LOCUS       Exported                9228 bp ds-DNA     circular SYN 25-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(719..823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(849..1304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(916..941)
                     /label=3'SacI_C-SV40polyA
     primer_bind     1443..1462
                     /label=GMD/pPthc_REVseq
     primer_bind     1446..1462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1579..1604
                     /label=GMD/blunt_PiggyBac_FWD/REV
     misc_feature    1592..1604
                     /label=PB Terminal repeat
     misc_feature    1639..1654
                     /label=IR (inverted repeat) truncated?
     primer_bind     2063..2093
                     /label=5'NruI_MluI_PGK_promoter
     promoter        2073..2572
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     2505..2525
                     /label=GMD/PGKprom_FWDseq2
     primer_bind     2584..2614
                     /label=5'HindIII_kozac_Puro
     CDS             2594..3193
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3176..3199)
                     /label=3'NotI_Puro_ver3
     primer_bind     3206..3223
                     /label=GMD-TH/pcDNA3.1_vector_GA_FWD
     primer_bind     complement(3220..3237)
                     /label=pcDNA3.1/BGH rev
     polyA_signal    3226..3450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3404..3423
                     /label=GMD-SC/bGHpolyA_fwdSEQ
     primer_bind     complement(3443..3470)
                     /label=3'AsiSI__bGH poly(A)
     promoter        3489..3864
                     /label=L30 prom
                     /note="L30 prom"
     primer_bind     3734..3753
                     /label=ES_SH_009/L30prom_forSEQ
     primer_bind     3734..3752
                     /label=prAH331
     primer_bind     complement(3846..3868)
                     /label=3'AvrII_L30prom
     primer_bind     3860..3895
                     /label=VF462_PB_l30_AD_GibsonF1
     primer_bind     3877..3895
                     /label=VF417_PB_AD_GibsonF1
     promoter        3877..3881
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             3896..4012
                     /codon_start=1
                     /label=VP16_H1_39
                     /translation="PTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSP"
     CDS             4025..4915
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     primer_bind     4913..4931
                     /label=VF511_synTF_ERD_F
     primer_bind     complement(4916..4950)
                     /label=VF418_PB_AD_GibsonR1
     CDS             4925..5809
                     /codon_start=1
                     /locus_tag="Estrogen Binding Domain"
                     /label=Estrogen Binding Domain
                     /translation="SAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPI
                     LYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLE
                     ILMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEE
                     FVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQR
                     LAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEE
                     TDQSHLATAGSTSS"
     primer_bind     5795..5817
                     /label=VF419_PB_ZFs_GibsonF2
     primer_bind     complement(5796..5821)
                     /label=VF512_synTF_ERD_R
     CDS             5816..6337
                     /codon_start=1
                     /locus_tag="ZF1-3"
                     /label=ZF1-3
                     /translation="SRPGERPFQCRICMRNFSEEANLRRHTRTHTGEKPFQCRICMRNF
                     SDHSSLKRHLRTHTGSQKPFQCRICMRNFSQSANLLRHTRTHTGEKPFQCRICMRNFSD
                     PSSLKRHLRTHTGSQKPFQCRICMRNFSQQTNLTRHTRTHTGEKPFQCRICMRNFSDAT
                     QLVRHLRTHLR"
     primer_bind     complement(6331..6409)
                     /label=VF420_PGK_PB_ZFs_GibsonR2
     primer_bind     complement(6331..6372)
                     /label=VF421_PB_IRES_ZFs_GibsonR2
     primer_bind     complement(6331..6370)
                     /label=VF463_PB_ZFs_GibsonR1
     CDS             6347..6349
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="A"
     CDS             6350..6391
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     6350..6371
                     /label=VF464_PB_V5_GibsonF2
     CDS             6350..6351
                     /codon_start=1
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="wings apart-like protein homolog isoform 2"
                     /label=Wapl
                     /note="isoform 2 is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS79296.1"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
                     /protein_id="NP_001288259.1"
                     /translation=""
     CDS             6350..6351
                     /codon_start=1
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="wings apart-like protein homolog isoform X1"
                     /label=Wapl
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
                     /protein_id="XP_006518887.1"
                     /translation=""
     mRNA            6350..6351
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     2"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     mRNA            6350..6351
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     1"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     mRNA            6350..6351
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     X1"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 3 mRNAs, 179 ESTs, 583 long SRA 
                     reads, and 100% coverage of the annotated genomic feature 
                     by RNAseq alignments, including 18 samples with support for
                     all annotated introns"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     gene            6350..6351
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /label=Wapl
                     /note="WAPL cohesin release factor; Derived by automated 
                     computational analysis using gene prediction method: 
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     primer_bind     complement(6367..6394)
                     
     primer_bind     complement(6368..6391)
                     /label=2928/V5_noSTOP_GArev
     primer_bind     complement(6373..6401)
                     /label=3'SalI_stop_V5
     mRNA            6391..6395
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /product="cyclin dependent kinase 9"
                     /label=CDK9 mRNA
                     /db_xref="GeneID:1025"
                     /db_xref="HGNC:HGNC:1780"
                     /db_xref="MIM:603251"
     exon            6391..6395
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /number=7
                     /inference="alignment:same species:2.1.0"
     misc_feature    6391
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /note="propagated from UniProtKB/Swiss-Prot (P50750.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     primer_bind     complement(6476..6495)
                     /label=wPRE rev seq
     polyA_signal    6516..6637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6881..6902)
                     /label=VF465_PB_V5_GibsonR2
     misc_feature    complement(7400..7418)
                     /label=IR (inverted repeat)
     primer_bind     complement(7422..7447)
                     /label=GMD/blunt_PiggyBac_FWD/REV
     misc_feature    complement(7422..7434)
                     /label=PB Terminal repeat
     primer_bind     complement(7925..7941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7938..7960)
                     /label=GMD/M13_REV_Yick
     protein_bind    7949..7965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7973..8003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     7977..7993
                     /label=GWc-R_attR1jct
     protein_bind    8018..8039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     8107..8150
                     /label=5'NruI_MluI_PGK_promoter
     primer_bind     complement(8151..8170)
                     /label=pQE SEQ1
     primer_bind     complement(8290..8309)
                     /label=GMD/pEGFP minusCMV forSEQ
     rep_origin      complement(8327..8915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8942..8961
                     /label=pQE SEQ2
     primer_bind     8952..8971
                     /label=LZ6 REV SEQ
     CDS             complement(join(9086..9228,1..718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1021 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1081 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1141 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1201 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1261 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     1321 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     1381 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1441 acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga
     1501 ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct
     1561 cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta
     1621 cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     1681 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     1741 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa
     1801 tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg
     1861 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     1921 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     1981 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     2041 cctgggggga tacggggaaa aggcgaacgc gtgggtaggg gaggcgcttt tcccaaggca
     2101 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     2161 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     2221 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     2281 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     2341 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     2401 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     2461 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     2521 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     2581 cccaagcttc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2641 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2701 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     2821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     2881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     2941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3181 gcccggtgcc tgagcggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     3241 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3301 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3361 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3421 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaacgcgat atgtacgggc
     3481 cagatatacg cgtcgcagaa caaacgccca gaaccgaagg ccggagccca gacgccgcag
     3541 cagcccagcc tgcgttgtaa cccggtcccc atagcaacca actaccgcag actactctcg
     3601 caagcgctgt tctgcttgtg acccggaccg gcttctcttc cgcttccggt cccacaatcc
     3661 tctgtcggcc tagaagagct ttgcattgtg ggagctcctt cctttctcgc tccccggcca
     3721 tcttggcggc tggtgttggt gagtgagctc tgcggggtaa acgattaggc ggctcgggga
     3781 gctccgctag ctggtgtttg acgctctgga ttataatcct ctcttctgtc ctctgtgtat
     3841 gctaggttgg gggttgtccc gcacctagag ctagcgccca gcttaagcca ccatgcccac
     3901 cgacgtgagc ctgggcgacg agctgcacct ggacggcgag gacgtggcca tggcccacgc
     3961 cgacgccctg gacgacttcg acctggacat gctgggcgac ggcgacagcc ccggatccgg
     4021 cagcatggcc gagatcggaa ccggcttccc tttcgaccct cactacgtgg aggtgctggg
     4081 agaacggatg cactacgtgg acgtgggacc tagagacgga acaccagtgc tgttcctgca
     4141 cggaaacccc acaagcagct acgtctggcg gaacatcatc ccccacgtgg cccctacaca
     4201 cagatgtatc gccccagacc tgatcggcat gggaaagagc gacaagcccg acctgggcta
     4261 cttcttcgac gaccacgtgc ggttcatgga cgctttcatc gaggccctgg gactggaaga
     4321 ggtggtgctg gtcatccacg attggggaag cgctctgggc ttccattggg ccaagaggaa
     4381 cccagagagg gtcaagggca tcgccttcat ggagttcatc cggcctatcc ccacttggga
     4441 cgagtggcca gaattcgcca gggagacctt ccaggctttc agaaccaccg acgtgggcag
     4501 gaagctgatc atcgaccaga acgtgttcat cgagggaacc ctgcctatgg gagtcgtgag
     4561 acctctgacc gaggtggaga tggaccacta cagggagccc ttcctgaacc cagtggacag
     4621 agagcctctg tggagattcc ccaacgagct gcctatcgcc ggagaaccag ccaatatcgt
     4681 ggccctggtg gaggagtaca tggattggct gcaccagagc cccgtgccta agctgctgtt
     4741 ttggggcaca ccaggagtgc tgattcctcc agcagaagcc gctagactgg ccaaaagcct
     4801 gcctaattgc aaggccgtgg acatcggccc aggactgaat ctgctgcagg aggacaaccc
     4861 cgacctgatt ggaagcgaga ttgcccggtg gctcagtacc ctggagatca gcggcagcgc
     4921 tggctctgct ggagacatga gagctgccaa cctttggcca agcccgctca tgatcaaacg
     4981 ctctaagaag aacagcctgg ccttgtccct gacggccgac cagatggtca gtgccttgtt
     5041 ggatgctgag ccccccatac tctattccga gtatgatcct accagaccct tcagtgaagc
     5101 ttcgatgatg ggcttactga ccaacctggc agacagggag ctggttcaca tgatcaactg
     5161 ggcgaagagg gtgccaggct ttgtggattt gaccctccat gatcaggtcc accttctaga
     5221 atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg cgctccatgg agcacccagt
     5281 gaagctactg tttgctccta acttgctctt ggacaggaac cagggaaaat gtgtagaggg
     5341 catggtggag atcttcgaca tgctgctggc tacatcatct cggttccgca tgatgaatct
     5401 gcagggagag gagtttgtgt gcctcaaatc tattattttg cttaattctg gagtgtacac
     5461 atttctgtcc agcaccctga agtctctgga agagaaggac catatccacc gagtcctgga
     5521 caagatcaca gacactttga tccacctgat ggccaaggca ggcctgaccc tgcagcagca
     5581 gcaccagcgg ctggcccagc tcctcctcat cctctcccac atcaggcaca tgagtaacaa
     5641 aggcatggag catctgtaca gcatgaagtg caagaacgtg gtgcccctct atgacctgct
     5701 gctggagatg ctggacgccc accgcctaca tgcgcccact agccgtggag gggcatccgt
     5761 ggaggagacg gaccaaagcc acttggccac tgcgggctct acttcatcgg gtacctctag
     5821 accaggcgaa cgaccgtttc aatgccggat atgtatgagg aacttctccg aggaggcaaa
     5881 cttgaggcgc cacacccgaa cacatacagg agaaaagcca ttccaatgtc gaatttgtat
     5941 gcgcaatttt tcagatcact caagcctcaa gcgacacctc cgcacacata ctggttcaca
     6001 gaagcccttt cagtgcagga tttgtatgcg caactttagc caatcagcga accttttgcg
     6061 gcacactaga acgcatacag gtgagaagcc tttccagtgt cgcatctgta tgcggaactt
     6121 cagcgacccc agttcattga agaggcattt gcgaactcac accggttctc aaaaaccttt
     6181 tcagtgccga atttgtatgc gcaacttcag ccaacaaaca aatttgacga gacacacgcg
     6241 cacgcacacc ggggaaaaac cgtttcaatg tcgaatctgc atgcgcaatt ttagcgatgc
     6301 gacacaactt gttaggcatc tgcgcacaca cttgcgggga agcggagctg gtaagcctat
     6361 ccctaaccct ctcctcggtc tcgattctac ctgagtcgac aatcaacctc tggattacaa
     6421 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6481 cgctgcttta atgcctttgt atcatgcgtt aactaaactt gtttattgca gcttataatg
     6541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat
     6661 gatgtcaatt agtctatcag aagctcatct ggtctccctt ccgggggaca agacatccct
     6721 gtttaatatt taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa
     6781 ccaggttgca gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc
     6841 aggtttagcc ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc
     6901 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     6961 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     7021 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     7081 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctgatat ctataacaag
     7141 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     7201 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     7261 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     7321 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     7381 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc
     7441 tagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg
     7501 cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca
     7561 tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt
     7621 accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag
     7681 gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg
     7741 aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag
     7801 attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg
     7861 atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat
     7921 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7981 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8041 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8101 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8161 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8221 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8281 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8341 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8401 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8461 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8521 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8581 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8641 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8701 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8761 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8821 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8881 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8941 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9001 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9061 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9121 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9181 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgag
//
