LOCUS       pL-RevErbA-SV40(       11002 bp    DNA     circular SYN 09-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Bert Maier
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/name=RSV  promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/name=5' LTR-Delta-U3"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=Psi
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/name=Psi"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     primer_bind     1253..1271
                     /label=2647
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1253..1271
                     /label=2648
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1591..1610
                     /label=2044
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1661..1680
                     /label=2125
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1671..1690
                     /label=2068
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1701..1720
                     /label=2057
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1720..1740
                     /label=655
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1721..1740
                     /label=2059
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1787..1804
                     /label=3033 cPPT fw
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..2134
                     /label=SV40
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Simian virus 40 enhancer/early 
                     promoter"
                     /note="/full_name=Simian virus 40 early promoter"
                     /note="/official_designation=SV40"
                     /note="/uuid=37dc4d2f2a13436790e9f1b1d7af9c91"
     primer_bind     1959..1981
                     /label=2441
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     1959..1981
                     /label=3038 SV40 por fw KpnI
     promoter        1968..2134
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2042..2061)
                     /label=2949
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(2118..2140)
                     /label=3034 SV40 Enh Rev EcoRV
     primer_bind     complement(2118..2135)
                     /label=3035 SV40 Enh Rev KpnI
     misc_feature    2138..5663
                     /label={RAV}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={RAV}"
                     /note="/official_designation={RAV}"
                     /note="/uuid=402822e70c9146829a2226340a4f2835"
     misc_feature    2633..3324
                     /label=Nr1d1 Ex1
     primer_bind     complement(2752..2768)
                     /label=741
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     2830..2846
                     /label=742
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    4375..4508
                     /label=Nr1d1 Ex2 trunc
     primer_bind     complement(4443..4462)
                     /label=1707
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     4509..4538
                     /label=3039
     misc_feature    4509..4514
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=MluI"
                     /note="/official_designation=MluI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
     CDS             4515..5198
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="DSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGTV
                     SVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKMA
                     LRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHST
                     GGSGGS"
     regulatory      4515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4642..4660)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4979..4988)
                     /label=2624
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5183..5204)
                     /label=3040
     misc_feature    5199..5204
                     /label=XbaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XbaI"
                     /note="/official_designation=XbaI"
                     /note="/uuid=fbb65844604642bf898f6d6bc0ba2215"
     misc_feature    5208..5228
                     /label=SV40-NLS
                     /label=nonstandard type:  Tag
                     /note="/Application Notes=Directs nuclear localization of 
                     taggedprotein."
                     /note="/Full Name=SV40 nuclear localization signal"
                     /note="/References=Cell. 39:499 (1984)"
                     /note="/Subtype=Signal peptide"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/description=Simian virus 40 nuclear localization 
                     signal"
                     /note="/name=SV40-NLS"
                     /note="/official_designation=SV40-NLS"
                     /note="/uuid=585a6ef53324445f8baca4b7b05cb10d"
     misc_feature    5370..5375
                     /label=XhoI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/direction=NONE"
                     /note="/name=XhoI"
                     /note="/official_designation=XhoI"
                     /note="/uuid=a8ebc898e58a420897d3b88d510c8102"
     misc_feature    5376..5663
                     /label=mNr1d1 3'UTR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/name=mNr1d1 3'UTR"
                     /note="/official_designation=mNr1d1 3'UTR"
                     /note="/uuid=a0d214e3478544689f2633dd97c82ca9"
     primer_bind     5406..5427
                     /label=1353
                     /note="primer_bind	orchid	green	0	0"
     promoter        5688..6198
                     /label=mPGK
                     /note="/Application Notes=Medium-strength promoter."
                     /note="/Full Name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/References=Gene. 60:65 (1987); PLoS One. 5:e10611 
                     (2010)"
                     /note="/Subtype=Mammalian ubiquitous promoter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/name=mPGK"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     primer_bind     complement(5765..5784)
                     /label=1093
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(5977..6000)
                     /label=1482
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    6199..6204
                     /label=kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             6205..6804
                     /label=Puro
                     /note="/Application Notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/Full Name=Puromycin resistance gene"
                     /note="/References=Gene. 62:121 (1988)"
                     /note="/Selection Drug=Puromycin"
                     /note="/Subtype=Drug selection marker"
                     /note="/Usage=Positive selection"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=Puromycin resistance gene"
                     /note="/full_name=Puromycin resistance gene"
                     /note="/name=Puro"
                     /note="/official_designation=Puro"
                     /note="/uuid=39d7413208494d9291d10b21b13ca44c"
     primer_bind     complement(6208..6225)
                     /label=1842
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6748..6758)
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(6782..6801)
                     /label=914
                     /note="primer_bind	orchid	green	0	0"
     misc_feature    6843..7440
                     /label=WPRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     LTR             7522..7756
                     /label=3' LTR-Delta-U3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/name=3' LTR-Delta-U3"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    7829..7963
                     /label=SV40 early pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/name=SV40 early pA"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     primer_bind     complement(7916..7936)
                     /label=792
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8145..8163)
                     /label=349
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8174..8189)
                     /label=42
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     complement(8193..8216)
                     /label=1058
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     8512..8530
                     /label=700
                     /note="primer_bind	orchid	green	0	0"
     CDS             8917..9777
                     /label=Ampicillin
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=Ampicillin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      9948..10536
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
     primer_bind     10891..10910
                     /label=1069
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     10922..10938
                     /label=43
                     /note="primer_bind	orchid	green	0	0"
     primer_bind     10957..10977
                     /label=891
                     /note="primer_bind	orchid	green	0	0"
BASE COUNT         2579 a         2999 c         2854 g         2570 t
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgct gtggaatgtg tgtcagttag
     1981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2041 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2101 tgcatctcaa ttagtcagca accatagtcc cgccgatatc cccgtgctat gccagtccta
     2161 cccgtggggg ctggggttag ggtgaagaag agaaaggggt tttatttaca ctgcttgtcg
     2221 cccaccttgt caccatagca aaagatgctt aagttcttga tttaagggaa atctctcctc
     2281 ccagcttgtc tttctccccc tagtcaccac taacctcagg gtgaggtcag ttctccaatc
     2341 acgtgaagct ctcacgtttg ctaggtttgc agaaagggcc ttttagcttt gatcttccac
     2401 ggagcagtaa agcttgccag gccctcccca ggaattcaca tgcccttgcc atacaaggct
     2461 ttccaaacac gccaccctga ctcttcagaa aaccccaccc cagtccactt tcagctcctc
     2521 cccgctgcca gcaagcactt ttgcaaagca gaaaaggaat aggcacactc cacctacatt
     2581 gtcagtagac tacaaatccc aacaatcctg gcggtgcgca ggcgcacaga tctcaacgtg
     2641 ccggctgctg gaaaagtgtg tcactggggc acgaggcgct ccctggaatc acatggtacc
     2701 tgctccagtg ccgcgtgcgg cccgggaacc ctgggctgct ggcgcctgcg cagagccctc
     2761 tgtcccaggg aaaggctcgg gcaaaaggcg gttgagattg gcagagtgaa atattactgc
     2821 tgaggaaacg tagcagggca cacgtctctc tttgcgtcct ggtgcctcat ttctccatca
     2881 cctacttact tcctggttgc agcttctctt cctttgggac ttttgcaccc ggagctccag
     2941 attcattacc ctgcttcact gcggagcctt caggagtggc atccggtgca ctgcagagac
     3001 ccgactctcc ttgctacctt atagccagaa ctgctgcagg ctgattcttc acacacacac
     3061 tctctgctct tcccatgcaa atcagatctc aggtgcctca gcgtcctacc cttctggagg
     3121 gctgcagtat agccacccca agaccttact gctcagtgcc tggaatcctg attgcgaact
     3181 gcggggctca ctcgtctccc tcagccattg cccagggggc gagagaggcc atcacaacct
     3241 ccagtttgtg tcaaggtcca gtttgaatga ccgctttcag ctggtgaaga catgacgacc
     3301 ctggactcca ataacaacac aggtactgag attcttatct ttgctcaggg ctcacatggc
     3361 tgcagagccg ggcccacgtg ctgcatttgt tttcattcag tagaagccgt ggccgccatc
     3421 tcattggtca ttgcctcccg gcctcgttac ataatgagct ccgcccctca agttgccgga
     3481 gcaacgcttg tcgccttctc tcctcccctc agttgtcccg gcccagcttg gttttagggc
     3541 cagcctacga cccaggacgc ccgggagggg cggggcgagg gcggggtttt ccttgcgaca
     3601 gaagggaagc ggagaaggcg gtgcttcgag ttccggggtt cctcgttggg gtgccacgtg
     3661 cgaggggcac acgtggagtg gggacgtgag gccagctgag ggccaggctc ccgccccgcc
     3721 cttctccgcc tagatctggg tgaagctggg ctgactcacc gcccccgcct ggcttctttt
     3781 cctctcagaa aataacccat cctccaaccc agccgtgaaa ccatagccgg gtaaccattc
     3841 tgtgacttat tttacaggca gggttcaccc tgaccaacct tgagctattt taaggttgcc
     3901 ctgcctggtt tagtgtgtgt cagtgggtga gcaagacatg tgcttctgtc gactcggtgg
     3961 catggcatgt gtcacctcac acacgtgtct gaggatcctc ccctttcccc actccaccca
     4021 tggccggcca ggctaagtac ttggcagagg tgtggcagac aagtggccct tctcttctgt
     4081 gcataccaca ggagccgaat cacctggccc gcaatgtaac cagagggaca gagccgctgg
     4141 ctgagtggct atagagctaa agttaccacc gggctgagaa ggaaggggaa ttcctaaatc
     4201 cctagaatgg atactttgtt tctccctttc atctcctgca actcatctga gtcttatctg
     4261 tcacatttct aagtctcttg tgactcatct ctcagaggcc agagccaggc tgaacagaga
     4321 gtccttccct gcagtactct atgagaatat gtctttgggt gttctcttat ttaggtggtg
     4381 ttatcaccta cattggctct agtggctcct ccccgagccg gaccagcccg gagtccctct
     4441 acagtgacag ctccaatggc agcttccagt ccctgactca aggttgtccc acatacttcc
     4501 caccatcaac gcgtgatagc accgaggcag tgatcaagga gttcatgcgg ttcaaggtgc
     4561 acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4621 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttct
     4681 cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcatc aagcaccccg
     4741 ccgacatccc cgactactgg aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga
     4801 tgatcttcga ggacggcggc accgtgtccg tgacccagga cacctccctg gaggacggca
     4861 ccctgatcta caaggtgaag ctccgcggcg gcaacttccc tcctgacggc cccgtaatgc
     4921 agaagaggac aatgggctgg gaagcatcca ccgagcggtt gtaccccgag gacgtcgtgc
     4981 tgaagggcga cattaagatg gccctgcgcc tgaaggacgg cggccgctac ctggcggact
     5041 tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc cggcgccttc aacatcgacc
     5101 gcaagttgga catcacctcc cacaacgagg actacaccgt ggtggaacag tacgaacgct
     5161 ccgtggcccg ccactccacc ggcggctccg gtggctcctc tagacctcca aaaaagaaga
     5221 gaaaggtaga agaccccttg tacaagagcc atggcttccc gccggctgtg gctgctcagg
     5281 atgatggcac gctgcccatg tcctgtgccc aggagagcgg gatggaccgt caccctgcag
     5341 cctgtgctcc tgctaggatc aatgtgtgac tcgagcccgc ccggccggcc ttctgccgct
     5401 gcccccttgt acagaatcga actctgcact tctctctcct ttacgagacg aaaaggaaaa
     5461 gcaaaccaga atcttattta tattgttata aaatattcca agatgagcct ctggccccct
     5521 gagccttctt gtaaataact cttcccccac ccccaccgcc atgctcccat cctcccctat
     5581 ttaaaccact cttgctctct ccaccctcct ctggcccctc gatttgttct gttcctgtct
     5641 caaatccaat agttcacagc tgacaagttt gtacaaaaaa gcaggctttc taccgggtag
     5701 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     5761 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     5821 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     5881 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     5941 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     6001 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     6061 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     6121 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     6181 ctccgggcct ttcgacctgc caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     6241 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6301 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6361 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6421 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6481 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6541 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6601 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6661 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6721 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6781 atgacccgca agcccggtgc ctgaacccag ctttcttgta caaagtggtg ataatcgaat
     6841 tccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7381 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg
     7441 gaattcccgc ggttcgcttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7501 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     7561 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7621 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7681 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7741 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7801 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7921 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7981 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8041 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8101 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     8161 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8221 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8281 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     8341 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8401 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8461 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8521 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8581 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8641 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8701 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8761 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     8821 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8881 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8941 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9001 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9061 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9121 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9181 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9241 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9301 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9361 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9421 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9481 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9541 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9601 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9661 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9721 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9781 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9841 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9901 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10081 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagagag aaaggcggac
    10381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10501 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10561 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10621 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10681 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    10741 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    10801 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10861 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    10921 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
    10981 caaaagctgg agctgcaagc tt
//
