LOCUS       Exported                7321 bp ds-DNA     circular SYN 02-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   ISU Biology IT
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 20, 2025 from SnapGene 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    585..609
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    complement(634..643)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(648..657)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(663..672)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(677..686)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(692..701)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(706..715)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     protein_bind    complement(747..771)
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    778..845
                     /locus_tag="cfos (incomplete)"
                     /label=cfos (incomplete)
     CDS             complement(937..948)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     976..1016
                     
     CDS             996..1670
                     /codon_start=1
                     /label=mRFP
                     /note="/ApEinfo_fwdcolor=#ff0000"
                     /note="/ApEinfo_label=mRFP"
                     /note="/ApEinfo_revcolor=#ff0000"
                     /note="/vntifkey=4"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1653..1697)
                     
     misc_feature    1678..1799
                     /locus_tag="PEST domain"
                     /label=PEST domain
     primer_bind     1678..1716
                     /label=Pest_domain_F
     primer_bind     complement(1776..1823)
                     /label=Pest_domain_R
     misc_feature    1804..1805
                     /label=ClaI
     polyA_signal    complement(1809..1943)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1966..2049)
                     /codon_start=1
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.9"
                     /note="/other=CDS"
                     /translation="DHNQPYHICRGFTCFKKPPTPPPEPET"
     misc_feature    2382..2581
                     /locus_tag="tol2(1)"
                     /label=tol2
     misc_feature    2433..2552
                     /locus_tag="Tol2"
                     /label=Tol2
     misc_feature    2553..2581
                     /locus_tag="tol2"
                     /label=tol2
     promoter        complement(2668..2686)
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(2731..2747)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(2755..2785)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(2800..2821)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(2834..2926)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(3109..3697)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(3868..4728)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4729..4833)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      complement(4886..5314)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(5319..5475)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.4"
                     /note="/match_length=90.2"
                     /note="/other=CDS"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFRQLA**RRGPHRSPFPT
                     VAQPEWR"
     promoter        5483..5501
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
ORIGIN
        1 acaggtcaga ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt
       61 actcaagtga aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct
      121 ggctagaatc ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat
      181 taaggaagta aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc
      241 tcaaaaaaca ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt
      301 taattatcaa actataagac agacaatcta atgccagtac acgctactca aagttgtaaa
      361 acctcagatt taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc
      421 ttttggggct gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg
      481 gaagaggtgt attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact
      541 ccctggtgtc tgaaacacag gccagatggg ccctcgagat atcaacaagt ttgtacaaaa
      601 aagcaggctt cctgtgaatg catgcacggt cgagggactt tccgctgggg actttccgtc
      661 gagggacttt ccgctgggga ctttccgtcg agggactttc cgctggggac tttccgtcga
      721 cctgcagaat tcgaagcttg agctcgaccc agctttcttg tacaaagtgg ttgatatcca
      781 gtgacgtagg aagtccatcc attcacagcg cttctataaa ggcgccagct gaggcgccta
      841 ctactccaac cgcgactgca gcgagcaact gagaagactg gatagagccg gcggttccgc
      901 gaacgagcag tgaccgcgct cccacccagc tctgctctgc agctcccacc agtgtctacc
      961 ccggacccct tgccgggctt tccccaaact tcgagatggc ctcctccgag gacgtcatca
     1021 aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga
     1081 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     1141 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc cagtacggct
     1201 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     1261 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     1321 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     1381 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     1441 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gatgaggctg aagctgaagg
     1501 acggcggcca ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc
     1561 tgcccggcgc ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca
     1621 ccatcgtgga acagtacgag cgcgccgagg gccgccactc caccggcgcc aagcttagcc
     1681 atggcttccc gccggaggtg gaggagcagg atgatggcac gctgcccatg tcttgtgccc
     1741 aggagagcgg gatggaccgt caccctgcag cctgtgcttc tgctaggatc aatgtgtaga
     1801 tcgatgatga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1861 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1921 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1981 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     2041 attatgatcc tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg
     2101 ctgattttgg actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt
     2161 ccactgattg actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta
     2221 atagatgtga catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat
     2281 aatgtgaaaa cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc
     2341 gcaattcaat tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata
     2401 agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa
     2461 atgtaattaa gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc
     2521 cccaaaaata atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct
     2581 ggttcttgac cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc
     2641 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     2701 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2761 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2821 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2881 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2941 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3001 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3061 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3121 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3181 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3241 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3301 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3361 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3421 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3481 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3541 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3601 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3661 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3721 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3781 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3841 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3901 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3961 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4021 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4081 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4141 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4201 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4261 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4321 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4381 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4441 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4501 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4561 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4621 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4681 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4741 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4801 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     4861 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     4921 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     4981 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     5041 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     5101 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     5161 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     5221 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     5281 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     5341 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaagggggg
     5401 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     5461 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccaaata
     5521 gtaggaatta cccacctgta caagtgctga aaacttggat gaataagccc gtttgccatt
     5581 ttctactgct attttaaatc ttttctgtat ttgtctgcat ttgtctttac ccttgaataa
     5641 atgttttagt tgtttttttt caattatgac tgtgtttaac agacattatt acaataatat
     5701 gtaatagtag tacacactat tattatgtaa tagtacttgt tgactgtatt tgagaactgg
     5761 accgagtgag tgttacgtca cccattcaaa atgacttact tctggctcca acaaaatgaa
     5821 gttaattcag ttgccatttt tcactgtatg gacatcgccg tgttggagct agacgttgcc
     5881 aataagcaag aaagagccga gatgcgtcga gtctgagtca ccgttcctat ggcaacccct
     5941 ctaaccaatc agaagtaagc ttgttggaag tccacagcct accacttgaa agcgggctgc
     6001 acaaaatctg tcaaacgttt tgaacgttgg atgtgagagc acatactttt attaaggcat
     6061 ctggttggtc agtttataat atcaacaact tgggctacag aaaagaaaag ttattacaga
     6121 aattatgatt aacaagtaca tgttaaataa agattttaat atgaatgcca ccactggagc
     6181 attcatgcca tttggagctt cttcctgttt ggatcactag aaggaggagg tcactcatta
     6241 cagttctcat atacagtcgt tggttggttg gttggttggt agattgattg attgattgat
     6301 tgattgattg attgattgat tgattgattg attgattgat tgattgattg attgattggt
     6361 agtcaaaata agaaataatt tccacagatt cattacagaa atgattaaat gcatacataa
     6421 aaaactgggg gggggggggg atacaacaac acacttaagt cacatttgcc tacgtaaaga
     6481 aaagtaaaga aaatcaatag ctatatttta catctccttt ttttgctgtc ttttaattag
     6541 ccttgttttg ctgttatctt gattgaaact gtaacttctt cacctgcttc ttttctttgt
     6601 aggttttgcc agcccagaaa caggcatggc tgtgcaaggc cagagcacca tgcacaatgc
     6661 gctgcatgtc ttcatgaacg gctcaatgtc ctcagtccag ggctcagcca acgaccccat
     6721 cttcctcctt caccatgctt tcattgacag gtaacaaaca cgtcatgaca ttagactgca
     6781 cagtttttga caaagttcat acaatctgtt gtttatagct gctacaatta gtgaagtttg
     6841 tgaatgtact tggatgagca gcgaaagatc aattgagatc aattgttaga gtttggttgc
     6901 cctgcagagc aaagaacaaa aaataatctg gtggctttac tgcgtgaggt tattattggt
     6961 ggaatagaaa cacaaaacat aattgcattt atttgtttaa ttttttatct tatcttaact
     7021 ttcatcttgc atatttgttt cttacatcat ttctagcatc tttgagcgct ggctaagaac
     7081 tcatcagcct ccccggtcca tctacccacg taccaatgca ccaattggcc acaatgacgg
     7141 ctactacatg gtgccattcc ttcctcttta taggaatgga gactacctcc tgtccaacaa
     7201 tgctcttgga tacgagtacg cctacctgtt ggacccaggt cattgcacaa caccagaaat
     7261 gccctctgat ctgcaaaaga cgtgaatatc tgttcagaca cccatatcca ctctgttcca
     7321 c
//
