LOCUS       pColE1-MP-mRFP1         3753 bp ds-DNA     circular     19-MAY-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            206802-attachment_w6NuwqrDhQjDmcOzRRY.gb
FEATURES             Location/Qualifiers
     rep_origin      complement(1..683)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(778..1638)
                     /label="AmpR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(778..1638)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    complement(1639..1743)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2181..2304
                     /label="J3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2305..2324
                     /label="DA9 spacer target"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2325..2350
                     /label="UP-1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2351..2385
                     /label="MP-1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2386..2412
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     RBS             2395..2406
                     /label="RBS (strong)"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     misc_feature    2413..3090
                     /label="mRFP1"
                     /ApEinfo_revcolor="#ff3737"
                     /ApEinfo_fwdcolor="#ff3737"
     CDS             2413..3090
                     /label="mRFP1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTGA*"
ORIGIN
        1 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
       61 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
      121 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
      181 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC
      241 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
      301 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
      361 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
      421 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
      481 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
      541 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
      601 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
      661 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
      721 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
      781 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
      841 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
      901 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
      961 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     1021 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
     1081 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
     1141 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
     1201 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
     1261 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
     1321 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
     1381 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
     1441 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
     1501 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
     1561 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
     1621 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
     1681 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
     1741 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT
     1801 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTT CAAGAATTCT GGCGAATCCT
     1861 CTGACCAGCC AGAAAACGAC CTTTCTGTGG TGAAACCGGA TGCTGCAATT CAGAGCGGCA
     1921 GCAAGTGGGG GACAGCAGAA GACCTGACCG CCGCAGAGTG GATGTTTGAC ATGGTGAAGA
     1981 CTATCGCACC ATCAGCCAGA AAACCGAATT TTGCTGGGTG GGCTAACGAT ATCCGCCTGA
     2041 TGCGTGAACG TGACGGACGT AACCACCGCG ACATGTGTGT GCTGTTCCGC TGGGCATGCC
     2101 AGGACAACTT CTGGTCCGGT AACGTGCTGA GCCCGGCCAA GCTTAGCccg aaaagtgcca
     2161 cctgTGGCAA TTCCGACGTC AGCATTTGCG ATCATTCACG CAGCGCTTAT TCAGTTGCTC
     2221 ACTGCGATGT CATAATCATC GCTACGAGCT GTGAAAGATG CATAAAGCTC GTACGACGCG
     2281 TTCGCTCGTC TCCTCACTTC TCCTAACTCT CACACGTGGC TGCACCGGCC CCCCCCGCTG
     2341 CCGCGGGCCG TTGACAGTTT CGGGGCCGAA AGGGATTGTA CGAGCGAATT CATTAAAGAG
     2401 GAGAAAGGTA CCATGGCGAG TAGCGAAGAC GTTATCAAAG AGTTCATgcg tttcaaagtt
     2461 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt
     2521 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc
     2581 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg
     2641 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt
     2701 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt
     2761 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg
     2821 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct
     2881 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa
     2941 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac
     3001 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt
     3061 gctgaaggtc gtcactccac cggtgcttaa ggatccaaac tcgagtaagg atctGTGCTT
     3121 TTTTTaacgc atgagAAAGC CCCCGGAAGA TCACCTTCCG GGGGCTTTtt tattgcgcGG
     3181 ATCCTTACTC CGTCGACAAG CTTGCGGCCG CACTCGAGGA ATTCGACTCA ATTAGTTCAG
     3241 TCAGTTTCAG GATATTAGTC ATCTCTACAT TGATTATGAG TATTCAGAAA TTCCTTAAAT
     3301 ATTCTGACAA ATGCTCTTTC CCTAAACTCC CCCCATAAAA AAACCCGCCG AAGCGGGTTT
     3361 TTACGTTATT TGCGGATTAA CGATTACTCG TTATCAGAAC CGCCCAGACC TGCGTTCAGC
     3421 AGTTCTGCCA GGCTGGCAGA TGCGTCTTCC GAATTGATCC GTCGACCGAT GCCCTTGAGA
     3481 GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT
     3541 ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT CTTCCGCTTC
     3601 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
     3661 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
     3721 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAA
//