LOCUS       Exported                9279 bp ds-DNA     circular SYN 07-MAY-2025
DEFINITION  lentiviral vector for GATA1.
ACCESSION   .
VERSION     .
KEYWORDS    pSIN4-DDX3X-IDR1+DDX6-Delta-IDR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Elcheva I, Brok-Volchanskaya V, Kumar A, Liu P, Lee JH, Tong L, 
            Vodyanik M, Swanson S, Stewart R, Kyba M, Yakubov E, Cooke J, 
            Thomson JA, Slukvin I
  TITLE     Direct induction of haematoendothelial programs in human pluripotent
            stem cells by transcriptional regulators.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4372. doi: 10.1038/ncomms5372.
  PUBMED    25019369
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2048..2165
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2294..3472
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2525..3463
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3420..3440
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3474..3479
                     /label=EcoR1
     regulatory      3480..3488
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3489..3554
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     3540..3575
                     
     primer_bind     3554..3575
                     /label=PCDH-DDX3XIDR-F
     CDS             3555..4058
                     /label=DDX3X-IDR1(1-168)
     primer_bind     complement(4038..4073)
                     
     CDS             4059..5228
                     /codon_start=1
                     /label=DDX6-Delta-IDR
                     /translation="KGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILA
                     RAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVM
                     ATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDF
                     VQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAY
                     VTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRN
                     RVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGL
                     AINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP"
     primer_bind     4059..4085
                     /label=PCDH-DDX3XIDR1+DDX6-Delta-IDR-F
     primer_bind     complement(5211..5246)
                     
     CDS             5229..5231
                     /codon_start=1
                     /label=DDX6CDS
                     /translation=""
     misc_feature    5390..5852
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5446..5463)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5673..5692
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5885..6484
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5885..5904)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6381..6401
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6687..6920
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7071..7088)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7077..7301
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      7344..7353
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        7397..7501
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7502..8362
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7720..7739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8533..9121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9022..9041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gccacaaatg gcagtattca
     2041 tccacaattt taaaagaaag ggggggattg gggggtacag tgcaggggaa agaatagtag
     2101 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2161 attttcgggt ttattacagg gacagcagag atccactttg gatcgataag ctttgcaaag
     2221 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     2281 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2341 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     2401 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2461 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2521 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     2581 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     2641 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     2701 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     2761 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     2821 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     2881 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     2941 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     3001 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     3061 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     3121 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     3181 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     3241 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     3301 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     3361 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     3421 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattcg
     3481 ccaccatgga ctacaaggac cacgacggtg actacaagga ccacgacatc gactacaagg
     3541 acgacgacga caagagtcat gtggcagtgg aaaatgcgct cgggctggac cagcagtttg
     3601 ctggcctaga cctgaactct tcagataatc agagtggagg aagtacagcc agcaaagggc
     3661 gctatattcc tcctcattta aggaaccgag aagctactaa aggtttctac gataaagaca
     3721 gttcagggtg gagttctagc aaagataagg atgcgtatag cagttttgga tctcgtagtg
     3781 attcaagagg gaagtctagc ttcttcagtg atcgtggaag tggatcaagg ggaaggtttg
     3841 atgatcgtgg acggagtgat tacgatggca ttggcagccg tggtgacaga agtggctttg
     3901 gcaaatttga acgtggtgga aacagtcgct ggtgtgacaa atcagatgaa gatgattggt
     3961 caaaaccact cccaccaagt gaacgcttgg aacaggaact cttttctgga ggcaacactg
     4021 ggattaattt tgagaaatac gatgacattc cagttgagaa aggaaatgag tttgaagatt
     4081 actgtttgaa acgggagtta ctgatgggaa tttttgaaat gggctgggaa aagccatctc
     4141 ctattcagga ggagagcatt cccattgctt tatctggtag ggatatctta gctagagcaa
     4201 aaaatggaac aggcaagagc ggtgcctacc tcattccctt acttgaacgg ctagacctga
     4261 agaaggacaa tatacaagca atggtgattg ttcccactag agaacttgct ctacaggtca
     4321 gtcaaatttg catccaggtc agcaaacaca tgggaggggc caaagtgatg gcaaccacag
     4381 gaggaaccaa tttacgagat gacataatga ggcttgatga tacagtgcac gtggtgattg
     4441 ctacccctgg gagaatcctg gatcttatta agaaaggagt agcaaaggtt gatcatgtcc
     4501 agatgatagt attggatgag gcagataagt tgctgtcaca ggattttgtg cagataatgg
     4561 aggatattat tctcacgcta cctaaaaaca ggcagatttt actatattcc gctactttcc
     4621 ctcttagtgt acagaagttc atgaattccc atttgcagaa accctatgag attaacctga
     4681 tggaggaact aactctgaag ggagtaaccc agtactacgc atatgtaact gagcgccaaa
     4741 aagtacactg cctcaacaca cttttctcca ggcttcagat aaaccagtcg atcattttct
     4801 gtaactcctc tcagcgagtt gaattgctag ccaagaagat ttctcaactg ggttattctt
     4861 gcttctatat tcatgctaaa atgaggcagg aacatcgaaa tcgtgtattt catgatttcc
     4921 gaaatggctt atgccgcaat cttgtttgca ctgatctgtt tacccgaggt attgatatac
     4981 aagctgtgaa tgtggtaata aactttgatt tcccaaagct ggcagagacc tatctccatc
     5041 gtattggaag atcaggtcgc tttggtcatc ttggcttagc catcaacttg atcacatatg
     5101 atgatcgctt caacctgaaa agtattgagg agcagctggg aacagaaatt aaacctattc
     5161 cgagcaacat tgataagagc ctgtatgtgg cagaatacca cagcgagcct gtagaagatg
     5221 agaaacctta aacgcgtaaa ctagttcgaa ggatccgcat gcatctaggg cggccaattc
     5281 cgcccctctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     5341 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     5401 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     5461 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     5521 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     5581 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     5641 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     5701 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     5761 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     5821 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc
     5881 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     5941 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     6001 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6061 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6121 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     6181 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6241 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6301 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     6361 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     6421 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     6481 ctgataatag gcggccgctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6541 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6601 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6661 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6721 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6781 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6841 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6901 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     6961 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tataatagat ttagcaggaa
     7021 ttgaactagg agtggagcac acaggcaaag ttctagagct cgctgatcag cctcgactgt
     7081 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7141 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7201 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7261 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7321 cagctggggg cgcgcccctc gaggccgcca tggtcatagc tgtttgacgt caggtggcac
     7381 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7441 gtatccgctc acgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7501 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7561 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7621 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7681 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7741 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7801 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7861 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7921 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7981 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8041 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8101 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8161 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8221 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8281 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8341 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8401 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8461 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8521 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8581 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8641 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8701 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8761 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8821 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8881 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8941 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9001 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9061 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9121 aaacgccagc aacgcggcct ttttacggtt ctggcctttt gcacggaccg ccactgccaa
     9181 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     9241 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
