LOCUS       Exported                9963 bp ds-DNA     circular SYN 05-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(192..201)
                     /label=VF140_hActb_gRNAE2F
     primer_bind     complement(592..601)
                     /label=mOct4_qPCR_E1F
     promoter        complement(719..823)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(753..783)
                     /label=VF102_gRNA_puroR_GibR2
     primer_bind     755..786
                     /label=VF101_gRNA_puroR_GibF1
     rep_origin      complement(849..1303)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(916..941)
                     /label=attB2_C-SV40polyA_3'
     primer_bind     complement(916..941)
                     /label=5'SpeI_C-SV40polyA_op
     primer_bind     complement(916..941)
                     /label=3'SacI_C-SV40polyA
     primer_bind     complement(986..1007)
                     /label=GD3371_F1ori-FWDseq_(addgene)
     primer_bind     complement(1107..1116)
                     /label=GMD-NW/mAtoh1_OUTfwd2
     primer_bind     complement(1332..1354)
                     /label=GMD-CD/LacZ_sgRNA_top
     primer_bind     1426..1440
                     /label=M13 Forward (-40)
     primer_bind     1440..1461
                     /label=GMD/M13_FWD_Gina
     primer_bind     1441..1460
                     /label=GMD/pPthc_REVseq
     primer_bind     1444..1460
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1461..1471)
                     /label=IRES_GArev
     primer_bind     complement(1533..1542)
                     /label=5'SalI_1XF-hP2A (1)
     primer_bind     1543..1564
                     /label=GMD/5'SpeI_PiggyBac_FWD
     primer_bind     1577..1602
                     /label=GMD/blunt_PiggyBac_FWD/REV
     repeat_region   1590..1652
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2035..2044)
                     /label=JE19/eSpCas9s.seq4
     primer_bind     2069..2103
                     /label=GMD-LD/(PB AsiSI)-L30prom_GAfwd
     primer_bind     2081..2103
                     /label=GMD/5'AsiSI_L30prom
     misc_feature    2086..2462
                     /label=L30 prom
                     /note="L30 prom"
     primer_bind     2086..2103
                     /label=GD4095_(pX-C1)-L30_GAfwd
     primer_bind     2086..2095
                     /label=GMD-ASH/5'AsiSI_AscI_spacer_L30prom
     primer_bind     2092..2112
                     /label=GMD-CC/5'SpeI_L30prom
     primer_bind     2092..2112
                     /label=GMD/5'AseI_L30p
     primer_bind     2138..2148
                     /label=GMD-SC/5'HindIII_EWS_156_FIXED
     primer_bind     2138..2148
                     /label=GMD-SC/5'BsrGI_EWS_156
     primer_bind     2138..2148
                     /label=GMD-SC/5'HindIII_EWS_156
     primer_bind     2215..2224
                     /label=GD3848_TP53_gRNA#3N_sense
     primer_bind     2291..2302
                     /label=GD3385_SP1delA_OUTrev
     primer_bind     complement(2314..2324)
                     /label=5'NotI_1XF-mTAF9B
     primer_bind     complement(2314..2324)
                     /label=5'NotI_mTAF9B
     primer_bind     complement(2314..2324)
                     /label=5'SpeI_mTAF9b
     primer_bind     complement(2314..2324)
                     /label=5'XbaI_2_mTAF9b
     primer_bind     complement(2314..2324)
                     /label=5'XbaI_mTAF9b
     primer_bind     2331..2350
                     /label=ES_SH_009/L30prom_forSEQ
     primer_bind     complement(2376..2390)
                     /label=GMD/pFC_-B_PL_top
     primer_bind     complement(2443..2465)
                     /label=GMD/3'AgeI_L30prom
     primer_bind     complement(2443..2465)
                     /label=GMD-CC/L30prom_GA_vector_rev
     primer_bind     complement(2443..2465)
                     /label=GMD/3'AvrII_L30p
     primer_bind     complement(2447..2464)
                     /label=GMD-LD/(PB)-L30prom_GArev
     primer_bind     complement(2448..2469)
                     /label=GMD-NW/3'BamHI(-NotI-XbaI-AgeI)_L30prom
     primer_bind     complement(2448..2462)
                     /label=GMD-JK/L30prom_GArev_vector
     primer_bind     2461..2481
                     /label=L30_kozac_GAfwd
     primer_bind     2467..2494
                     /label=GD4282_(pcDNA3.1-KpnI)-kozac-V5_GAfwd
     primer_bind     2472..2505
                     /label=GD3700_(EF1a)-V5_GAfwd
     primer_bind     2472..2505
                     /label=GD3775_(EF1a)-V5_GAfwd
     primer_bind     2473..2555
                     /label=GMD-NW/5'NheI_ALFA_Halo
     primer_bind     2473..2549
                     /label=GD-zd/5'NheI_1XF_(Halo)
     primer_bind     2473..2505
                     /label=GMD-NW/5'NheI_kozac_V5_1
     primer_bind     2473..2494
                     /label=GD3593_5'SpeI_kozac_V5
     primer_bind     2473..2484
                     /label=GMD-NW/5'NheI_kozac_ATG-Gly
     primer_bind     complement(2473..2483)
                     /label=GD3837_ITR2_MCS_1_anti
     primer_bind     complement(2474..2483)
                     /label=GMD/3'BamHI_LifeAct_bot
     primer_bind     complement(2475..2526)
                     /label=GMD/3'BamHI_V5
     gene            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /label=ARNT
                     /note="aryl hydrocarbon receptor nuclear translocator; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq,Gnomon."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant 4"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant 3"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant 6"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant 1"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant 9"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant 8"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant 5"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant 7"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X16"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 138 ESTs, 88 long SRA reads, 2 
                     Proteins, and 99% coverage of the annotated genomic feature
                     by RNAseq alignments, including 76 samples with support for
                     all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X10"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 116 ESTs, 99 long SRA reads, 1 
                     Protein, and 99% coverage of the annotated genomic feature 
                     by RNAseq alignments, including 76 samples with support for
                     all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X15"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 142 ESTs, 90 long SRA reads, 1 
                     Protein, and 100% coverage of the annotated genomic feature
                     by RNAseq alignments, including 129 samples with support 
                     for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X14"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 150 ESTs, 87 long SRA reads, 2 
                     Proteins, and 100% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 90 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X9"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 1 mRNA, 158 ESTs, 101 long SRA 
                     reads, 3 Proteins, and 100% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 113 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X13"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 157 ESTs, 81 long SRA reads, 4 
                     Proteins, and 100% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 129 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X12"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 157 ESTs, 92 long SRA reads, 4 
                     Proteins, and 100% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 135 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X7"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 147 ESTs, 100 long SRA reads, 4 
                     Proteins, and 100% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 164 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X8"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 83 ESTs, 97 long SRA reads, 2 
                     Proteins, and 100% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 49 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X6"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 128 ESTs, 83 long SRA reads, 1 
                     Protein, and 100% coverage of the annotated genomic feature
                     by RNAseq alignments, including 66 samples with support for
                     all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X4"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 136 ESTs, 91 long SRA reads, 3 
                     Proteins, and 100% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 82 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X5"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 133 ESTs, 89 long SRA reads, 4 
                     Proteins, and 100% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 69 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X2"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 1 mRNA, 134 ESTs, 97 long SRA 
                     reads, 3 Proteins, and 100% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 63 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X1"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 75 ESTs, 57 long SRA reads, 4 
                     Proteins, and 100% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 83 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     mRNA            2478..2482
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator, 
                     transcript variant X3"
                     /label=ARNT mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 114 ESTs, 108 long SRA reads, 3 
                     Proteins, and 100% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 85 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
     CDS             2478..2482
                     /codon_start=1
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator 
                     isoform X15"
                     /label=ARNT
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
                     /protein_id="XP_016856785.1"
                     /translation="M"
     CDS             2478..2482
                     /codon_start=1
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator 
                     isoform 4"
                     /label=ARNT
                     /note="isoform 4 is encoded by transcript variant 4; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
                     /protein_id="NP_001184254.1"
                     /translation="M"
     CDS             2478..2482
                     /codon_start=1
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator 
                     isoform 3"
                     /label=ARNT
                     /note="isoform 3 is encoded by transcript variant 3; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS971.1"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
                     /protein_id="NP_848514.1"
                     /translation="M"
     CDS             2478..2482
                     /codon_start=1
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator 
                     isoform X14"
                     /label=ARNT
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
                     /protein_id="XP_016856784.1"
                     /translation="M"
     CDS             2478..2482
                     /codon_start=1
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator 
                     isoform X9"
                     /label=ARNT
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
                     /protein_id="XP_005245210.1"
                     /translation="M"
     CDS             2478..2482
                     /codon_start=1
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator 
                     isoform X13"
                     /label=ARNT
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
                     /protein_id="XP_016856783.1"
                     /translation="M"
     CDS             2478..2482
                     /codon_start=1
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator 
                     isoform X12"
                     /label=ARNT
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
                     /protein_id="XP_005245214.1"
                     /translation="M"
     CDS             2478..2482
                     /codon_start=1
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator 
                     isoform 6"
                     /label=ARNT
                     /note="isoform 6 is encoded by transcript variant 6; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS65642.1"
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
                     /protein_id="NP_001272965.1"
                     /translation="M"
     CDS             2478..2482
                     /codon_start=1
                     /gene="ARNT"
                     /gene_synonym="bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; 
                     HIF1B; HIF1BETA; TANGO"
                     /product="aryl hydrocarbon receptor nuclear translocator 
                     isoform X7"
                     /label=ARNT
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:405"
                     /db_xref="HGNC:HGNC:700"
                     /db_xref="MIM:126110"
                     /protein_id="XP_005245208.1"
                     /translation="M"
     CDS             2479..2481
                     /codon_start=1
                     /label=ATG
                     /translation="M"
     primer_bind     2482..2555
                     /label=GMD-YC/V5-Halo_GAfwd (74-mer):
     CDS             2482..2523
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(2482..2510)
                     /label=GD3438_(V5)-Halo_GArev
     primer_bind     2482..2505
                     /label=GD3412_V5_GAfwd
     primer_bind     complement(2482..2501)
                     /label=GD4350_hCDK9_N_LHArev_GA_V5
     primer_bind     2482..2500
                     /label=GMD-ASH/Halo-S(TCC)-V5_GAfwd
     primer_bind     complement(2499..2523)
                     /label=GMD-CD/V5_GArev
     primer_bind     complement(2500..2523)
                     /label=GMD-JK/3'AgeI_stop_V5
     primer_bind     complement(2500..2523)
                     /label=GMD-ASH/V5_noSTOP_GArev
     primer_bind     2500..2522
                     /label=GD3964_(V5)-NPM1_C_RHA_GAfwd
     primer_bind     complement(2501..2534)
                     /label=GMD-AH/3'NotI_V5(recode)
     primer_bind     complement(2503..2534)
                     /label=GD3594_3'NotI_stop_V5
     primer_bind     complement(2503..2527)
                     /label=GMD-NW/3'BglII_STOP_V5
     primer_bind     complement(2505..2527)
                     /label=GD3717_(IRES)-stop_V5_GArev
     primer_bind     complement(2506..2527)
                     /label=GD4107_(wPRE)-stop-V5_GArev
     primer_bind     complement(2509..2529)
                     /label=GMD-CD/GG(link)-V5_GArev
     primer_bind     complement(2509..2523)
                     /label=GMD-ASH/MUT_Stag2_gRNA#2_bot
     primer_bind     complement(2512..2523)
                     /label=GD4138_MUT_RXRA_gRNA#4C_anti
     primer_bind     2522..2543
                     /label=GMD-CD/(mAID)_Halo_GAfwd
     primer_bind     2523..2554
                     /label=5'XbaI_TEV_Halo
     primer_bind     complement(2523..2534)
                     /label=GMD-CD/hTAF4_RHAfwd_GA_TEV
     primer_bind     2523..2533
                     /label=GMD-CD/hTAF4_RHAfwd_GA_TEV
     primer_bind     2527..2543
                     /label=GMD-TH/5'BamHI(NotI)_GDGAGLIN_Halo
     CDS             2533..3420
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     primer_bind     2533..2559
                     /label=GMD/5'XhoI_Halo
     primer_bind     2533..2555
                     /label=GMD/5'PspOMI_Halo
     primer_bind     2533..2555
                     /label=HALO_GAfwd
     primer_bind     2533..2554
                     /label=GD3769_(hs vector)-Halo_GAfwd
     primer_bind     2533..2554
                     /label=GMD/5'HindIII_Halo
     primer_bind     2533..2550
                     /label=GMD/5'XbaI_AscI_link-Halo
     primer_bind     2533..2550
                     /label=GMD-AH/5'AgeI_GDGAGLIN_Halo
     primer_bind     2533..2550
                     /label=GD4363_(PB XbaI)-kozac_Halo_GAfwd
     primer_bind     2533..2550
                     /label=GMD-TG/(UAST)-Halo_GAfwd
     primer_bind     2533..2549
                     /label=GMD-SC/AgeI_kozac_Halo_GAfwd
     primer_bind     2533..2549
                     /label=GMD-NW/5' (kozac)_quasi Halo GAfwd
     primer_bind     2533..2549
                     /label=GMD-CD/add Gly_S-Halo_fwd
     primer_bind     2533..2548
                     /label=GD-zd/5'NheI_HALO_C1
     primer_bind     2533..2546
                     /label=GMD-Rio/5'NheI_GDGAGLIN_Halo
     primer_bind     2533..2546
                     /label=GMD-ASH/Ctcf Halo gRNA#3N_top
     primer_bind     2533..2543
                     /label=GD4365_5'AscI_kozac_Halo
     primer_bind     2534..2563
                     /label=GMD/5'PacI_HaloTag
     primer_bind     complement(2534..2555)
                     /label=VF346_FoxHT_genotypeR1
     primer_bind     complement(2540..2560)
                     /label=VF392_Foxa1TEVscreen-2R
     primer_bind     complement(2547..2565)
                     /label=HaloTag_genotype_R2
     primer_bind     complement(2548..2568)
                     /label=VF390_Foxa1TEVscreen-1R
     primer_bind     complement(2557..2576)
                     /label=GMD-ASH/Ctcf Halo gRNA#1N_top
     primer_bind     complement(2565..2588)
                     /label=GMD/CD439/Halo antisense (rev)
     primer_bind     2565..2588
                     /label=GMD-ASH/Halo sense (fwd)
     primer_bind     complement(2567..2587)
                     /label=HaloTag_genotype_R3
     primer_bind     complement(2575..2592)
                     /label=GMD/HT7 R
     primer_bind     2632..2652
                     /label=VF339_HT-int_F
     primer_bind     complement(2664..2683)
                     /label=VF416_Foxa1HT_genotypeout_R3
     primer_bind     2777..2796
                     /label=GMD/Halo_FWD
     primer_bind     complement(2839..2856)
                     /label=CT526_HALOrev
     primer_bind     complement(2853..2862)
                     /label=GMD-WD/5'BglII_BspEI_TEV
     primer_bind     complement(2853..2862)
                     /label=GMD-CD/TEV_fwd_GA
     primer_bind     complement(2853..2862)
                     /label=GMD/5'Acc65I_TEV
     primer_bind     complement(2853..2862)
                     /label=GMD/5'AgeI_TEV
     primer_bind     2894..2903
                     /label=VF264_hGapdh_LAMP4_LF
     primer_bind     3000..3009
                     /label=3'SalI_cFLAG_pET
     primer_bind     3177..3194
                     /label=SG_Halo_Internal_fwd1
     primer_bind     3184..3203
                     /label=HaloTag_genotype_F2
     primer_bind     complement(3226..3245)
                     /label=GMD/Halo_REV
     primer_bind     3230..3247
                     /label=SG_Halo_Internal_fwd
     primer_bind     3296..3315
                     /label=HaloTag_genotype_F1
     primer_bind     3300..3318
                     /label=GMD/HALOseqF
     primer_bind     complement(3348..3359)
                     /label=GMD-CD/3'AgeI_hSUPT7L
     primer_bind     3348..3358
                     /label=GMD-Robert/hSUPT7L_gRNA#5C_top
     primer_bind     3374..3393
                     /label=GMD-ASH/Ctcf Halo gRNA#4C_top
     primer_bind     3394..3411
                     /label=3138/5'XhoI_Halo(end)
     primer_bind     complement(3396..3417)
                     /label=3'BglII_stop_Halo
     primer_bind     complement(3397..3420)
                     /label=GMD/3'BglII_V5recode_Halo
     primer_bind     complement(3397..3417)
                     /label=GMD-TH/Halo_insert_GA_REV
     primer_bind     complement(3400..3420)
                     /label=GMD-CC/5'NotI_stop_Halo
     primer_bind     complement(3401..3447)
                     /label=GMD/3'BamHI_NotI_link_noSTOP_Halo
     primer_bind     complement(3401..3435)
                     /label=GMD-NW/3' quasi Halo_(S-GDGAGLIN-GS) GArev
     primer_bind     complement(3401..3420)
                     /label=GMD/3'AscI_HaloTag
     primer_bind     complement(3401..3420)
                     /label=3'BsiWI_noSTOP_1XF_Halo
     primer_bind     complement(3402..3453)
                     /label=GMD-YC/Halo-GDGAGLIN_vector_GArev
     primer_bind     complement(3402..3452)
                     /label=GMD-TH/3'XbaI_Halo-GDGAGLIN
     primer_bind     complement(3402..3420)
                     /label=VF384_Foxa1G275XmglnkHalo-R
     primer_bind     complement(3402..3420)
                     /label=VF370_Foxa1HAmglnkHalo-R
     primer_bind     complement(3402..3420)
                     /label=GD3438_(V5)-Halo_GArev
     primer_bind     complement(3403..3420)
                     /label=GMD-CD/Halo-TEV_GA_rev
     primer_bind     complement(3404..3420)
                     /label=GMD/3'MluI_stop_Halo
     primer_bind     3405..3420
                     /label=GMD-ASH/Halo-S(TCC)-V5_GAfwd
     primer_bind     complement(3405..3420)
                     /label=GMD-CD/(mAID)-link-Halo_GArev
     primer_bind     complement(3408..3426)
                     /label=VF340_HT-int_R
     primer_bind     complement(3409..3420)
                     /label=GMD/3'BamHI_3XF-Halo
     primer_bind     complement(3411..3420)
                     /label=GMD-NW/ALFA_XbaI_anti
     primer_bind     3420..3432
                     /label=GD3735_hHEXIM1_MUT_gRNA#2C_GAfwd
     primer_bind     3421..3437
                     /label=GMD-CD/S-GDGAGLIN_fwd_GA
     primer_bind     3422..3439
                     /label=VF383_Foxa1G275XmglnkHalo-F
     primer_bind     complement(3423..3447)
                     /label=GMD/3'BsiWI_GDGAGLIN_bot
     primer_bind     3423..3441
                     /label=GMD-NW/5'NotI(BamHI)_GDGAGLIN
     primer_bind     3423..3439
                     /label=VF369_Foxa1HAmglnkHalo-F
     primer_bind     complement(3423..3439)
                     /label=GD4175_(mglk)-noSTOP_hRXRA_GArev
     primer_bind     3423..3439
                     /label=GMD-YC/5'BamHI_GDGAGlLIN
     primer_bind     3423..3437
                     /label=GMD-TH/GDGAGLIN_insert_GA_FWD
     primer_bind     3423..3434
                     /label=GD4287_(BamHI GS)-mglk_GAfwd
     CDS             3424..3447
                     /codon_start=1
                     /label=GDGAGLIN linker
                     /translation="GDGAGLIN"
     primer_bind     3424..3441
                     /label=GMD-YC/GDGAGLIN_Gafwd
     primer_bind     3424..3441
                     /label=(3XF)-GDGAGLIN_GAFWD
     primer_bind     3427..3447
                     /label=3157/DGAGLIN_fwd
     primer_bind     complement(3427..3447)
                     /label=GMD-JK/DGAGLIN_REV
     primer_bind     complement(3432..3454)
                     /label=GMD-YC/GDGAGLIN-S_PL_Garev
     primer_bind     complement(3432..3452)
                     /label=GMD-CD/mglk-GS_TAF4
     primer_bind     complement(3432..3450)
                     /label=GMD-CD/mglk_GArev
     primer_bind     complement(3432..3450)
                     /label=GDGAGLIN-GSGS_GArev
     primer_bind     complement(3432..3449)
                     /label=GDGAGLIN-GS_(BamHI)_GArev
     primer_bind     complement(3433..3464)
                     /label=GMD-NW/3'NotI(XbaI)_GDGAGLIN
     primer_bind     complement(3433..3452)
                     /label=GDGAGLING-S_rev_GA
     primer_bind     complement(3433..3452)
                     /label=3124/3'EcoRI_GDGAGLIN
     primer_bind     complement(3433..3452)
                     /label=GMD-NW/3'BamHI_STOP_c-myc NLS
     primer_bind     3436..3476
                     /label=GMD-YC/HIF1a NT_RHAfwd_GA_link
     primer_bind     3436..3469
                     /label=(MGLK)-HIF1A_GAFWD_V2
     primer_bind     3436..3452
                     /label=(mglk)-HIF1a_364_GAfwd
     primer_bind     3438..3469
                     /label=(mglk)-HIF1a_GAfwd
     primer_bind     complement(3441..3452)
                     /label=GMD-YC/3'EcoRI_EX_MCS
     primer_bind     3448..3465
                     /label=GMD-YC/(3XF)-hHIF1a_GAfwd
     gene            3454..5934
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /label=HIF1A
                     /note="hypoxia inducible factor 1 subunit alpha"
                     /db_xref="GeneID:3091"
                     /db_xref="HGNC:HGNC:4910"
                     /db_xref="MIM:603348"
     CDS             3454..5934
                     /codon_start=1
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /product="hypoxia-inducible factor 1-alpha isoform 1"
                     /label=HIF1A
                     /note="isoform 1 is encoded by transcript variant 1; ARNT 
                     interacting protein; hypoxia inducible factor 1, alpha 
                     subunit (basic helix-loop-helix transcription factor); 
                     member of PAS superfamily 1; hypoxia-inducible factor 
                     1-alpha; hypoxia-inducible factor 1 alpha isoform I.3; 
                     member of PAS protein 1; PAS domain-containing protein 8; 
                     hypoxia inducible factor 1 alpha subunit; 
                     basic-helix-loop-helix-PAS protein MOP1; class E basic 
                     helix-loop-helix protein 78; hypoxia-inducible 
                     factor1alpha"
                     /db_xref="CCDS:CCDS9753.1"
                     /db_xref="GeneID:3091"
                     /db_xref="HGNC:HGNC:4910"
                     /db_xref="MIM:603348"
                     /protein_id="NP_001521.1"
                     /translation="MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLP
                     LPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLT
                     DDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNT
                     QRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVL
                     ICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHA
                     LDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNY
                     VVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTL
                     LAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAET
                     PKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPS
                     EYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRS
                     FDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDI
                     KILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVA
                     LSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWK
                     RVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPELTSYDCEVEAPIEGSRNL
                     LQGEELLRALDQVN"
     misc_feature    3454..4656
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: Interaction with TSGA10. {ECO:0000250}"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            3454..3488
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     primer_bind     3454..3469
                     /label=5'BspEI_hHIF1a
     primer_bind     3454..3469
                     /label=GMD/5'FseI_mHif1a_1
     primer_bind     3454..3464
                     /label=GMD-YC/HIF1a_NT_gRNA#2_sense
     exon            3489..3679
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3514..3543
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: DNA-binding. {ECO:0000250|UniProtKB:Q61221}"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     primer_bind     3538..3575
                     /label=(18)-HIF1a_31_GAfwd
     exon            3680..3825
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     exon            3826..3910
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     exon            3911..4023
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     primer_bind     3930..3943
                     /label=GD3650_hCDK9_gPCR_OUT_rev1
     primer_bind     complement(3941..3983)
                     /label=MUT_HIF1a_R170A_anti
     primer_bind     3941..3983
                     /label=MUT_HIF1a_R170A_sense
     misc_feature    3961..4026
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: Required for heterodimer formation with ARNT. 
                     {ECO:0000250|UniProtKB:Q61221}"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     primer_bind     4010..4041
                     /label=GD3473_MUT_HIF1a_V191D_sense
     primer_bind     complement(4010..4041)
                     /label=GD3474_MUT_HIF1a_V191D_anti
     exon            4024..4226
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(4032..4041)
                     /label=GMD-ASH/3'BspEI_NOstop_OR3
     primer_bind     4182..4201
                     /label=hHIF1a_fwdSEQ1
     misc_feature    4192..4194
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="Phosphoserine, by CK1. 
                     {ECO:0000269|PubMed:20699359}; propagated from 
                     UniProtKB/Swiss-Prot (Q16665.1); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            4227..4333
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     exon            4334..4481
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     exon            4482..4702
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(4519..4542)
                     /label=GMD-YC/(IRES)-HIF1A 363_stop_GArev
     primer_bind     complement(4519..4542)
                     /label=GMD-YC/3'NotI_stop_HIF1a_363
     primer_bind     complement(4519..4542)
                     /label=GMD-YC/(EPAS1 366)-HIF1A 363_GArev
     primer_bind     4543..4573
                     /label=GMD-YC/(EPAS1 365)-HIF1A 364_Gafwd
     primer_bind     4543..4572
                     /label=(mglk)-HIF1a_364_GAfwd
     misc_feature    4591..4704
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: N-terminal VHL recognition site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    4654..5262
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: ODD"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    4657..4659
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="4-hydroxyproline. {ECO:0000269|PubMed:11566883}; 
                     propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     hydroxylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            4703..4989
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     primer_bind     4750..4769
                     /label=GD3497_hHIF1a_fwdSEQ3
     primer_bind     4760..4769
                     /label=GMD-CD/mPolr2a_gRNA_CTDnt_top
     exon            4990..5112
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     misc_feature    5044..5178
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: NTAD"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    5047..5049
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="N6-acetyllysine. {ECO:0000269|PubMed:24681946}; 
                     propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     acetylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    5104..5106
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="Phosphoserine, by GSK3-beta. 
                     {ECO:0000269|PubMed:20889502}; propagated from 
                     UniProtKB/Swiss-Prot (Q16665.1); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            5113..5546
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     misc_feature    5116..5118
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="Phosphothreonine, by GSK3-beta. 
                     {ECO:0000269|PubMed:20889502}; propagated from 
                     UniProtKB/Swiss-Prot (Q16665.1); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    5119..5169
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: C-terminal VHL recognition site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    5143..5145
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="4-hydroxyproline. {ECO:0000269|PubMed:11292861, 
                     ECO:0000269|PubMed:11566883, ECO:0000269|PubMed:12351678, 
                     ECO:0000269|PubMed:25974097}; propagated from 
                     UniProtKB/Swiss-Prot (Q16665.1); hydroxylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     primer_bind     5151..5170
                     /label=hHIF1a_fwdSEQ2
     misc_feature    5179..5808
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: ID"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    5179..5181
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="Phosphoserine, by PLK3. 
                     {ECO:0000269|PubMed:20889502}; propagated from 
                     UniProtKB/Swiss-Prot (Q16665.1); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    5218..5220
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="Phosphoserine, by GSK3-beta. 
                     {ECO:0000269|PubMed:20889502}; propagated from 
                     UniProtKB/Swiss-Prot (Q16665.1); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     primer_bind     5303..5312
                     /label=GD3965_(link)-NPM1_C_LHA_GArev
     primer_bind     5380..5411
                     /label=VF425_Hif1aCterm_SpeI_GibF
     misc_feature    5422..5424
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="Phosphoserine, by PLK3. 
                     {ECO:0000269|PubMed:20889502}; propagated from 
                     UniProtKB/Swiss-Prot (Q16665.1); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            5547..5655
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     misc_feature    5578..5580
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="N6-acetyllysine. {ECO:0000269|PubMed:24681946}; 
                     propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     acetylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    5605..5616
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: Nuclear localization signal. {ECO:0000255}"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            5656..5782
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(5752..5784)
                     /label=VF426_Hif1aCterm_GibR
     exon            5783..5934
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /inference="alignment:same species:2.1.0"
     CDS             5785..5931
                     /codon_start=1
                     /label=AD2
                     /translation="LACRLLGQSMDESGLPELTSYDCEVEAPIEGSRNLLQGEELLRAL
                     DQVN"
     primer_bind     5785..5806
                     /label=VF433_Hif1aAD2_GibF
     misc_feature    5794..5796
                     /label=AGA>GAC for R>D
     misc_feature    5809..5931
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     Region: CTAD"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     primer_bind     complement(5822..5868)
                     /label=VF429_Hif1aAD2QN>E_GibR
     misc_feature    5833
                     /label=C>G for Q>E
     primer_bind     5848..5890
                     /label=VF430_Hif1aAD2QN>E_GibF
     misc_feature    5851..5853
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="S-nitrosocysteine. {ECO:0000305|PubMed:12914934}; 
                     propagated from UniProtKB/Swiss-Prot (Q16665.1); 
                     nitrosylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    5860..5862
                     /gene="HIF1A"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; 
                     HIF1; HIF1-ALPHA; MOP1; PASD8"
                     /note="(3S)-3-hydroxyasparagine. 
                     {ECO:0000269|PubMed:12080085}; propagated from 
                     UniProtKB/Swiss-Prot (Q16665.1); hydroxylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    5860
                     /label=A>G for N>E
     misc_feature    5862
                     /label=T>G for N>E
     primer_bind     5872..5882
                     /label=GD3586_mAR_fwdSEQ
     misc_feature    5872
                     /label=C>G for Q>E
     misc_feature    5881..5883
                     /label=AGA>GAC for R>D
     primer_bind     complement(5908..5950)
                     /label=(IRES)-hHIF1a_GArev
     primer_bind     complement(5908..5937)
                     /label=3'NotI_hHIF1a
     primer_bind     complement(5908..5935)
                     /label=3'KpnI_hHIF1a
     primer_bind     complement(5908..5935)
                     /label=GMD/3'AscI_mHif1a
     primer_bind     complement(5910..5935)
                     /label=(pX-C1_BamHI)-STOP-HIF1a_GArev
     misc_feature    5911..5913
                     /label=AGA>GAC for R>D
     primer_bind     complement(5931..5950)
                     /label=GD3717_(IRES)-stop_V5_GArev
     primer_bind     complement(5932..5950)
                     /label=GMD-YC/(IRES)-HIF1A 363_stop_GArev
     primer_bind     complement(5933..5950)
                     /label=IRES_GArev
     primer_bind     5935..5963
                     /label=5'NotI_IRES
     primer_bind     5935..5945
                     /label=GMD-WD/5'BsiWI_PL_IRES
     primer_bind     5936..5948
                     /label=5'BamHI_XhoI-IRES
     misc_feature    5938..6513
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     5944..5967
                     /label=5'MluI_IRES
     primer_bind     5944..5963
                     /label=GMD-CC/IRES_GA_vector_fwd
     primer_bind     5944..5962
                     /label=5'BamHI_IRES
     primer_bind     5945..5967
                     /label=5'BglII_BsiWI_NotI_IRES
     primer_bind     complement(5957..5977)
                     /label=GD3409_IRES_GArev_2
     primer_bind     complement(6019..6038)
                     /label=IRES_REV_SEQ
     primer_bind     complement(6080..6099)
                     /label=GMD/IRES_revSEQ_2
     primer_bind     6121..6130
                     /label=GMD-SC/RP11-1036K4_rev2
     primer_bind     complement(6183..6202)
                     /label=GD4070_IRES_mid_REV
     primer_bind     6233..6242
                     /label=GD3688_hNCOR1_IN_rev1
     primer_bind     6238..6289
                     /label=GMD-NW/3'BamHI(-NotI-XbaI-AgeI)_L30prom
     primer_bind     6278..6290
                     /label=GMD-LD/(PB)-L30prom_GArev
     primer_bind     6278..6289
                     /label=GMD-JK/L30prom_GArev_vector
     primer_bind     complement(6379..6401)
                     /label=VF428_IRES_KpnI_GibR
     primer_bind     complement(6396..6414)
                     /label=GD4187_IRES_GArev_3
     primer_bind     6411..6429
                     /label=IRES_FOR_SEQ
     primer_bind     complement(6490..6527)
                     /label=3'_IRES_NdeI_CFP
     primer_bind     complement(6490..6521)
                     /label=3'MluI_IRES
     primer_bind     complement(6501..6523)
                     /label=3'MfeI_IRES
     primer_bind     complement(6504..6527)
                     /label=550/3'NdeI_IRES
                     /note="GGAATTCCATATGTGTGGCCATATTATCATC"
     primer_bind     complement(6504..6522)
                     /label=ires-puro
     CDS             6528..7127
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6528..6548
                     /label=GMD-WD/5'NgoMIV_(P2A)_Puro
     primer_bind     6528..6548
                     /label=GMD-SC/5'HindIII_kozac_Puro
     primer_bind     6528..6548
                     /label=GD3712_5'AgeI_Puro
     primer_bind     complement(6528..6548)
                     /label=GD4134_Puro_REV
     primer_bind     6661..6671
                     /label=ctnnb1_screeningR
     primer_bind     complement(6867..6876)
                     /label=GD4175_(mglk)-noSTOP_hRXRA_GArev
     primer_bind     complement(6867..6876)
                     /label=GD4280_(pcDNA3.1-PmeI)- stop-RXRA_GArev
     primer_bind     complement(6895..6905)
                     /label=GMD/pX-C1_vector_GA_rev
     primer_bind     complement(6909..6918)
                     /label=GMD-YC/ESPAS1_NT_gRNA#1_anti
     primer_bind     complement(6971..6980)
                     /label=GD4350_hCDK9_N_LHArev_GA_V5
     primer_bind     complement(6971..6980)
                     /label=GD4347_hCDK9_N_LHArev_GA_3XF
     primer_bind     complement(6974..6986)
                     /label=GMD-CoV19/MUT_TAQ M747K_rev
     primer_bind     complement(7088..7107)
                     /label=3'NotI_PuroR
     primer_bind     complement(7110..7127)
                     /label=GMD-SC3'NotI_Puro_ver3
     primer_bind     complement(7111..7140)
                     /label=3'SalI_(ClaI)_PuroR
     primer_bind     complement(7111..7132)
                     /label=GMD-ASH/3'XhoI_Puro
     primer_bind     7135..7157
                     /label=GMD-Sheila/PB_GA_vector_FWD
     primer_bind     complement(7215..7238)
                     
     primer_bind     7215..7238
                     /label=GMD-ASH/646_gPCR-KIN_FWD1
     primer_bind     complement(7215..7234)
                     /label=564/wPRE rev seq
     polyA_signal    7255..7376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7348..7378)
                     /label=GD3580_(AASV1-RHA)-SV40_GArev
     primer_bind     complement(7353..7387)
                     /label=attB1_G-SV40polyA_op_5'
     primer_bind     complement(7353..7387)
                     /label=attB5_G-SV40polyA_op_5'
     primer_bind     complement(7353..7387)
                     /label=5'KpnI_G-SV40polyA_op
     primer_bind     complement(7353..7376)
                     /label=GMD-ST/3'XhoI_SV40 poly(A)
     primer_bind     complement(7353..7376)
                     /label=GD4327_(CMV)-SV40 polyA_GArev
     primer_bind     complement(7355..7386)
                     /label=GMD-ASH/3'FseI_SV40 poly(A)
     primer_bind     7359..7386
                     /label=GMD-ASH/646_gPCR-KIN_FWD2
     primer_bind     complement(7362..7387)
                     /label=GMD/3'ApaI_SV40 sti/polyA
     primer_bind     7649..7658
                     /label=JE19/eSpCas9s.seq4
     primer_bind     complement(8053..8062)
                     /label=GMD-YC/Strep_fwd_GA
     primer_bind     complement(8059..8072)
                     /label=VF184_hActg1_geneF
     repeat_region   8136..8170
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8158..8183)
                     /label=GMD/blunt_PiggyBac_FWD/REV
     primer_bind     complement(8186..8208)
                     /label=GMD/3'blunt(SwaI)_PiggyBac_REV
     primer_bind     complement(8186..8207)
                     /label=GMD/3'SmaI_PiggyBac_REV
     primer_bind     8225..8237
                     /label=5'NotI_1XF-mTAF9B
     primer_bind     8225..8237
                     /label=5'NotI_mTAF9B
     primer_bind     8225..8237
                     /label=5'SpeI_mTAF9b
     primer_bind     8225..8237
                     /label=5'XbaI_2_mTAF9b
     primer_bind     8225..8237
                     /label=5'XbaI_mTAF9b
     primer_bind     8381..8390
                     /label=GMD-CD/hPOLR2A_NT_gRNA#3_top
     primer_bind     8470..8479
                     /label=5'add_Ala_2L
     primer_bind     8470..8479
                     /label=5'delete_A_2L
     primer_bind     8549..8558
                     /label=GD4175_(mglk)-noSTOP_hRXRA_GArev
     primer_bind     8549..8558
                     /label=GD4280_(pcDNA3.1-PmeI)- stop-RXRA_GArev
     primer_bind     8642..8674
                     /label=GMD/MUT_null_lacZ_bot
     primer_bind     complement(8642..8674)
                     /label=GMD/MUT_null_lacZ_top
     primer_bind     complement(8661..8677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8674..8696)
                     /label=GMD/M13_REV_Yick
     protein_bind    8685..8701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8709..8739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     8713..8729
                     /label=GWc-R_attR1jct
     protein_bind    8754..8775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     8806..8823
                     /label=GD3625_(NLS)-LacI_GArev
     primer_bind     8806..8823
                     /label=GD3942_((SV40nls)-LacI_GArev
     primer_bind     complement(8878..8887)
                     /label=GMD/5'KpnI_T7prom-50
     primer_bind     complement(8887..8906)
                     /label=pQE SEQ1
     primer_bind     complement(8887..8896)
                     /label=5'BamHI_1XF_mTAF7L
     primer_bind     9019..9037
                     /label=GMD/pX-C1_vector_GA_rev
     primer_bind     complement(9019..9036)
                     /label=GD4095_(pX-C1)-L30_GAfwd
     primer_bind     complement(9026..9045)
                     /label=GMD/pEGFP minusCMV forSEQ
     primer_bind     9050..9059
                     /label=GMD-NW/mCdx2_OUTrev
     rep_origin      complement(9063..9650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9101..9120)
                     /label=GMD-TG/pCaSpeR_seq_02
     primer_bind     9154..9163
                     /label=GD3379_SP1delA_gRNA#1_anti
     primer_bind     9677..9696
                     /label=pQE SEQ2
     primer_bind     9687..9706
                     /label=LZ6 REV SEQ
     primer_bind     complement(9808..9820)
                     /label=3'M_NotI_pGL4
     CDS             complement(join(9821..9963,1..718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGTGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9939..9949
                     /label=GD4295_mSox9_MUT_gRNA#1_GArev
ORIGIN
        1 acccacgctc accggttcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1021 ggactccaac gtcaaaggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     1081 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     1141 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     1201 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     1261 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     1321 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     1381 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggtttcc cagtcacgac
     1441 gttgtaaaac gacggccagt gagcgcgcct cgttcattca cgtttttgaa cccgtggagg
     1501 acgggcagac tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg aagatgctcg
     1561 acacgctgca gaacacgcag ctagattaac cctagaaaga taatcatatt gtgacgtacg
     1621 ttaaagataa tcatgtgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt
     1681 atttattaat ttgaatagat attaagtttt attatattta cacttacata ctaataataa
     1741 attcaacaaa caatttattt atgtttattt atttattaaa aaaaacaaaa actcaaaatt
     1801 tcttctataa agtaacaaaa cttttatgag ggacagcccc cccccaaagc ccccagggat
     1861 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
     1921 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
     1981 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
     2041 tggggggata cggggaaaag gcctccacgg ccaaggatct gcgatcgcgt cgcagaacaa
     2101 acgcccagaa ccgaaggccg gagcccagac gccgcagcag cccagcctgc gttgtaaccc
     2161 ggtccccata gcaaccaact accgcagact actctcgcaa gcgctgttct gcttgtgacc
     2221 cggaccggct tctcttccgc ttccggtccc acaatcctct gtcggcctag aagagctttg
     2281 cattgtggga gctccttcct ttctcgctcc ccggccatct tggcggctgg tgttggtgag
     2341 tgagctctgc ggggtaaacg attaggcggc tcggggagct ccgctagctg gtgtttgacg
     2401 ctctggatta taatcctctc ttctgtcctc tgtgtatgct aggttggggg ttgtcccgca
     2461 cctagcggta cagccaccat gggtaagcct atccctaacc ctctcctcgg tctcgattct
     2521 accggaggct ccgcagaaat cggtactggc tttccattcg acccccatta tgtggaagtc
     2581 ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc
     2641 ctgcacggta acccgacctc ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg
     2701 acccatcgct gcattgctcc agacctgatc ggtatgggca aatccgacaa accagacctg
     2761 ggttatttct tcgacgacca cgtccgcttc atggatgcct tcatcgaagc cctgggtctg
     2821 gaagaggtcg tcctggtcat tcacgactgg ggctccgctc tgggtttcca ctgggccaag
     2881 cgcaatccag agcgcgtcaa aggtattgca tttatggagt tcatccgccc tatcccgacc
     2941 tgggacgaat ggccagaatt tgcccgcgag accttccagg ccttccgcac caccgacgtc
     3001 ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc
     3061 gtccgcccgc tgactgaagt cgagatggac cattaccgcg agccgttcct gaatcctgtt
     3121 gaccgcgagc cactgtggcg cttcccaaac gagctgccaa tcgccggtga gccagcgaac
     3181 atcgtcgcgc tggtcgaaga atacatggac tggctgcacc agtcccctgt cccgaagctg
     3241 ctgttctggg gcaccccagg cgttctgatc ccaccggccg aagccgctcg cctggccaaa
     3301 agcctgccta actgcaaggc tgtggacatc ggcccgggtc tgaatctgct gcaagaagac
     3361 aacccggacc tgatcggcag cgagatcgcg cgctggctgt cgacgctcga gatttccggc
     3421 agcggagatg gcgctggact gatcaacggc tcgatggagg gcgccggcgg cgcgaacgac
     3481 aagaaaaaga taagttctga acgtcgaaaa gaaaagtctc gagatgcagc cagatctcgg
     3541 cgaagtaaag aatctgaagt tttttatgag cttgctcatc agttgccact tccacataat
     3601 gtgagttcgc atcttgataa ggcctctgtg atgaggctta ccatcagcta tttgcgtgtg
     3661 aggaaacttc tggatgctgg tgatttggat attgaagatg acatgaaagc acagatgaat
     3721 tgcttttatt tgaaagcctt ggatggtttt gttatggttc tcacagatga tggtgacatg
     3781 atttacattt ctgataatgt gaacaaatac atgggattaa ctcagtttga actaactgga
     3841 cacagtgtgt ttgattttac tcatccatgt gaccatgagg aaatgagaga aatgcttaca
     3901 cacagaaatg gccttgtgaa aaagggtaaa gaacaaaaca cacagcgaag cttttttctc
     3961 agaatgaagt gtaccctaac tagccgagga agaactatga acataaagtc tgcaacatgg
     4021 aaggtattgc actgcacagg ccacattcac gtatatgata ccaacagtaa ccaacctcag
     4081 tgtgggtata agaaaccacc tatgacctgc ttggtgctga tttgtgaacc cattcctcac
     4141 ccatcaaata ttgaaattcc tttagatagc aagactttcc tcagtcgaca cagcctggat
     4201 atgaaatttt cttattgtga tgaaagaatt accgaattga tgggatatga gccagaagaa
     4261 cttttaggcc gctcaattta tgaatattat catgctttgg actctgatca tctgaccaaa
     4321 actcatcatg atatgtttac taaaggacaa gtcaccacag gacagtacag gatgcttgcc
     4381 aaaagaggtg gatatgtctg ggttgaaact caagcaactg tcatatataa caccaagaat
     4441 tctcaaccac agtgcattgt atgtgtgaat tacgttgtga gtggtattat tcagcacgac
     4501 ttgattttct cccttcaaca aacagaatgt gtccttaaac cggttgaatc ttcagatatg
     4561 aaaatgactc agctattcac caaagttgaa tcagaagata caagtagcct ctttgacaaa
     4621 cttaagaagg aacctgatgc tttaactttg ctggccccag ccgctggaga cacaatcata
     4681 tctttagatt ttggcagcaa cgacacagaa actgatgacc agcaacttga ggaagtacca
     4741 ttatataatg atgtaatgct cccctcaccc aacgaaaaat tacagaatat aaatttggca
     4801 atgtctccat tacccaccgc tgaaacgcca aagccacttc gaagtagtgc tgaccctgca
     4861 ctcaatcaag aagttgcatt aaaattagaa ccaaatccag agtcactgga actttctttt
     4921 accatgcccc agattcagga tcagacacct agtccttccg atggaagcac tagacaaagt
     4981 tcacctgagc ctaatagtcc cagtgaatat tgtttttatg tggatagtga tatggtcaat
     5041 gaattcaagt tggaattggt agaaaaactt tttgctgaag acacagaagc aaagaaccca
     5101 ttttctactc aggacacaga tttagacttg gagatgttag ctccctatat cccaatggat
     5161 gatgacttcc agttacgttc cttcgatcag ttgtcaccat tagaaagcag ttccgcaagc
     5221 cctgaaagcg caagtcctca aagcacagtt acagtattcc agcagactca aatacaagaa
     5281 cctactgcta atgccaccac taccactgcc accactgatg aattaaaaac agtgacaaaa
     5341 gaccgtatgg aagacattaa aatattgatt gcatctccat ctcctaccca catacataaa
     5401 gaaactacta gtgccacatc atcaccatat agagatactc aaagtcggac agcctcacca
     5461 aacagagcag gaaaaggagt catagaacag acagaaaaat ctcatccaag aagccctaac
     5521 gtgttatctg tcgctttgag tcaaagaact acagttcctg aggaagaact aaatccaaag
     5581 atactagctt tgcagaatgc tcagagaaag cgaaaaatgg aacatgatgg ttcacttttt
     5641 caagcagtag gaattggaac attattacag cagccagacg atcatgcagc tactacatca
     5701 ctttcttgga aacgtgtaaa aggatgcaaa tctagtgaac agaatggaat ggagcaaaag
     5761 acaattattt taataccctc tgatttagca tgtagactgc tggggcaatc aatggatgaa
     5821 agtggattac cagagctgac cagttatgat tgtgaagttg aggctcctat agaaggcagc
     5881 agaaacctac tgcagggtga agaattactc agagctttgg atcaagttaa ctgagcgccc
     5941 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     6001 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     6061 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     6121 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     6181 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     6241 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     6301 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     6361 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     6421 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     6481 cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatgatg accgagtaca
     6541 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6601 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6661 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6721 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6781 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6841 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6901 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6961 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7021 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7081 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaatc gatgtcgaca
     7141 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     7201 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgcgtta actaaacttg
     7261 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7321 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7381 gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctcatctg gtctcccttc
     7441 cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta
     7501 tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc
     7561 taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc
     7621 cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     7681 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     7741 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     7801 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     7861 ccctgatatc tataacaaga aatatatata taataagtta tcacgtaagt agaacatgaa
     7921 ataacaatat aattatcgta tgagttaatc ttaaagtcac gtaaaagata atcatgcgtc
     7981 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
     8041 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
     8101 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
     8161 ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag
     8221 tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg
     8281 cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga
     8341 gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg
     8401 tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt
     8461 ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg
     8521 ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga
     8581 cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg
     8641 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8881 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8941 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9001 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9541 accttcggaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9961 gag
//
