LOCUS       pLRAV-JET(EcoRV)       11192 bp    DNA     circular SYN 01-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Bert Maier
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11192
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/name=RSV  promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/name=5' LTR-Delta-U3"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=Psi
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/name=Psi"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     primer_bind     1253..1271
                     /label=2647
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1253..1271
                     /label=2648
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1591..1610
                     /label=2044
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1661..1680
                     /label=2125
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1671..1690
                     /label=2068
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1701..1720
                     /label=2057
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1720..1740
                     /label=655
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1721..1740
                     /label=2059
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    1803..1920
                     /label=cPPT
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/full_name=Central polypurine tract"
                     /note="/name=cPPT"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     misc_feature    1959..2096
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     primer_bind     complement(1971..1990)
                     /label=2762
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    2097..2297
                     /label=JET promoter insert
     misc_feature    2298..5853
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     misc_feature    2793..3484
                     /label=Nr1d1 Ex1
     primer_bind     complement(2912..2928)
                     /label=741
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     2990..3006
                     /label=742
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    4535..4668
                     /label=Nr1d1 Ex2 trunc
     primer_bind     complement(4603..4622)
                     /label=1707
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    4669..4674
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=MluI"
                     /note="/official_designation=MluI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
     CDS             4675..5387
                     /label=Venus
                     /note="/Application Notes=Fast maturation and high 
                     tolerance toacidosis and Cl- compared to its predecessor 
                     YFP."
                     /note="/Brightness=156"
                     /note="/Full Name=Venus"
                     /note="/Maximum Emission (nm)=528"
                     /note="/Maximum Excitation (nm)=515"
                     /note="/References=Nat biotechnol. 20:87 (2002)"
                     /note="/Structure=Monomer (may form weak dimer)"
                     /note="/Subtype=Fluorescent reporter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Variant of yellow fluorescent protein 
                     (YFP)generated by mutagenesis; YFP is itself a variant of 
                     EGFP generated by mutagenesis"
                     /note="/name=Venus"
                     /note="/official_designation=Venus"
                     /note="/uuid=f8c7e087298f4a5984b69a6873f508a1"
     primer_bind     complement(4680..4697)
                     /label=1927
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4740..4760)
                     /label=2088
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     4903..4922
                     /label=734
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4979..4998)
                     /label=735
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     4996..5018
                     /label=2608
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5034..5053)
                     /label=1925
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5046..5065)
                     /label=2624
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5052..5071
                     /label=2625
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5210..5229
                     /label=2621
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5210..5229)
                     /label=2626
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5255..5274)
                     /label=1825
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5293..5315
                     /label=1584
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5298..5316
                     /label=729
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5308..5327
                     /label=2627
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5324..5343
                     /label=814
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5324..5343
                     /label=838
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5324..5343)
                     /label=1023
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5356..5378
                     /label=870
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5368..5387)
                     /label=2622
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    5389..5394
                     /label=XbaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XbaI"
                     /note="/official_designation=XbaI"
                     /note="/uuid=fbb65844604642bf898f6d6bc0ba2215"
     misc_feature    5398..5418
                     /label=SV40-NLS
                     /label=nonstandard type:  Tag
                     /note="/Application Notes=Directs nuclear localization of 
                     taggedprotein."
                     /note="/Full Name=SV40 nuclear localization signal"
                     /note="/References=Cell. 39:499 (1984)"
                     /note="/Subtype=Signal peptide"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Simian virus 40 nuclear localization 
                     signal"
                     /note="/name=SV40-NLS"
                     /note="/official_designation=SV40-NLS"
                     /note="/uuid=585a6ef53324445f8baca4b7b05cb10d"
     misc_feature    5560..5565
                     /label=XhoI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XhoI"
                     /note="/official_designation=XhoI"
                     /note="/uuid=a8ebc898e58a420897d3b88d510c8102"
     misc_feature    5566..5853
                     /label=mNr1d1 3'UTR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/name=mNr1d1 3'UTR"
                     /note="/official_designation=mNr1d1 3'UTR"
                     /note="/uuid=a0d214e3478544689f2633dd97c82ca9"
     primer_bind     5596..5617
                     /label=1353
                     /note="primer_bind	orchid	green	0	0"
     promoter        5878..6388
                     /label=mPGK
                     /note="/Application Notes=Medium-strength promoter."
                     /note="/Full Name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/References=Gene. 60:65 (1987); PLoS One. 5:e10611 
                     (2010)"
                     /note="/Subtype=Mammalian ubiquitous promoter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/name=mPGK"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     primer_bind     complement(5955..5974)
                     /label=1093
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6167..6190)
                     /label=1482
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    6389..6394
                     /label=kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             6395..6994
                     /label=Puro
                     /note="/Application Notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/Full Name=Puromycin resistance gene"
                     /note="/References=Gene. 62:121 (1988)"
                     /note="/Selection Drug=Puromycin"
                     /note="/Subtype=Drug selection marker"
                     /note="/Usage=Positive selection"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=Puromycin resistance gene"
                     /note="/full_name=Puromycin resistance gene"
                     /note="/name=Puro"
                     /note="/official_designation=Puro"
                     /note="/uuid=39d7413208494d9291d10b21b13ca44c"
     primer_bind     complement(6398..6415)
                     /label=1842
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6938..6948)
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6972..6991)
                     /label=914
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    7033..7630
                     /label=WPRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     LTR             7712..7946
                     /label=3' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/name=3' LTR-Delta-U3"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    8019..8153
                     /label=SV40 early pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/name=SV40 early pA"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     primer_bind     complement(8106..8126)
                     /label=792
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8335..8353)
                     /label=349
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8364..8379)
                     /label=42
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8383..8406)
                     /label=1058
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     8702..8720
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     CDS             9107..9967
                     /label=Ampicillin
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=Ampicillin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      10138..10726
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
     primer_bind     11081..11100
                     /label=1069
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     11112..11128
                     /label=43
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     11147..11167
                     /label=891
                     /note="primer_bind	orchid	green	0	0"
BASE COUNT         2623 a         3049 c         2902 g         2618 t
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcg
     2101 aattcgggcg gagttagggc ggagccaatc agcgtgcgcc gttccgaaag ttgcctttta
     2161 tggctgggcg gagaatgggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     2221 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatccctgtg
     2281 atcgtcactt gacagatatc cccgtgctat gccagtccta cccgtggggg ctggggttag
     2341 ggtgaagaag agaaaggggt tttatttaca ctgcttgtcg cccaccttgt caccatagca
     2401 aaagatgctt aagttcttga tttaagggaa atctctcctc ccagcttgtc tttctccccc
     2461 tagtcaccac taacctcagg gtgaggtcag ttctccaatc acgtgaagct ctcacgtttg
     2521 ctaggtttgc agaaagggcc ttttagcttt gatcttccac ggagcagtaa agcttgccag
     2581 gccctcccca ggaattcaca tgcccttgcc atacaaggct ttccaaacac gccaccctga
     2641 ctcttcagaa aaccccaccc cagtccactt tcagctcctc cccgctgcca gcaagcactt
     2701 ttgcaaagca gaaaaggaat aggcacactc cacctacatt gtcagtagac tacaaatccc
     2761 aacaatcctg gcggtgcgca ggcgcacaga tctcaacgtg ccggctgctg gaaaagtgtg
     2821 tcactggggc acgaggcgct ccctggaatc acatggtacc tgctccagtg ccgcgtgcgg
     2881 cccgggaacc ctgggctgct ggcgcctgcg cagagccctc tgtcccaggg aaaggctcgg
     2941 gcaaaaggcg gttgagattg gcagagtgaa atattactgc tgaggaaacg tagcagggca
     3001 cacgtctctc tttgcgtcct ggtgcctcat ttctccatca cctacttact tcctggttgc
     3061 agcttctctt cctttgggac ttttgcaccc ggagctccag attcattacc ctgcttcact
     3121 gcggagcctt caggagtggc atccggtgca ctgcagagac ccgactctcc ttgctacctt
     3181 atagccagaa ctgctgcagg ctgattcttc acacacacac tctctgctct tcccatgcaa
     3241 atcagatctc aggtgcctca gcgtcctacc cttctggagg gctgcagtat agccacccca
     3301 agaccttact gctcagtgcc tggaatcctg attgcgaact gcggggctca ctcgtctccc
     3361 tcagccattg cccagggggc gagagaggcc atcacaacct ccagtttgtg tcaaggtcca
     3421 gtttgaatga ccgctttcag ctggtgaaga catgacgacc ctggactcca ataacaacac
     3481 aggtactgag attcttatct ttgctcaggg ctcacatggc tgcagagccg ggcccacgtg
     3541 ctgcatttgt tttcattcag tagaagccgt ggccgccatc tcattggtca ttgcctcccg
     3601 gcctcgttac ataatgagct ccgcccctca agttgccgga gcaacgcttg tcgccttctc
     3661 tcctcccctc agttgtcccg gcccagcttg gttttagggc cagcctacga cccaggacgc
     3721 ccgggagggg cggggcgagg gcggggtttt ccttgcgaca gaagggaagc ggagaaggcg
     3781 gtgcttcgag ttccggggtt cctcgttggg gtgccacgtg cgaggggcac acgtggagtg
     3841 gggacgtgag gccagctgag ggccaggctc ccgccccgcc cttctccgcc tagatctggg
     3901 tgaagctggg ctgactcacc gcccccgcct ggcttctttt cctctcagaa aataacccat
     3961 cctccaaccc agccgtgaaa ccatagccgg gtaaccattc tgtgacttat tttacaggca
     4021 gggttcaccc tgaccaacct tgagctattt taaggttgcc ctgcctggtt tagtgtgtgt
     4081 cagtgggtga gcaagacatg tgcttctgtc gactcggtgg catggcatgt gtcacctcac
     4141 acacgtgtct gaggatcctc ccctttcccc actccaccca tggccggcca ggctaagtac
     4201 ttggcagagg tgtggcagac aagtggccct tctcttctgt gcataccaca ggagccgaat
     4261 cacctggccc gcaatgtaac cagagggaca gagccgctgg ctgagtggct atagagctaa
     4321 agttaccacc gggctgagaa ggaaggggaa ttcctaaatc cctagaatgg atactttgtt
     4381 tctccctttc atctcctgca actcatctga gtcttatctg tcacatttct aagtctcttg
     4441 tgactcatct ctcagaggcc agagccaggc tgaacagaga gtccttccct gcagtactct
     4501 atgagaatat gtctttgggt gttctcttat ttaggtggtg ttatcaccta cattggctct
     4561 agtggctcct ccccgagccg gaccagcccg gagtccctct acagtgacag ctccaatggc
     4621 agcttccagt ccctgactca aggttgtccc acatacttcc caccatcaac gcgtgtgagc
     4681 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4741 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4801 accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4861 accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     4921 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4981 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     5041 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     5101 tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     5161 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
     5221 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     5281 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5341 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaatc tagacctcca
     5401 aaaaagaaga gaaaggtaga agaccccttg tacaagagcc atggcttccc gccggctgtg
     5461 gctgctcagg atgatggcac gctgcccatg tcctgtgccc aggagagcgg gatggaccgt
     5521 caccctgcag cctgtgctcc tgctaggatc aatgtgtgac tcgagcccgc ccggccggcc
     5581 ttctgccgct gcccccttgt acagaatcga actctgcact tctctctcct ttacgagacg
     5641 aaaaggaaaa gcaaaccaga atcttattta tattgttata aaatattcca agatgagcct
     5701 ctggccccct gagccttctt gtaaataact cttcccccac ccccaccgcc atgctcccat
     5761 cctcccctat ttaaaccact cttgctctct ccaccctcct ctggcccctc gatttgttct
     5821 gttcctgtct caaatccaat agttcacagc tgacaagttt gtacaaaaaa gcaggctttc
     5881 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     5941 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     6001 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     6061 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     6121 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     6181 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     6241 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     6301 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     6361 tcttcctcat ctccgggcct ttcgacctgc caccatgacc gagtacaagc ccacggtgcg
     6421 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6481 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6541 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6601 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6661 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6721 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6781 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6841 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6901 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6961 cacctggtgc atgacccgca agcccggtgc ctgaacccag ctttcttgta caaagtggtg
     7021 ataatcgaat tccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7081 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7141 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7201 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7261 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7321 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7381 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7441 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7501 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7561 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7621 cccgcatcgg gaattcccgc ggttcgcttt aagaccaatg acttacaagg cagctgtaga
     7681 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7741 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7801 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7861 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7921 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7981 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     8041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     8161 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8221 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8281 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     8341 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     8401 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8461 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     8521 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8581 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8641 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8701 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8761 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8821 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8881 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8941 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     9001 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagagag
    10561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10801 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10861 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10921 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    10981 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    11041 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    11101 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
    11161 ctaaagggaa caaaagctgg agctgcaagc tt
//
