LOCUS       pLRAV_SV40enh_Ec       11032 bp    DNA     circular SYN 14-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11032)
  AUTHORS   Bert Maier
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11032
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/name=RSV  promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/name=5' LTR-Delta-U3"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=Psi
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/name=Psi"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     primer_bind     1253..1271
                     /label=2647
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1253..1271
                     /label=2648
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1591..1610
                     /label=2044
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1661..1680
                     /label=2125
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1671..1690
                     /label=2068
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1701..1720
                     /label=2057
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1720..1740
                     /label=655
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1721..1740
                     /label=2059
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1787..1804
                     /label=3033 cPPT fw
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..2134
                     /label=SV40
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Simian virus 40 enhancer/early 
                     promoter"
                     /note="/full_name=Simian virus 40 early promoter"
                     /note="/official_designation=SV40"
                     /note="/uuid=37dc4d2f2a13436790e9f1b1d7af9c91"
     primer_bind     1959..1981
                     /label=2441
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1959..1981
                     /label=3038 SV40 por fw KpnI
     promoter        1968..2134
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2042..2061)
                     /label=2949
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(2118..2140)
                     /label=3034 SV40 Enh Rev EcoRV
     primer_bind     complement(2118..2135)
                     /label=3035 SV40 Enh Rev KpnI
     misc_feature    2138..5693
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     misc_feature    2633..3324
                     /label=Nr1d1 Ex1
     primer_bind     complement(2752..2768)
                     /label=741
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     2830..2846
                     /label=742
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    4375..4508
                     /label=Nr1d1 Ex2 trunc
     primer_bind     complement(4443..4462)
                     /label=1707
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    4509..4514
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=MluI"
                     /note="/official_designation=MluI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
     CDS             4515..5227
                     /label=Venus
                     /note="/Application Notes=Fast maturation and high 
                     tolerance toacidosis and Cl- compared to its predecessor 
                     YFP."
                     /note="/Brightness=156"
                     /note="/Full Name=Venus"
                     /note="/Maximum Emission (nm)=528"
                     /note="/Maximum Excitation (nm)=515"
                     /note="/References=Nat biotechnol. 20:87 (2002)"
                     /note="/Structure=Monomer (may form weak dimer)"
                     /note="/Subtype=Fluorescent reporter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Variant of yellow fluorescent protein 
                     (YFP)generated by mutagenesis; YFP is itself a variant of 
                     EGFP generated by mutagenesis"
                     /note="/name=Venus"
                     /note="/official_designation=Venus"
                     /note="/uuid=f8c7e087298f4a5984b69a6873f508a1"
     primer_bind     complement(4520..4537)
                     /label=1927
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4580..4600)
                     /label=2088
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     4743..4762
                     /label=734
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4819..4838)
                     /label=735
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     4836..4858
                     /label=2608
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4874..4893)
                     /label=1925
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4886..4905)
                     /label=2624
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     4892..4911
                     /label=2625
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5050..5069
                     /label=2621
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5050..5069)
                     /label=2626
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5095..5114)
                     /label=1825
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5133..5155
                     /label=1584
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5138..5156
                     /label=729
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5148..5167
                     /label=2627
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5164..5183
                     /label=814
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5164..5183
                     /label=838
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5164..5183)
                     /label=1023
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5196..5218
                     /label=870
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5208..5227)
                     /label=2622
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    5229..5234
                     /label=XbaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XbaI"
                     /note="/official_designation=XbaI"
                     /note="/uuid=fbb65844604642bf898f6d6bc0ba2215"
     misc_feature    5238..5258
                     /label=SV40-NLS
                     /label=nonstandard type:  Tag
                     /note="/Application Notes=Directs nuclear localization of 
                     taggedprotein."
                     /note="/Full Name=SV40 nuclear localization signal"
                     /note="/References=Cell. 39:499 (1984)"
                     /note="/Subtype=Signal peptide"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Simian virus 40 nuclear localization 
                     signal"
                     /note="/name=SV40-NLS"
                     /note="/official_designation=SV40-NLS"
                     /note="/uuid=585a6ef53324445f8baca4b7b05cb10d"
     misc_feature    5400..5405
                     /label=XhoI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XhoI"
                     /note="/official_designation=XhoI"
                     /note="/uuid=a8ebc898e58a420897d3b88d510c8102"
     misc_feature    5406..5693
                     /label=mNr1d1 3'UTR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/name=mNr1d1 3'UTR"
                     /note="/official_designation=mNr1d1 3'UTR"
                     /note="/uuid=a0d214e3478544689f2633dd97c82ca9"
     primer_bind     5436..5457
                     /label=1353
                     /note="primer_bind	orchid	green	0	0"
     promoter        5718..6228
                     /label=mPGK
                     /note="/Application Notes=Medium-strength promoter."
                     /note="/Full Name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/References=Gene. 60:65 (1987); PLoS One. 5:e10611 
                     (2010)"
                     /note="/Subtype=Mammalian ubiquitous promoter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/name=mPGK"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     primer_bind     complement(5795..5814)
                     /label=1093
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6007..6030)
                     /label=1482
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    6229..6234
                     /label=kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             6235..6834
                     /label=Puro
                     /note="/Application Notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/Full Name=Puromycin resistance gene"
                     /note="/References=Gene. 62:121 (1988)"
                     /note="/Selection Drug=Puromycin"
                     /note="/Subtype=Drug selection marker"
                     /note="/Usage=Positive selection"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=Puromycin resistance gene"
                     /note="/full_name=Puromycin resistance gene"
                     /note="/name=Puro"
                     /note="/official_designation=Puro"
                     /note="/uuid=39d7413208494d9291d10b21b13ca44c"
     primer_bind     complement(6238..6255)
                     /label=1842
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6778..6788)
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6812..6831)
                     /label=914
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    6873..7470
                     /label=WPRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     LTR             7552..7786
                     /label=3' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/name=3' LTR-Delta-U3"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    7859..7993
                     /label=SV40 early pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/name=SV40 early pA"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     primer_bind     complement(7946..7966)
                     /label=792
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8175..8193)
                     /label=349
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8204..8219)
                     /label=42
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8223..8246)
                     /label=1058
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     8542..8560
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     CDS             8947..9807
                     /label=Ampicillin
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=Ampicillin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      9978..10566
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
     primer_bind     10921..10940
                     /label=1069
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     10952..10968
                     /label=43
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     10987..11007
                     /label=891
                     /note="primer_bind	orchid	green	0	0"
BASE COUNT         2603 a         3016 c         2848 g         2565 t
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgct gtggaatgtg tgtcagttag
     1981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2041 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2101 tgcatctcaa ttagtcagca accatagtcc cgccgatatc cccgtgctat gccagtccta
     2161 cccgtggggg ctggggttag ggtgaagaag agaaaggggt tttatttaca ctgcttgtcg
     2221 cccaccttgt caccatagca aaagatgctt aagttcttga tttaagggaa atctctcctc
     2281 ccagcttgtc tttctccccc tagtcaccac taacctcagg gtgaggtcag ttctccaatc
     2341 acgtgaagct ctcacgtttg ctaggtttgc agaaagggcc ttttagcttt gatcttccac
     2401 ggagcagtaa agcttgccag gccctcccca ggaattcaca tgcccttgcc atacaaggct
     2461 ttccaaacac gccaccctga ctcttcagaa aaccccaccc cagtccactt tcagctcctc
     2521 cccgctgcca gcaagcactt ttgcaaagca gaaaaggaat aggcacactc cacctacatt
     2581 gtcagtagac tacaaatccc aacaatcctg gcggtgcgca ggcgcacaga tctcaacgtg
     2641 ccggctgctg gaaaagtgtg tcactggggc acgaggcgct ccctggaatc acatggtacc
     2701 tgctccagtg ccgcgtgcgg cccgggaacc ctgggctgct ggcgcctgcg cagagccctc
     2761 tgtcccaggg aaaggctcgg gcaaaaggcg gttgagattg gcagagtgaa atattactgc
     2821 tgaggaaacg tagcagggca cacgtctctc tttgcgtcct ggtgcctcat ttctccatca
     2881 cctacttact tcctggttgc agcttctctt cctttgggac ttttgcaccc ggagctccag
     2941 attcattacc ctgcttcact gcggagcctt caggagtggc atccggtgca ctgcagagac
     3001 ccgactctcc ttgctacctt atagccagaa ctgctgcagg ctgattcttc acacacacac
     3061 tctctgctct tcccatgcaa atcagatctc aggtgcctca gcgtcctacc cttctggagg
     3121 gctgcagtat agccacccca agaccttact gctcagtgcc tggaatcctg attgcgaact
     3181 gcggggctca ctcgtctccc tcagccattg cccagggggc gagagaggcc atcacaacct
     3241 ccagtttgtg tcaaggtcca gtttgaatga ccgctttcag ctggtgaaga catgacgacc
     3301 ctggactcca ataacaacac aggtactgag attcttatct ttgctcaggg ctcacatggc
     3361 tgcagagccg ggcccacgtg ctgcatttgt tttcattcag tagaagccgt ggccgccatc
     3421 tcattggtca ttgcctcccg gcctcgttac ataatgagct ccgcccctca agttgccgga
     3481 gcaacgcttg tcgccttctc tcctcccctc agttgtcccg gcccagcttg gttttagggc
     3541 cagcctacga cccaggacgc ccgggagggg cggggcgagg gcggggtttt ccttgcgaca
     3601 gaagggaagc ggagaaggcg gtgcttcgag ttccggggtt cctcgttggg gtgccacgtg
     3661 cgaggggcac acgtggagtg gggacgtgag gccagctgag ggccaggctc ccgccccgcc
     3721 cttctccgcc tagatctggg tgaagctggg ctgactcacc gcccccgcct ggcttctttt
     3781 cctctcagaa aataacccat cctccaaccc agccgtgaaa ccatagccgg gtaaccattc
     3841 tgtgacttat tttacaggca gggttcaccc tgaccaacct tgagctattt taaggttgcc
     3901 ctgcctggtt tagtgtgtgt cagtgggtga gcaagacatg tgcttctgtc gactcggtgg
     3961 catggcatgt gtcacctcac acacgtgtct gaggatcctc ccctttcccc actccaccca
     4021 tggccggcca ggctaagtac ttggcagagg tgtggcagac aagtggccct tctcttctgt
     4081 gcataccaca ggagccgaat cacctggccc gcaatgtaac cagagggaca gagccgctgg
     4141 ctgagtggct atagagctaa agttaccacc gggctgagaa ggaaggggaa ttcctaaatc
     4201 cctagaatgg atactttgtt tctccctttc atctcctgca actcatctga gtcttatctg
     4261 tcacatttct aagtctcttg tgactcatct ctcagaggcc agagccaggc tgaacagaga
     4321 gtccttccct gcagtactct atgagaatat gtctttgggt gttctcttat ttaggtggtg
     4381 ttatcaccta cattggctct agtggctcct ccccgagccg gaccagcccg gagtccctct
     4441 acagtgacag ctccaatggc agcttccagt ccctgactca aggttgtccc acatacttcc
     4501 caccatcaac gcgtgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4561 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4621 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     4681 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     4741 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4801 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4861 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4921 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     4981 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     5041 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5101 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5161 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5221 tgtacaaatc tagacctcca aaaaagaaga gaaaggtaga agaccccttg tacaagagcc
     5281 atggcttccc gccggctgtg gctgctcagg atgatggcac gctgcccatg tcctgtgccc
     5341 aggagagcgg gatggaccgt caccctgcag cctgtgctcc tgctaggatc aatgtgtgac
     5401 tcgagcccgc ccggccggcc ttctgccgct gcccccttgt acagaatcga actctgcact
     5461 tctctctcct ttacgagacg aaaaggaaaa gcaaaccaga atcttattta tattgttata
     5521 aaatattcca agatgagcct ctggccccct gagccttctt gtaaataact cttcccccac
     5581 ccccaccgcc atgctcccat cctcccctat ttaaaccact cttgctctct ccaccctcct
     5641 ctggcccctc gatttgttct gttcctgtct caaatccaat agttcacagc tgacaagttt
     5701 gtacaaaaaa gcaggctttc taccgggtag gggaggcgct tttcccaagg cagtctggag
     5761 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     5821 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     5881 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     5941 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     6001 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     6061 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     6121 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     6181 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc caccatgacc
     6241 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6301 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6361 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6421 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6481 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6541 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6601 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6661 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6721 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6781 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacccag
     6841 ctttcttgta caaagtggtg ataatcgaat tccgataatc aacctctgga ttacaaaatt
     6901 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6961 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7021 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7081 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7141 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7201 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7261 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7321 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7381 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7441 atctcccttt gggccgcctc cccgcatcgg gaattcccgc ggttcgcttt aagaccaatg
     7501 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7561 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     7621 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7681 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7741 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7801 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7981 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8041 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8101 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     8161 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     8221 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     8281 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     8341 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     8401 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     8461 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     8521 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     8581 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     8641 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     8701 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8761 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     8821 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8881 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8941 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9001 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9061 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9121 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9181 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9241 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9301 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9361 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9421 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9481 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9541 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9601 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9661 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9721 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9781 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9841 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9901 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9961 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10021 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10081 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10141 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10201 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10321 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10381 gccacgcttc ccgaagagag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10441 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10501 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10561 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10621 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    10681 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    10741 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    10801 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    10861 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    10921 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
    10981 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc tt
//
