LOCUS       Exported                9612 bp ds-DNA     circular SYN 07-MAY-2025
DEFINITION  lentiviral expression of c-Myc.
ACCESSION   .
VERSION     .
KEYWORDS    LLPS-pCDH-DDX6-WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce LA, Thompson RC, Muller S,
            Knapp S, Wang J
  TITLE     Inhibition of BET bromodomain targets genetically diverse 
            glioblastoma.
  JOURNAL   Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 
            10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12.
  PUBMED    23403638
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        226..452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             453..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2027..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2233..2436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2387..2407
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2433..2457
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     2491..2530
                     /label=XbaI-6xHis-F
     CDS             2510..2527
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2540..2557
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(2543..2566)
                     /label=pCDH-LLPS-GFP-R
     primer_bind     2552..2595
                     /label=DDX6-WT-LLPS-F
     CDS             2567..4012
                     /codon_start=1
                     /label=DDX6-WT
                     /translation="STARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLK
                     NTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKR
                     ELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDN
                     IQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATP
                     GRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPL
                     SVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFC
                     NSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDI
                     QAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKP
                     IPSNIDKSLYVAEYHSEPVEDEKP"
     misc_feature    2567..2842
                     /label=IDR
     primer_bind     complement(3985..4027)
                     /label=DDX6-WT-LLPS-R
     primer_bind     4013..4036
                     /label=pCDH-LLPS-GFP-F
     primer_bind     4015..4043
                     /label=PCDNA-GFP-F
     CDS             4025..4744
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4070..4091)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4331..4350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4678..4699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4721..4761)
                     /label=NotI-GFP-R
     promoter        4786..4997
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5010..5278
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5305..5904
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5305..5324)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5801..5821
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5911..6499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5964..5984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6382..6393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6497..6510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             6573..6806
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6878..6999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6915..6934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6969..6988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7039..7174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7101..7120
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7124..7135)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7207..7223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7207..7223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7220..7242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7231..7247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7255..7285)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7300..7321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7438..7455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7609..8197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7689..7708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8368..9228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8991..9010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9229..9333)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9401..9419
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9457..9479)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9579..9598
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa
      241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      421 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccact gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata ctagtattat
     2161 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2221 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2281 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2341 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2401 aggcgtgtac ggtgggaggt ttatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2461 tggagacgcc atccacgctg ttttgacctc catagaagat tctagaatgc atcatcacca
     2521 tcaccacgag ccaaccactc tggttccgcg tggatccgat aacgcgagca cggccagaac
     2581 agagaaccct gttataatgg gtctgtccag tcaaaatggt cagctgagag gccctgtgaa
     2641 acccactggt ggccctggag gagggggcac acagacacag caacagatga accagctgaa
     2701 aaacaccaac acaatcaata atggcactca gcagcaagca cagagtatga ccaccactat
     2761 taaacctggt gatgactgga aaaagacttt aaaactccct ccaaaggatc taagaatcaa
     2821 aacttcggat gtgacctcca caaaaggaaa tgagtttgaa gattactgtt tgaaacggga
     2881 gttactgatg ggaatttttg aaatgggctg ggaaaagcca tctcctattc aggaggagag
     2941 cattcccatt gctttatctg gtagggatat cttagctaga gcaaaaaatg gaacaggcaa
     3001 gagcggtgcc tacctcattc ccttacttga acggctagac ctgaagaagg acaatataca
     3061 agcaatggtg attgttccca ctagagaact tgctctacag gtcagtcaaa tttgcatcca
     3121 ggtcagcaaa cacatgggag gggccaaagt gatggcaacc acaggaggaa ccaatttacg
     3181 agatgacata atgaggcttg atgatacagt gcacgtggtg attgctaccc ctgggagaat
     3241 cctggatctt attaagaaag gagtagcaaa ggttgatcat gtccagatga tagtattgga
     3301 tgaggcagat aagttgctgt cacaggattt tgtgcagata atggaggata ttattctcac
     3361 gctacctaaa aacaggcaga ttttactata ttccgctact ttccctctta gtgtacagaa
     3421 gttcatgaat tcccatttgc agaaacccta tgagattaac ctgatggagg aactaactct
     3481 gaagggagta acccagtact acgcatatgt aactgagcgc caaaaagtac actgcctcaa
     3541 cacacttttc tccaggcttc agataaacca gtcgatcatt ttctgtaact cctctcagcg
     3601 agttgaattg ctagccaaga agatttctca actgggttat tcttgcttct atattcatgc
     3661 taaaatgagg caggaacatc gaaatcgtgt atttcatgat ttccgaaatg gcttatgccg
     3721 caatcttgtt tgcactgatc tgtttacccg aggtattgat atacaagctg tgaatgtggt
     3781 aataaacttt gatttcccaa agctggcaga gacctatctc catcgtattg gaagatcagg
     3841 tcgctttggt catcttggct tagccatcaa cttgatcaca tatgatgatc gcttcaacct
     3901 gaaaagtatt gaggagcagc tgggaacaga aattaaacct attccgagca acattgataa
     3961 gagcctgtat gtggcagaat accacagcga gcctgtagaa gatgagaaac ctgagccaac
     4021 cactatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4081 ggacggcgac gtaaacggcc acaagttcag cgtgtctggc gagggcgagg gcgatgccac
     4141 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4201 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4261 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4321 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4381 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4441 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     4501 gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4561 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4621 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4681 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     4741 gtaagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     4801 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     4861 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     4921 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     4981 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     5041 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     5101 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     5161 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     5221 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     5281 agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg cctcgccacc
     5341 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     5401 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     5461 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     5521 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     5581 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     5641 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     5701 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5761 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5821 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5881 atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa
     5941 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6001 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6061 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6121 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6181 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6241 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6301 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6361 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6421 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6481 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     6541 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6601 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6781 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6841 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7021 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7081 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7141 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     7201 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8041 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8281 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8341 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8401 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8461 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8521 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8581 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8641 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8701 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8761 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8821 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8881 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8941 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9001 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9061 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9121 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9181 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9241 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9301 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9361 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     9421 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     9481 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     9541 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     9601 gagagtgcac ca
//
