LOCUS       Exported                8736 bp ds-DNA     circular SYN 12-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Tjian Darzacq Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(719..823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(849..1304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(916..941)
                     /label=3'SacI_C-SV40polyA
     primer_bind     1443..1462
                     /label=GMD/pPthc_REVseq
     primer_bind     1446..1462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1579..1604
                     /label=GMD/blunt_PiggyBac_FWD/REV
     misc_feature    1592..1604
                     /label=PB Terminal repeat
     misc_feature    1639..1654
                     /label=IR (inverted repeat) truncated?
     primer_bind     2063..2093
                     /label=5'NruI_MluI_PGK_promoter
     promoter        2073..2572
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     2505..2525
                     /label=GMD/PGKprom_FWDseq2
     primer_bind     2584..2614
                     /label=5'HindIII_kozac_Puro
     CDS             2594..3193
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3176..3199)
                     /label=3'NotI_Puro_ver3
     primer_bind     3206..3223
                     /label=GMD-TH/pcDNA3.1_vector_GA_FWD
     primer_bind     complement(3220..3237)
                     /label=pcDNA3.1/BGH rev
     polyA_signal    3226..3450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3404..3423
                     /label=GMD-SC/bGHpolyA_fwdSEQ
     primer_bind     complement(3443..3470)
                     /label=3'AsiSI__bGH poly(A)
     promoter        3489..3864
                     /label=L30 prom
                     /note="L30 prom"
     primer_bind     3734..3753
                     /label=ES_SH_009/L30prom_forSEQ
     primer_bind     3734..3752
                     /label=prAH331
     primer_bind     complement(3846..3868)
                     /label=3'AvrII_L30prom
     primer_bind     3860..3895
                     /label=VF462_PB_l30_AD_GibsonF1
     primer_bind     3877..3895
                     /label=VF417_PB_AD_GibsonF1
     promoter        3877..3881
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     regulatory      3887..3896
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     3894..3932
                     /label=VF509_synTF_SNAPv2_F
     CDS             3897..3901
                     /codon_start=1
                     /label=GDGAGLIN linker
                     /translation="S"
     primer_bind     3901..3922
                     /label=GD3645_(mglk)-SNAPf_GAfwd
     primer_bind     3903..3924
                     /label=5'AgeI_SNAPf
     CDS             3908..4423
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag2
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTYDSPLGKLLLSGCEQGLHRIYFVGNGQGEQGPPG
                     PEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISY
                     SQLAALAGNPAATAAVKTALRGNPVPILIPCHRVVQGDGDVGGYEGPLYVKEWLLAHEG
                     HRLGKPGLG"
     misc_difference 3941..3943
                     /label=Y12
     misc_difference 3965..3967
                     /label=L20
     primer_bind     complement(3968..3986)
                     /label=GD4755_SNAP/CLIP_revSEQ
     misc_difference 4001..4003
                     /label=Y32
     misc_feature    4007..4009
                     /label=V34
     misc_feature    4013..4015
                     /label=N36
     misc_difference 4019..4039
                     /label=QGEQGPP
     misc_feature    4223..4225
                     /label=Q106
     misc_difference 4280..4282
                     /label=R125
     primer_bind     4304..4323
                     /label=SNAP-CLIP ForwardSEQ
     misc_feature    4334..4336
                     /label=G143
     misc_difference 4358..4360
                     /label=P151
     misc_difference 4364..4366
                     /label=Y153
     primer_bind     4366..4385
                     /label=1559/SNAP/CLIP_fwd_END
     primer_bind     complement(4408..4432)
                     /label=VF510_synTF_SNAPv2_R
     primer_bind     4413..4439
                     /label=VF511_synTF_ERD_F
     CDS             4421..4422
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation=""
     primer_bind     complement(4424..4458)
                     /label=VF418_PB_AD_GibsonR1
     CDS             4433..5317
                     /codon_start=1
                     /locus_tag="Estrogen Binding Domain"
                     /label=Estrogen Binding Domain
                     /translation="SAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPI
                     LYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLE
                     ILMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEE
                     FVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQR
                     LAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEE
                     TDQSHLATAGSTSS"
     primer_bind     5303..5325
                     /label=VF419_PB_ZFs_GibsonF2
     primer_bind     5303..5325
                     /label=VF422_PB_ZFonly_GibsonF2
     primer_bind     complement(5304..5329)
                     /label=VF512_synTF_ERD_R
     CDS             5324..5845
                     /codon_start=1
                     /locus_tag="ZF1-3"
                     /label=ZF1-3
                     /translation="SRPGERPFQCRICMRNFSEEANLRRHTRTHTGEKPFQCRICMRNF
                     SDHSSLKRHLRTHTGSQKPFQCRICMRNFSQSANLLRHTRTHTGEKPFQCRICMRNFSD
                     PSSLKRHLRTHTGSQKPFQCRICMRNFSQQTNLTRHTRTHTGEKPFQCRICMRNFSDAT
                     QLVRHLRTHLR"
     primer_bind     complement(5839..5917)
                     /label=VF420_PGK_PB_ZFs_GibsonR2
     primer_bind     complement(5839..5880)
                     /label=VF421_PB_IRES_ZFs_GibsonR2
     primer_bind     complement(5839..5878)
                     /label=VF463_PB_ZFs_GibsonR1
     CDS             5858..5899
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     5858..5879
                     /label=VF464_PB_V5_GibsonF2
     gene            5858..5859
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /label=Wapl
                     /note="WAPL cohesin release factor; Derived by automated 
                     computational analysis using gene prediction method: 
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     mRNA            5858..5859
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     X1"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 3 mRNAs, 179 ESTs, 583 long SRA 
                     reads, and 100% coverage of the annotated genomic feature 
                     by RNAseq alignments, including 18 samples with support for
                     all annotated introns"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     mRNA            5858..5859
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     1"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     mRNA            5858..5859
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="WAPL cohesin release factor, transcript variant 
                     2"
                     /label=Wapl mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     CDS             5858..5859
                     /codon_start=1
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="wings apart-like protein homolog isoform X1"
                     /label=Wapl
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
                     /protein_id="XP_006518887.1"
                     /translation=""
     CDS             5858..5859
                     /codon_start=1
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; BC037674; DIF-2; FOE; Wapal"
                     /product="wings apart-like protein homolog isoform 2"
                     /label=Wapl
                     /note="isoform 2 is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS79296.1"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
                     /protein_id="NP_001288259.1"
                     /translation=""
     primer_bind     complement(5875..5902)
                     
     primer_bind     complement(5876..5899)
                     /label=2928/V5_noSTOP_GArev
     primer_bind     complement(5881..5909)
                     /label=3'SalI_stop_V5
     exon            5899..5903
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /number=7
                     /inference="alignment:same species:2.1.0"
     mRNA            5899..5903
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /product="cyclin dependent kinase 9"
                     /label=CDK9 mRNA
                     /db_xref="GeneID:1025"
                     /db_xref="HGNC:HGNC:1780"
                     /db_xref="MIM:603251"
     misc_feature    5899
                     /gene="CDK9"
                     /gene_synonym="C-2k; CDC2L4; CTK1; PITALRE; TAK"
                     /note="propagated from UniProtKB/Swiss-Prot (P50750.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     primer_bind     complement(5984..6003)
                     /label=wPRE rev seq
     polyA_signal    6024..6145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6389..6410)
                     /label=VF465_PB_V5_GibsonR2
     misc_feature    complement(6908..6926)
                     /label=IR (inverted repeat)
     primer_bind     complement(6930..6955)
                     /label=GMD/blunt_PiggyBac_FWD/REV
     misc_feature    complement(6930..6942)
                     /label=PB Terminal repeat
     primer_bind     complement(7433..7449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7446..7468)
                     /label=GMD/M13_REV_Yick
     protein_bind    7457..7473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7481..7511)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     7485..7501
                     /label=GWc-R_attR1jct
     protein_bind    7526..7547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     7615..7658
                     /label=5'NruI_MluI_PGK_promoter
     primer_bind     complement(7659..7678)
                     /label=pQE SEQ1
     primer_bind     complement(7798..7817)
                     /label=GMD/pEGFP minusCMV forSEQ
     rep_origin      complement(7835..8423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8450..8469
                     /label=pQE SEQ2
     primer_bind     8460..8479
                     /label=LZ6 REV SEQ
     CDS             complement(join(8594..8736,1..718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1021 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1081 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1141 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1201 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1261 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     1321 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     1381 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1441 acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga
     1501 ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct
     1561 cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta
     1621 cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     1681 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     1741 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa
     1801 tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg
     1861 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     1921 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     1981 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     2041 cctgggggga tacggggaaa aggcgaacgc gtgggtaggg gaggcgcttt tcccaaggca
     2101 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     2161 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     2221 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     2281 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     2341 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     2401 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     2461 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     2521 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     2581 cccaagcttc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2641 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2701 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     2821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     2881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     2941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3181 gcccggtgcc tgagcggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     3241 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3301 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3361 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3421 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaacgcgat atgtacgggc
     3481 cagatatacg cgtcgcagaa caaacgccca gaaccgaagg ccggagccca gacgccgcag
     3541 cagcccagcc tgcgttgtaa cccggtcccc atagcaacca actaccgcag actactctcg
     3601 caagcgctgt tctgcttgtg acccggaccg gcttctcttc cgcttccggt cccacaatcc
     3661 tctgtcggcc tagaagagct ttgcattgtg ggagctcctt cctttctcgc tccccggcca
     3721 tcttggcggc tggtgttggt gagtgagctc tgcggggtaa acgattaggc ggctcgggga
     3781 gctccgctag ctggtgtttg acgctctgga ttataatcct ctcttctgtc ctctgtgtat
     3841 gctaggttgg gggttgtccc gcacctagag ctagcgccca gcttaagcca ccatgggatc
     3901 cggcagcatg gacaaagact gcgaaatgaa gcgcaccacc tacgatagcc ctctgggcaa
     3961 gctgctgctg tctgggtgcg aacagggcct gcaccgtatc tatttcgtgg gcaatggaca
     4021 gggagaacaa ggccctccag gaccagagcc actgatgcag gccaccgcct ggctcaacgc
     4081 ctactttcac cagcctgagg ccatcgagga gttccctgtg ccagccctgc accacccagt
     4141 gttccagcag gagagcttta cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt
     4201 cggagaggtc atcagctaca gccaactggc cgccctggcc ggcaatcccg ccgccaccgc
     4261 cgccgtgaaa accgccctga gaggaaatcc cgtgcccatt ctgatcccct gccaccgggt
     4321 ggtgcagggc gacggcgacg tggggggcta cgagggccct ctctacgtga aagagtggct
     4381 gctggcccac gagggccaca gactgggcaa gcctgggctg ggtagcgctg gctctgctgg
     4441 agacatgaga gctgccaacc tttggccaag cccgctcatg atcaaacgct ctaagaagaa
     4501 cagcctggcc ttgtccctga cggccgacca gatggtcagt gccttgttgg atgctgagcc
     4561 ccccatactc tattccgagt atgatcctac cagacccttc agtgaagctt cgatgatggg
     4621 cttactgacc aacctggcag acagggagct ggttcacatg atcaactggg cgaagagggt
     4681 gccaggcttt gtggatttga ccctccatga tcaggtccac cttctagaat gtgcctggct
     4741 agagatcctg atgattggtc tcgtctggcg ctccatggag cacccagtga agctactgtt
     4801 tgctcctaac ttgctcttgg acaggaacca gggaaaatgt gtagagggca tggtggagat
     4861 cttcgacatg ctgctggcta catcatctcg gttccgcatg atgaatctgc agggagagga
     4921 gtttgtgtgc ctcaaatcta ttattttgct taattctgga gtgtacacat ttctgtccag
     4981 caccctgaag tctctggaag agaaggacca tatccaccga gtcctggaca agatcacaga
     5041 cactttgatc cacctgatgg ccaaggcagg cctgaccctg cagcagcagc accagcggct
     5101 ggcccagctc ctcctcatcc tctcccacat caggcacatg agtaacaaag gcatggagca
     5161 tctgtacagc atgaagtgca agaacgtggt gcccctctat gacctgctgc tggagatgct
     5221 ggacgcccac cgcctacatg cgcccactag ccgtggaggg gcatccgtgg aggagacgga
     5281 ccaaagccac ttggccactg cgggctctac ttcatcgggt acctctagac caggcgaacg
     5341 accgtttcaa tgccggatat gtatgaggaa cttctccgag gaggcaaact tgaggcgcca
     5401 cacccgaaca catacaggag aaaagccatt ccaatgtcga atttgtatgc gcaatttttc
     5461 agatcactca agcctcaagc gacacctccg cacacatact ggttcacaga agccctttca
     5521 gtgcaggatt tgtatgcgca actttagcca atcagcgaac cttttgcggc acactagaac
     5581 gcatacaggt gagaagcctt tccagtgtcg catctgtatg cggaacttca gcgaccccag
     5641 ttcattgaag aggcatttgc gaactcacac cggttctcaa aaaccttttc agtgccgaat
     5701 ttgtatgcgc aacttcagcc aacaaacaaa tttgacgaga cacacgcgca cgcacaccgg
     5761 ggaaaaaccg tttcaatgtc gaatctgcat gcgcaatttt agcgatgcga cacaacttgt
     5821 taggcatctg cgcacacact tgcggggaag cggagctggt aagcctatcc ctaaccctct
     5881 cctcggtctc gattctacct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     5941 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6001 gcctttgtat catgcgttaa ctaaacttgt ttattgcagc ttataatggt tacaaataaa
     6061 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6121 tgtccaaact catcaatgta tcttatcatg tctggaattg actcaaatga tgtcaattag
     6181 tctatcagaa gctcatctgg tctcccttcc gggggacaag acatccctgt ttaatattta
     6241 aacagcagtg ttcccaaact gggttcttat atcccttgct ctggtcaacc aggttgcagg
     6301 gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag tggcagccag gtttagcccc
     6361 ggaattgact ggattccttt tttagggccc attggtatgg ctttttcccc gtatcccccc
     6421 aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac
     6481 cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg
     6541 gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta
     6601 catccctggg ggctttgggg gggggctgtc cctgatatct ataacaagaa aatatatata
     6661 taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat
     6721 cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc
     6781 aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact
     6841 gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc
     6901 aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatcta gctgcatcag
     6961 gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc
     7021 atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt
     7081 gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt
     7141 cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat
     7201 accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc
     7261 aacccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc
     7321 ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa
     7381 tggacatgga taccccgtga gttacccggc gggcgcgctt ggcgtaatca tggtcatagc
     7441 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7501 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7561 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7621 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7681 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7741 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7801 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7861 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7921 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7981 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8041 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8101 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8161 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8221 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8281 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8341 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8401 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8461 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8521 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8581 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8641 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8701 taccatctgg ccccagtgct gcaatgatac cgcgag
//
