LOCUS       Exported                8239 bp ds-DNA     circular SYN 02-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Monique Merchant
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 20, 2025 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7 fwd
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   708..942
                     /label=PB 3' TR
     promoter        1145..1536
                     /label=Second generation TRE promoter
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1501..1520
                     /label=PB-T fwd
     primer_bind     1561..1575
                     /label=OROV Mseg NheI fwd
     regulatory      1567..1576
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1573..1620
                     /codon_start=1
                     /label=OROV Gn Signal Sequence
                     /label=ORF1
                     /translation="MANLIIISMVLGVAYG"
     primer_bind     complement(1606..1633)
                     /label=PB2_CRIV.REV
     primer_bind     1609..1642
                     /label=CRIV2.FOR
     primer_bind     complement(1609..1620)
                     /label=PB backbone rev
     CDS             1621..2931
                     /codon_start=1
                     /label=CRIV Gc Spike H6
                     /translation="TEFETCIKKVEMTPECTGPFMEVGICNHQQKSLRYHEIANMLLGK
                     GTISKLDVDIIDALPDKIAGDLQVIQSSSDFHHQQMLEYVFLLRHCDYYSSFVSDNSYS
                     QILWQTAAKLNEINLCKHESSKKSCKCLKSNANCGESEDINTAAQAYYKNKESELGQDL
                     TTIMYILKKMIPGSGYSYLLNATKHNKLELFKAYMTNISSKYPNNKKLKDFVAITNAIL
                     ADLSALSHSSEESIPEYKQIDFSSLVGETTDMLEANFNQTISTDKIVCENPKVMICISP
                     RVSSGELYVCQKLGKYYIIDTGRFKLYKLVDADNLYCAADAQCRYEFRILTTGEMQATF
                     KDNCKASIHTNTPGYQNEAITHCKVLANGKCKYDNQTLIDVTLCANNRYYPEAKGRIFG
                     VDPEDLCFDKTCTKLQTPYNEELISMCVWEKHHHHHH"
     CDS             1621..2931
                     /codon_start=1
                     /label=CRIV Gc Spike H6
                     /translation="TEFETCIKKVEMTPECTGPFMEVGICNHQQKSLRYHEIANMLLGK
                     GTISKLDVDIIDALPDKIAGDLQVIQSSSDFHHQQMLEYVFLLRHCDYYSSFVSDNSYS
                     QILWQTAAKLNEINLCKHESSKKSCKCLKSNANCGESEDINTAAQAYYKNKESELGQDL
                     TTIMYILKKMIPGSGYSYLLNATKHNKLELFKAYMTNISSKYPNNKKLKDFVAITNAIL
                     ADLSALSHSSEESIPEYKQIDFSSLVGETTDMLEANFNQTISTDKIVCENPKVMICISP
                     RVSSGELYVCQKLGKYYIIDTGRFKLYKLVDADNLYCAADAQCRYEFRILTTGEMQATF
                     KDNCKASIHTNTPGYQNEAITHCKVLANGKCKYDNQTLIDVTLCANNRYYPEAKGRIFG
                     VDPEDLCFDKTCTKLQTPYNEELISMCVWEKHHHHHH"
     CDS             1621..2907
                     /codon_start=1
                     /label=Cristoli virus (CRIV) Gc spike
                     /label=ORF1
                     /translation="TEFETCIKKVEMTPECTGPFMEVGICNHQQKSLRYHEIANMLLGK
                     GTISKLDVDIIDALPDKIAGDLQVIQSSSDFHHQQMLEYVFLLRHCDYYSSFVSDNSYS
                     QILWQTAAKLNEINLCKHESSKKSCKCLKSNANCGESEDINTAAQAYYKNKESELGQDL
                     TTIMYILKKMIPGSGYSYLLNATKHNKLELFKAYMTNISSKYPNNKKLKDFVAITNAIL
                     ADLSALSHSSEESIPEYKQIDFSSLVGETTDMLEANFNQTISTDKIVCENPKVMICISP
                     RVSSGELYVCQKLGKYYIIDTGRFKLYKLVDADNLYCAADAQCRYEFRILTTGEMQATF
                     KDNCKASIHTNTPGYQNEAITHCKVLANGKCKYDNQTLIDVTLCANNRYYPEAKGRIFG
                     VDPEDLCFDKTCTKLQTPYNEELISMCVWE"
     primer_bind     complement(2884..2920)
                     /label=CRIV2.REV
     primer_bind     2896..2930
                     /label=PB2_CRIV.FOR
     CDS             2908..2928
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=LysHis6
                     /translation="KHHHHHH"
     CDS             2911..2928
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=His6
                     /translation="HHHHHH"
     primer_bind     2944..2968
                     /label=PB Gc backbone_fwd
     primer_bind     2949..2987
                     /label=PB backbone_fwd
     misc_feature    2962..3550
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    2962..3550
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3015..3035)
                     /label=WPRE-R
     CDS             complement(3433..3444)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3644..3661)
                     /label=BGH rev
     polyA_signal    3650..3874
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4158..4205
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      4299..4434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4438..5040
                     /label=Puromycin resistance marker
     polyA_signal    5274..5395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5591..5903
                     /label=PB 5' TR
     promoter        complement(6048..6066)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6087..6103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6111..6127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6135..6165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6180..6201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6489..7077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7248..8108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8109..8213)
                     /gene="bla"
                     /label=AmpR promoter
BASE COUNT         2071 a         2234 c         2006 g         1927 t
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 gctagcgcca ccatggccaa cctgatcatc atcagcatgg tgctgggcgt cgcctacggc
     1621 acagagtttg agacatgcat caagaaagtc gagatgacgc ccgagtgcac aggccccttt
     1681 atggaagtgg gcatctgcaa ccaccagcag aagtccctga gataccacga gatcgccaac
     1741 atgctgctcg gcaagggcac catcagcaag ctggacgtgg acatcatcga cgccctgcct
     1801 gataagattg ccggcgacct gcaagtgatc cagagcagca gcgatttcca tcatcagcag
     1861 atgctggaat acgtgttcct gctgcggcac tgcgactact acagcagctt cgtgtccgac
     1921 aacagctaca gccagatcct gtggcagaca gccgccaagc tgaacgagat caacctgtgc
     1981 aagcacgaga gcagcaagaa aagctgcaag tgcctgaagt ccaacgccaa ctgtggcgag
     2041 agcgaggaca tcaatactgc cgctcaggcc tactacaaga acaaagagag cgaactcggc
     2101 caggacctga ccaccatcat gtacatcctg aagaagatga tccccggcag cggctacagc
     2161 tacctgctga atgccaccaa gcacaacaag ctggaactgt tcaaggccta catgaccaac
     2221 atcagcagca agtaccccaa caacaagaaa ctgaaggact tcgtggccat caccaacgcc
     2281 attctggccg atctgtctgc cctgagccac agcagcgagg aatctatccc cgagtacaag
     2341 cagatcgact tcagcagcct cgtgggcgag acaaccgata tgctggaagc caacttcaac
     2401 cagaccatct ccaccgacaa gatcgtgtgc gagaacccca aagtgatgat ctgcatcagc
     2461 cccagagtgt ccagcggcga gctgtacgtg tgtcagaagc tgggcaagta ctatatcatc
     2521 gacaccggca ggttcaagct gtacaagctg gtggacgccg acaacctgta ctgtgccgct
     2581 gatgcccagt gcagatacga gttcagaatc ctgaccacag gcgagatgca ggccaccttc
     2641 aaggacaact gcaaggccag catccacacc aacacacccg gctaccagaa cgaggccatc
     2701 acacactgta aagtgctggc caacggcaag tgcaaatacg acaatcagac cctgatcgac
     2761 gtgaccctgt gcgccaacaa ccggtactac cctgaggcca agggcagaat ctttggcgtg
     2821 gaccctgagg acctgtgctt cgacaagacc tgcaccaagc tgcagacccc ttacaacgag
     2881 gaactgatct ctatgtgcgt gtgggagaag catcaccacc atcatcactg acttaagggt
     2941 accgaattcg atatcaagct taatcaacct ctggattaca aaatttgtga aagattgact
     3001 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3061 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3121 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3181 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3241 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3301 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     3361 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3421 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3481 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3541 gcctccccgc gcggccgcat agataactga tccagtgtgc tgggctacga tgcatgggcc
     3601 ccctaggtcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     3661 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     3721 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3781 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     3841 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     3901 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3961 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4021 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4081 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4141 gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga
     4201 acttccttgg ccaaaaagac cggtaccagg caggcagaag tatgcaaagc atgcatctca
     4261 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4321 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4381 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggctaccatg
     4441 ggtaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     4501 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     4561 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4621 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4681 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4741 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4801 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4861 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4921 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4981 gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga
     5041 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tggctccgac
     5101 cgaagccgac ccgggcggcc ccgccgaccc cgcacccgcc cccgaggccc accgactcta
     5161 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     5221 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     5281 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5341 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     5401 tcgagtgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5461 accgtcgacc tctagctgca ggaattcgat atcaagctct agccaacaag ctcgtcatcg
     5521 ctttgcagaa gagcagagag gatatgctca tcgtctaaag aactacccat tttattatat
     5581 attagtcacg atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa
     5641 catgaaataa caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat
     5701 catgcgtcat tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt
     5761 gacaagcacg cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac
     5821 ggattcgcgc tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg
     5881 cagactatct ttctagggtt aaaaaagatt tgcgctttac tcgacctaaa ctttaaacac
     5941 gtcatagaat cttcgtttga caaaaaccac attgtggcca agctgtgtga cgcgacgcgc
     6001 gctaaagaat ggcaaaccaa gtcgcgcgag gtacccagct tttgttccct ttagtgaggg
     6061 ttaattccga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6121 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6181 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6241 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6301 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6901 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     6961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8221 ccccgaaaag tgccacctg
//
