LOCUS       Exported                7212 bp ds-DNA     circular SYN 11-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   ISU Biology IT
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 20, 2025 from SnapGene 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    585..609
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    complement(634..643)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(648..657)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(663..672)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(677..686)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(692..701)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     misc_feature    complement(706..715)
                     /locus_tag="NFkB(5)"
                     /label=NFkB
     protein_bind    complement(747..771)
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    778..845
                     /locus_tag="cfos (incomplete)"
                     /label=cfos (incomplete)
     CDS             complement(937..948)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     993..1036
                     
     regulatory      1008..1012
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1013..1690
                     /codon_start=1
                     /product="Kaede green-to-red photoconvertible fluorescent 
                     protein (Ando et al., 2002)"
                     /label=Kaede
                     /translation="MSLIKPEMKIKLLMEGNVNGHQFVIEGDGKGHPFEGKQSMDLVVK
                     EGAPLPFAYDILTTAFHYGNRVFAKYPDHIPDYFKQSFPKGFSWERSLMFEDGGVCIAT
                     NDITLKGDTFFNKVRFDGVNFPPNGPVMQKKTLKWEASTEKMYLRDGVLTGDITMALLL
                     KGDVHYRCDFRTTYKSRQEGVKLPGYHFVDHCISILRHDKDYNEVKLYEHAVAHSGLPD
                     NVK"
     primer_bind     complement(1673..1714)
                     
     protein_bind    complement(1691)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    1695..1696
                     /label=ClaI
     polyA_signal    complement(1700..1834)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     primer_bind     complement(1739..1759)
                     /label=pCS2-R
     CDS             complement(1857..1940)
                     /codon_start=1
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.9"
                     /note="/other=CDS"
                     /translation="DHNQPYHICRGFTCFKKPPTPPPEPET"
     primer_bind     1909..1931
                     /label=cpE55_sequence_F
     misc_feature    2273..2472
                     /locus_tag="tol2(1)"
                     /label=tol2
     misc_feature    2324..2443
                     /locus_tag="Tol2"
                     /label=Tol2
     misc_feature    2444..2472
                     /locus_tag="tol2"
                     /label=tol2
     promoter        complement(2559..2577)
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     complement(2560..2576)
                     /label=ISU-T3
     protein_bind    complement(2622..2638)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(2646..2676)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(2691..2712)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(2725..2817)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(3000..3588)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(3759..4619)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4620..4724)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      complement(4777..5205)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(5210..5366)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.4"
                     /note="/match_length=90.2"
                     /note="/other=CDS"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFRQLA**RRGPHRSPFPT
                     VAQPEWR"
     promoter        5374..5392
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     5374..5390
                     /label=T7 primer
     primer_bind     5375..5391
                     /label=ISU-T7-1
ORIGIN
        1 acaggtcaga ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt
       61 actcaagtga aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct
      121 ggctagaatc ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat
      181 taaggaagta aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc
      241 tcaaaaaaca ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt
      301 taattatcaa actataagac agacaatcta atgccagtac acgctactca aagttgtaaa
      361 acctcagatt taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc
      421 ttttggggct gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg
      481 gaagaggtgt attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact
      541 ccctggtgtc tgaaacacag gccagatggg ccctcgagat atcaacaagt ttgtacaaaa
      601 aagcaggctt cctgtgaatg catgcacggt cgagggactt tccgctgggg actttccgtc
      661 gagggacttt ccgctgggga ctttccgtcg agggactttc cgctggggac tttccgtcga
      721 cctgcagaat tcgaagcttg agctcgaccc agctttcttg tacaaagtgg ttgatatcca
      781 gtgacgtagg aagtccatcc attcacagcg cttctataaa ggcgccagct gaggcgccta
      841 ctactccaac cgcgactgca gcgagcaact gagaagactg gatagagccg gcggttccgc
      901 gaacgagcag tgaccgcgct cccacccagc tctgctctgc agctcccacc agtgtctacc
      961 ccggacccct tgccgggctt tccccaaact tcgaggatcc accggtcgcc acatgagtct
     1021 gattaaacca gaaatgaaga tcaagctgct tatggaaggc aatgtaaacg ggcaccagtt
     1081 tgttattgag ggagatggaa aaggccatcc ttttgaggga aaacagagta tggaccttgt
     1141 agtcaaagaa ggcgcacctc tcccttttgc ctacgatatc ttgacaacag cattccatta
     1201 tggtaacagg gtttttgcta aatacccaga ccatatacca gactacttca agcagtcgtt
     1261 tcccaaaggg ttttcttggg agcgaagcct gatgttcgag gacgggggcg tttgcatcgc
     1321 tacaaatgac ataacactga aaggagacac tttttttaac aaagttcgat ttgatggcgt
     1381 aaactttccc ccaaatggtc ctgttatgca gaagaagact ctgaaatggg aggcatccac
     1441 tgagaaaatg tatttgcgtg atggagtgtt gacgggcgat attaccatgg ctctgctgct
     1501 taaaggagat gtccattacc gatgtgactt cagaactact tacaaatcta ggcaggaggg
     1561 tgtcaagttg ccaggatatc actttgtcga tcactgcatc agcatattga ggcatgacaa
     1621 agactacaac gaggttaagc tgtatgagca tgctgttgcc cattctggat tgccggacaa
     1681 cgtcaagtaa atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac
     1741 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1801 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1861 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     1921 tggtatggct gattatgatc ctctagatca gatctgcgaa gatacggcca cgggtgctct
     1981 tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc
     2041 tccattttct tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt
     2101 gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac
     2161 caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac
     2221 tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc
     2281 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
     2341 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
     2401 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
     2461 tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta
     2521 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     2581 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2641 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2701 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2761 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2821 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2881 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2941 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3001 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3061 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3421 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3661 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3721 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3781 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3841 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3901 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3961 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4021 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4081 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4141 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4201 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4261 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4321 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4381 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4441 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4501 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4561 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4621 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4681 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4741 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4801 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4861 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4921 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4981 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     5041 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     5101 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     5161 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     5221 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     5281 cgaaaggggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     5341 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     5401 ggtaccaaat agtaggaatt acccacctgt acaagtgctg aaaacttgga tgaataagcc
     5461 cgtttgccat tttctactgc tattttaaat cttttctgta tttgtctgca tttgtcttta
     5521 cccttgaata aatgttttag ttgttttttt tcaattatga ctgtgtttaa cagacattat
     5581 tacaataata tgtaatagta gtacacacta ttattatgta atagtacttg ttgactgtat
     5641 ttgagaactg gaccgagtga gtgttacgtc acccattcaa aatgacttac ttctggctcc
     5701 aacaaaatga agttaattca gttgccattt ttcactgtat ggacatcgcc gtgttggagc
     5761 tagacgttgc caataagcaa gaaagagccg agatgcgtcg agtctgagtc accgttccta
     5821 tggcaacccc tctaaccaat cagaagtaag cttgttggaa gtccacagcc taccacttga
     5881 aagcgggctg cacaaaatct gtcaaacgtt ttgaacgttg gatgtgagag cacatacttt
     5941 tattaaggca tctggttggt cagtttataa tatcaacaac ttgggctaca gaaaagaaaa
     6001 gttattacag aaattatgat taacaagtac atgttaaata aagattttaa tatgaatgcc
     6061 accactggag cattcatgcc atttggagct tcttcctgtt tggatcacta gaaggaggag
     6121 gtcactcatt acagttctca tatacagtcg ttggttggtt ggttggttgg tagattgatt
     6181 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     6241 gattgattgg tagtcaaaat aagaaataat ttccacagat tcattacaga aatgattaaa
     6301 tgcatacata aaaaactggg gggggggggg gatacaacaa cacacttaag tcacatttgc
     6361 ctacgtaaag aaaagtaaag aaaatcaata gctatatttt acatctcctt tttttgctgt
     6421 cttttaatta gccttgtttt gctgttatct tgattgaaac tgtaacttct tcacctgctt
     6481 cttttctttg taggttttgc cagcccagaa acaggcatgg ctgtgcaagg ccagagcacc
     6541 atgcacaatg cgctgcatgt cttcatgaac ggctcaatgt cctcagtcca gggctcagcc
     6601 aacgacccca tcttcctcct tcaccatgct ttcattgaca ggtaacaaac acgtcatgac
     6661 attagactgc acagtttttg acaaagttca tacaatctgt tgtttatagc tgctacaatt
     6721 agtgaagttt gtgaatgtac ttggatgagc agcgaaagat caattgagat caattgttag
     6781 agtttggttg ccctgcagag caaagaacaa aaaataatct ggtggcttta ctgcgtgagg
     6841 ttattattgg tggaatagaa acacaaaaca taattgcatt tatttgttta attttttatc
     6901 ttatcttaac tttcatcttg catatttgtt tcttacatca tttctagcat ctttgagcgc
     6961 tggctaagaa ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc
     7021 cacaatgacg gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc
     7081 ctgtccaaca atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca
     7141 acaccagaaa tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc
     7201 actctgttcc ac
//
