LOCUS       pL-RevErbA-JeT-m       11423 bp    DNA     circular SYN 09-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Bert Maier
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/name=RSV  promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/name=5' LTR-Delta-U3"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    521..565
                     /label=Psi
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/name=Psi"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     primer_bind     1253..1271
                     /label=2647
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1253..1271
                     /label=2648
                     /note="primer_bind	orchid	green	0	0"
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1591..1610
                     /label=2044
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1661..1680
                     /label=2125
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1671..1690
                     /label=2068
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1701..1720
                     /label=2057
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1720..1740
                     /label=655
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1721..1740
                     /label=2059
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    1803..1920
                     /label=cPPT
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/full_name=Central polypurine tract"
                     /note="/name=cPPT"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     misc_feature    1959..2096
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     primer_bind     complement(1971..1990)
                     /label=2762
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    2097..2297
                     /label=JET promoter insert
     misc_feature    2298..3484
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     misc_feature    2793..3484
                     /label=Nr1d1 Ex1
     primer_bind     complement(2912..2928)
                     /label=741
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     2990..3006
                     /label=742
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    3485..4669
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     misc_feature    4535..4668
                     /label=Nr1d1 Ex2 trunc
     primer_bind     complement(4603..4622)
                     /label=1707
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     4669..4691
                     /label=3045
     misc_feature    4669
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=MluI"
                     /note="/official_designation=MluI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
     misc_feature    4675..5619
                     /label=miRFP713
     primer_bind     complement(5599..5620)
                     /label=mi713 rv kpnI
     primer_bind     complement(5620)
                     /locus_tag="Primer 2"
                     /label=Primer 2
     CDS             5620
                     /codon_start=1
                     /locus_tag="iRFP720"
                     /product="near-infrared fluorescent protein with an
                     emission peak at 720 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP720
                     /note="derived from Rhodopseudomonas palustris RpBphP2"
                     /translation=""
     misc_feature    5621..6084
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     misc_feature    5621..5625
                     /label=XbaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XbaI"
                     /note="/official_designation=XbaI"
                     /note="/uuid=fbb65844604642bf898f6d6bc0ba2215"
     CDS             5629..5649
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5629..5649
                     /label=SV40-NLS
                     /label=nonstandard type:  Tag
                     /note="/Application Notes=Directs nuclear localization of 
                     taggedprotein."
                     /note="/Full Name=SV40 nuclear localization signal"
                     /note="/References=Cell. 39:499 (1984)"
                     /note="/Subtype=Signal peptide"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Simian virus 40 nuclear localization 
                     signal"
                     /note="/name=SV40-NLS"
                     /note="/official_designation=SV40-NLS"
                     /note="/uuid=585a6ef53324445f8baca4b7b05cb10d"
     misc_feature    5791..5796
                     /label=XhoI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XhoI"
                     /note="/official_designation=XhoI"
                     /note="/uuid=a8ebc898e58a420897d3b88d510c8102"
     misc_feature    5797..6084
                     /label=mNr1d1 3'UTR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/name=mNr1d1 3'UTR"
                     /note="/official_designation=mNr1d1 3'UTR"
                     /note="/uuid=a0d214e3478544689f2633dd97c82ca9"
     primer_bind     5827..5848
                     /label=1353
                     /note="primer_bind	orchid	green	0	0"
     protein_bind    6084..6108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6109..6619
                     /label=mPGK
                     /note="/Application Notes=Medium-strength promoter."
                     /note="/Full Name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/References=Gene. 60:65 (1987); PLoS One. 5:e10611 
                     (2010)"
                     /note="/Subtype=Mammalian ubiquitous promoter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/name=mPGK"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     promoter        6116..6615
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6186..6205)
                     /label=1093
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6398..6421)
                     /label=1482
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    6620..6625
                     /label=kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             6626..7225
                     /label=Puro
                     /note="/Application Notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/Full Name=Puromycin resistance gene"
                     /note="/References=Gene. 62:121 (1988)"
                     /note="/Selection Drug=Puromycin"
                     /note="/Subtype=Drug selection marker"
                     /note="/Usage=Positive selection"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=Puromycin resistance gene"
                     /note="/full_name=Puromycin resistance gene"
                     /note="/name=Puro"
                     /note="/official_designation=Puro"
                     /note="/uuid=39d7413208494d9291d10b21b13ca44c"
     primer_bind     complement(6629..6646)
                     /label=1842
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(7169..7179)
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(7203..7222)
                     /label=914
                     /note="primer_bind	orchid	green	0	0"
     protein_bind    complement(7226..7250)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7264..7861
                     /label=WPRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     misc_feature    7268..7856
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7739..7750)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7943..8177
                     /label=3' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/name=3' LTR-Delta-U3"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     LTR             7944..8177
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8249..8370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    8250..8384
                     /label=SV40 early pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/name=SV40 early pA"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     primer_bind     complement(8337..8357)
                     /label=792
                     /note="primer_bind	orchid	green	0	0"
     rep_origin      8410..8545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8566..8584)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8566..8584)
                     /label=349
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8594..8610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8595..8610)
                     /label=42
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8614..8637)
                     /label=1058
                     /note="primer_bind	orchid	green	0	0"
     rep_origin      8752..9207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     8933..8951
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     promoter        9233..9337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9338..10198
                     /label=Ampicillin
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=Ampicillin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      10369..10957
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
     protein_bind    11245..11266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11281..11286,11287..11304,11305..11311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     11312..11331
                     /label=1069
                     /note="primer_bind	orchid	green	0	0"
     protein_bind    11319..11335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11343..11359
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11343..11359
                     /label=43
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     11378..11398
                     /label=891
                     /note="primer_bind	orchid	green	0	0"
     promoter        11380..11398
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
BASE COUNT         2626 a         3132 c         2976 g         2689 t
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcg
     2101 aattcgggcg gagttagggc ggagccaatc agcgtgcgcc gttccgaaag ttgcctttta
     2161 tggctgggcg gagaatgggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     2221 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatccctgtg
     2281 atcgtcactt gacagatatc cccgtgctat gccagtccta cccgtggggg ctggggttag
     2341 ggtgaagaag agaaaggggt tttatttaca ctgcttgtcg cccaccttgt caccatagca
     2401 aaagatgctt aagttcttga tttaagggaa atctctcctc ccagcttgtc tttctccccc
     2461 tagtcaccac taacctcagg gtgaggtcag ttctccaatc acgtgaagct ctcacgtttg
     2521 ctaggtttgc agaaagggcc ttttagcttt gatcttccac ggagcagtaa agcttgccag
     2581 gccctcccca ggaattcaca tgcccttgcc atacaaggct ttccaaacac gccaccctga
     2641 ctcttcagaa aaccccaccc cagtccactt tcagctcctc cccgctgcca gcaagcactt
     2701 ttgcaaagca gaaaaggaat aggcacactc cacctacatt gtcagtagac tacaaatccc
     2761 aacaatcctg gcggtgcgca ggcgcacaga tctcaacgtg ccggctgctg gaaaagtgtg
     2821 tcactggggc acgaggcgct ccctggaatc acatggtacc tgctccagtg ccgcgtgcgg
     2881 cccgggaacc ctgggctgct ggcgcctgcg cagagccctc tgtcccaggg aaaggctcgg
     2941 gcaaaaggcg gttgagattg gcagagtgaa atattactgc tgaggaaacg tagcagggca
     3001 cacgtctctc tttgcgtcct ggtgcctcat ttctccatca cctacttact tcctggttgc
     3061 agcttctctt cctttgggac ttttgcaccc ggagctccag attcattacc ctgcttcact
     3121 gcggagcctt caggagtggc atccggtgca ctgcagagac ccgactctcc ttgctacctt
     3181 atagccagaa ctgctgcagg ctgattcttc acacacacac tctctgctct tcccatgcaa
     3241 atcagatctc aggtgcctca gcgtcctacc cttctggagg gctgcagtat agccacccca
     3301 agaccttact gctcagtgcc tggaatcctg attgcgaact gcggggctca ctcgtctccc
     3361 tcagccattg cccagggggc gagagaggcc atcacaacct ccagtttgtg tcaaggtcca
     3421 gtttgaatga ccgctttcag ctggtgaaga catgacgacc ctggactcca ataacaacac
     3481 aggtactgag attcttatct ttgctcaggg ctcacatggc tgcagagccg ggcccacgtg
     3541 ctgcatttgt tttcattcag tagaagccgt ggccgccatc tcattggtca ttgcctcccg
     3601 gcctcgttac ataatgagct ccgcccctca agttgccgga gcaacgcttg tcgccttctc
     3661 tcctcccctc agttgtcccg gcccagcttg gttttagggc cagcctacga cccaggacgc
     3721 ccgggagggg cggggcgagg gcggggtttt ccttgcgaca gaagggaagc ggagaaggcg
     3781 gtgcttcgag ttccggggtt cctcgttggg gtgccacgtg cgaggggcac acgtggagtg
     3841 gggacgtgag gccagctgag ggccaggctc ccgccccgcc cttctccgcc tagatctggg
     3901 tgaagctggg ctgactcacc gcccccgcct ggcttctttt cctctcagaa aataacccat
     3961 cctccaaccc agccgtgaaa ccatagccgg gtaaccattc tgtgacttat tttacaggca
     4021 gggttcaccc tgaccaacct tgagctattt taaggttgcc ctgcctggtt tagtgtgtgt
     4081 cagtgggtga gcaagacatg tgcttctgtc gactcggtgg catggcatgt gtcacctcac
     4141 acacgtgtct gaggatcctc ccctttcccc actccaccca tggccggcca ggctaagtac
     4201 ttggcagagg tgtggcagac aagtggccct tctcttctgt gcataccaca ggagccgaat
     4261 cacctggccc gcaatgtaac cagagggaca gagccgctgg ctgagtggct atagagctaa
     4321 agttaccacc gggctgagaa ggaaggggaa ttcctaaatc cctagaatgg atactttgtt
     4381 tctccctttc atctcctgca actcatctga gtcttatctg tcacatttct aagtctcttg
     4441 tgactcatct ctcagaggcc agagccaggc tgaacagaga gtccttccct gcagtactct
     4501 atgagaatat gtctttgggt gttctcttat ttaggtggtg ttatcaccta cattggctct
     4561 agtggctcct ccccgagccg gaccagcccg gagtccctct acagtgacag ctccaatggc
     4621 agcttccagt ccctgactca aggttgtccc acatacttcc caccatcaac gcgtgcggaa
     4681 ggatccgtcg ccaggcagcc tgacctcttg acctgcgacg atgagccgat ccatatcccc
     4741 ggtgccatcc aaccgcatgg actgctgctc gccctcgccg ccgacatgac gatcgttgcc
     4801 ggcagcgaca accttcccga actcaccgga ctggcgatcg gcgccctgat cggccgctct
     4861 gcggccgatg tcttcgactc ggagacgcac aaccgtctga cgatcgcctt ggccgagccc
     4921 ggggcggccg tcggagcacc gatcactgtc ggcttcacga tgcgaaagga cgcaggcttc
     4981 atcggctcct ggcatcgcca tgatcagctc atcttcctcg agctcgagcc tccccagcgg
     5041 gacgtcgccg agccgcaggc gttcttccgc cgcaccaaca gcgccatccg ccgcctgcag
     5101 gccgccgaaa ccttggaaag cgcctgcgcc gccgcggcgc aagaggtgcg gaagattacc
     5161 ggcttcgatc gggtgatgat ctatcgcttc gcctccgact tcagcggcga agtgatcgca
     5221 gaggatcggt gcgccgaggt cgagtcaaaa ctaggcctgc actatcctgc ctcaaccgtg
     5281 ccggcgcagg cccgtcggct ctataccatc aacccggtac ggatcattcc cgatatcaat
     5341 tatcggccgg tgccggtcac cccagacctc aatccggtca ccgggcggcc gattgatctt
     5401 agcttcgcca tcctgcgcag cgtctcgccc gtccatctgg aattcatgcg caacataggc
     5461 atgcacggca cgatgtcgat ctcgattttg cgcggcgagc gactgtgggg attgatcgtt
     5521 tgccatcacc gaacgccgta ctacgtcgat ctcgatggcc gccaagcctg caagagggtc
     5581 gccgagaggc tggccactca gatcggcgtg atggaagagt ctagacctcc aaaaaagaag
     5641 agaaaggtag aagacccctt gtacaagagc catggcttcc cgccggctgt ggctgctcag
     5701 gatgatggca cgctgcccat gtcctgtgcc caggagagcg ggatggaccg tcaccctgca
     5761 gcctgtgctc ctgctaggat caatgtgtga ctcgagcccg cccggccggc cttctgccgc
     5821 tgcccccttg tacagaatcg aactctgcac ttctctctcc tttacgagac gaaaaggaaa
     5881 agcaaaccag aatcttattt atattgttat aaaatattcc aagatgagcc tctggccccc
     5941 tgagccttct tgtaaataac tcttccccca cccccaccgc catgctccca tcctccccta
     6001 tttaaaccac tcttgctctc tccaccctcc tctggcccct cgatttgttc tgttcctgtc
     6061 tcaaatccaa tagttcacag ctgacaagtt tgtacaaaaa agcaggcttt ctaccgggta
     6121 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     6181 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     6241 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     6301 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     6361 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     6421 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     6481 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     6541 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     6601 tctccgggcc tttcgacctg ccaccatgac cgagtacaag cccacggtgc gcctcgccac
     6661 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     6721 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     6781 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     6841 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     6901 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     6961 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     7021 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     7081 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     7141 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     7201 catgacccgc aagcccggtg cctgaaccca gctttcttgt acaaagtggt gataatcgaa
     7261 ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7321 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7381 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7441 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7501 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7561 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7621 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     7681 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7741 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7801 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     7861 ggaattcccg cggttcgctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7921 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7981 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8041 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8101 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8161 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8221 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     8281 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8341 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     8401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8461 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8521 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     8581 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     8641 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     8701 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     8761 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8821 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8881 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8941 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     9001 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     9061 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     9121 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     9181 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     9241 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9301 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     9361 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     9421 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9481 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     9541 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     9601 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     9661 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     9721 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     9781 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     9841 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     9901 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     9961 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10021 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    10081 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    10141 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    10201 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10261 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10321 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    10381 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    10441 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    10501 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    10561 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    10621 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    10681 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    10741 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaagaga gaaaggcgga
    10801 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    10861 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    10921 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    10981 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    11041 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    11101 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    11161 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    11221 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    11281 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    11341 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
    11401 acaaaagctg gagctgcaag ctt
//
