-hk2ohg3"">ptol2-zp05-attb-cmlc2</a>)
                     "
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="elavl3-kalta4-attb"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cmlc2
                     promoter (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cmlc2 promoter (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cmlc2 promoter (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /modified_by="rreeves"
                     /label="myl7 (cmlc2) promoter"
     misc_feature    9795..10443
                     /ApEinfo_fwdcolor="#f1beff"
                     /ApEinfo_revcolor="#f1beff"
                     /X-Transferred_From="64_pTol2_zp0_5_MCS (digested by
                     NotI)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:qe-hk2ofyl"">64_pTol2_zp0_5_MCS
                     (digested by NotI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site (<a
                     href=""urn:local:.:pu-hk2o7ks"">64_pTol2_zp0_5_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site (<a
                     href=""urn:local:.:2c-ho07be9"">ptol2-zp05-attb-cmlc2
                     (digested by NdeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site (<a
                     href=""urn:local:.:r8-hk2ohg3"">ptol2-zp05-attb-cmlc2</a>)
                     "
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="elavl3-kalta4-attb"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-4/0;
                     Tol2 site (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-4/0;
                     Tol2 site (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /label="Tol2 site"
     misc_feature    10465..10534
                     /bound_moiety="phage&nbsp;φC31 integrase"
                     /note="attB site for the φC31 integrase (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/10801973""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/10801973"">Grot
                     h et al., 2000</a>)"
                     /X-Transferred_From="pTol2_zp05_MCS_liver_attB_colony_#1
                     (reversed) extraction"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:q3-hk2o9q3"">pTol2_zp05_MCS_liver_attB_
                     colony_#1 (reversed) extraction</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB (<a
                     href=""urn:local:.:2c-ho07be9"">ptol2-zp05-attb-cmlc2
                     (digested by NdeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB (<a
                     href=""urn:local:.:r8-hk2ohg3"">ptol2-zp05-attb-cmlc2</a>)
                     "
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="elavl3-kalta4-attb"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /modified_by="rreeves"
                     /label="attB"
     rep_origin      complement(11025..11613)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
ORIGIN      
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct gaaaacttgg
     1861 atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt atttgtctgc
     1921 atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg actgtgttta
     1981 acagacatta ttacaataat atgtaatagt agtacacact attattatgt aatagtactt
     2041 gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca aaatgactta
     2101 cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta tggacatcgc
     2161 cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc gagtctgagt
     2221 caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga agtccacagc
     2281 ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga
     2341 gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa cttgggctac
     2401 agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat aaagatttta
     2461 atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt ttggatcact
     2521 agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt tggttggttg
     2581 gtagattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     2641 attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga ttcattacag
     2701 aaatgattaa atgcatacat aaaaaactgg gggggggggg ggggatacaa caacacactt
     2761 aagtcacatt tgcctacgta aagaaaagta aagaaaatca atagctatat tttacatctc
     2821 ctttttttgc tgtcttttaa ttagccttgt tttgctgtta tcttgattga aactgtaact
     2881 tcttcacctg cttcttttct ttgtaggttt tgccagccca gaaacaggca tggctgtgca
     2941 aggccagagc accatgcaca atgcgctgca tgtcttcatg aacggctcaa tgtcctcagt
     3001 ccagggctca gccaacgacc ccatcttcct ccttcaccat gctttcattg acaggtaaca
     3061 aacacgtcat gacattagac tgcacagttt ttgacaaagt tcatacaatc tgttgtttat
     3121 agctgctaca attagtgaag tttgtgaatg tacttggatg agcagcgaaa gatcaattga
     3181 gatcaattgt tagagtttgg ttgccctgca gagcaaagaa caaaaaataa tctggtggct
     3241 ttactgcgtg aggttattat tggtggaata gaaacacaaa acataattgc atttatttgt
     3301 ttaatttttt atcttatctt aactttcatc ttgcatattt gtttcttaca tcatttctag
     3361 catctttgag cgctggctaa gaactcatca gcctccccgg tccatctacc cacgtaccaa
     3421 tgcaccaatt ggccacaatg acggctacta catggtgcca ttccttcctc tttataggaa
     3481 tggagactac ctcctgtcca acaatgctct tggatacgag tacgcctacc tgttggaccc
     3541 aggtcattgc acaacaccag aaatgccctc tgatctgcaa aagacgtgaa taatctgttc
     3601 agacacccat atgcactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
     3661 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
     3721 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
     3781 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
     3841 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     3901 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     3961 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     4021 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     4081 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     4141 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccat
     4201 tcgtacgctt aatttcgtat agggataaca gggtaatgat ctaggttaca agctttatcc
     4261 aaattaagcc attttaaaac actgccaatt gaaaatacaa atcctggaaa aaatcgtctt
     4321 tagcgcagtc atttgagcca tcctaatccg ttacctcaga ccataataag aagggataac
     4381 actagctgta gcaatggaac acatctgttt cacacaatca tatctcctgc gccggtgcta
     4441 agcagattca gcgtgatcat aacatgcttt ccactcataa atgtaaattt acaatttgca
     4501 catgtaaaac agacactttt gagatattgg ataaaaaaac aagagtatat tgcttagttt
     4561 catccaccag tcatccccac agcgtttgga aggccataaa aagtgtctaa aatcaatgat
     4621 cattgaaaga gcacaagaga gactcttacg ctgtaatgcc actggggaca aaagtgacag
     4681 tctcttaatg ggctcttctg gaggggctcc tgaacattaa aaattatcag cgaaattacc
     4741 gaaagagctt caagcaactg gcatgcttga tcctctgcgt cggggcggtg aataggtgct
     4801 tcagatgccc tcttacccac gggctggatt cagctgcccc gctaccagcg gagaccccct
     4861 aatgagcctc tgcaattaag tttattcatg ttaagtgtga acggggtgcg tgcggaactg
     4921 tgggcagcta acagacctgg gttctttgtg ccacaagtgc tgcctttatt cggctcacaa
     4981 agcagaaaac aacacccgca cctataatgg cgccctcggc tgggtctaag aaacgtggcg
     5041 agttgacaga gcagagtggg cggggttaag acagactgac agcgggaccc atctccatcc
     5101 tcttattaac gcttaacgag tgccttcctc atgcaatatt catcgccact aatatcatcc
     5161 aagctctgag ctgagctggc cacttatgta aggcaattat gtaaaatatc agacagggcc
     5221 cacactcaga atctgactgg ggtagagacg cgggacgaga accgagagca agaactgaaa
     5281 gtgaaagtga ccactaaagg gaggagagga cagaggggca ggatgtgtca agattaccag
     5341 agaacacttg gccagaaatg cgcaaccatt ggagctctcc ggattaccca aaggttaacg
     5401 agtttgaacg cctctgccca ctcgcccatc tctgatggtt tcccaagaac tcctcaagca
     5461 aaatatatat aattgtgtgt attatgcaca gacacgagaa aatgctgttt ttctgatctg
     5521 cattacagca catttgcccg ccaacgacaa taccacccac tcggtacctc gctgactcct
     5581 gatgcctgat acctgcgcgg tgactgtcta caatctgcat aatcaagaga agttgtgttg
     5641 aagacgagcg ccacacaacc gtttccacaa ggtcacccaa ggccggtgca gatgtaggtg
     5701 aggtctccat aaacagactg aaataaacac atcctccgct gggaacaaca accccctcac
     5761 gcctcatgca tttccataag cctacatgca tctcttccaa cttatggaga ctcgcaccta
     5821 ccaacatccg cacaacaaag atatacagag cgcgctccct caggtcaagg ccctgtgggg
     5881 gtctgtgcag aaataggtca tttgtcacac atcaagtcct ggggcaggag atgcattata
     5941 gatgagacca aacagcctgt ctcggtgagc tctacccact ccctgagact agaaatgggg
     6001 gaagggagct tgagataaca accgctgcaa tcactgtgtc gatgtttaat atcagcacca
     6061 accgggaaca ataaggagat gggtgcattc atgttcacat cttaccagtc aagtatcatc
     6121 gaaccggctt gataaccaca cctcgtgtaa tagctgagca gatagttgtc attttaaagc
     6181 gttggccttt gtcgattatg taatgcgcac attcaacaca tggtaatata gaaacggtta
     6241 tgtcgaggtt gttttgtcca gagatgacct tcacacagtt acagccgctc tgcatccaca
     6301 caaatggagg acttaatcgt ggactgcatt cttagaaatg atctacaaag acaaataatg
     6361 tgaaatcaag aaaggacaaa atttaagtaa ggggatgagg gagagagaga acgaggggca
     6421 aggagaaagc atggctcctg tctttttctg cacccatctg ttcggagtgc aggtggagct
     6481 ctattcactc agctctgcat gtgtgtttgg ggggggcagg aagaaaggga gggcaaaagg
     6541 aagagtggag agatggtggg ggctggaggg atggggggtt ctcggtgatc tctcctgaag
     6601 gggataatgg gagagcagcg ctttgcaatg gctgccatgt agtaccctcc ctgcacaatt
     6661 agccaatcag cagcaagctc tgccagccag aaggacacat aaaagaagaa cattgcagca
     6721 gaggcacaga aggagcctgc gaggagctgg gaaatacaca cacaacagca gaaccacaac
     6781 accctcccct ggacacaccc tactggggat cactgctttt ctttttttct gaaccatcgc
     6841 ccacgccaca cggagagaaa tctctctctc atcatcatcc tgaagaaaac ccccttatcc
     6901 tcattttcac actgctgagg aaaacctaca atcgcacggg ctgagatttc ctggcgaaga
     6961 ctgtcctttt tcctttttct ttttttttct tttcctttgg aaactgacat ttgcatttct
     7021 ccattccaag ccacggcgta ataatatctg caatccagcc tgaagacctg caaatcgaag
     7081 gacctagatc actatcatct ttgtacgtca agaatgaaac tgctctcatc catcgagcaa
     7141 gcctgcgaca tttgtcggct taagaagctg aaatgctcca aggaaaagcc gaaatgtgcc
     7201 aaatgcctga agaacaattg ggaatgtcgt tactctccca aaaccaagcg aagtccactc
     7261 acaagggctc atctgaccga agtggagagc aggctagaga gactggaaca actctttttg
     7321 ctcatcttcc ctagagagga ccttgacatg atcctcaaga tggattctct ccaggatatt
     7381 aaagcccttt tgactggctt attcgtccag gacaatgtga acaaagacgc tgtgacagac
     7441 cgattggcaa gtgtcgagac cgatatgcct ctgacactga gacagcacag aatcagcgct
     7501 acttcctcaa gcgaagagtc ttctaacaag ggacagagac agctgactgt ttcgagcagg
     7561 tcgaccccgt ccccggccga cgccctggac gacggcgacc tggacatgct gcctgctgat
     7621 gctctcgatg atttcgatct ggatatgctc ccggccgacg ccctggacga ctacgacctg
     7681 gacatcctcc cgggtaacta acaaactcga gaaagctagc aaaccatggt acgatgatga
     7741 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7801 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7861 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     7921 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     7981 tctagatcag atctcttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8041 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8101 atcaatgtat cttatcatgt ctggatctac gtaatacgac tcactatagt tctagaggct
     8161 cgagaggggc cgctttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     8221 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
     8281 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     8341 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
     8401 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
     8461 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     8521 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     8581 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     8641 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     8701 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     8761 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     8821 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     8881 cccttgctca ccatggtcac tgtctgcttt gctgttggtc tgggctcctg ggtcactggc
     8941 ttactaatgg agtctttatg tatgaggact cttatcaatt gttcttctat aaaggtctgc
     9001 agtgtttctg ttcgtcccct acatggacac ccagagcctc ctaaatacag gagccctgat
     9061 aactgcacaa gtgctcagat tccagcaggg tggaaaatga gataaagtgt gcagatgggg
     9121 agggggacgt gaatgagaga tttgagggat gaaaaggatg gatgaacgca ttgaaaatag
     9181 cccctttcac aaaataatac cagtaaattg tcatataatt aatgaccaga cctttacctg
     9241 taaatgatgt gatgttcata tacagaagaa tgccaacaat ttacagatga ttttacaaat
     9301 tcttaatttt ccttttctaa atggatttgt tcacacatta tctcttcaaa gcaattgaat
     9361 tttctggaaa agattggctg tgtgtgtaaa aggtgctact gaggaattta atgtgacatg
     9421 gaatgaagca aacagcagcc taagacagga tggcaggaaa aatgtcacat gtttaaaata
     9481 agagtgaaac caacgacctg gacttataga gactgtgtgt ccttaggcaa agctcttgta
     9541 caaacagtgc ttgtgactgt gaaattcggg gtttgcctgg attgtgttaa atgtgtgctt
     9601 ggatgtatca taaaaaagaa tcggttctct gtatttgaca ttttctgtaa tttatctaaa
     9661 gttacaactg gctatgcctg atttttatca ctattgaatc aatcatttta aagaatgtta
     9721 agtctgctga tttggagagc tcatttacat ttattttgaa tgtctcttat ttaacacaac
     9781 tgatttaagc tttagatcta atctgatcat tcaggcgcgc caacacgtga ataccacgcg
     9841 aggccttagg gataacaggg taatacgcgt gatctgcgaa gatacggcca cgggtgctct
     9901 tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc
     9961 tccattttct tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt
    10021 gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac
    10081 caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac
    10141 tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc
    10201 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
    10261 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
    10321 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
    10381 tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta
    10441 gagctcgacg atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt
    10501 gggctccccg ggcgcgtact ccacctcacc catctggtcc atctgatgaa cgggccaccg
    10561 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
    10621 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    10681 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    10741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    10861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    11221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    11281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    11401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11461 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    11581 cagcagatta cgcgcagaaa aaaaggatct caa
//