LOCUS       pTol2-rtn1a:kalta4-attB       10430 bp    DNA     circular SYN 26-JAN-2025
DEFINITION  Rtn1a-kalta4__colony_G4.
ACCESSION   Rtn1a-kalta4__colony_G4
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="AmpR"
     promoter        complement(1032..1136)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="AmpR promoter"
     rep_origin      complement(1189..1617)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 ori
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="f1 ori"
     CDS             complement(1622..1777)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacZα
                     (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="lacZα (fragment)"
     misc_feature    1785..1804
                     /ApEinfo_fwdcolor="#9a9aff"
                     /ApEinfo_revcolor="green"
                     /X-Transferred_From="elavl3-kalta4-attb"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="T7 promoter"
     CDS             2907..3548
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="51.4"
                     /match_length="39.6"
                     /fragment="True"
                     /other="CDS"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tyr
                     (fragment) (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="tyr (fragment)"
     strand          3398..3409
                     /Strand_number_=3
                     /Sheet_number_="C"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on protein similarity"
                     /X-Transferred_From="elavl3-kalta4-attb"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Beta Strand (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Beta Strand (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Beta Strand (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Beta Strand (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Beta
                     Strand (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Beta Strand"
     misc_feature    3611..4241
                     /ApEinfo_label="Tol2 site"
                     /ApEinfo_fwdcolor="#ffbdfe"
                     /ApEinfo_revcolor="#ffbdfe"
                     /X-Transferred_From="elavl3-kalta4-attb"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site(1) (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site(1) (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site(1) (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site(1) (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site(1) (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /modified_by="rreeves"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Tol2 site"
     promoter        4242..5930
                     /created_by="rreeves"
                     /label="rtn1a upstream sequence"
     ORF             5931..6518
                     /created_by="rreeves"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="elavl3-kalta4-attb"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4? (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4? (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4? (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     kalta4? (<a href=""urn:local:.:1p-hdg0hm0"">kalta4</a>)"
                     /modified_by="rreeves"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kalta4
                     (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="kalta4"
     polyA_signal    6569..6751
                     /ApEinfo_fwdcolor="#ffb78b"
                     /ApEinfo_revcolor="green"
                     /X-Transferred_From="elavl3-kalta4-attb"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     PolyA (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 PolyA (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 PolyA (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 PolyA (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 PolyA (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-81/92;
                     primer AP101_R SV40 (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /modified_by="rreeves"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/63;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="SV40 poly(A) signal"
     polyA_signal    complement(6812..6944)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.5"
                     /fragment="False"
                     /other="polyA_signal"
                     /modified_by="rreeves"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="SV40 poly(A) signal"
     ORF             complement(6995..7711)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /note="Derived using Geneious Prime 2022.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="elavl3-kalta4-attb"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP
                     reporter (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EGFP reporter (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EGFP reporter (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EGFP reporter (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EGFP reporter (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /modified_by="rreeves"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; EGFP (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="EGFP"
     promoter        complement(7712..8610)
                     /ApEinfo_fwdcolor="#ffffbf"
                     /ApEinfo_revcolor="#ffffbf"
                     /X-Transferred_From="elavl3-kalta4-attb"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; cmlc2
                     promoter (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cmlc2 promoter (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cmlc2 promoter (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /modified_by="rreeves"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; myl7
                     (cmlc2) promoter (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="myl7 (cmlc2) promoter"
     misc_feature    8612..9260
                     /ApEinfo_fwdcolor="#f1beff"
                     /ApEinfo_revcolor="#f1beff"
                     /X-Transferred_From="elavl3-kalta4-attb"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Tol2 site (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-4/0;
                     Tol2 site (<a
                     href=""urn:local:.:wd-hdgsblr"">pTol2_shortelavl4-kalta4_M
                     CS_liver</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-4/0;
                     Tol2 site (<a
                     href=""urn:local:.:v3-hdgs4nv"">pTol2_zp05_MCS_liver
                     (digested by BamHI, SalI) Fragment 2</a>)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tol2
                     site (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="Tol2 site"
     misc_feature    9282..9351
                     /bound_moiety="phage&nbsp;φC31 integrase"
                     /note="attB site for the φC31 integrase (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/10801973""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/10801973"">Grot
                     h et al., 2000</a>)"
                     /X-Transferred_From="elavl3-kalta4-attb"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB
                     (<a
                     href=""urn:local:.:45-htvkq4o"">elavl3-kalta4-attb</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB (<a
                     href=""urn:local:.:1c-htvkoth"">zp0_5-attB,_colony_A
                     (reversed) (digested by SalI, BamHI) Fragment 2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB (<a
                     href=""urn:local:.:2u-hu0tc2f"">elavl3-kalta4-attb
                     (digested by NaeI)</a>)"
                     /modified_by="rreeves"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="attB"
     rep_origin      complement(9842..10430)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTol2-elavl3:kalta4-attB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori (<a
                     href=""urn:local:.:w-iqmbrur"">pTol2-elavl3:kalta4-attB</a
                     >)"
                     /label="ori"
ORIGIN      
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct gaaaacttgg
     1861 atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt atttgtctgc
     1921 atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg actgtgttta
     1981 acagacatta ttacaataat atgtaatagt agtacacact attattatgt aatagtactt
     2041 gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca aaatgactta
     2101 cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta tggacatcgc
     2161 cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc gagtctgagt
     2221 caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga agtccacagc
     2281 ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga
     2341 gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa cttgggctac
     2401 agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat aaagatttta
     2461 atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt ttggatcact
     2521 agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt tggttggttg
     2581 gtagattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     2641 attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga ttcattacag
     2701 aaatgattaa atgcatacat aaaaaactgg gggggggggg gggggataca acaacacact
     2761 taagtcacat ttgcctacgt aaagaaaagt aaagaaaatc aatagctata ttttacatct
     2821 cctttttttg ctgtctttta attagccttg ttttgctgtt atcttgattg aaactgtaac
     2881 ttcttcacct gcttcttttc tttgtaggtt ttgccagccc agaaacaggc atggctgtgc
     2941 aaggccagag caccatgcac aatgcgctgc atgtcttcat gaacggctca atgtcctcag
     3001 tccagggctc agccaacgac cccatcttcc tccttcacca tgctttcatt gacaggtaac
     3061 aaacacgtca tgacattaga ctgcacagtt tttgacaaag ttcatacaat ctgttgttta
     3121 tagctgctac aattagtgaa gtttgtgaat gtacttggat gagcagcgaa agatcaattg
     3181 agatcaattg ttagagtttg gttgccctgc agagcaaaga acaaaaaata atctggtggc
     3241 tttactgcgt gaggttatta ttggtggaat agaaacacaa aacataattg catttatttg
     3301 tttaattttt tatcttatct taactttcat cttgcatatt tgtttcttac atcatttcta
     3361 gcatctttga gcgctggcta agaactcatc agcctccccg gtccatctac ccacgtacca
     3421 atgcaccaat tggccacaat gacggctact acatggtgcc attccttcct ctttatagga
     3481 atggagacta cctcctgtcc aacaatgctc ttggatacga gtacgcctac ctgttggacc
     3541 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga ataatctgtt
     3601 cagacaccca tatgcactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
     3661 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
     3721 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
     3781 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
     3841 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
     3901 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
     3961 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
     4021 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
     4081 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
     4141 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggcca
     4201 ttcgtacgct taatttcgta tagggataac agggtaatga tctagggtac tctaaaccag
     4261 cgatgcccaa aaactagggc ccgctggcca aagttggccc agaataacct ttgatttggc
     4321 caccccatct gagaagagaa cgagaatgat agggaaggtt gaagcaaacc agagattgtc
     4381 aaaacaattt tactttttgt ttgtttgttt aattgttaag ctacaaaaaa gcaaactgaa
     4441 attgaatgtt tcagataaat gttctgaatg aatcagattt ttaatgttaa actgtaaata
     4501 ctgttacttg atggaagaca gtgttctaaa tgggatttat atatttttat tgtaaaaaaa
     4561 ttatttaatt ttaagtatga ataaattagg aatttaccat ttgaaacttt attgtaatac
     4621 actttagtat attaaccttt agcccgccaa cctaaatcaa atttggtttt tggctcttca
     4681 taagaaaaaa tgtgggcatc cctgctctaa accttctcac tcagtatgca gaacatgtga
     4741 taagtcacat acaatttgtt gagcatgtct taatatggcc tgttcataaa tttgctgcag
     4801 tgacatctgc tctgatacac caataataaa taaacataca tttcaatggg gctggtttca
     4861 tggtgatgac atgaaagagt cctattttgt atacttaaag aatccttcag taaaaattct
     4921 tttccaataa tagatttttt ttcacagtgc gaagacttca taataggaca attttcagca
     4981 gtaaagaacc ttttgtggga taaaataatt cctagttgtt cttgatatat caattataat
     5041 gtcttatgga accactgagg tcagaaagaa ttttttattt tataatattg ttctccaaat
     5101 atttcactga cccacaacaa gcacttctcc agcctctctt ggatcccagc ttaaaaggtg
     5161 ccaaattgat ttattgtcaa ttatgtttca atatgacctt tagagaatgt gtgaaggaat
     5221 tgtgaagttt ccaaaaagtt ttattaaatt gagttctctg gggcctttag aaaattctga
     5281 ttagtttaac agttattaat ttatgggatt agttcatgtt acattacact aattattcgt
     5341 tttaaacaaa tcattttgtt tcaattaaat aatacccttt ttaatttcgt tgttataatg
     5401 ctgattactg tttttatgat gtttatgata tttataacca agtaaaatct tgcattttca
     5461 caaaagttcg tctgcaatgt tgttaaagtc agtgtttgtg aatagaaaca aaataaatca
     5521 gactccaccc tttttgctcc gacaacaatg ccactgaggt cacatgacca tgctcatgtt
     5581 tatgtatgag ctttgtttgg tagggtgtgg cctctttgat ctgactccat catcatcacc
     5641 accacccccg tcgggctctg catatggtac ggtccgctct ctggctctgc ggtggaggct
     5701 gcagtgttta ccgtttcatc caacagagcg gctcctctcc tctcctttct ctttccgtct
     5761 tatctgccct tttcctcgtt aagcagcagc atcctctctc tctctctgct ctctcatcta
     5821 atttctggcc attcatgcgg ttcggacgtg tgagatagtg ggtctctgaa agacatacga
     5881 ccagcgaaag ggaaaggaaa aagacgacag cagtgagaga agcgacagac atgaaactgc
     5941 tctcatccat cgagcaagcc tgcgacattt gtcggcttaa gaagctgaaa tgctccaagg
     6001 aaaagccgaa atgtgccaaa tgcctgaaga acaattggga atgtcgttac tctcccaaaa
     6061 ccaagcgaag tccactcaca agggctcatc tgaccgaagt ggagagcagg ctagagagac
     6121 tggaacaact ctttttgctc atcttcccta gagaggacct tgacatgatc ctcaagatgg
     6181 attctctcca ggatattaaa gcccttttga ctggcttatt cgtccaggac aatgtgaaca
     6241 aagacgctgt gacagaccga ttggcaagtg tcgagaccga tatgcctctg acactgagac
     6301 agcacagaat cagcgctact tcctcaagcg aagagtcttc taacaaggga cagagacagc
     6361 tgactgtttc gagcaggtcg accccgtccc cggccgacgc cctggacgac ggcgacctgg
     6421 acatgctgcc tgctgatgct ctcgatgatt tcgatctgga tatgctcccg gccgacgccc
     6481 tggacgacta cgacctggac atcctcccgg gtaactaaca aactcgagaa agctagcaaa
     6541 ccatggtacg atgatgatcc agacatgata agatacattg atgagtttgg acaaaccaca
     6601 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     6661 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     6721 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     6781 atggctgatt atgatcctct agatcagatc tcttgtttat tgcagcttat aatggttaca
     6841 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6901 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctacgta atacgactca
     6961 ctatagttct agaggctcga gaggggccgc tttacttgta cagctcgtcc atgccgagag
     7021 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     7081 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     7141 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     7201 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     7261 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     7321 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     7381 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     7441 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     7501 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     7561 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     7621 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     7681 cggtgaacag ctcctcgccc ttgctcacca tggtcactgt ctgctttgct gttggtctgg
     7741 gctcctgggt cactggctta ctaatggagt ctttatgtat gaggactctt atcaattgtt
     7801 cttctataaa ggtctgcagt gtttctgttc gtcccctaca tggacaccca gagcctccta
     7861 aatacaggag ccctgataac tgcacaagtg ctcagattcc agcagggtgg aaaatgagat
     7921 aaagtgtgca gatggggagg gggacgtgaa tgagagattt gagggatgaa aaggatggat
     7981 gaacgcattg aaaatagccc ctttcacaaa ataataccag taaattgtca tataattaat
     8041 gaccagacct ttacctgtaa atgatgtgat gttcatatac agaagaatgc caacaattta
     8101 cagatgattt tacaaattct taattttcct tttctaaatg gatttgttca cacattatct
     8161 cttcaaagca attgaatttt ctggaaaaga ttggctgtgt gtgtaaaagg tgctactgag
     8221 gaatttaatg tgacatggaa tgaagcaaac agcagcctaa gacaggatgg caggaaaaat
     8281 gtcacatgtt taaaataaga gtgaaaccaa cgacctggac ttatagagac tgtgtgtcct
     8341 taggcaaagc tcttgtacaa acagtgcttg tgactgtgaa attcggggtt tgcctggatt
     8401 gtgttaaatg tgtgcttgga tgtatcataa aaaagaatcg gttctctgta tttgacattt
     8461 tctgtaattt atctaaagtt acaactggct atgcctgatt tttatcacta ttgaatcaat
     8521 cattttaaag aatgttaagt ctgctgattt ggagagctca tttacattta ttttgaatgt
     8581 ctcttattta acacaactga tttaagcttt agatctaatc tgatcattca ggcgcgccaa
     8641 cacgtgaata ccacgcgagg ccttagggat aacagggtaa tacgcgtgat ctgcgaagat
     8701 acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg
     8761 atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt
     8821 ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac
     8881 caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa
     8941 taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa
     9001 aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg
     9061 agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt
     9121 ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag
     9181 taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag
     9241 cagatccact agttctagag ctcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     9301 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     9361 tgatgaacgg gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg
     9421 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9481 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9541 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9601 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9661 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9721 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9781 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9841 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9901 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9961 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10021 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10081 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10141 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10201 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10261 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10321 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10381 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
//