LOCUS       Exported                9231 bp ds-DNA     circular SYN 30-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(719..823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(849..1304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(916..941)
                     /label=3'SacI_C-SV40polyA
     primer_bind     1443..1462
                     /label=GMD/pPthc_REVseq
     primer_bind     1446..1462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1579..1604
                     /label=GMD/blunt_PiggyBac_FWD/REV
     misc_feature    1592..1604
                     /label=PB Terminal repeat
     misc_feature    1639..1654
                     /label=IR (inverted repeat) truncated?
     primer_bind     2063..2093
                     /label=5'NruI_MluI_PGK_promoter
     promoter        2073..2572
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     2505..2525
                     /label=GMD/PGKprom_FWDseq2
     primer_bind     2584..2614
                     /label=5'HindIII_kozac_Puro
     CDS             2594..3193
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3176..3199)
                     /label=3'NotI_Puro_ver3
     primer_bind     3206..3223
                     /label=GMD-TH/pcDNA3.1_vector_GA_FWD
     primer_bind     complement(3220..3237)
                     /label=pcDNA3.1/BGH rev
     polyA_signal    3226..3450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3404..3423
                     /label=GMD-SC/bGHpolyA_fwdSEQ
     primer_bind     complement(3443..3470)
                     /label=3'AsiSI__bGH poly(A)
     promoter        3489..3864
                     /label=L30 prom
                     /note="L30 prom"
     primer_bind     3734..3753
                     /label=ES_SH_009/L30prom_forSEQ
     primer_bind     3734..3752
                     /label=prAH331
     primer_bind     complement(3846..3868)
                     /label=3'AvrII_L30prom
     primer_bind     3860..3895
                     /label=VF462_PB_l30_AD_GibsonF1
     primer_bind     3877..3895
                     /label=VF417_PB_AD_GibsonF1
     promoter        3877..3881
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     misc_feature    3887..3895
                     /label=Kozak+ATG
     CDS             3896..4015
                     /codon_start=1
                     /label=CITED2_killmotif1
                     /translation="DTDAIDEEVAMSAVIEMGLDRIKELPELWLGQNEFDFMTD"
     CDS             4028..4918
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     primer_bind     complement(4919..4953)
                     /label=VF418_PB_AD_GibsonR1
     CDS             4928..5812
                     /codon_start=1
                     /locus_tag="Estrogen Binding Domain"
                     /label=Estrogen Binding Domain
                     /translation="SAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPI
                     LYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLE
                     ILMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEE
                     FVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQR
                     LAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEE
                     TDQSHLATAGSTSS"
     primer_bind     5798..5820
                     /label=VF419_PB_ZFs_GibsonF2
     CDS             5819..6340
                     /codon_start=1
                     /locus_tag="ZF1-3"
                     /label=ZF1-3
                     /translation="SRPGERPFQCRICMRNFSEEANLRRHTRTHTGEKPFQCRICMRNF
                     SDHSSLKRHLRTHTGSQKPFQCRICMRNFSQSANLLRHTRTHTGEKPFQCRICMRNFSD
                     PSSLKRHLRTHTGSQKPFQCRICMRNFSQQTNLTRHTRTHTGEKPFQCRICMRNFSDAT
                     QLVRHLRTHLR"
     primer_bind     complement(6334..6412)
                     /label=VF420_PGK_PB_ZFs_GibsonR2
     primer_bind     complement(6334..6375)
                     /label=VF421_PB_IRES_ZFs_GibsonR2
     primer_bind     complement(6334..6373)
                     /label=VF463_PB_ZFs_GibsonR1
     CDS             6350..6352
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="A"
     CDS             6353..6394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     6353..6374
                     /label=VF464_PB_V5_GibsonF2
     CDS             6353..6354
                     /codon_start=1
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="wings apart-like protein homolog isoform 2"
                     /label=Wapl
                     /note="isoform 2 is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS79296.1"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
                     /protein_id="NP_001288259.1"
                     /translation=""
     CDS             6353..6354
                     /codon_start=1
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="wings apart-like protein homolog isoform X1"
                     /label=Wapl
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
                     /protein_id="XP_006518887.1"
                     /translation=""
     mRNA            6353..6354
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     2"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     mRNA            6353..6354
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     1"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     mRNA            6353..6354
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     X1"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 3 mRNAs, 179 ESTs, 583 long SRA 
                     reads, and 100% coverage of the annotated genomic feature 
                     by RNAseq alignments, including 18 samples with support for
                     all annotated introns"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     gene            6353..6354
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /label=Wapl
                     /note="WAPL cohesin release factor; Derived by automated 
                     computational analysis using gene prediction method: 
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     primer_bind     complement(6370..6397)
                     
     primer_bind     complement(6371..6394)
                     /label=2928/V5_noSTOP_GArev
     primer_bind     complement(6376..6404)
                     /label=3'SalI_stop_V5
     mRNA            6394..6398
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /product="cyclin dependent kinase 9"
                     /label=CDK9 mRNA
                     /db_xref="GeneID:1025"
                     /db_xref="HGNC:HGNC:1780"
                     /db_xref="MIM:603251"
     exon            6394..6398
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /number=7
                     /inference="alignment:same species:2.1.0"
     misc_feature    6394
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /note="propagated from UniProtKB/Swiss-Prot (P50750.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     primer_bind     complement(6479..6498)
                     /label=wPRE rev seq
     polyA_signal    6519..6640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6884..6905)
                     /label=VF465_PB_V5_GibsonR2
     misc_feature    complement(7403..7421)
                     /label=IR (inverted repeat)
     primer_bind     complement(7425..7450)
                     /label=GMD/blunt_PiggyBac_FWD/REV
     misc_feature    complement(7425..7437)
                     /label=PB Terminal repeat
     primer_bind     complement(7928..7944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7941..7963)
                     /label=GMD/M13_REV_Yick
     protein_bind    7952..7968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7976..8006)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     7980..7996
                     /label=GWc-R_attR1jct
     protein_bind    8021..8042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     8110..8153
                     /label=5'NruI_MluI_PGK_promoter
     primer_bind     complement(8154..8173)
                     /label=pQE SEQ1
     primer_bind     complement(8293..8312)
                     /label=GMD/pEGFP minusCMV forSEQ
     rep_origin      complement(8330..8918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8945..8964
                     /label=pQE SEQ2
     primer_bind     8955..8974
                     /label=LZ6 REV SEQ
     CDS             complement(join(9089..9231,1..718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1021 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1081 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1141 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1201 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1261 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     1321 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     1381 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1441 acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga
     1501 ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct
     1561 cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta
     1621 cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     1681 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     1741 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa
     1801 tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg
     1861 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     1921 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     1981 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     2041 cctgggggga tacggggaaa aggcgaacgc gtgggtaggg gaggcgcttt tcccaaggca
     2101 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     2161 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     2221 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     2281 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     2341 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     2401 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     2461 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     2521 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     2581 cccaagcttc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2641 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2701 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     2821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     2881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     2941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3181 gcccggtgcc tgagcggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     3241 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3301 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3361 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3421 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaacgcgat atgtacgggc
     3481 cagatatacg cgtcgcagaa caaacgccca gaaccgaagg ccggagccca gacgccgcag
     3541 cagcccagcc tgcgttgtaa cccggtcccc atagcaacca actaccgcag actactctcg
     3601 caagcgctgt tctgcttgtg acccggaccg gcttctcttc cgcttccggt cccacaatcc
     3661 tctgtcggcc tagaagagct ttgcattgtg ggagctcctt cctttctcgc tccccggcca
     3721 tcttggcggc tggtgttggt gagtgagctc tgcggggtaa acgattaggc ggctcgggga
     3781 gctccgctag ctggtgtttg acgctctgga ttataatcct ctcttctgtc ctctgtgtat
     3841 gctaggttgg gggttgtccc gcacctagag ctagcgccca gcttaagcca ccatggacac
     3901 cgacgccatt gacgaagagg tggccatgag cgccgtgatt gagatgggac tggacaggat
     3961 caaggagctg ccagagctgt ggctgggaca gaacgagttc gacttcatga ccgacggatc
     4021 cggcagcatg gccgagatcg gaaccggctt ccctttcgac cctcactacg tggaggtgct
     4081 gggagaacgg atgcactacg tggacgtggg acctagagac ggaacaccag tgctgttcct
     4141 gcacggaaac cccacaagca gctacgtctg gcggaacatc atcccccacg tggcccctac
     4201 acacagatgt atcgccccag acctgatcgg catgggaaag agcgacaagc ccgacctggg
     4261 ctacttcttc gacgaccacg tgcggttcat ggacgctttc atcgaggccc tgggactgga
     4321 agaggtggtg ctggtcatcc acgattgggg aagcgctctg ggcttccatt gggccaagag
     4381 gaacccagag agggtcaagg gcatcgcctt catggagttc atccggccta tccccacttg
     4441 ggacgagtgg ccagaattcg ccagggagac cttccaggct ttcagaacca ccgacgtggg
     4501 caggaagctg atcatcgacc agaacgtgtt catcgaggga accctgccta tgggagtcgt
     4561 gagacctctg accgaggtgg agatggacca ctacagggag cccttcctga acccagtgga
     4621 cagagagcct ctgtggagat tccccaacga gctgcctatc gccggagaac cagccaatat
     4681 cgtggccctg gtggaggagt acatggattg gctgcaccag agccccgtgc ctaagctgct
     4741 gttttggggc acaccaggag tgctgattcc tccagcagaa gccgctagac tggccaaaag
     4801 cctgcctaat tgcaaggccg tggacatcgg cccaggactg aatctgctgc aggaggacaa
     4861 ccccgacctg attggaagcg agattgcccg gtggctcagt accctggaga tcagcggcag
     4921 cgctggctct gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa
     4981 acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt
     5041 gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga
     5101 agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa
     5161 ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct
     5221 agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc
     5281 agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga
     5341 gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa
     5401 tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta
     5461 cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct
     5521 ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca
     5581 gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa
     5641 caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct
     5701 gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc
     5761 cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat cgggtacctc
     5821 tagaccaggc gaacgaccgt ttcaatgccg gatatgtatg aggaacttct ccgaggaggc
     5881 aaacttgagg cgccacaccc gaacacatac aggagaaaag ccattccaat gtcgaatttg
     5941 tatgcgcaat ttttcagatc actcaagcct caagcgacac ctccgcacac atactggttc
     6001 acagaagccc tttcagtgca ggatttgtat gcgcaacttt agccaatcag cgaacctttt
     6061 gcggcacact agaacgcata caggtgagaa gcctttccag tgtcgcatct gtatgcggaa
     6121 cttcagcgac cccagttcat tgaagaggca tttgcgaact cacaccggtt ctcaaaaacc
     6181 ttttcagtgc cgaatttgta tgcgcaactt cagccaacaa acaaatttga cgagacacac
     6241 gcgcacgcac accggggaaa aaccgtttca atgtcgaatc tgcatgcgca attttagcga
     6301 tgcgacacaa cttgttaggc atctgcgcac acacttgcgg ggaagcggag ctggtaagcc
     6361 tatccctaac cctctcctcg gtctcgattc tacctgagtc gacaatcaac ctctggatta
     6421 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6481 atacgctgct ttaatgcctt tgtatcatgc gttaactaaa cttgtttatt gcagcttata
     6541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6601 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg aattgactca
     6661 aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg acaagacatc
     6721 cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc ttgctctggt
     6781 caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga aacagtggca
     6841 gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg tatggctttt
     6901 tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa
     6961 gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat
     7021 gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg
     7081 ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga tatctataac
     7141 aagaaaatat atatataata agttatcacg taagtagaac atgaaataac aatataatta
     7201 tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg
     7261 cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag
     7321 ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag
     7381 agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta
     7441 atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc
     7501 tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg
     7561 gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg aaaacgcacg
     7621 tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga actgttcgtt
     7681 caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg gatggtcagc
     7741 ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg
     7801 cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg gtatcctggc
     7861 tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg cgcttggcgt
     7921 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7981 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8041 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8101 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8161 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8221 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8281 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8341 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8401 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8461 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8521 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8581 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8641 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8701 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8761 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8821 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8881 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8941 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9001 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9061 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9121 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9181 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga g
//
