LOCUS       VB211013-1375amc       11339 bp    DNA     circular SYN 20-MAY-2025
DEFINITION  
ACCESSION   VB211013-1375amc
VERSION     VB211013-1375amc
KEYWORDS    .
SOURCE      
  ORGANISM  .
            
FEATURES             Location/Qualifiers
     source          1..11339
     Promoter        1..229
                     /application_notes="Strong promoter; drives transcription
                     of viral RNA in packaging cells."
                     /description="Rous sarcoma virus enhancer/promoter"
                     /full_name="Rous sarcoma virus (RSV) enhancer/promoter"
                     /label="RSV  promoter"
                     /note="color: #05696d; direction: RIGHT"
                     /official_designation="RSV"
                     /uuid="3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /application_notes="Allows transcription of viral RNA and
                     its packaging into virus."
                     /description="Truncated HIV-1 5' long terminal repeat"
                     /full_name="HIV-1 truncated 5' LTR"
                     /label="5' LTR-ΔU3"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="Delta5' LTR"
                     /uuid="44156a3083604321be44066133e8ecb9"
     Miscellaneous   521..565
                     /application_notes="Allows packaging of viral RNA into
                     virus."
                     /description="HIV-1 packaging signal"
                     /full_name="HIV-1 psi packaging signal"
                     /label="Ψ"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="Psi"
                     /uuid="284899413ff943a99f8210a11ad8c54e"
     Miscellaneous   1075..1308
                     /application_notes="Rev protein binding site that allows
                     Rev-dependent nuclear export of viral RNA during viral
                     packaging."
                     /description="HIV-1 Rev response element"
                     /full_name="HIV-1 Rev response element"
                     /label="RRE"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="RRE"
                     /uuid="df07099e49674cf3aba3661b54e28bd7"
     Miscellaneous   1803..1920
                     /application_notes="Facilitates the nuclear import of HIV-1
                     cDNA through a central DNA flap."
                     /description="Central polypurine tract"
                     /full_name="Central polypurine tract"
                     /label="cPPT"
                     /note="color: #6d3ed8; direction: RIGHT"
                     /official_designation="cPPT"
                     /uuid="f7cb29985f9f41a7a5924d3462266f42"
     Promoter        1959..3137
                     /application_notes="Strong promoter."
                     /description="Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /full_name="Human eukaryotic translation elongation factor
                     1 alpha1 promoter"
                     /label="EF1A"
                     /note="color: #0375bb; direction: RIGHT"
                     /official_designation="EF1A"
                     /uuid="69c052aac71a4f49bab5b7ae1f46ace8"
     Miscellaneous   3162..3167
                     /application_notes="Facilitates translation initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /description="Kozak translation initiation sequence"
                     /full_name="Kozak"
                     /label="Kozak"
                     /note="color: #a949ca; direction: RIGHT"
                     /official_designation="Kozak"
                     /uuid="5f18b25a1234443494a618363f09f052"
     CDS             3168..5585
                     /application_notes=""
                     /label="{FUCCIMOX}"
                     /note="color: #68e66d; direction: RIGHT"
                     /official_designation="{FUCCIMOX}"
                     /uuid="fda1294cdd104553b96340e8035a5af9"
     Miscellaneous   5624..6221
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
                     /note="color: #946a00; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
     Promoter        6240..6750
                     /application_notes="Medium-strength promoter."
                     /description="Mouse phosphoglycerate kinase 1 promoter"
                     /full_name="Mouse phosphoglycerate kinase 1 promoter"
                     /label="mPGK promoter"
                     /note="color: #512bbd; direction: RIGHT"
                     /official_designation="mPGK promoter"
                     /uuid="bce93795b5fd4173b8b9abc832edad80"
     CDS             6763..7788
                     /application_notes="Allows cells to be resistant to
                     hygromycin B."
                     /description="Hygromycin resistance gene"
                     /full_name="Hygromycin resistance gene"
                     /label="Hygro"
                     /note="color: #d84e4e; direction: RIGHT"
                     /official_designation="Hygro"
                     /uuid="9135dd0aa64645f3aa3c381325794022"
     LTR             7859..8093
                     /application_notes="Allows packaging of viral RNA into
                     virus; self-inactivates the 5' LTR by a copying mechanism
                     during viral genome integration; contains polyadenylation
                     signal for transcription termination."
                     /description="Truncated HIV-1 3' long terminal repeat"
                     /full_name="HIV-1 truncated 3' LTR"
                     /label="3' LTR-ΔU3"
                     /note="color: #1f36a9; direction: RIGHT"
                     /official_designation="DeltaU3/3' LTR"
                     /uuid="c958755414c34372b909e20e81ebcef6"
     PolyA_signal    8166..8300
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Simian virus 40 early polyadenylation signal"
                     /full_name="SV40 early polyadenation signal"
                     /label="SV40 early pA"
                     /note="color: #db6901; direction: RIGHT"
                     /official_designation="SV40 early pA"
                     /uuid="2fcb13a62e704d8095b188d9625a6a94"
     CDS             9254..10114
                     /application_notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /label="AmpiciIIin"
                     /note="color: #800c0c; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="6a7994dc58024d80b36e4a85ad2e1d99"
     Rep_origin      10285..10873
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /note="color: #0c5d90; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gtgagcaagg
     3181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3301 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3361 tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     3421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3601 actactttag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     3661 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     3721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3781 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3841 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtacagc atgctgccta
     3901 gcctcggcgg ctaccctagc cattggaggc ccctggaacc ctctcctgcc agacctgccc
     3961 tgagagcccc tgcctccgcc acctccggca gcagaaagag agccagacct cctgccgccc
     4021 ctggcagaga tcaggccagg ccccctgccc gcagacggct ccggctgtcc gtggatgagg
     4081 tgtccagccc tagcaccccc gaggcccccg atatccctgc ctgtcccagc cctggccaga
     4141 aaatcaagaa gagcacacct gccgccggac agcctcctca cctgaccagc gcccaggacc
     4201 aggacaccat cgtcgacgag ggcaggggca gcctgctgac ctgcggcgac gtggaggaga
     4261 accccggccc cagatccatg gtagcaggtc atgcctctgg cagccccgca ttcgggaccg
     4321 cctctcattc gaattgcgaa catgaagaga tccacctcgc cggctcgatc cagccgcatg
     4381 gcgcgcttct ggtcgtcagc gaacatgatc atcgcgtcat ccaggccagc gccaacgccg
     4441 cggaatttct gaatctcgga agcgtactcg gcgttccgct cgccgagatc gacggcgatc
     4501 tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg gtcgcggtgc
     4561 gctgccggat cggcaatccc tctacggagt actgcggtct gatgcatcgg cctccggaag
     4621 gcgggctgat catcgaactc gaacgtgccg gcccgtcgat cgatctgtca ggcacgctgg
     4681 cgccggcgct ggagcggatc cgcacggcgg gttcactgcg cgcgctgtgc gatgacaccg
     4741 tgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgt ttcgatgagc
     4801 aaggccacgg cctggtattc tccgagtgcc atgtgcctgg gctcgaatcc tatttcggca
     4861 accgctatcc gtcgtcgact gtcccgcaga tggcgcggca gctgtacgtg cggcagcgcg
     4921 tccgcgtgct ggtcgacgtc acctatcagc cggtgccgct ggagccgcgg ctgtcgccgc
     4981 tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg ccgtgccatc
     5041 tgcagttcct gaaggacatg ggcgtgcgcg ccaccctggc ggtgtcgctg gtggtcggcg
     5101 gcaagctgtg gggcctggtt gtctgtcacc attatctgcc gcgcttcatc cgtttcgagc
     5161 tgcgggcgat ctgcaaacgg ctcgccgaaa ggatcgcgac gcggatcacc gcgcttgaga
     5221 gcggctaccc cagccactgg cggcctctgg aaatgaaccc cagcatgaag cagaagcagg
     5281 aagagatcaa agagaacatc aagaacagca gcgtgcccag gcggaccctg aagatgatcc
     5341 agcccagcgc cagcggatct ctggtgggcc gggagaatga gctgtctgcc ggcctgagca
     5401 agcggaagca ccggaacgac cacctgacca gcaccacctc tagcccaggc gtgatcgtgc
     5461 ccgagagcag cgagaacaag aacctgggcg gcgtgaccca agaaagcttc gacctgatga
     5521 tcaaagaaaa ccccagcagc cagtactgga aagaggtggc cgagaagcgg cggaaggccc
     5581 tgtaaaccca gctttcttgt acaaagtggt gataatcgaa ttccgataat caacctctgg
     5641 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5701 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5761 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5821 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5881 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5941 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6001 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6061 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6121 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6181 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcccg cggttcgaat
     6241 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     6301 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     6361 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     6421 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     6481 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     6541 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     6601 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     6661 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     6721 cctcttcctc atctccgggc ctttcgacct cacgtggcca ccatgaaaaa gcctgaactc
     6781 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     6841 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     6901 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     6961 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt tagcgagagc
     7021 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     7081 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     7141 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     7201 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     7261 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     7321 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     7381 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     7441 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     7501 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     7561 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     7621 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     7681 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     7741 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaataggg tacctttaag
     7801 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7861 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7921 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7981 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8041 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8101 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8161 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8221 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8281 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     8341 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8401 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8461 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     8521 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     8581 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     8641 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     8701 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8761 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8821 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8881 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8941 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9001 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9061 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     9121 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9181 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9241 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     9301 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     9361 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     9421 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     9481 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     9541 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9601 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9661 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9721 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9781 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9841 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9901 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9961 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    10021 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    10081 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    10141 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10201 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10621 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10681 agaaagcgcc acgcttcccg aagagagaaa ggcggacagg tatccggtaa gcggcagggt
    10741 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10801 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10861 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10921 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10981 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    11041 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11101 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    11161 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    11221 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
    11281 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctt
//
