LOCUS       pCut_GFP                4054 bp ds-DNA     circular SYN 15-JAN-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   .
  TITLE     .
  JOURNAL   .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(39..60)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=95.5"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_RNA        complement(124..202)
                     /label=tracrRNA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
     promoter        complement(203..237)
                     /label=J23100 [MA]
                     /note="manually annotated"
                     /note="https://parts.igem.org/Promoters/Catalog/Anderson"
     promoter        complement(213..237)
                     /label=J23119(SpeI) promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.0"
                     /note="/match_length=71.4"
                     /note="/other=promoter"
     misc_feature    256..387
                     /label=crRNA leader
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     repeat_region   388..423
                     /label=DR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     misc_feature    424..453
                     /label=CS1 [MA]
                     /note="manually annotated"
                     /note="this study"
     repeat_region   454..489
                     /label=DR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     misc_feature    490..519
                     /label=CS2 [MA]
                     /note="manually annotated"
                     /note="this study"
     repeat_region   520..555
                     /label=DR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     misc_feature    556..585
                     /label=CS3 [MA]
                     /note="manually annotated"
                     /note="this study"
     repeat_region   586..621
                     /label=DR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     terminator      complement(688..782)
                     /label=lambda t0 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(811..1671)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1672..1735)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     terminator      complement(1762..1809)
                     /label=T7 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(1817..1834)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=14.3"
                     /note="/other=CDS"
     misc_RNA        complement(1862..1937)
                     /label=gRNA scaffold
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
     misc_feature    complement(1939..1944)
                     /label=BsmBI recognition site [MA]
                     /note="manually annotated"
                     /note="source: NEB"
     promoter        1954..1988
                     /label=J23100 [MA]
                     /note="manually annotated"
                     /note="https://parts.igem.org/Promoters/Catalog/Anderson"
     promoter        1954..1978
                     /label=J23119(SpeI) promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.0"
                     /note="/match_length=71.4"
                     /note="/other=promoter"
     CDS             2071..2784
                     /label=Folding Reporter GFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     terminator      2788..2836
                     /label=L3S3P22 [MA]
                     /note="manually annotated"
                     /note="https://doi.org/10.1038/nmeth.2515"
     misc_feature    2837..2858
                     /label=I-CreI recognition site [MA]
                     /note="manually annotated"
                     /note="https://doi.org/10.1038/s41598-018-28599-0"
     misc_feature    2859..2864
                     /label=BsmBI recognition site [MA]
                     /note="manually annotated"
                     /note="source: NEB"
     promoter        complement(2866..2919)
                     /label=PLtetO-1 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=73.0"
                     /note="/other=promoter"
     repeat_region   2993..3028
                     /label=DR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     CDS             complement(3029..3058)
                     /label=bla(M) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.8"
                     /note="/other=CDS"
     repeat_region   3059..3094
                     /label=DR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     rep_origin      3094..3125
                     /label=ColA ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.0"
                     /note="/other=rep_origin"
     repeat_region   3125..3160
                     /label=DR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     promoter        complement(3161..3277)
                     /label=rhaB promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.3"
                     /note="/other=promoter"
     rep_origin      complement(3294..3929)
                     /direction=LEFT
                     /label=ColA ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(join(4014..4054,1..24))
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.5"
                     /note="/other=CDS"
ORIGIN
        1 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
       61 attgcgttgc gcacaccgac tagcgaaaaa accccgccga agcggggttt tttgcgaaaa
      121 aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg
      181 ctatgctgtt ttgaatggtt ccgctagcac tgtacctagg actgagctag ccgtcaaaag
      241 cagcgtatat accattattt cttaataact aaaaatatgg tataatactc ttaataaatg
      301 cagtaataca ggggcttttc aagactgaag tctagctgag acaaatagtg cgattacgaa
      361 attttttaga caaaaatagt ctacgaggtt ttagagctat gctgttttga atggtcccaa
      421 aactatgcgg aattccagtc gcgttgatac gtggttttag agctatgctg ttttgaatgg
      481 tcccaaaact taacacaatt ttaagaccac cagcgccgcg ttttagagct atgctgtttt
      541 gaatggtccc aaaactctag ctacttcgaa aaatcattcc ctcgtgtttt agagctatgc
      601 tgttttgaat ggtcccaaaa cttcagcaca ctgagacttg ttgagttgaa ttcaggcaaa
      661 atgtgcgcgg aacccctatg tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc
      721 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
      781 tccaagcgag ctctaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
      841 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
      901 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
      961 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1021 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1081 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     1141 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1201 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1261 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1321 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1381 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     1441 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1501 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1561 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1621 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1681 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttgg
     1741 acgtctaaga aaccatacaa acaaaaaacc cctcaagacc cgtttagagg ccccaagggg
     1801 ttatgctagt tattgctcag cggtggcagc agcctaggat accggtaggc tgcataaaaa
     1861 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
     1921 atttctagct ctaaaactga gacggcgttg cgcttgacgg ctagctcagt cctaggtaca
     1981 gtgctagcta ctagagatta aagaggagaa actatatatg aaagcaattt tcgtactgaa
     2041 acatcttaat catgctaagg aggttttcta atgagcaaag gagaagaact tttcactgga
     2101 gttgtgccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     2161 ggagagggtg aaggtgatgc tacatacgga aaactcaccc ttaaatttat ttgcactact
     2221 ggaaaactac ctgttccatg gccaacactt gtcactactc tgacctatgg cgttcaatgc
     2281 ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc catgcccgaa
     2341 ggttatgtac aggaacgcac tatatctttc aaagatgacg ggaactacaa gacgcgtgct
     2401 gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg tattgatttt
     2461 aaagaagatg gaaacattct cggacacaaa ctagagtaca actataactc acacaatgta
     2521 tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat tcgccacaac
     2581 attgaagatg gttccgttca actagcagac cattatcaac aaaatactcc aattggcgat
     2641 ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgccct ttcgaaagat
     2701 cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca
     2761 catggcatgg atgagctcta caaatgacca attattgaag gccgctaacg cggccttttt
     2821 ttgtttctgg tctccccaaa acgtcgtgag acagtttgcg tctcagtgct cagtatctct
     2881 atcactgata gggatgtcaa tctctatcac tgatagggat tataaccaga ctcgaggacc
     2941 agactttaat taaaaaaaaa accccgccct gtcaggggcg gggttttttt ttgttttggg
     3001 accattcaaa acagcatagc tctaaaaccg agacccacgc tcaccggctc cagatttagt
     3061 tttgggacca ttcaaaacag catagctcta aaacagcttg gagcgaactg tctacacgga
     3121 acgggttttg ggaccattca aaacagcata gctctaaaac acgaccagtc taaaaagcgc
     3181 ctgaattcgc gaccttctcg ttactgacag gaaaatgggc cattggcaac cagggaaaga
     3241 tgaacgtgat gatgttcaca atttgctgaa ttgtggtagg catgctagcg cagaaacgtc
     3301 ctagaagatg ccaggaggat acttagcaga gagacaataa ggccggagcg aagccgtttt
     3361 tccataggct ccgcccccct gacgaacatc acgaaatctg acgctcaaat cagtggtggc
     3421 gaaacccgac aggactataa agataccagg cgtttccccc tgatggctcc ctcttgcgct
     3481 ctcctgttcc cgtcctgcgg cgtccgtgtt gtggtggagg ctttacccaa atcaccacgt
     3541 cccgttccgt gtagacagtt cgctccaagc tgggctgtgt gcaagaaccc cccgttcagc
     3601 ccgactgctg cgccttatcc ggtaactatc atcttgagtc caacccggaa agacacgaca
     3661 aaacgccact ggcagcagcc attggtaact gagaattagt ggatttagat atcgagagtc
     3721 ttgaagtggt ggcctaacag aggctacact gaaaggacag tatttggtat ctgcgctcca
     3781 ctaaagccag ttaccaggtt aagcagttcc ccaactgact taaccttcga tcaaaccgcc
     3841 tccccaggcg gttttttcgt ttacagagca ggagattacg acgatcgtaa aaggatctca
     3901 agaagatcct ttacggattc ccgacaccat cactctagat ttcagtgcaa tttatctctt
     3961 caaatttagc acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat
     4021 gccccgcgcc caccggaagg agctgactgg gttg
//
