LOCUS       Exported                5550 bp ds-DNA     circular SYN 19-MAY-2025
DEFINITION  JG202.
ACCESSION   JG202
VERSION     .
KEYWORDS    pUHD10-3-human CDK11 p110B  D543-->N mut. cat.-FLAG.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 19, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(171..1031)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1032..1136)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1250..1520
                     /label=tetracycline response element
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    1531..1548
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=31.6"
                     /note="/other=misc_feature"
     promoter        1553..1591
                     /label=minimal CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=97.4"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1742..4069
                     /codon_start=1
                     
                     /label=CDK11A
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=88.9"
                     /note="/match_length=99.1"
                     /note="/other=CDS"
                     /translation="MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKR
                     DSLEEGELRDHCMEITIRNSPYRREDSMEDRGEEDDSLAIKPPQQMSWKEKVHHRKDEK
                     RKEKWKHARVKEREHERRKRHREEQDKARREWERQKRREMAREHSRRERGNDGVCLFRD
                     RLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMREDYSDK
                     VKASHWSRSPPRPPRERFELGDGRKPVKEEKMEERDLLSDLQDISDSERKTSSAESSSA
                     ESGSGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSE
                     DEERENENHLLVVPESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALLPIEL
                     KQELPKYLPALQGCRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEG
                     FPITSLREINTILKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLP
                     GEVKTLMIQLLRGVKHLHDNWILHRNLKTSNLLLSHAGILKVGDFGLAREYGSPLKAYT
                     PVVVTQWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGNSEIDQINKVFKEL
                     GTPSEKIWPGYSELPVVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRIS
                     AEDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKE
                     TGFHLTTTNQGASAAGPGFSLKF"
     misc_feature    3368..3370
                     /label= aa D543-->N mut. cat.
     misc_feature    4076..4099
                     /label=FLAG
     polyA_signal    4108..4242
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(4342..4572)
                     /codon_start=1
                     /label=str (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.7"
                     /note="/match_length=28.9"
                     /note="/other=CDS"
                     /translation="SSWILPVRQGDMPAMLKVARIPDEEAGYRLLTWWDGQGAARVFAS
                     AAGALLMERASGAGDLAQIAWSGQDDEACRII"
     promoter        4583..4611
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=28.2"
                     /note="/other=promoter"
     CDS             4612..4728
                     /codon_start=1
                     /label=CmR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=17.7"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQL"
     rep_origin      4962..5550
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     1201 aaaaataggc gtatcacgag gccctttcgt cttcactcga gtttaccact ccctatcagt
     1261 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     1321 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     1381 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     1441 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     1501 ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg
     1561 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     1621 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     1681 ccccgaattc gctagctcgc gaactcgagg atatcgtcga ggtcatcctg tgttaactca
     1741 aatgggtgat gaaaaggact cttggaaagt gaaaacttta gatgaaattc ttcaggaaaa
     1801 gaaacgaagg aaggaacaag aggagaaagc agagataaaa cgcttaaaaa attctgatga
     1861 ccgggattcc aagcgggatt cccttgagga gggggagctg agagatcact gcatggagat
     1921 cacaataagg aactccccgt atagaagaga agactcaatg gaagacagag gagaagaaga
     1981 tgattctttg gccatcaaac caccccagca aatgtcttgg aaagaaaaag ttcatcacag
     2041 aaaagatgaa aagaggaaag aaaaatggaa gcatgctaga gtgaaagaaa gagagcacga
     2101 acgtcggaaa cgacatcgag aagaacagga taaagctcgc cgggaatggg aaagacagaa
     2161 gagaagggaa atggcaaggg agcattccag gagagaaagg gggaatgatg gcgtgtgcct
     2221 cttcagggac cgcttggagc agttagaaag gaagcgggag cgggagcgca agatgcggga
     2281 gcagcagaag gagcagcggg agcagaagga gcgcgagcgg cgggcggagg agcggcgcaa
     2341 ggagcgggag gcccgcaggg aagtgtctgc acatcaccga acgatgagag aggactacag
     2401 cgacaaagtg aaagccagcc actggagtcg cagcccgcct cggccgccgc gggagcggtt
     2461 cgagttggga gacggccgga agccagtaaa agaagagaaa atggaagaaa gggacctgct
     2521 gtccgactta caggacatca gcgacagcga gaggaagacc agctcggccg agtcctcgtc
     2581 agcagaatca ggctcaggtt ctgaggaaga agaggaggag gaggaagagg aggaggagga
     2641 agggagcacc agtgaagaat cagaggagga agaggaagag gaggaggagg agaccggcag
     2701 caactctgag gaggcatcag agcagtctgc cgaagaagta agtgaggaag aaatgagtga
     2761 agatgaagaa cgagaaaatg aaaaccacct cttggttgtt ccagagtcac ggttcgaccg
     2821 agattccggg gagagtgaag aagcagagga agaagtgggt gagggaacgc cgcagagcag
     2881 cgccctgaca gagggcgact atgtgcccga ctcccctgcc ctgttgccca tcgagctcaa
     2941 gcaggagctg cccaagtacc tgccggccct gcagggctgc cggagcgtcg aggagttcca
     3001 gtgcctgaac aggatcgagg agggcaccta tggagtggtc tacagagcaa aagacaagaa
     3061 aacagatgaa attgtggctc taaagcggct gaagatggag aaggagaagg agggcttccc
     3121 gatcacgtcc ctgagggaga tcaacaccat cctcaaggcc cagcatccca acattgtcac
     3181 cgttagagag attgtggtgg gcagcaacat ggacaagatc tacatcgtga tgaactatgt
     3241 ggagcacgac ctcaagagcc tgatggagac catgaaacag cccttcctgc caggggaggt
     3301 gaagaccctg atgatccagc tgctgcgtgg ggtgaaacac ctgcacgaca actggatcct
     3361 gcaccgtaac ctcaagacgt ccaacctgct gctgagccac gccggcatcc tcaaggtggg
     3421 tgattttggg ctggcgcggg agtacggatc ccctctgaag gcctacaccc cggtcgtggt
     3481 gacccagtgg taccgcgccc cagagctgct gcttggtgcc aaggaatact ccacggccgt
     3541 ggacatgtgg tcagtgggct gcatcttcgg ggagctgctg actcagaagc ctctgttccc
     3601 cgggaattcg gaaatcgatc agatcaacaa agtgttcaag gagctgggga cccccagtga
     3661 gaaaatctgg cccggctaca gtgagctccc agtagtcaaa aagatgacct tcagcgagca
     3721 cccctacaac aacctccgca agcgcttcgg ggctctgctc tcagaccagg gcttcgacct
     3781 catgaacaag ttcctgacct acttccccgg gaggaggatc agcgctgagg acggcctcaa
     3841 gcatgagtat ttccgcgaga cccccctccc catcgacccc tccatgttcc ccacgtggcc
     3901 cgccaagagc gagcagcagc gtgtgaagcg gggcaccagc ccgaggcccc ctgagggagg
     3961 cctgggctac agccagctgg gtgacgacga cctgaaggag acgggcttcc accttaccac
     4021 cacgaaccag ggggcctctg ccgcgggccc cggcttcagc ctcaagttca agcttgacta
     4081 caaggacgac gatgacaagg tctagaggat ccagacatga taagatacat tgatgagttt
     4141 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     4201 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     4261 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     4321 tacaaatgtg gtatggctga ttatgatcct gcaagcctcg tcgtcctggc cggaccacgc
     4381 tatctgtgca aggtccccgg ccccggacgc gcgctccatg agcagagcgc ccgccgccga
     4441 ggcgaagact cgggcggcgc cctgcccgtc ccaccaggtc aacaggcggt aaccggcctc
     4501 ttcatcggga atgcgcgcga ccttcagcat cgccggcatg tccccctggc ggacgggaag
     4561 tatccagctc gaccaagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa
     4621 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     4681 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgca ttaatgaatc
     4741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5521 cagattacgc gcagaaaaaa aggatctcaa
//
