LOCUS       Exported                2459 bp ds-DNA     linear   UNA 27-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 16, 2025 from SnapGene 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     protein_bind    357..381
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     misc_feature    complement(406..415)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(420..429)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(435..444)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(449..458)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(464..473)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(478..487)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
                     /note="color: #00ff00; direction: LEFT"
     protein_bind    complement(519..543)
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    550..617
                     /locus_tag="cFos minimal promoter"
                     /label=cFos minimal promoter
                     /note="color: #00ffff"
     CDS             complement(709..720)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /note="color: #cc99b2
                     Cleavage site after base 708"
                     /translation="WELQ"
     regulatory      781..790
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     misc_feature    787..1632
                     /locus_tag="D2eGFP"
                     /label=D2eGFP
                     /note="color: #80ff80"
     primer_bind     1472..1490
                     /label=eGFP-Pest F
                     /note="color: black; sequence: GGATCACTCTCGGCATGGA; added: 
                     2024-03-27"
     misc_feature    1504..1632
                     /locus_tag="PEST domain"
                     /label=PEST domain
                     /note="color: #ff8080"
     polyA_signal    complement(1642..1776)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(1799..1882)
                     /codon_start=1
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.9"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="DHNQPYHICRGFTCFKKPPTPPPEPET"
     primer_bind     complement(1931..1950)
                     /label=eGFP-Pest R
                     /note="color: black; sequence: GCACAGTCCAAAATCAGCCA; added:
                     2024-03-27"
     misc_feature    2215..2414
                     /locus_tag="Tol2 site 3'"
                     /label=Tol2 site 3'
                     /note="color: #ff80c0"
     misc_feature    2266..2385
                     /locus_tag="Tol2"
                     /label=Tol2
                     /note="color: #00ff80"
     misc_feature    2386..2414
                     /locus_tag="tol2"
                     /label=tol2
                     /note="color: #80ff00"
ORIGIN
        1 aagcttttaa tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga
       61 actgtttgta tttaattatc aaactataag acagacaatc taatgccagt acacgctact
      121 caaagttgta aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt
      181 caagcctagc tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg
      241 cagccgatgc gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg
      301 tgagcagaga ctccctggtg tctgaaacac aggccagatg ggccctcgag atatcaacaa
      361 gtttgtacaa aaaagcaggc ttcctgtgaa tgcatgcacg gtcgagggac tttccgctgg
      421 ggactttccg tcgagggact ttccgctggg gactttccgt cgagggactt tccgctgggg
      481 actttccgtc gacctgcaga attcgaagct tgagctcgac ccagctttct tgtacaaagt
      541 ggttgatatc cagtgacgta ggaagtccat ccattcacag cgcttctata aaggcgccag
      601 ctgaggcgcc tactactcca accgcgactg cagcgagcaa ctgagaagac tggatagagc
      661 cggcggttcc gcgaacgagc agtgaccgcg ctcccaccca gctctgctct gcagctccca
      721 ccagtgtcta cccctggacc ccttgccggg ctttccccaa acttcgagga tccaccggtc
      781 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      841 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      901 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      961 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1021 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1081 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1141 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1201 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1261 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1321 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1381 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1441 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1501 aagaagctta gccatggctt cccgccggag gtggaggagc aggatgatgg cacgctgccc
     1561 atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc ttctgctagg
     1621 atcaatgtgt agatcgatga tgatccagac atgataagat acattgatga gtttggacaa
     1681 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     1741 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     1801 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     1861 tgtggtatgg ctgattatga tcctctagat cagatctgcg aagatacggc cacgggtgct
     1921 cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa tcacatactt
     1981 cctccatttt cttccactga ttgactgtta taatttccct aatttccagg tcaaggtgct
     2041 gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg aacatgtctg
     2101 accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca tttaaattaa
     2161 actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag aatgaagtga
     2221 tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt
     2281 tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag
     2341 taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt
     2401 actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat ccactagtt
//
