LOCUS       pIDTSMART-MbPylRS-4xHTS 8436 bp ds-DNA     circular     21-OCT-2023
DEFINITION  .
COMMENT     ^^
FEATURES             Location/Qualifiers
     misc_feature    5..22
                     /label="M13F -21"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     PCR_primer      191..191
                     /label="aataatGCTAGCAAAAAATACCCGGAGCGGGACTTGAACC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    192..209
                     /label="synthetase cassette"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(210..217)
                     /label="ITR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    210..350
                     /label="ITR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(390..868)
                     /label="Poly-A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(901..1641)
                     /label="GFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(901..1641)
                     /label="Translation 1641-901"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKYSDLELK*"
     misc_feature    complement(1664..2156)
                     /label="Chicken beta globin intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2164..2822)
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2831..2971
                     /label="ITR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3001..3260
                     /label="U6"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3261..3261
                     /label="Acceptor-Mut-R annealing TM = 67"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3261..3329
                     /label="MmPylT-c"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3278..3309
                     /label="Acceptor overlap TM = 69"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(3306..3322)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(3306..3322)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(3306..3322)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(3306..3322)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3328..3329
                     /label="Acceptor-Mut-F annealing TM = 69"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3330..3340)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(3330..3340)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3330..3340)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(3330..3340)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGAATC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3348..3607
                     /label="U6"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3608..3608
                     /label="Acceptor-Mut-R annealing TM = 67"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3608..3676
                     /label="MmPylT-c"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3625..3656
                     /label="Acceptor overlap TM = 69"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(3653..3669)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(3653..3669)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(3653..3669)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(3653..3669)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(3653..3687)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(3653..3687)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGAATC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(3653..3687)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(3653..3687)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3675..3676
                     /label="Acceptor-Mut-F annealing TM = 69"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3695..3954
                     /label="U6"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3955..3955
                     /label="Acceptor-Mut-R annealing TM = 67"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3955..4023
                     /label="MmPylT-c"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3972..4003
                     /label="Acceptor overlap TM = 69"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(4000..4016)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(4000..4016)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(4000..4016)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(4000..4016)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4022..4023
                     /label="Acceptor-Mut-F annealing TM = 69"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4024..4034)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4024..4034)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGAATC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(4024..4034)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(4024..4034)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4042..4301
                     /label="U6"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4302..4302
                     /label="Acceptor-Mut-R annealing TM = 67"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4302..4370
                     /label="MmPylT-c"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4319..4350
                     /label="Acceptor overlap TM = 69"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(4347..4363)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4347..4363)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(4347..4363)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(4347..4363)
                     /label="MmPytR-Nhe-R - aataatgctagcAAAAAACGGAAACCCCGGGA..."
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4369..4370
                     /label="Acceptor-Mut-F annealing TM = 69"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4380..4947
                     /label="CMV"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     PCR_primer      4897..4917
                     /label="CGCAAATGGGCGGTAGGCGTG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4991..5007
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5035..6294
                     /label="MbPylRS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    6301..6301
                     /label="PuromycinR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     PCR_primer      complement(6359..6376)
                     /label="TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(6365..6589)
                     /label="Bgh polyA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6936..7754
                     /label="KanR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    7814..8401
                     /label="Ori Rep"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 CCCGTGTAAA ACGACGGCCA GTTTATCTAG TCAGCTTGAT TCTAGCTGAT CGTGGACCGG
       61 AAGGTGAGCC AGTGAGTTGA TTGCAGTCCA GTTACGCTGG AGTCTGAGGC TCGTCCTGAA
      121 TGATATGCGa CCGCCGGAGG GTTGCGTTTG AGACGGGCGA CAGATCCAGT CGCGCTGCTC
      181 TCGTCGATCC GctaggGCGG CCGCaaataC CTGCAGGCAG CTGCGCGCTC GCTCGCTCAC
      241 TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT GGTCGCCCGG CCTCAGTGAG
      301 CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT AGGGGTTCCT GCGGCCGCTC
      361 GGTCCGCACG TGGTTACCTA CAAAATCAGA AGGACAGGGA AGGGAGCAGT GGTTCACGCC
      421 TGTAATCCCA GCAATTTGGG AGGCCAAGGT GGGTAGATCA CCTGAGATTA GGAGTTGGAG
      481 ACCAGCCTGG CCAATATGGT GAAACCCCGT CTCTACCAAA AAAACAAAAA TTAGCTGAGC
      541 CTGGTCATGC ATGCCTGGAA TCCCAACAAC TCGGGAGGCT GAGGCAGGAG AATCGCTTGA
      601 ACCCAGGAGG CGGAGATTGC AGTGAGCCAA GATTGTGCCA CTGCACTCCA GCTTGGTTCC
      661 CAATAGACCC CGCAGGCCCT ACAGGTTGTC TTCCCAACTT GCCCCTTGCT CCATACCACC
      721 CCCCTCCACC CCATAATATT ATAGAAGGAC ACCTAGTCAG ACAAAATGAT GCAACTTAAT
      781 TTTATTAGGA CAAGGCTGGT GGGCACTGGA GTGGCAACTT CCAGGGCCAG GAGAGGCACT
      841 GGGGAGGGGT CACAGGGATG CCACCCGTAG ATCTCTCGAG CAGCGCTGCT CGAGGCAAGC
      901 TTACTTGAGC TCGAGATCTG AGTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC
      961 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG
     1021 GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG
     1081 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT
     1141 CTTGAAGTTC ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT
     1201 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC
     1261 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT
     1321 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC
     1381 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC
     1441 GTAGGTCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT
     1501 GAACTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA
     1561 CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG
     1621 CTCCTCGCCC TTGCTCACCA TTCAGAATTC AATCGATGTT CGAATCCCAA TTCTTTGCCA
     1681 AAGTGATGGG CCAGCACACA GACCAGCACG TTGCCCAGGA GCTGTGGGAG GAAGATAAGA
     1741 GGTATGAACA TGATTAGCAA AAGGGCCTAG CTTGGACTCA GAATAATCCA GCCTTATCCC
     1801 AACCATAAAA TAAAAGCAGA ATGGTAGCTG GATTGTAGCT GCTATTAGCA ATATGAAACC
     1861 TCTTACATCA GTTACAATTT ATATGCAGAA ATATTTATAT GCAGAAATAT TGCTATTGCC
     1921 TTAACCCAGA AATTATCACT GTTATTCTTT AGAATGGTGC AAAGAGGCAT GATACATTGT
     1981 ATCATTATTG CCCTGAAAGA AAGAGATTAG GGAAAGTATT AGAAATAAGA TAAACAAAAA
     2041 AGTATATTAA AAGAAGAAAG CATTTTTTGT GGGCCTATAG ACTCTATAGG CGGTACTTAC
     2101 GTCACTCTTG GCACGGGGAA TCCGCGTTCC AATGCACCGT TCCCGGCCGG GATTCGAATC
     2161 CGCGGAGGCT GGATCGGTCC CGGTGTCTTC TATGGAGGTC AAAACAGCGT GGATGGCGTC
     2221 TCCAGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTTAT ATAGACCTCC CACCGTACAC
     2281 GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG ACATTTTGGA AAGTCCCGTT
     2341 GATTTTGGTG CAAAACAAAC TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG
     2401 TGAGTCAAAC CGCTATCCAC GCCCATTGAT GTACTGCCAA AACCGCATCA CCATGGTAAT
     2461 AGCGATGACT AATACGTAGA TGTACTGCCA AGTAGGAAAG TCCCATAAGG TCATGTACTG
     2521 GGCATAATGC CAGGCGGGCC ATTTACCGTC ATTGACGTCA ATAGGGGGCG TACTTGGCAT
     2581 ATGATACACT TGATGTACTG CCAAGTGGGC AGTTTACCGT AAATACTCCA CCCATTGACG
     2641 TCAATGGAAA GTCCCTATTG GCGTTACTAT GGGAACATAC GTCATTATTG ACGTCAATGG
     2701 GCGGGGGTCG TTGGGCGGTC AGCCAGGCGG GCCATTTACC GTAAGTTATG TAACGCGGAA
     2761 CTCCATATAT GGGCTATGAA CTAATGACCC CGTAATTGAT TACTATTAAT AACTAGACGC
     2821 GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG
     2881 CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA
     2941 GTGAGCGAGC GAGCGCGCAG CTGCCTGCAG Gctcgatccg ctcgcacccc taggggtacc
     3001 tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     3061 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
     3121 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     3181 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3241 tgtggaaagg acgaaacacc gggcggctga tcatgtagat cgaacggact CTAaatccgt
     3301 tcagccgggt tagattcccg ggctgcccgt tttttgctag gggtacctcg ggcaggaaga
     3361 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     3421 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     3481 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     3541 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     3601 aaacaccggg cggctgatca tgtagatcga acggactCTA aatccgttca gccgggttag
     3661 attcccgggc tgcccgtttt ttgctagggg tacctcgggc aggaagaggg cctatttccc
     3721 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     3781 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     3841 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     3901 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggcgg
     3961 ctgatcatgt agatcgaacg gactCTAaat ccgttcagcc gggttagatt cccgggctgc
     4021 ccgttttttg ctaggggtac ctcgggcagg aagagggcct atttcccatg attccttcat
     4081 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     4141 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     4201 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     4261 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgggcggctg atcatgtaga
     4321 tcgaacggac tCTAaatccg ttcagccggg ttagattccc gggctgcccg ttttttgcta
     4381 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     4441 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     4501 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     4561 gggtggaGta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     4621 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     4681 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     4741 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     4801 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     4861 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     4921 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
     4981 ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgc caccATGGAT
     5041 AAAAAACCAT TAGATGTTTT AATATCTGCG ACCGGGCTCT GGATGTCCAG GACTGGCACG
     5101 CTCCACAAAA TCAAGCACCA TGAGGTCTCA AGAAGTAAAA TATACATTGA AATGGCGTGT
     5161 GGAGACCATC TTGTTGTGAA TAATTCCAGG AGTTGTAGAA CAGCCAGAGC ATTCAGACAT
     5221 CATAAGTACA GAAAAACCTG CAAACGATGT AGGGTTTCGG ACGAGGATAT CAATAATTTT
     5281 CTCACAAGAT CAACCGAAAG CAAAAACAGT GTGAAAGTTA GGGTAGTTTC TGCTCCAAAG
     5341 GTCAAAAAAG CTATGCCGAA ATCAGTTTCA AGGGCTCCGA AGCCTCTGGA AAATTCTGTT
     5401 TCTGCAAAGG CATCAACGAA CACATCCAGA TCTGTACCTT CGCCTGCAAA ATCAACTCCA
     5461 AATTCGTCTG TTCCCGCATC GGCTCCTGCT CCTTCACTTA CAAGAAGCCA GCTTGATAGG
     5521 GTTGAGGCTC TCTTAAGTCC AGAGGATAAA ATTTCTCTAA ATATGGCAAA GCCTTTCAGG
     5581 GAACTTGAGC CTGAACTTGT GACAAGAAGA AAAAACGATT TTCAGCGGCT CTATACCAAT
     5641 GATAGAGAAG ACTACCTCGG TAAACTCGAA CGTGATATTA CGAAATTTTT CGTAGACCGG
     5701 GGTTTTCTGG AGATAAAGTC TCCTATCCTT ATTCCGGCGG AATACGTGGA GAGAATGGGT
     5761 ATTAATAATG ATACTGAACT TTCAAAACAG ATCTTCCGGG TGGATAAAAA TCTCTGCTTG
     5821 AGGCCAATGC TTGCCCCGAC TCTTTACAAC TATCTGCGAA AACTCGATAG GATTTTACCA
     5881 GGCCCAATAA AAATTTTCGA AGTCGGACCT TGTTACCGGA AAGAGTCTGA CGGCAAAGAG
     5941 CACCTGGAAG AATTTACTAT GGTGAACTTC TGTCAGATGG GTTCGGGATG TACTCGGGAA
     6001 AATCTTGAAG CTCTCATCAA AGAGTTTCTG GACTATCTGG AAATCGACTT CGAAATCGTA
     6061 GGAGATTCCT GTATGGTCTt TGGGGATACT CTTGATATAA TGCACGGGGA CCTGGAGCTT
     6121 TCTTCGGCAG TCGTCGGGCC AGTTTCTCTT GATAGAGAAT GGGGTATTGA CAAACCATGG
     6181 ATAGGTGCAG GTTTTGGTCT TGAACGCTTG CTCAAGGTTA TGCACGGCTT TAAAAACATT
     6241 AAGAGGGCAT CAAGGTCCGA ATCTTACTAT AATGGGATTT CAACCAATCT GTAAgaattc
     6301 aacgcgttaa gtcgacttta actcgagtct agagggcccg tttaaacccg ctgatcagcc
     6361 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6421 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6481 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6541 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6601 gaaagaacCC TAGGGGTGCG AGCGGATCGA GCAGTGTCGA TCACTACTGG ACCGCGAGCT
     6661 GTGCTGCGAC cCGTGATCTT ACGGCATTAT ACGTATGATC GGTCCACGAT CAGCTAGATT
     6721 ATCTAGTCAG CTTGATGTCA TAGCTGTTTC CTGAGGCTCA ATACTGACCA TTTAAATCAT
     6781 ACCTGACCTC CATAGCAGAA AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGATCGGC
     6841 ACGTAAGAGG TTCCAACTTT CACCATAATG AAATAAGATC ACTACCGGGC GTATTTTTTG
     6901 AGTTATCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGAG CCATATTCAA CGGGAAACGT
     6961 CTTGCTTGAA GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG
     7021 CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG ATTGTATGGG AAGCCCGATG
     7081 CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA
     7141 TGGTCAGGCT AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC
     7201 GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC AGGGAAAACA GCATTCCAGG
     7261 TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC
     7321 GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA CGGCGATCGC GTATTTCGTC
     7381 TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGG TGCGAGTGAT TTTGATGACG
     7441 AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTC TTGCCATTCT
     7501 CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG
     7561 GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC
     7621 TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC
     7681 AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCACTTG ATGCTCGATG
     7741 AGTTTTTCTA ATGAGGACCT AAATGTAATC ACCTGGCTCA CCTTCGGGTG GGCCTTTCTG
     7801 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGATGCT
     7861 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
     7921 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
     7981 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
     8041 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
     8101 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
     8161 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
     8221 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC
     8281 TGAAGCCAGT TACCTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     8341 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     8401 AGAAGATCCT TTGATTTTCT ACCGAAGAAA GGCCCA
//