LOCUS       Exported                9249 bp ds-DNA     circular SYN 15-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(719..823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(849..1304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(916..941)
                     /label=3'SacI_C-SV40polyA
     primer_bind     1443..1462
                     /label=GMD/pPthc_REVseq
     primer_bind     1446..1462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1579..1604
                     /label=GMD/blunt_PiggyBac_FWD/REV
     misc_feature    1592..1604
                     /label=PB Terminal repeat
     misc_feature    1639..1654
                     /label=IR (inverted repeat) truncated?
     primer_bind     2063..2093
                     /label=5'NruI_MluI_PGK_promoter
     promoter        2073..2572
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     2505..2525
                     /label=GMD/PGKprom_FWDseq2
     primer_bind     2584..2614
                     /label=5'HindIII_kozac_Puro
     CDS             2594..3193
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3176..3199)
                     /label=3'NotI_Puro_ver3
     primer_bind     3206..3223
                     /label=GMD-TH/pcDNA3.1_vector_GA_FWD
     primer_bind     complement(3220..3237)
                     /label=pcDNA3.1/BGH rev
     polyA_signal    3226..3450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3404..3423
                     /label=GMD-SC/bGHpolyA_fwdSEQ
     primer_bind     complement(3443..3470)
                     /label=3'AsiSI__bGH poly(A)
     promoter        3489..3864
                     /label=L30 prom
                     /note="L30 prom"
     primer_bind     3734..3753
                     /label=ES_SH_009/L30prom_forSEQ
     primer_bind     3734..3752
                     /label=prAH331
     primer_bind     complement(3846..3868)
                     /label=3'AvrII_L30prom
     primer_bind     3860..3895
                     /label=VF462_PB_l30_AD_GibsonF1
     primer_bind     3877..3895
                     /label=VF417_PB_AD_GibsonF1
     promoter        3877..3881
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     misc_feature    3887..3895
                     /label=Kozak+ATG
     CDS             3896..4033
                     /codon_start=1
                     /label=HIF1a_AD2_46_RK>D
                     /translation="DLLGQSMDESGLPQLTSYDCEVNAPIQGSDNLLQGEELLDALDQV
                     N"
     variation       3896..3898
                     /label=R>D
     variation       3983..3985
                     /label=R>D
     variation       4013..4015
                     /label=R>D
     CDS             4046..4936
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     primer_bind     complement(4937..4971)
                     /label=VF418_PB_AD_GibsonR1
     CDS             4946..5830
                     /codon_start=1
                     /locus_tag="Estrogen Binding Domain"
                     /label=Estrogen Binding Domain
                     /translation="SAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPI
                     LYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLE
                     ILMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEE
                     FVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQR
                     LAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEE
                     TDQSHLATAGSTSS"
     primer_bind     5816..5838
                     /label=VF419_PB_ZFs_GibsonF2
     CDS             5837..6358
                     /codon_start=1
                     /locus_tag="ZF1-3"
                     /label=ZF1-3
                     /translation="SRPGERPFQCRICMRNFSEEANLRRHTRTHTGEKPFQCRICMRNF
                     SDHSSLKRHLRTHTGSQKPFQCRICMRNFSQSANLLRHTRTHTGEKPFQCRICMRNFSD
                     PSSLKRHLRTHTGSQKPFQCRICMRNFSQQTNLTRHTRTHTGEKPFQCRICMRNFSDAT
                     QLVRHLRTHLR"
     primer_bind     complement(6352..6430)
                     /label=VF420_PGK_PB_ZFs_GibsonR2
     primer_bind     complement(6352..6393)
                     /label=VF421_PB_IRES_ZFs_GibsonR2
     primer_bind     complement(6352..6391)
                     /label=VF463_PB_ZFs_GibsonR1
     CDS             6368..6370
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="A"
     CDS             6371..6412
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     6371..6392
                     /label=VF464_PB_V5_GibsonF2
     CDS             6371..6372
                     /codon_start=1
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="wings apart-like protein homolog isoform 2"
                     /label=Wapl
                     /note="isoform 2 is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS79296.1"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
                     /protein_id="NP_001288259.1"
                     /translation=""
     CDS             6371..6372
                     /codon_start=1
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="wings apart-like protein homolog isoform X1"
                     /label=Wapl
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
                     /protein_id="XP_006518887.1"
                     /translation=""
     mRNA            6371..6372
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     2"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     mRNA            6371..6372
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     1"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     mRNA            6371..6372
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     X1"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 3 mRNAs, 179 ESTs, 583 long SRA 
                     reads, and 100% coverage of the annotated genomic feature 
                     by RNAseq alignments, including 18 samples with support for
                     all annotated introns"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     gene            6371..6372
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /label=Wapl
                     /note="WAPL cohesin release factor; Derived by automated 
                     computational analysis using gene prediction method: 
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     primer_bind     complement(6388..6415)
                     
     primer_bind     complement(6389..6412)
                     /label=2928/V5_noSTOP_GArev
     primer_bind     complement(6394..6422)
                     /label=3'SalI_stop_V5
     mRNA            6412..6416
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /product="cyclin dependent kinase 9"
                     /label=CDK9 mRNA
                     /db_xref="GeneID:1025"
                     /db_xref="HGNC:HGNC:1780"
                     /db_xref="MIM:603251"
     exon            6412..6416
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /number=7
                     /inference="alignment:same species:2.1.0"
     misc_feature    6412
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /note="propagated from UniProtKB/Swiss-Prot (P50750.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     primer_bind     complement(6497..6516)
                     /label=wPRE rev seq
     polyA_signal    6537..6658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6902..6923)
                     /label=VF465_PB_V5_GibsonR2
     misc_feature    complement(7421..7439)
                     /label=IR (inverted repeat)
     primer_bind     complement(7443..7468)
                     /label=GMD/blunt_PiggyBac_FWD/REV
     misc_feature    complement(7443..7455)
                     /label=PB Terminal repeat
     primer_bind     complement(7946..7962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7959..7981)
                     /label=GMD/M13_REV_Yick
     protein_bind    7970..7986
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7994..8024)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     7998..8014
                     /label=GWc-R_attR1jct
     protein_bind    8039..8060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     8128..8171
                     /label=5'NruI_MluI_PGK_promoter
     primer_bind     complement(8172..8191)
                     /label=pQE SEQ1
     primer_bind     complement(8311..8330)
                     /label=GMD/pEGFP minusCMV forSEQ
     rep_origin      complement(8348..8936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8963..8982
                     /label=pQE SEQ2
     primer_bind     8973..8992
                     /label=LZ6 REV SEQ
     CDS             complement(join(9107..9249,1..718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1021 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1081 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1141 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1201 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1261 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     1321 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     1381 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1441 acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga
     1501 ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct
     1561 cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta
     1621 cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     1681 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     1741 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa
     1801 tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg
     1861 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     1921 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     1981 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     2041 cctgggggga tacggggaaa aggcgaacgc gtgggtaggg gaggcgcttt tcccaaggca
     2101 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     2161 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     2221 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     2281 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     2341 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     2401 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     2461 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     2521 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     2581 cccaagcttc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2641 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2701 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     2821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     2881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     2941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3181 gcccggtgcc tgagcggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     3241 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3301 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3361 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3421 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaacgcgat atgtacgggc
     3481 cagatatacg cgtcgcagaa caaacgccca gaaccgaagg ccggagccca gacgccgcag
     3541 cagcccagcc tgcgttgtaa cccggtcccc atagcaacca actaccgcag actactctcg
     3601 caagcgctgt tctgcttgtg acccggaccg gcttctcttc cgcttccggt cccacaatcc
     3661 tctgtcggcc tagaagagct ttgcattgtg ggagctcctt cctttctcgc tccccggcca
     3721 tcttggcggc tggtgttggt gagtgagctc tgcggggtaa acgattaggc ggctcgggga
     3781 gctccgctag ctggtgtttg acgctctgga ttataatcct ctcttctgtc ctctgtgtat
     3841 gctaggttgg gggttgtccc gcacctagag ctagcgccca gcttaagcca ccatggacct
     3901 gctgggccag agcatggacg agagcggcct gccccagctg accagctacg actgcgaggt
     3961 gaacgccccc atccagggca gcgacaacct gctgcagggc gaggagctgc tggacgccct
     4021 ggaccaggtg aacggatccg gcagcatggc cgagatcgga accggcttcc ctttcgaccc
     4081 tcactacgtg gaggtgctgg gagaacggat gcactacgtg gacgtgggac ctagagacgg
     4141 aacaccagtg ctgttcctgc acggaaaccc cacaagcagc tacgtctggc ggaacatcat
     4201 cccccacgtg gcccctacac acagatgtat cgccccagac ctgatcggca tgggaaagag
     4261 cgacaagccc gacctgggct acttcttcga cgaccacgtg cggttcatgg acgctttcat
     4321 cgaggccctg ggactggaag aggtggtgct ggtcatccac gattggggaa gcgctctggg
     4381 cttccattgg gccaagagga acccagagag ggtcaagggc atcgccttca tggagttcat
     4441 ccggcctatc cccacttggg acgagtggcc agaattcgcc agggagacct tccaggcttt
     4501 cagaaccacc gacgtgggca ggaagctgat catcgaccag aacgtgttca tcgagggaac
     4561 cctgcctatg ggagtcgtga gacctctgac cgaggtggag atggaccact acagggagcc
     4621 cttcctgaac ccagtggaca gagagcctct gtggagattc cccaacgagc tgcctatcgc
     4681 cggagaacca gccaatatcg tggccctggt ggaggagtac atggattggc tgcaccagag
     4741 ccccgtgcct aagctgctgt tttggggcac accaggagtg ctgattcctc cagcagaagc
     4801 cgctagactg gccaaaagcc tgcctaattg caaggccgtg gacatcggcc caggactgaa
     4861 tctgctgcag gaggacaacc ccgacctgat tggaagcgag attgcccggt ggctcagtac
     4921 cctggagatc agcggcagcg ctggctctgc tggagacatg agagctgcca acctttggcc
     4981 aagcccgctc atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga
     5041 ccagatggtc agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc
     5101 taccagaccc ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga
     5161 gctggttcac atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca
     5221 tgatcaggtc caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg
     5281 gcgctccatg gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa
     5341 ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc
     5401 tcggttccgc atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt
     5461 gcttaattct ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga
     5521 ccatatccac cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc
     5581 aggcctgacc ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca
     5641 catcaggcac atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt
     5701 ggtgcccctc tatgacctgc tgctggagat gctggacgcc caccgcctac atgcgcccac
     5761 tagccgtgga ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc
     5821 tacttcatcg ggtacctcta gaccaggcga acgaccgttt caatgccgga tatgtatgag
     5881 gaacttctcc gaggaggcaa acttgaggcg ccacacccga acacatacag gagaaaagcc
     5941 attccaatgt cgaatttgta tgcgcaattt ttcagatcac tcaagcctca agcgacacct
     6001 ccgcacacat actggttcac agaagccctt tcagtgcagg atttgtatgc gcaactttag
     6061 ccaatcagcg aaccttttgc ggcacactag aacgcataca ggtgagaagc ctttccagtg
     6121 tcgcatctgt atgcggaact tcagcgaccc cagttcattg aagaggcatt tgcgaactca
     6181 caccggttct caaaaacctt ttcagtgccg aatttgtatg cgcaacttca gccaacaaac
     6241 aaatttgacg agacacacgc gcacgcacac cggggaaaaa ccgtttcaat gtcgaatctg
     6301 catgcgcaat tttagcgatg cgacacaact tgttaggcat ctgcgcacac acttgcgggg
     6361 aagcggagct ggtaagccta tccctaaccc tctcctcggt ctcgattcta cctgagtcga
     6421 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6481 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcgt taactaaact
     6541 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6661 atgtctggaa ttgactcaaa tgatgtcaat tagtctatca gaagctcatc tggtctccct
     6721 tccgggggac aagacatccc tgtttaatat ttaaacagca gtgttcccaa actgggttct
     6781 tatatccctt gctctggtca accaggttgc agggtttcct gtcctcacag gaacgaagtc
     6841 cctaaagaaa cagtggcagc caggtttagc cccggaattg actggattcc ttttttaggg
     6901 cccattggta tggctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     6961 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     7021 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     7081 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct
     7141 gtccctgata tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat
     7201 gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat
     7261 gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac
     7321 aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga
     7381 ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag
     7441 actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg
     7501 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     7561 tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac
     7621 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     7681 aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     7741 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg
     7801 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     7861 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     7921 ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7981 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8041 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8101 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8161 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8221 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8281 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8341 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8401 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8461 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8521 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8581 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8641 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8701 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8761 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8821 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8881 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8941 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9001 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9061 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9121 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9181 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9241 taccgcgag
//
