LOCUS       pLRAV_SV40pro_Kp       11342 bp    DNA     circular SYN 09-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Bert Maier
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11342
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/name=RSV  promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/name=5' LTR-Delta-U3"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=Psi
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/name=Psi"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     primer_bind     1253..1271
                     /label=2647
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1253..1271
                     /label=2648
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1591..1610
                     /label=2044
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1661..1680
                     /label=2125
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1671..1690
                     /label=2068
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1701..1720
                     /label=2057
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1720..1740
                     /label=655
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1721..1740
                     /label=2059
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    1803..1920
                     /label=cPPT
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/full_name=Central polypurine tract"
                     /note="/name=cPPT"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     misc_feature    1959..2658
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     primer_bind     complement(1971..1990)
                     /label=2762
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    2592..2658
                     /label=Nr1d1 Ex1
     primer_bind     2654..2682
                     /label=3038 SV40 por fw KpnI
     primer_bind     2659..2682
                     /label=2441
                     /note="primer_bind	orchid	green	0	0"
     promoter        2660..2835
                     /label=SV40
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Simian virus 40 enhancer/early 
                     promoter"
                     /note="/full_name=Simian virus 40 early promoter"
                     /note="/official_designation=SV40"
                     /note="/uuid=37dc4d2f2a13436790e9f1b1d7af9c91"
     promoter        2669..2835
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2743..2762)
                     /label=2949
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(2819..2835)
                     /label=3035 SV40 Enh Rev KpnI
     primer_bind     complement(2819..2835)
                     /label=3034 SV40 Enh Rev EcoRV
     primer_bind     2833..2852
                     /label=SV40-for
     primer_bind     2854..2870
                     /label=SV40-for
     misc_feature    3010..6003
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     misc_feature    3010..3634
                     /label=Nr1d1 Ex1
     primer_bind     complement(3062..3078)
                     /label=741
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     3140..3156
                     /label=742
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    4685..4818
                     /label=Nr1d1 Ex2 trunc
     primer_bind     complement(4753..4772)
                     /label=1707
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    4819..4824
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=MluI"
                     /note="/official_designation=MluI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
     CDS             4825..5537
                     /label=Venus
                     /note="/Application Notes=Fast maturation and high 
                     tolerance toacidosis and Cl- compared to its predecessor 
                     YFP."
                     /note="/Brightness=156"
                     /note="/Full Name=Venus"
                     /note="/Maximum Emission (nm)=528"
                     /note="/Maximum Excitation (nm)=515"
                     /note="/References=Nat biotechnol. 20:87 (2002)"
                     /note="/Structure=Monomer (may form weak dimer)"
                     /note="/Subtype=Fluorescent reporter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Variant of yellow fluorescent protein 
                     (YFP)generated by mutagenesis; YFP is itself a variant of 
                     EGFP generated by mutagenesis"
                     /note="/name=Venus"
                     /note="/official_designation=Venus"
                     /note="/uuid=f8c7e087298f4a5984b69a6873f508a1"
     primer_bind     complement(4830..4847)
                     /label=1927
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4864..4880)
                     /label=PEN1-737R
     primer_bind     complement(4890..4910)
                     /label=2088
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(4897..4915)
                     /label=EGFP-N-R+33
     primer_bind     complement(4930..4948)
                     /label=EGFP-N-rev
     primer_bind     5053..5072
                     /label=734
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5129..5148)
                     /label=735
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5146..5168
                     /label=2608
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5184..5203)
                     /label=1925
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5196..5215)
                     /label=2624
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5202..5221
                     /label=2625
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5360..5379
                     /label=2621
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5360..5379)
                     /label=2626
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5405..5424)
                     /label=1825
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5443..5465
                     /label=1584
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5448..5466
                     /label=729
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5457..5473
                     /label=EGFP-for
     primer_bind     5458..5477
                     /label=2627
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5474..5493
                     /label=814
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5474..5493
                     /label=838
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5474..5493)
                     /label=1023
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     5479..5496
                     /label=EGFP-C-for
     primer_bind     5506..5528
                     /label=870
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5518..5537)
                     /label=2622
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    5539..5544
                     /label=XbaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XbaI"
                     /note="/official_designation=XbaI"
                     /note="/uuid=fbb65844604642bf898f6d6bc0ba2215"
     misc_feature    5548..5568
                     /label=SV40-NLS
                     /label=nonstandard type:  Tag
                     /note="/Application Notes=Directs nuclear localization of 
                     taggedprotein."
                     /note="/Full Name=SV40 nuclear localization signal"
                     /note="/References=Cell. 39:499 (1984)"
                     /note="/Subtype=Signal peptide"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Simian virus 40 nuclear localization 
                     signal"
                     /note="/name=SV40-NLS"
                     /note="/official_designation=SV40-NLS"
                     /note="/uuid=585a6ef53324445f8baca4b7b05cb10d"
     misc_feature    5710..5715
                     /label=XhoI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XhoI"
                     /note="/official_designation=XhoI"
                     /note="/uuid=a8ebc898e58a420897d3b88d510c8102"
     misc_feature    5716..6003
                     /label=mNr1d1 3'UTR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/name=mNr1d1 3'UTR"
                     /note="/official_designation=mNr1d1 3'UTR"
                     /note="/uuid=a0d214e3478544689f2633dd97c82ca9"
     primer_bind     5746..5767
                     /label=1353
                     /note="primer_bind	orchid	green	0	0"
     promoter        6028..6538
                     /label=mPGK
                     /note="/Application Notes=Medium-strength promoter."
                     /note="/Full Name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/References=Gene. 60:65 (1987); PLoS One. 5:e10611 
                     (2010)"
                     /note="/Subtype=Mammalian ubiquitous promoter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/name=mPGK"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     primer_bind     complement(6105..6124)
                     /label=1093
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6317..6340)
                     /label=1482
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    6539..6544
                     /label=kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             6545..7144
                     /label=Puro
                     /note="/Application Notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/Full Name=Puromycin resistance gene"
                     /note="/References=Gene. 62:121 (1988)"
                     /note="/Selection Drug=Puromycin"
                     /note="/Subtype=Drug selection marker"
                     /note="/Usage=Positive selection"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=Puromycin resistance gene"
                     /note="/full_name=Puromycin resistance gene"
                     /note="/name=Puro"
                     /note="/official_designation=Puro"
                     /note="/uuid=39d7413208494d9291d10b21b13ca44c"
     primer_bind     complement(6548..6565)
                     /label=1842
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(7088..7098)
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(7122..7141)
                     /label=914
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    7183..7780
                     /label=WPRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     primer_bind     complement(7254..7273)
                     /label=WHV-5R
     LTR             7862..8096
                     /label=3' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/name=3' LTR-Delta-U3"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     primer_bind     complement(8168..8185)
                     /label=EGFP-C-Rev
     polyA_signal    8169..8303
                     /label=SV40 early pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/name=SV40 early pA"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     primer_bind     8205..8226
                     /label=pCI-rev
     primer_bind     complement(8256..8276)
                     /label=792
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     8259..8278
                     /label=EBV-rev
     primer_bind     8313..8332
                     /label=SV40-for
     primer_bind     8334..8350
                     /label=SV40-for
     primer_bind     complement(8484..8503)
                     /label=T7
     primer_bind     complement(8485..8503)
                     /label=349
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8514..8530)
                     /label=M13
     primer_bind     complement(8514..8529)
                     /label=42
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8533..8556)
                     /label=1058
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8533..8549)
                     /label=M13-40
     primer_bind     complement(8562..8581)
                     /label=pUCM13-52
     primer_bind     8852..8870
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     CDS             9257..10117
                     /label=Ampicillin
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=Ampicillin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     primer_bind     complement(9302..9321)
                     /label=AmpStart
     primer_bind     10048..10067
                     /label=AmpStop
     rep_origin      10288..10876
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
     primer_bind     11211..11230
                     /label=pUCM13-rev-157
     primer_bind     11231..11250
                     /label=1069
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     11245..11264
                     /label=LacOp-for
     primer_bind     11262..11278
                     /label=43
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     11297..11317
                     /label=891
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     11301..11317
                     /label=T3
BASE COUNT         2668 a         3110 c         2915 g         2649 t
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcc
     2101 ccgtgctatg ccagtcctac ccgtgggggc tggggttagg gtgaagaaga gaaaggggtt
     2161 ttatttacac tgcttgtcgc ccaccttgtc accatagcaa aagatgctta agttcttgat
     2221 ttaagggaaa tctctcctcc cagcttgtct ttctccccct agtcaccact aacctcaggg
     2281 tgaggtcagt tctccaatca cgtgaagctc tcacgtttgc taggtttgca gaaagggcct
     2341 tttagctttg atcttccacg gagcagtaaa gcttgccagg ccctccccag gaattcacat
     2401 gcccttgcca tacaaggctt tccaaacacg ccaccctgac tcttcagaaa accccacccc
     2461 agtccacttt cagctcctcc ccgctgccag caagcacttt tgcaaagcag aaaaggaata
     2521 ggcacactcc acctacattg tcagtagact acaaatccca acaatcctgg cggtgcgcag
     2581 gcgcacagat ctcaacgtgc cggctgctgg aaaagtgtgt cactggggca cgaggcgctc
     2641 cctggaatca catggtaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     2761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2821 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     2881 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     2941 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     3001 gctcggtacc tgctccagtg ccgcgtgcgg cccgggaacc ctgggctgct ggcgcctgcg
     3061 cagagccctc tgtcccaggg aaaggctcgg gcaaaaggcg gttgagattg gcagagtgaa
     3121 atattactgc tgaggaaacg tagcagggca cacgtctctc tttgcgtcct ggtgcctcat
     3181 ttctccatca cctacttact tcctggttgc agcttctctt cctttgggac ttttgcaccc
     3241 ggagctccag attcattacc ctgcttcact gcggagcctt caggagtggc atccggtgca
     3301 ctgcagagac ccgactctcc ttgctacctt atagccagaa ctgctgcagg ctgattcttc
     3361 acacacacac tctctgctct tcccatgcaa atcagatctc aggtgcctca gcgtcctacc
     3421 cttctggagg gctgcagtat agccacccca agaccttact gctcagtgcc tggaatcctg
     3481 attgcgaact gcggggctca ctcgtctccc tcagccattg cccagggggc gagagaggcc
     3541 atcacaacct ccagtttgtg tcaaggtcca gtttgaatga ccgctttcag ctggtgaaga
     3601 catgacgacc ctggactcca ataacaacac aggtactgag attcttatct ttgctcaggg
     3661 ctcacatggc tgcagagccg ggcccacgtg ctgcatttgt tttcattcag tagaagccgt
     3721 ggccgccatc tcattggtca ttgcctcccg gcctcgttac ataatgagct ccgcccctca
     3781 agttgccgga gcaacgcttg tcgccttctc tcctcccctc agttgtcccg gcccagcttg
     3841 gttttagggc cagcctacga cccaggacgc ccgggagggg cggggcgagg gcggggtttt
     3901 ccttgcgaca gaagggaagc ggagaaggcg gtgcttcgag ttccggggtt cctcgttggg
     3961 gtgccacgtg cgaggggcac acgtggagtg gggacgtgag gccagctgag ggccaggctc
     4021 ccgccccgcc cttctccgcc tagatctggg tgaagctggg ctgactcacc gcccccgcct
     4081 ggcttctttt cctctcagaa aataacccat cctccaaccc agccgtgaaa ccatagccgg
     4141 gtaaccattc tgtgacttat tttacaggca gggttcaccc tgaccaacct tgagctattt
     4201 taaggttgcc ctgcctggtt tagtgtgtgt cagtgggtga gcaagacatg tgcttctgtc
     4261 gactcggtgg catggcatgt gtcacctcac acacgtgtct gaggatcctc ccctttcccc
     4321 actccaccca tggccggcca ggctaagtac ttggcagagg tgtggcagac aagtggccct
     4381 tctcttctgt gcataccaca ggagccgaat cacctggccc gcaatgtaac cagagggaca
     4441 gagccgctgg ctgagtggct atagagctaa agttaccacc gggctgagaa ggaaggggaa
     4501 ttcctaaatc cctagaatgg atactttgtt tctccctttc atctcctgca actcatctga
     4561 gtcttatctg tcacatttct aagtctcttg tgactcatct ctcagaggcc agagccaggc
     4621 tgaacagaga gtccttccct gcagtactct atgagaatat gtctttgggt gttctcttat
     4681 ttaggtggtg ttatcaccta cattggctct agtggctcct ccccgagccg gaccagcccg
     4741 gagtccctct acagtgacag ctccaatggc agcttccagt ccctgactca aggttgtccc
     4801 acatacttcc caccatcaac gcgtgtgagc aagggcgagg agctgttcac cggggtggtg
     4861 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4921 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     4981 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
     5041 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5101 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5161 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5221 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5281 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     5341 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5401 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
     5461 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5521 atggacgagc tgtacaaatc tagacctcca aaaaagaaga gaaaggtaga agaccccttg
     5581 tacaagagcc atggcttccc gccggctgtg gctgctcagg atgatggcac gctgcccatg
     5641 tcctgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgctcc tgctaggatc
     5701 aatgtgtgac tcgagcccgc ccggccggcc ttctgccgct gcccccttgt acagaatcga
     5761 actctgcact tctctctcct ttacgagacg aaaaggaaaa gcaaaccaga atcttattta
     5821 tattgttata aaatattcca agatgagcct ctggccccct gagccttctt gtaaataact
     5881 cttcccccac ccccaccgcc atgctcccat cctcccctat ttaaaccact cttgctctct
     5941 ccaccctcct ctggcccctc gatttgttct gttcctgtct caaatccaat agttcacagc
     6001 tgacaagttt gtacaaaaaa gcaggctttc taccgggtag gggaggcgct tttcccaagg
     6061 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6121 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6181 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6241 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6301 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6361 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6421 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6481 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     6541 caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     6601 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     6661 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6721 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6781 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     6841 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6901 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6961 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7021 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7081 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7141 ctgaacccag ctttcttgta caaagtggtg ataatcgaat tccgataatc aacctctgga
     7201 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7261 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7321 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7381 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7441 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7501 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7561 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7621 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7681 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7741 gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattcccgc ggttcgcttt
     7801 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7861 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     7921 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7981 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8041 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8101 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8161 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8281 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     8341 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8401 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8461 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     8521 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     8581 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     8641 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     8701 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8761 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8821 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8881 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8941 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9001 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     9061 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     9121 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9481 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9781 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    10021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    10081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    10141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10681 atgagaaagc gccacgcttc ccgaagagag aaaggcggac aggtatccgg taagcggcag
    10741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    11041 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    11101 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    11161 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    11221 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
    11281 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
    11341 tt
//
