LOCUS       pLRAV_SV40enh_Kp       11173 bp    DNA     circular SYN 14-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Bert Maier
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11173
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/name=RSV  promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/name=5' LTR-Delta-U3"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=Psi
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/name=Psi"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     primer_bind     1253..1271
                     /label=2647
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1253..1271
                     /label=2648
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1591..1610
                     /label=2044
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1661..1680
                     /label=2125
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1671..1690
                     /label=2068
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1701..1720
                     /label=2057
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1720..1740
                     /label=655
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1721..1740
                     /label=2059
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    1803..1920
                     /label=cPPT
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/full_name=Central polypurine tract"
                     /note="/name=cPPT"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     misc_feature    1959..2658
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     primer_bind     complement(1971..1990)
                     /label=2762
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    2592..2658
                     /label=Nr1d1 Ex1
     primer_bind     2654..2682
                     /label=3038 SV40 por fw KpnI
     primer_bind     2659..2682
                     /label=2441
                     /note="primer_bind	orchid	green	0	0"
     promoter        2660..2835
                     /label=SV40
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Simian virus 40 enhancer/early 
                     promoter"
                     /note="/full_name=Simian virus 40 early promoter"
                     /note="/official_designation=SV40"
                     /note="/uuid=37dc4d2f2a13436790e9f1b1d7af9c91"
     promoter        2669..2835
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2743..2762)
                     /label=2949
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(2819..2842)
                     /label=3035 SV40 Enh Rev KpnI
     primer_bind     complement(2819..2836)
                     /label=3034 SV40 Enh Rev EcoRV
     misc_feature    2841..5834
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     misc_feature    2841..3465
                     /label=Nr1d1 Ex1
     primer_bind     complement(2893..2909)
                     /label=741
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     2971..2987
                     /label=742
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    4516..4649
                     /label=Nr1d1 Ex2 trunc
     primer_bind     complement(4584..4603)
                     /label=1707
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    4650..4655
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=MluI"
                     /note="/official_designation=MluI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
     CDS             4656..5368
                     /label=Venus
                     /note="/Application Notes=Fast maturation and high 
                     tolerance toacidosis and Cl- compared to its predecessor 
                     YFP."
                     /note="/Brightness=156"
                     /note="/Full Name=Venus"
                     /note="/Maximum Emission (nm)=528"
                     /note="/Maximum Excitation (nm)=515"
                     /note="/References=Nat biotechnol. 20:87 (2002)"
                     /note="/Structure=Monomer (may form weak dimer)"
                     /note="/Subtype=Fluorescent reporter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Variant of yellow fluorescent protein 
                     (YFP)generated by mutagenesis; YFP is itself a variant of 
                     EGFP generated by mutagenesis"
                     /note="/name=Venus"
                     /note="/official_designation=Venus"
                     /note="/uuid=f8c7e087298f4a5984b69a6873f508a1"
     primer_bind     complement(4661..4678)
                     /label=1927
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4721..4741)
                     /label=2088
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     4884..4903
                     /label=734
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4960..4979)
                     /label=735
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     4977..4999
                     /label=2608
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5015..5034)
                     /label=1925
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5027..5046)
                     /label=2624
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5033..5052
                     /label=2625
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5191..5210
                     /label=2621
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5191..5210)
                     /label=2626
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5236..5255)
                     /label=1825
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5274..5296
                     /label=1584
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5279..5297
                     /label=729
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5289..5308
                     /label=2627
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5305..5324
                     /label=814
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5305..5324
                     /label=838
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5305..5324)
                     /label=1023
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5337..5359
                     /label=870
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5349..5368)
                     /label=2622
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    5370..5375
                     /label=XbaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XbaI"
                     /note="/official_designation=XbaI"
                     /note="/uuid=fbb65844604642bf898f6d6bc0ba2215"
     misc_feature    5379..5399
                     /label=SV40-NLS
                     /label=nonstandard type:  Tag
                     /note="/Application Notes=Directs nuclear localization of 
                     taggedprotein."
                     /note="/Full Name=SV40 nuclear localization signal"
                     /note="/References=Cell. 39:499 (1984)"
                     /note="/Subtype=Signal peptide"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Simian virus 40 nuclear localization 
                     signal"
                     /note="/name=SV40-NLS"
                     /note="/official_designation=SV40-NLS"
                     /note="/uuid=585a6ef53324445f8baca4b7b05cb10d"
     misc_feature    5541..5546
                     /label=XhoI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XhoI"
                     /note="/official_designation=XhoI"
                     /note="/uuid=a8ebc898e58a420897d3b88d510c8102"
     misc_feature    5547..5834
                     /label=mNr1d1 3'UTR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/name=mNr1d1 3'UTR"
                     /note="/official_designation=mNr1d1 3'UTR"
                     /note="/uuid=a0d214e3478544689f2633dd97c82ca9"
     primer_bind     5577..5598
                     /label=1353
                     /note="primer_bind	orchid	green	0	0"
     promoter        5859..6369
                     /label=mPGK
                     /note="/Application Notes=Medium-strength promoter."
                     /note="/Full Name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/References=Gene. 60:65 (1987); PLoS One. 5:e10611 
                     (2010)"
                     /note="/Subtype=Mammalian ubiquitous promoter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/name=mPGK"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     primer_bind     complement(5936..5955)
                     /label=1093
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6148..6171)
                     /label=1482
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    6370..6375
                     /label=kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             6376..6975
                     /label=Puro
                     /note="/Application Notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/Full Name=Puromycin resistance gene"
                     /note="/References=Gene. 62:121 (1988)"
                     /note="/Selection Drug=Puromycin"
                     /note="/Subtype=Drug selection marker"
                     /note="/Usage=Positive selection"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=Puromycin resistance gene"
                     /note="/full_name=Puromycin resistance gene"
                     /note="/name=Puro"
                     /note="/official_designation=Puro"
                     /note="/uuid=39d7413208494d9291d10b21b13ca44c"
     primer_bind     complement(6379..6396)
                     /label=1842
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6919..6929)
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6953..6972)
                     /label=914
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    7014..7611
                     /label=WPRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     LTR             7693..7927
                     /label=3' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/name=3' LTR-Delta-U3"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    8000..8134
                     /label=SV40 early pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/name=SV40 early pA"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     primer_bind     complement(8087..8107)
                     /label=792
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8316..8334)
                     /label=349
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8345..8360)
                     /label=42
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8364..8387)
                     /label=1058
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     8683..8701
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     CDS             9088..9948
                     /label=Ampicillin
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=Ampicillin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      10119..10707
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
     primer_bind     11062..11081
                     /label=1069
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     11093..11109
                     /label=43
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     11128..11148
                     /label=891
                     /note="primer_bind	orchid	green	0	0"
BASE COUNT         2637 a         3054 c         2879 g         2603 t
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcc
     2101 ccgtgctatg ccagtcctac ccgtgggggc tggggttagg gtgaagaaga gaaaggggtt
     2161 ttatttacac tgcttgtcgc ccaccttgtc accatagcaa aagatgctta agttcttgat
     2221 ttaagggaaa tctctcctcc cagcttgtct ttctccccct agtcaccact aacctcaggg
     2281 tgaggtcagt tctccaatca cgtgaagctc tcacgtttgc taggtttgca gaaagggcct
     2341 tttagctttg atcttccacg gagcagtaaa gcttgccagg ccctccccag gaattcacat
     2401 gcccttgcca tacaaggctt tccaaacacg ccaccctgac tcttcagaaa accccacccc
     2461 agtccacttt cagctcctcc ccgctgccag caagcacttt tgcaaagcag aaaaggaata
     2521 ggcacactcc acctacattg tcagtagact acaaatccca acaatcctgg cggtgcgcag
     2581 gcgcacagat ctcaacgtgc cggctgctgg aaaagtgtgt cactggggca cgaggcgctc
     2641 cctggaatca catggtaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     2761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2821 aaccatagtc ccgccggtac ctgctccagt gccgcgtgcg gcccgggaac cctgggctgc
     2881 tggcgcctgc gcagagccct ctgtcccagg gaaaggctcg ggcaaaaggc ggttgagatt
     2941 ggcagagtga aatattactg ctgaggaaac gtagcagggc acacgtctct ctttgcgtcc
     3001 tggtgcctca tttctccatc acctacttac ttcctggttg cagcttctct tcctttggga
     3061 cttttgcacc cggagctcca gattcattac cctgcttcac tgcggagcct tcaggagtgg
     3121 catccggtgc actgcagaga cccgactctc cttgctacct tatagccaga actgctgcag
     3181 gctgattctt cacacacaca ctctctgctc ttcccatgca aatcagatct caggtgcctc
     3241 agcgtcctac ccttctggag ggctgcagta tagccacccc aagaccttac tgctcagtgc
     3301 ctggaatcct gattgcgaac tgcggggctc actcgtctcc ctcagccatt gcccaggggg
     3361 cgagagaggc catcacaacc tccagtttgt gtcaaggtcc agtttgaatg accgctttca
     3421 gctggtgaag acatgacgac cctggactcc aataacaaca caggtactga gattcttatc
     3481 tttgctcagg gctcacatgg ctgcagagcc gggcccacgt gctgcatttg ttttcattca
     3541 gtagaagccg tggccgccat ctcattggtc attgcctccc ggcctcgtta cataatgagc
     3601 tccgcccctc aagttgccgg agcaacgctt gtcgccttct ctcctcccct cagttgtccc
     3661 ggcccagctt ggttttaggg ccagcctacg acccaggacg cccgggaggg gcggggcgag
     3721 ggcggggttt tccttgcgac agaagggaag cggagaaggc ggtgcttcga gttccggggt
     3781 tcctcgttgg ggtgccacgt gcgaggggca cacgtggagt ggggacgtga ggccagctga
     3841 gggccaggct cccgccccgc ccttctccgc ctagatctgg gtgaagctgg gctgactcac
     3901 cgcccccgcc tggcttcttt tcctctcaga aaataaccca tcctccaacc cagccgtgaa
     3961 accatagccg ggtaaccatt ctgtgactta ttttacaggc agggttcacc ctgaccaacc
     4021 ttgagctatt ttaaggttgc cctgcctggt ttagtgtgtg tcagtgggtg agcaagacat
     4081 gtgcttctgt cgactcggtg gcatggcatg tgtcacctca cacacgtgtc tgaggatcct
     4141 cccctttccc cactccaccc atggccggcc aggctaagta cttggcagag gtgtggcaga
     4201 caagtggccc ttctcttctg tgcataccac aggagccgaa tcacctggcc cgcaatgtaa
     4261 ccagagggac agagccgctg gctgagtggc tatagagcta aagttaccac cgggctgaga
     4321 aggaagggga attcctaaat ccctagaatg gatactttgt ttctcccttt catctcctgc
     4381 aactcatctg agtcttatct gtcacatttc taagtctctt gtgactcatc tctcagaggc
     4441 cagagccagg ctgaacagag agtccttccc tgcagtactc tatgagaata tgtctttggg
     4501 tgttctctta tttaggtggt gttatcacct acattggctc tagtggctcc tccccgagcc
     4561 ggaccagccc ggagtccctc tacagtgaca gctccaatgg cagcttccag tccctgactc
     4621 aaggttgtcc cacatacttc ccaccatcaa cgcgtgtgag caagggcgag gagctgttca
     4681 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4741 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca
     4801 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc
     4861 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4921 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4981 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     5041 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     5101 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     5161 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     5221 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     5281 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5341 tcactctcgg catggacgag ctgtacaaat ctagacctcc aaaaaagaag agaaaggtag
     5401 aagacccctt gtacaagagc catggcttcc cgccggctgt ggctgctcag gatgatggca
     5461 cgctgcccat gtcctgtgcc caggagagcg ggatggaccg tcaccctgca gcctgtgctc
     5521 ctgctaggat caatgtgtga ctcgagcccg cccggccggc cttctgccgc tgcccccttg
     5581 tacagaatcg aactctgcac ttctctctcc tttacgagac gaaaaggaaa agcaaaccag
     5641 aatcttattt atattgttat aaaatattcc aagatgagcc tctggccccc tgagccttct
     5701 tgtaaataac tcttccccca cccccaccgc catgctccca tcctccccta tttaaaccac
     5761 tcttgctctc tccaccctcc tctggcccct cgatttgttc tgttcctgtc tcaaatccaa
     5821 tagttcacag ctgacaagtt tgtacaaaaa agcaggcttt ctaccgggta ggggaggcgc
     5881 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     5941 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     6001 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     6061 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     6121 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     6181 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     6241 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     6301 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     6361 tttcgacctg ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6421 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6481 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6541 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6601 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6661 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6721 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6781 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6841 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6901 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6961 aagcccggtg cctgaaccca gctttcttgt acaaagtggt gataatcgaa ttccgataat
     7021 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7081 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7141 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7201 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7261 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7321 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7381 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7441 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7501 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7561 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcccg
     7621 cggttcgctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7681 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     7741 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7801 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7861 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7921 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7981 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8041 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8101 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8221 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8281 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     8341 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     8401 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     8461 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     8521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8641 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8701 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8761 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8821 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     8881 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     8941 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9001 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9061 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9121 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9181 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9241 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9301 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9361 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9421 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9481 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9541 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9601 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9661 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9721 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9781 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9841 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9901 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9961 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10021 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10081 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10141 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10201 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10261 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10321 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10381 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10441 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10501 cagcgtgagc tatgagaaag cgccacgctt cccgaagaga gaaaggcgga caggtatccg
    10561 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10621 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10681 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10741 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10801 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    10861 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    10921 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    10981 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    11041 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    11101 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
    11161 gagctgcaag ctt
//
