LOCUS       Exported                7760 bp ds-DNA     circular SYN 24-JUN-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCI-SEP-GluR1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 24, 2015 from SnapGene 2.7.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        138..517
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          857..989
                     /note="chimeric intron"
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1034..1052
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1201..1212
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     CDS             1343..2053
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /note="superecliptic pHluorin"
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     polyA_signal    4874..4995
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5176..5631
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5963..6067
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             6068..6928
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7099..7687
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagtgga tcccccgggc tgcaggaatt
     1081 cggcacgagc tcggctcccc ttccaagaga aacaagagaa acctcacgga aggaagggag
     1141 gaaggaaaga agcaagcaag gaactgcagg aagaaaagag ccggcagagc atcaagaaga
     1201 atcgaaggga ggggagggaa gaccaaatct atggttggac cagggcttct ttttcgccaa
     1261 tgtaaaaagg aatatgccgt acatctttgc ctttttctgc accggttttc taggtgcggt
     1321 tgtgggtgcc aatttcccta ggagtaaagg agaagaactt ttcactggag ttgtcccaat
     1381 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1441 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1501 tgttccttgg ccaacacttg tcactacttt aacttatggt gttcaatgct tttcaagata
     1561 cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1621 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1681 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1741 aaacattctt ggacacaaat tggaatacaa ctataacgat caccaggtgt acatcatggc
     1801 agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg
     1861 aggcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg ggcccgtcct
     1921 tttaccagac aaccattacc tgtttacaac ttctactctt tcgaaagatc ccaacgaaaa
     1981 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     2041 tgaactatac aaagctagcc ccaacaatat ccagataggg gggttatttc caaaccaaca
     2101 atcacaggaa catgcggctt ttaggtttgc tttgtcacaa ctcacggagc cccccaagct
     2161 gcttccccag atcgatattg tgaacatcag cgacagcttt gagatgactt accgtttctg
     2221 ttcccagttc tccaaaggag tctatgccat ctttggattt tatgaacgaa ggactgtcaa
     2281 catgctgacc tccttctgtg gggccctcca tgtgtgcttc attactccaa gttttcctgt
     2341 tgacacatcc aatcaatttg tccttcagct acgcccggaa ctacaggaag ctctcattag
     2401 cattatcgac cattacaagt ggcaaacctt tgtctacatt tatgatgctg accggggcct
     2461 gtcagtcctg cagagagtct tggatacagc cgcagagaag aactggcagg taacggctgt
     2521 caacattctg acaaccaccg aggaaggata ccggatgctc tttcaggacc tggagaagaa
     2581 aaaggagagg ctggtggtgg ttgactgtga atcagaacgc ctcaacgcca tcctgggcca
     2641 gatcgtgaag ctagaaaaga atggcatcgg gtaccactac atcctcgcca atctgggctt
     2701 catggacatt gacttaaata agttcaagga gagcggagcc aatgtgacag gtttccagct
     2761 ggtgaactac acagacacga tcccagccag aatcatgcag caatggagga caagtgactc
     2821 ccgagaccat accagggtgg actggaagag gccaaagtac acttctgctc tcacctatga
     2881 tggtgtcaag gtgatggctg aggccttcca aagcctgcgg aggcagagga ttgacatatc
     2941 ccgccggggg aatgctgggg actgtctggc taacccagct gtgccctggg gtcaagggat
     3001 cgacatccag agagccctgc agcaggtgcg cttcgaaggt ttgacaggaa atgtgcagtt
     3061 caacgagaaa gggcgccgga ccaattacac cctccacgtg atcgaaatga aacatgatgg
     3121 aatccgaaag attggttact ggaatgaaga cgataaattt gtccccgcag ccaccgacgc
     3181 tcaggctgga ggggacaact caagcgtcca gaataggacc tacatcgtca ctactatcct
     3241 cgaagatcct tacgtgatgc ttaaaaagaa tgccaaccag tttgagggca atgaccgcta
     3301 tgagggctac tgtgtggagc tggctgcaga gatcgccaag cacgtgggct actcctaccg
     3361 acttgagatt gtcagcgacg gcaaatatgg agcccgggat cccgacacaa aggcttggaa
     3421 tggcatggtg ggagaactgg tctatggaag agcagacgtg gctgtggctc ccttgaccat
     3481 aaccttggtc cgggaggaag tcatcgactt ctccaagcca ttcatgagtt tgggaatctc
     3541 cattatgatt aagaagccac agaagtccaa gccaggtgtc ttctcctttc ttgacccttt
     3601 ggcctatgag atctggatgt gtatagtgtt tgcctacatt ggagtgagcg tcgtcctctt
     3661 cctggtcagc cgtttcagcc cctacgaatg gcacagcgaa gagtttgaag agggacgaga
     3721 ccagacaacc agtgaccagt caaatgagtt tggcatattc aacagcctgt ggttctccct
     3781 gggggccttc atgcagcaag gatgtgacat ttcccccagg tccctgtccg gacgcatcgt
     3841 cggcggcgtc tggtggttct tcactttgat catcatctcc tcgtacacag ccaacctggc
     3901 tgccttcctg actgtggaga ggatggtgtc tcccattgag agtgcagagg acctggcaaa
     3961 gcagacggaa attgcttatg ggacattgga agcaggctcc actaaggagt tcttcaggag
     4021 atctaaaatc gctgtgtttg agaagatgtg gacatacatg aagtctgcag aaccatccgt
     4081 gtttgttcgg accacagagg aaggcatgat cagagtgaga aaatctaaag gcaaatacgc
     4141 ctacctcctg gagtccacca tgaatgagta tattgagcaa cgaaagccct gtgacaccat
     4201 gaaagtggga ggtaacttgg attccaaagg ctatggcatt gcgacaccca aggggtccgc
     4261 cctgagaaat ccagtaaacc tggcagtgtt aaaactgaac gagcaggggc ttttggacaa
     4321 attgaaaaac aaatggtggt acgacaaggg cgagtgcggc agcgggggag gtgactccaa
     4381 ggacaagacc agcgctttga gcctcagcaa tgtggcaggc gtgttctaca tcctgattgg
     4441 agggctggga ctggccatgc tggttgcctt aatcgagttc tgctacaaat cccgtagcga
     4501 gtcgaagcgg atgaagggtt tctgtttgat cccacagcaa tccatcaatg aagccatacg
     4561 gacatcgacc ctcccccgga acagtggggc aggagccagc ggaggaggcg gcagtggaga
     4621 gaatggccgg gtggtcagcc aggacttccc caagtccatg caatccattc cctgcatgag
     4681 tcacagttca gggatgccct tgggagccac aggattgtaa ctggagcaga caggaaaccc
     4741 ttggggagca ggctcaggct tccacagccc catcccaagc ccttcagtgc caaaaaaaaa
     4801 aaaaaaaaaa actcgagaat tcacgcgtgg tacctctaga gtcgacccgg gcggccgctt
     4861 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4921 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4981 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     5041 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     5101 ccgggctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5161 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5221 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5281 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5341 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5401 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5461 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5521 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5581 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     5641 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     5701 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5761 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5821 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5881 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5941 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6001 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6061 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6121 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6181 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6241 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6301 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6361 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6421 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6481 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6541 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6601 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6661 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6721 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6781 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6841 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6901 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6961 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7021 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7081 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7141 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7201 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7261 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7321 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7381 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7441 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7501 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7561 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7621 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7681 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7741 tcacatggct cgacagatct
//
