LOCUS       Exported                9840 bp ds-DNA     circular SYN 07-MAY-2025
DEFINITION  lentiviral expression of c-Myc.
ACCESSION   .
VERSION     .
KEYWORDS    LLPS-pCDH-DDX3X-IDR1+DDX6-Delta-IDR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce LA, Thompson RC, Muller S,
            Knapp S, Wang J
  TITLE     Inhibition of BET bromodomain targets genetically diverse 
            glioblastoma.
  JOURNAL   Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 
            10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12.
  PUBMED    23403638
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        226..452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             453..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2027..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2233..2436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2387..2407
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2433..2457
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     2491..2530
                     /label=XbaI-6xHis-F
     CDS             2510..2527
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2531..2566)
                     /label=LLPS-Delta-IDR-R
     primer_bind     complement(2540..2566)
                     /label=PCDH-DDX3XIDR1+DDX6-Delta-IDR-R
     CDS             2540..2557
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(2543..2566)
                     /label=pCDH-LLPS-GFP-R
     primer_bind     2552..2587
                     /label=PCDH-DDX3XIDR-F
     CDS             2567..3070
                     /label=DDX3X-IDR1(1-168)
     primer_bind     complement(3050..3085)
                     
     CDS             3071..4240
                     /codon_start=1
                     /label=DDX6-Delta-IDR
                     /translation="KGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILA
                     RAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVM
                     ATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDF
                     VQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAY
                     VTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRN
                     RVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGL
                     AINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP"
     primer_bind     3071..3097
                     /label=PCDH-DDX3XIDR1+DDX6-Delta-IDR-F
     primer_bind     complement(4213..4255)
                     /label=DDX6-WT-LLPS-R
     primer_bind     4241..4264
                     /label=pCDH-LLPS-GFP-F
     primer_bind     4243..4271
                     /label=PCDNA-GFP-F
     primer_bind     complement(4244..4257)
                     /label=LLPS-Delta-IDR-R
     CDS             4253..4972
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4298..4319)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4559..4578)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4906..4927
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4949..4989)
                     /label=NotI-GFP-R
     promoter        5014..5225
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5238..5506
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5533..6132
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5533..5552)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6029..6049
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6139..6727
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6192..6212)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6610..6621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6725..6738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             6801..7034
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7106..7227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7143..7162)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7197..7216
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7267..7402
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7329..7348
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7352..7363)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7435..7451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7435..7451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7448..7470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7459..7475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7483..7513)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7528..7549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7666..7683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7837..8425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7917..7936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8596..9456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9219..9238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9457..9561)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9629..9647
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9685..9707)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9807..9826
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa
      241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      421 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccact gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata ctagtattat
     2161 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2221 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2281 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2341 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2401 aggcgtgtac ggtgggaggt ttatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2461 tggagacgcc atccacgctg ttttgacctc catagaagat tctagaatgc atcatcacca
     2521 tcaccacgag ccaaccactc tggttccgcg tggatccgat aacgcgagtc atgtggcagt
     2581 ggaaaatgcg ctcgggctgg accagcagtt tgctggccta gacctgaact cttcagataa
     2641 tcagagtgga ggaagtacag ccagcaaagg gcgctatatt cctcctcatt taaggaaccg
     2701 agaagctact aaaggtttct acgataaaga cagttcaggg tggagttcta gcaaagataa
     2761 ggatgcgtat agcagttttg gatctcgtag tgattcaaga gggaagtcta gcttcttcag
     2821 tgatcgtgga agtggatcaa ggggaaggtt tgatgatcgt ggacggagtg attacgatgg
     2881 cattggcagc cgtggtgaca gaagtggctt tggcaaattt gaacgtggtg gaaacagtcg
     2941 ctggtgtgac aaatcagatg aagatgattg gtcaaaacca ctcccaccaa gtgaacgctt
     3001 ggaacaggaa ctcttttctg gaggcaacac tgggattaat tttgagaaat acgatgacat
     3061 tccagttgag aaaggaaatg agtttgaaga ttactgtttg aaacgggagt tactgatggg
     3121 aatttttgaa atgggctggg aaaagccatc tcctattcag gaggagagca ttcccattgc
     3181 tttatctggt agggatatct tagctagagc aaaaaatgga acaggcaaga gcggtgccta
     3241 cctcattccc ttacttgaac ggctagacct gaagaaggac aatatacaag caatggtgat
     3301 tgttcccact agagaacttg ctctacaggt cagtcaaatt tgcatccagg tcagcaaaca
     3361 catgggaggg gccaaagtga tggcaaccac aggaggaacc aatttacgag atgacataat
     3421 gaggcttgat gatacagtgc acgtggtgat tgctacccct gggagaatcc tggatcttat
     3481 taagaaagga gtagcaaagg ttgatcatgt ccagatgata gtattggatg aggcagataa
     3541 gttgctgtca caggattttg tgcagataat ggaggatatt attctcacgc tacctaaaaa
     3601 caggcagatt ttactatatt ccgctacttt ccctcttagt gtacagaagt tcatgaattc
     3661 ccatttgcag aaaccctatg agattaacct gatggaggaa ctaactctga agggagtaac
     3721 ccagtactac gcatatgtaa ctgagcgcca aaaagtacac tgcctcaaca cacttttctc
     3781 caggcttcag ataaaccagt cgatcatttt ctgtaactcc tctcagcgag ttgaattgct
     3841 agccaagaag atttctcaac tgggttattc ttgcttctat attcatgcta aaatgaggca
     3901 ggaacatcga aatcgtgtat ttcatgattt ccgaaatggc ttatgccgca atcttgtttg
     3961 cactgatctg tttacccgag gtattgatat acaagctgtg aatgtggtaa taaactttga
     4021 tttcccaaag ctggcagaga cctatctcca tcgtattgga agatcaggtc gctttggtca
     4081 tcttggctta gccatcaact tgatcacata tgatgatcgc ttcaacctga aaagtattga
     4141 ggagcagctg ggaacagaaa ttaaacctat tccgagcaac attgataaga gcctgtatgt
     4201 ggcagaatac cacagcgagc ctgtagaaga tgagaaacct gagccaacca ctatggtgag
     4261 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4321 aaacggccac aagttcagcg tgtctggcga gggcgagggc gatgccacct acggcaagct
     4381 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4441 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     4501 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4561 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     4621 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     4681 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     4741 ggcgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     4801 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4861 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4921 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aagcggccgc
     4981 gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     5041 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg
     5101 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     5161 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     5221 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag
     5281 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa
     5341 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc
     5401 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc
     5461 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg
     5521 cgcctacgct agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5581 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5641 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5701 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5761 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5821 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5881 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5941 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6001 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6061 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6121 cccggtgcct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6181 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6241 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6301 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6361 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6421 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6481 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6541 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6601 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6661 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6721 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6781 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     6841 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6901 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6961 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7021 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7081 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7141 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7201 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7261 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7321 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7381 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     7441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     9601 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     9661 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     9721 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     9781 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
//
